阅读说明：本技术 用于检测微生物的基于环介导等温扩增(lamp)的测定 (Loop-mediated isothermal amplification (LAMP) -based assays for detecting microorganisms ) 是由 黄啸 迈克尔·R·霍夫曼 于 2018-12-31 设计创作，主要内容包括：公开了一种用于检测疑似含有病原体的样品中的微生物病原体的方法和系统。该方法包括将环介导等温扩增(LAMP)试剂和聚合物凝胶(诸如水凝胶)与样品合并以形成混合物。使凝胶在短时间内聚合,将病毒颗粒固定在混合物内。如果样品中存在靶DNA/RNA,则产生扩增子。通过视觉检测扩增子的存在或不存在来检测靶微生物。靶微生物浓度可以基于反应后荧光扩增子点的数目使用智能手机或荧光显微镜确定。该方法可以用于快速且低廉地以高灵敏度对环境水样品中的微生物病原体定量。(A method and system for detecting a microbial pathogen in a sample suspected of containing the pathogen is disclosed. The method includes combining a loop-mediated isothermal amplification (LAMP) reagent and a polymer gel (such as a hydrogel) with a sample to form a mixture. The gel is allowed to polymerize in a short time, immobilizing the viral particles within the mixture. If the target DNA/RNA is present in the sample, an amplicon is produced. The target microorganism is detected by visually detecting the presence or absence of the amplicon. The target microorganism concentration can be determined using a smartphone or a fluorescence microscope based on the number of fluorescent amplicon points after the reaction. The method can be used to rapidly and inexpensively quantify microbial pathogens in environmental water samples with high sensitivity.)