阅读说明：本技术 核酸编码反应 (Nucleic acid encoding reactions ) 是由 梅甘·安德森 陈佩林 布赖恩·福勒 罗伯特·C·琼斯 菲奥娜·卡佩尔 罗纳德·列波夫斯基 于 2012-05-21 设计创作，主要内容包括：本申请涉及核酸编码反应。描述了可用于向一个、或典型地多个靶核苷酸序列掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列的方法。在具体实施方案中,产生了具有衔接子的核酸片段,例如适于用在高通量DNA测序中的核酸片段。在其他实施方案中,将有关反应混合物的信息编码到反应产物中。还描述了在制备用于应用诸如双向核酸测序中可用于扩增一个或多个靶核酸的方法和试剂盒。在具体实施方案中,本申请的方法包括另外进行双向DNA测序。还描述了通过引物延伸编码和检测和/或定量等位基因的方法。(The present application relates to nucleic acid encoding reactions. Methods are described that can be used to incorporate one or more adaptors and/or nucleotide tags and/or barcode nucleotide sequences into one, or typically a plurality of, target nucleotide sequences. In particular embodiments, nucleic acid fragments with adaptors, such as nucleic acid fragments suitable for use in high-throughput DNA sequencing, are generated. In other embodiments, information about the reaction mixture is encoded into the reaction product. Also described are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, the methods of the present application comprise additionally performing bidirectional DNA sequencing. Methods of encoding and detecting and/or quantifying alleles by primer extension are also described.)