阅读说明：本技术 一种血浆的凝胶处理设备及方法 (Gel treatment equipment and method for plasma ) 是由 栗云天 王洪春 于 2020-12-14 设计创作，主要内容包括：本发明涉及一种血浆的凝胶处理设备及方法,将凝胶置于有机玻璃柱内,并将血浆从顶部或底部泵入柱内；利用搅拌设备将血浆和凝胶进行特殊的混合搅拌,使血浆中的凝血酶原复合物被充分吸附到填料中,然后经过滤网抽出血浆至血浆罐；将洗涤液从顶部或底部泵入柱内,进行清洗搅拌,去除填料中残留的血浆和杂质,然后通过滤网抽出清洗液；抽出洗脱液及洗脱的凝血酶原复合物,至制品罐；至此完成了一个工艺循环。本发明系统密闭,无污染的风险,不需要多个罐体,所有操作都在有机玻璃柱内完成,通过特殊设计的搅拌装置帮助凝胶和液体透过筛网进行分离,大大提高了透过速度,摆脱了手动操作,容易实现自动化。(The invention relates to a plasma gel processing device and a plasma gel processing method, wherein gel is placed in an organic glass column, and plasma is pumped into the column from the top or the bottom; the plasma and the gel are mixed and stirred specially by using stirring equipment, so that a prothrombin compound in the plasma is fully absorbed into a filler, and then the plasma is pumped out through a filter screen to a plasma tank; pumping the cleaning solution into the column from the top or the bottom, cleaning and stirring to remove residual plasma and impurities in the filler, and then pumping out the cleaning solution through a filter screen; extracting the eluent and the eluted prothrombin complex to a product tank; this completes a process cycle. The system is closed, no pollution risk exists, a plurality of tank bodies are not needed, all operations are completed in the organic glass column, the gel and the liquid are separated by the aid of the stirring device with special design through the screen, the permeation speed is greatly improved, manual operation is avoided, and automation is easy to realize.)

1. A method for gel processing of plasma, comprising the steps of:

s1, feeding, namely placing the gel into an organic glass column, pumping the plasma into the column from the top or the bottom or feeding the gel into a plasma tank, stirring and adsorbing the plasma and pumping the plasma into the organic glass column;

s2 adsorption, specifically mixing and stirring the plasma and the gel by using stirring equipment to ensure that the prothrombin compound in the plasma is fully adsorbed into the filler, and then pumping the plasma to a plasma tank through a filter screen;

s3, washing, namely pumping washing liquid into the column from the top or the bottom, washing and stirring to remove residual blood plasma and impurities in the filler, and then pumping the washing liquid out through a filter screen;

s4, eluting, namely pumping the eluent into the column from the top or the bottom, mixing and stirring to strip the prothrombin complex adsorbed on the filler;

s5, obtaining a product, and extracting eluent and the eluted prothrombin compound to a product tank; this completes a process cycle.

2. The method for gel processing of plasma according to claim 1, wherein in S1, the gel is fed to a plexiglass column, and the plasma is pumped into the plexiglass column.

3. The method for gel processing of plasma according to claim 1, wherein in S2, the washing solution is pumped into the column from the top or the bottom, and after washing and stirring, the washing solution is filtered out through a stainless steel filter screen.

4. The method for gel processing of plasma according to claim 1, wherein in S3, the eluent is pumped into the column from the top or the bottom, after mixing and stirring, the product is eluted from the gel, and the eluent is filtered out by a stainless steel screen.

5. The method for gel processing of plasma according to claim 1, wherein the gel is discharged from the plexiglass column through the outlet at the bottom.

6. A device for the gel treatment of plasma for carrying out and implementing a method for the gel treatment of plasma according to any one of claims 1 to 5, characterized in that it comprises a plexiglas column, a pump body, a sieve and a stirring device.

7. The device for gel processing of plasma according to claim 6, wherein the stirring device is arranged inside the plexiglas column while gel is placed inside the plexiglas column.

8. The apparatus of claim 6, wherein the top and bottom of the plexiglass column are provided with through holes, and the filter screen is provided on the through holes at the bottom of the plexiglass column.

9. The apparatus for gel processing of plasma according to claim 8, wherein the plexiglas column is in a sealed state during operation, and the plexiglas column is a transparent plexiglas column in which the state of the gel can be observed.

10. The apparatus for gel processing of plasma according to claim 8, wherein the bottom port of the plexiglas column is connected to the plasma canister by a pump body.

Technical Field

The invention relates to the technical field of medical treatment, in particular to gel treatment equipment and a method for blood plasma.

Background

In the conventional purification process of prothrombin complex (before and after virus inactivation), DEAE Sephadex A-50 gel is generally put into a plasma tank and stirred, mixed thoroughly, and then pumped into a gel treatment device for filtration and separation (figure 1). The gel treatment device is an open container or a closed stainless steel tank with a layer of screen (nylon or stainless steel) on the bottom. Under the action of gravity, the gel and the plasma are separated, the plasma flows out through the screen, and the gel is stacked on the screen.

Because the gel itself is softer, easy deformation, can't let plasma permeate through by gravity after certain thickness is piled up to the gel, so some designs can increase a drawing liquid pump in the export, produce the vacuum and carry out the suction filtration. Because the plasma can not be filtered out by a suction filtration method when the gel accumulation thickness is too large, the plasma can be filtered out by manually stirring in an open container or stirring the gel at the upper layer by a stirring paddle in a closed tank body.

The gel after the plasma adsorption also needs to be put back into another processing container again for washing and elution, adsorbed impurities are removed by washing, and products adsorbed by the gel are eluted from the gel by eluent. Both methods also require separation of the liquid and gel through a container with a mesh at the bottom.

Because plasma impurities before inactivation are more and the screen is easy to block, the screen in the container needs to be replaced frequently.

Disclosure of Invention

Aiming at the defects of the prior art, the invention discloses a plasma gel treatment device and a plasma gel treatment method, which are used for solving the problems that most devices used in the prior gel filtration process are not closed and have the possibility of secondary pollution; even if the accumulation condition of gel in the container is not easy to observe only a window (or no window) in the closed container, the separation of liquid and gel is inconvenient, the adsorption of PCC, the washing and the elution of gel are carried out in different containers, the risk of breakage exists in a filter screen for filtering the gel, the product pollution is caused, the excessive accumulation of the gel cannot be avoided by simple in-tank stirring or manual stirring, the filtering speed is slow, the treatment time is long, the process cannot be automated, and the manual operation is needed.

The invention is realized by the following technical scheme:

in a first aspect, the present invention discloses a method for gel treatment of plasma, said method comprising the steps of:

s1, feeding, namely placing the gel into an organic glass column, pumping the plasma into the column from the top or the bottom or feeding the gel into a plasma tank, stirring and adsorbing the plasma and pumping the plasma into the organic glass column;

s2 adsorption, specifically mixing and stirring the plasma and the gel by using stirring equipment to ensure that the prothrombin compound in the plasma is fully adsorbed into the filler, and then pumping the plasma to a plasma tank through a filter screen;

s3, washing, namely pumping washing liquid into the column from the top or the bottom, washing and stirring to remove residual blood plasma and impurities in the filler, and then pumping the washing liquid out through a filter screen;

s4, eluting, namely pumping the eluent into the column from the top or the bottom, mixing and stirring to strip the prothrombin complex adsorbed on the filler;

s5, obtaining a product, and extracting eluent and the eluted prothrombin compound to a product tank; this completes a process cycle.

Further, in S1, the gel is put into a plasma tank, stirred and adsorbed, and then pumped into a plexiglass column.

Further, in the step S2, the washing solution is pumped into the column from the top or the bottom, and after washing and stirring, the washing solution is filtered out by a stainless steel screen.

Further, in S3, the eluent is pumped into the column from the top or the bottom, and after mixing and stirring, the mixed solution is eluted from the gel, and the eluent is filtered out through a stainless steel screen.

Further, in the method, the gel can be discharged out of the organic glass column through a glue outlet at the bottom.

In a second aspect, the invention discloses a plasma gel processing device, which is used for executing and realizing the plasma gel processing method of the first aspect, and comprises an organic glass column, a pump body, a filter screen and a stirring device.

Further, the stirring device is arranged in the organic glass column, and gel is placed in the organic glass column.

Furthermore, organic glass capital portion and bottom all are equipped with the opening, be equipped with on the organic glass column bottom opening the filter screen.

Furthermore, the organic glass column is in a sealing state during working, and the organic glass column is in a transparent organic glass column and can observe the state of gel.

Furthermore, the bottom port of the organic glass column is connected with the plasma tank through the pump body.

The invention has the beneficial effects that:

the system is closed, no pollution risk exists, and the previous adsorption, washing and elution are required to be carried out in different tanks.

Except for the first feeding, all the process steps are finished in the organic glass column, so that a transfer tank body is saved, the gel does not need to be moved out of the column body (or only move between the gel column and the plasma tank), the gel and the liquid are assisted to be separated through a screen by a specially designed gel stirring device, the permeation speed is greatly improved, the manual operation is avoided, and the automation is easy to realize.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a diagram of a conventional purification process of prothrombin complex;

FIG. 2 is a schematic diagram of the gel processing method of plasma;

FIG. 3 is a schematic diagram of a device for gel processing of plasma.

The reference numerals in the drawings denote:

1. a machine glass column; 2. a pump body; 3. filtering with a screen; 4. stirring equipment, 5, ports.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

This example discloses a method for gel processing of plasma as shown in fig. 2, comprising the following steps:

s1, feeding, namely placing the gel into an organic glass column, pumping the plasma into the column from the top or the bottom or feeding the gel into a plasma tank, stirring and adsorbing the plasma and pumping the plasma into the organic glass column;

s2 adsorption, specifically mixing and stirring the plasma and the gel by using stirring equipment to ensure that the prothrombin compound in the plasma is fully adsorbed into the filler, and then pumping the plasma to a plasma tank through a filter screen;

s3, washing, namely pumping washing liquid into the column from the top or the bottom, washing and stirring to remove residual blood plasma and impurities in the filler, and then pumping the washing liquid out through a filter screen;

s4, eluting, namely pumping the eluent into the column from the top or the bottom, mixing and stirring to strip the prothrombin complex adsorbed on the filler;

s5, obtaining a product, and extracting eluent and the eluted prothrombin compound to a product tank; this completes a process cycle.

In this example S1, the gel was put into a plasma tank, stirred and adsorbed, and then pumped into a plexiglass column.

In this example S2, the washing solution was pumped into the column from the top or bottom, and after washing and stirring, it was filtered off through a stainless steel screen.

In this example, S3, the eluent was pumped into the column from the top or bottom, and after mixing and stirring, the mixture was eluted from the gel, followed by filtration of the eluent through a stainless steel screen.

In this embodiment, the gel can be discharged from the plexiglass column through the glue outlet at the bottom.

The plasma and gel of this example were separated by valving and agitation in a closed dedicated gel processing apparatus. The gel state was observed in a sealed transparent plexiglas column. The adsorption of the product in the plasma, the washing of the gel and the elution of the product are all carried out in an organic glass column. The gel and liquid are separated through a stainless steel mesh by a special agitation. The manual operation is avoided, the pollution is avoided, and the automation is easy to realize.

Example 2

The embodiment discloses a gel treatment process which integrates the processes of plasma adsorption, gel washing and product elution into an organic glass column and is carried out in a centralized way.

The gel is placed in an organic glass column, the plasma is pumped into the column from the top or the bottom, and is specially mixed and stirred with the gel, and the plasma is filtered out by a stainless steel filter screen after adsorption is finished and returns to a plasma tank.

The washing liquid is pumped into the column from the top or the bottom, and is filtered out by a stainless steel filter screen after being washed and stirred. The eluent is pumped into the column from the top or the bottom, and after mixing and stirring, the product is eluted from the gel and filtered out by a stainless steel screen along with the eluent.

If necessary, the gel can be discharged out of the plexiglass column through the glue outlet at the bottom. The whole process can realize automatic operation.

Example 3

The embodiment discloses a plasma gel processing device as shown in fig. 3, which comprises a plexiglass column, a pump body, a filter screen and a stirring device.

Stirring equipment sets up in the organic glass post, placed the gel in the organic glass post simultaneously.

Organic glass capital portion and bottom all are equipped with the opening, be equipped with on the organic glass capital portion opening the filter screen.

The organic glass column is in a sealing state during working, and the organic glass column is in a transparent organic glass column state in which gel can be observed.

The bottom port of the organic glass column is connected with the plasma tank through the pump body.

In summary, the system of the present invention is closed without risk of contamination, and the previous adsorption, washing and elution need to be performed in different tanks.

The invention does not need a plurality of tank bodies, all operations are finished in the organic glass column, and the gel and the liquid are separated by the special stirring device which is designed to help the gel and the liquid to permeate the screen, thereby greatly improving the permeation speed, getting rid of the manual operation and being easy to realize automation.

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.