阅读说明：本技术 基因文库的构建方法及其应用 (Construction method and application of gene library ) 是由 赵佳 杨林 卢森 蒲丹丹 高健 高雅 陈芳 于 2018-04-09 设计创作，主要内容包括：一种基因文库的构建方法,根据该方法构建得到的基因文库,以及在孕妇无创产前检测领域中的应用。该基因文库的构建方法包括将生物样本和肝素酶共孵育,获得含有生物样本和肝素酶的共孵育样本；从共孵育样本中获取得到DNA,进行高通量建库测序,其中建库过程中利用Taq酶对DNA进行扩增。(A construction method of a gene library, the gene library constructed by the method and application in the field of noninvasive prenatal detection of pregnant women. The construction method of the gene library comprises the steps of incubating a biological sample and heparinase together to obtain an incubated sample containing the biological sample and the heparinase; and obtaining DNA from the co-incubation sample, and performing high-throughput library construction sequencing, wherein Taq enzyme is used for amplifying the DNA in the library construction process.)