阅读说明：本技术 解除对tba检测干扰的方法、tc试剂盒及tg试剂盒 (Method for removing interference on TBA detection, TC kit and TG kit ) 是由 龚婷 赵畅 吴年芬 凡速朋 梁艳 舒芹 张雪娇 赵愿安 于 2020-06-11 设计创作，主要内容包括：本发明涉及试剂盒技术领域,具体公开了一种解除对TBA检测干扰的方法,将对TBA检测存在干扰的试剂盒中的胆酸钠去掉,将酚类底物替换为苯胺类衍生物；并在对TBA检测存在干扰的试剂盒中加入表面活性剂。该方法能够保证试剂盒的准确性前提下,还能解除其对TBA检测的干扰,并能减少试剂的刺激性,减少对人体的伤害。(The invention relates to the technical field of kits, and particularly discloses a method for relieving interference on TBA detection, wherein sodium cholate in a kit which has interference on TBA detection is removed, and a phenol substrate is replaced by an aniline derivative; and a surfactant is added to the kit that interferes with the detection of TBA. The method can also relieve the interference of the kit on TBA detection on the premise of ensuring the accuracy of the kit, reduce the irritation of the reagent and reduce the harm to human bodies.)

1. A method for relieving interference on TBA detection is characterized in that sodium cholate in a kit which generates interference on TBA detection is removed, and a phenolic substrate is replaced by an aniline derivative; and a surfactant is added to the kit that interferes with the detection of TBA.

2. The method of claim 1, wherein the kit further comprises a proteinaceous or carbohydrate substance that interferes with the detection of TBA.

3. A kit for eliminating interference on TBA detection is characterized in that,

the R1 reagent of the kit comprises a protective agent, a substrate, a surfactant, an ionic substance and a preservative; wherein the substrate is aniline derivatives.

4. The kit according to claim 3, characterized in that the substrate is: TOOS, MAOS, TOPS, HDAOS, HALPS or HMMPS.

5. The kit according to claim 3, wherein the surfactant is: emullgen A90, Emulgen A60, Emulgen B66, Emullgen 209, Emulgen430, Emullgen 709, polyethylene glycol 2000, polyethylene glycol 4000, polyethylene glycol 6000, Tween-20, Tween-80, polyoxyethylene lauryl ether, glycerol, castor oil, Tritox-405, PVP-40, Triton-114, Triton-100, GENAPOLX-080 or NP-1055.

6. The kit of claim 3, wherein the ionic species comprises one or a combination of two of the following: disodium ethylene diamine tetraacetate, zinc sulfate, sodium chloride, potassium chloride, manganese dichloride, calcium chloride, ammonium chloride, magnesium sulfate, lithium sulfate, ammonium sulfate, magnesium acetate, sodium tetraprocate, potassium carbonate and sodium tartrate.

7. The kit of claim 3, wherein the kit is a TC kit;

the R1 reagent of the TC kit also comprises PTPES, cholesterol esterase, cholesterol oxidase and peroxidase;

the R2 reagent of the TC kit comprises PIPES, 4-aminoantipyrine, sodium azide and protein substances.

8. The kit according to claim 7, wherein, in the R1 reagent of the TC kit,

the protective agent is: aspartic acid, glycine, L-serine, oxalic acid, bovine serum albumin or sodium glutamate.

9. The kit according to claim 3, wherein the kit is a TG kit;

the R1 reagent of the TG kit also comprises HEPES, bile protein lipase, peroxidase, glycerol kinase and phosphoglycerol oxidase;

the R2 reagent of the TG kit comprises PIPES, 4-aminoantipyrine, sodium azide and protein substances.

10. The kit according to claim 9, wherein in the R1 reagent of the TG kit, the protecting agent is: sucrose, mannitol, trehalose, lactitol, sorbitol or glucose.

Technical Field

The invention relates to the technical field of kits, in particular to a method for removing interference on TBA detection, a TC kit and a TG kit.

Background

In order to ensure the accuracy of the measured value of the reagent, sodium cholate is added in the reagent of a total cholesterol (COD-CE-PAP method) measuring kit (TC kit) and a triglyceride (GPO-PAP method) measuring kit (TG kit), but when the reagent is detected on a biochemical analyzer, the sodium cholate in the reagent can interfere the measured value of the TBA reagent due to the unclean cleaning of a reaction cup or a sampling needle of the biochemical analyzer, and the result of the measured value of the TBA can be wrong. When a plurality of reagents of a biochemical analyzer are detected, cross contamination is a relatively complex and difficult-to-solve problem, and the total cholesterol (COD-CE-PAP method) (TC kit) and the triglyceride (GPO-PAP method) determination kit (TG kit) provided by the invention can eliminate interference brought by simultaneous detection with a TBA reagent; meanwhile, the problem that most reagents in the market have unpleasant pungent smell obviously after the reagent is uncovered due to the fact that the reagents contain the phenolic substrates with the pungent smell can be solved.

Disclosure of Invention

The invention provides a method for relieving interference on TBA detection, a TC kit and a TG kit, which can relieve the interference on TBA detection by TC detection or TG detection, improve the stability of TC and TG reagents, reduce the irritation of the reagents and reduce the harm to human bodies.

In order to realize the purpose, the invention is realized by the following technical scheme:

a method for relieving interference to TBA detection comprises removing sodium cholate in a kit which generates interference to TBA detection, and replacing a phenol substrate with an aniline derivative; and a surfactant is added to the kit that interferes with the detection of TBA.

A kit for removing interference on TBA detection, wherein the R1 reagent of the kit comprises a protective agent, a substrate, a surfactant, an ionic substance and a preservative; wherein the substrate is aniline derivatives.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a method for removing interference on TBA detection, which removes sodium cholate in a kit generating interference on TBA detection, and replaces a phenol substrate with an aniline derivative; and a surfactant is added to the kit that interferes with the detection of TBA. The method can also relieve the interference of the reagent kit on TBA detection, reduce the irritation of the reagent and reduce the harm to human body on the premise of ensuring the accuracy of the interfering reagent kit. Meanwhile, a protein protective agent or a carbohydrate protective agent is further added into the R1 reagent of the kit provided by the invention, so that the thermal stability of the kit can be improved.

Drawings

FIG. 1 is a graph of the mean interference bias for TBA detection without heat treatment of a TC kit provided in an embodiment of the invention;

FIG. 2 is a graph of the mean interference deviation for TBA detection after heat treatment of a TC kit provided in an embodiment of the present invention;

FIG. 3 is a graph showing the mean interference deviation of a TG kit provided in an example of the present invention against TBA detection without heat treatment;

FIG. 4 is a graph showing the mean interference deviation for TBA detection after heat treatment of the TG kit provided in the example of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

When the TBA kit is detected, if the kit which is detected on the same biochemical instrument simultaneously with the TBA kit contains sodium cholate, interference on the TBA reagent measuring value can be caused due to the unclean cleaning of a reaction cup or a sample adding needle of the biochemical instrument.

In order to eliminate the interference of sodium cholate on TBA detection, the sodium cholate in the interfering reagent kit needs to be removed, but the accuracy of the interfering reagent kit is deteriorated and the detection purpose of the interfering reagent kit cannot be achieved only by removing the sodium cholate. Therefore, in the method for removing the interference on the TBA detection, the phenol substrate in the kit for generating the interference on the TBA detection is replaced by the aniline derivative as the substrate, and the surfactant is added, so that the interference on the TBA detection can be removed, the irritation of the reagent can be reduced, and the injury to a human body can be reduced on the premise of ensuring the accuracy of the reagent.

For the reasons, the invention also provides a kit for removing interference on TBA detection, wherein the R1 reagent of the kit comprises a protective agent, a substrate, a surfactant, an ionic substance and a preservative; wherein the substrate is aniline derivatives.

In specific examples, the substrate is: TOOS, MAOS, TOPS, HDAOS, HALPS or HMMPS.

In specific embodiments, the surfactant is: emullgen A90, Emulgen A60, Emulgen B66, Emullgen 209, Emulgen430, Emullgen 709, polyethylene glycol 2000, polyethylene glycol 4000, polyethylene glycol 6000, Tween-20, Tween-80, polyoxyethylene lauryl ether, glycerol, castor oil, Tritox-405, PVP-40, Triton-114, Triton-100, GENAPOLX-080 or NP-1055.

In specific embodiments, the ionic species comprises one or a combination of two of the following: disodium ethylene diamine tetraacetate, zinc sulfate, sodium chloride, potassium chloride, manganese dichloride, calcium chloride, ammonium chloride, magnesium sulfate, lithium sulfate, ammonium sulfate, magnesium acetate, sodium tetraprocate, potassium carbonate and sodium tartrate.

In specific embodiments, the preservative is PC-300, sodium azide or other preservatives.

TC kit

A TC kit for removing interference with TBA detection, the R1 reagent of the TC reagent comprising PTPES, cholesterol esterase, cholesterol oxidase, peroxidase, protective agent, substrate, surfactant, ionic substance, and preservative; wherein the substrate is aniline derivatives; the R2 reagent of the TC reagent comprises PIPES, 4-aminoantipyrine, sodium azide and protein substances.

Specifically, the replaced TC kit (COD-CE-PAP method) uses the following principle: the cholesterol ester in serum is hydrolyzed into free cholesterol by Cholesterol Esterase (CE), the free cholesterol is oxidized into 4-cholest-3-ketene by Cholesterol Oxidase (COD) to generate hydrogen peroxide, and the generated hydrogen peroxide is catalyzed by oxidase (POD) to generate red quinonimine (Trinder's reaction) by 4-aminoantipyrine (4-AAP) and aniline derivatives (PAP). The absorbance of the sample is directly proportional to the Total Cholesterol (TC) content of the sample, measured colorimetrically at a wavelength of 505 nm. And comparing with the calibrator treated in the same way to calculate the total cholesterol content in the sample.

The R1 reagent of the TC kit includes:

PTPES (20-50 mmol/L); cholesterol esterase (0.1-10 KU/L); cholesterol oxidase (0.1-9 KU/L); peroxidase (3-30 KU/L); all purchased from Nanjing Dolyre Biotechnology, Inc.;

the protective agent is: aspartic acid, glycine, L-serine, oxalic acid, bovine serum albumin or sodium glutamate; are all 0.01 to 1.0 percent by mass; all purchased from Bovine Serum Albumin, Inc.;

the substrates are: TOOS, MAOS, TOPS, HDAOS, HALPS, HMMPS, or other aniline derivatives; the concentration ranges are 0.05-1.8 mmol/L; such as available from Medchemexpress, usa;

the surface active agent is: emullgen A90, Emulgen A60, Emulgen B66, Emullgen 209, Emulgen430, Emullgen 709 (Japan Kao Chemicals); polyethylene glycol 2000, polyethylene glycol 4000, polyethylene glycol 6000, tween-20, and tween-80 (shanghai alading biochem-technological corporation); polyoxyethylene lauryl ether, glycerol, and castor oil (Bailingwei science and technology Co., Ltd.); tritox-405, PVP-40, Triton-114, Triton-100, GENAPOX-080, NP-1055, or other surfactants (Merck Corp.); the concentration ranges are 0.05% -1.0%;

the ionic species includes one or a combination of two of the following: disodium ethylenediaminetetraacetate, zinc sulfate, sodium chloride, potassium manganese dichloride chloride, calcium chloride, ammonium chloride, magnesium sulfate, lithium sulfate, ammonium sulfate, magnesium acetate, sodium tetraprophosphate, potassium carbonate, and sodium tartrate (welfare science and technology, ltd); the concentration of the ionic substance is 2-20 mmol/L.

The preservative is as follows: PC-300 or sodium azide (Beijing Soilebao Tech Co., Ltd.) at a concentration of 1 ml/L.

The R2 reagent of the TC kit includes:

PIPES (20-50mmol/L) (Merck); 4-aminoantipyrine (0.5mmol/L-8mmol/L) (Merck); sodium azide (0.1% by mass) (Merck corporation); proteinaceous substances (concentrations all ranged from 0.05 to 1.00% by mass) (all from the company Bovine Serum Albumin): is bovine serum albumin, glycine, L-serine, oxalic acid, aspartic acid or sodium glutamate.

The following examples and comparative examples of the components of the TC kit are tabulated according to the above description of the TC kit, as in table 1.

TABLE 1

TG kit

A TG kit for relieving interference on TBA detection, wherein R1 reagent of the TG kit comprises HEPES, bile protein lipase, peroxidase, glycerol kinase, phosphoglycerol oxidase, a protective agent, a substrate, a surfactant, ionic substances and a preservative; wherein the substrate is aniline derivatives; the R2 reagent of the TG kit comprises PIPES, 4-aminoantipyrine, sodium azide and protein substances.

Specifically, the principle of use of the TG kit after substitution (GPO-PAP method) was: hydrolyzing TG in serum into glycerol and fatty acid with high-efficiency microbial lipoprotein lipase (LPL), phosphorylating the produced glycerol with Glycerol Kinase (GK) and Adenosine Triphosphate (ATP), oxidizing 3-phosphoglycerol (G-3-P) with phosphoglycerol oxidase (GPO), and developing color with Peroxidase (POD), 4-aminopyrine (4-AAP) and aniline derivatives (PAP). The content of triglyceride can be obtained by detecting the generated quinoneimine under the wavelength of 505 nm.

The reagent R1 of the TG kit includes:

HEPES (high efficiency particulate air): 20-50 mmol/L; lipoprotein lipase (LPL): 18KU/L-40 KU/L; peroxidase (POD): 18KU/L-40 KU/L; glycerol Kinase (GK): 18KU/L-40 KU/L; phosphoglycerol Oxidase (GPO) at a concentration range of 18KU/L to 40 KU/L; all purchased from Nanjing Dolyre Biotechnology, Inc.;

the protective agent is: sucrose, glucose, mannitol, trehalose, lactitol or sorbitol, wherein the concentration ranges from 0.01% to 30% by mass; all from Sigma;

the substrates are: TOOS, MAOS, TOPS, HDAOS, HALPS, HMMPS, or other aniline derivatives; the concentration ranges are all between 0.05 and 1.8mmol/L and are all purchased from Medchem xpress company in the United states;

the surface active agent is: emullgen A90, Emulgen A60, Emulgen B66, Emullgen 209, Emulgen430, Emullgen 709 (Japan Kao Chemicals); polyethylene glycol 2000, polyethylene glycol 4000, polyethylene glycol 6000, tween-20, and tween-80 (shanghai alading biochem-technological corporation); polyoxyethylene lauryl ether, glycerol, and castor oil (Bailingwei science and technology Co., Ltd.); tritox-405, PVP-40, Triton-114, Triton-100, GENAPOX-080, NP-1055, or other surfactants (Merck Corp.);

the ionic species comprises one or a combination of two of the following: disodium ethylenediaminetetraacetate, zinc sulfate, sodium chloride, potassium manganese dichloride chloride, calcium chloride, ammonium chloride, magnesium sulfate, lithium sulfate, ammonium sulfate, magnesium acetate, sodium tetraprophosphate, potassium carbonate, and sodium tartrate (welfare science and technology, ltd); the using concentration is 2-20 mmol/L;

the preservative is as follows: PC-300 or sodium azide (Beijing Soilebao Tech Co., Ltd.) at a concentration of 1 ml/L.

The reagent R2 of the TG kit includes:

HEPES (20-50mmol/L) (Merck); 4-aminoantipyrine (0.5mmol/L-8mmol/L) (Merck); sodium azide (0.1%) (Merck corporation); adenosine Triphosphate (ATP) (concentration range 0.1mmol/L-9mmol/L) (Bovine Serum Albumin Co.); proteinoid (Bovine Serum Albumin Co): bovine serum albumin, glycine, L-serine, oxalic acid, aspartic acid or sodium glutamate, and the concentration range is 0.01-1% by mass percent.

The following examples and comparative examples of the components of the TG kit are tabulated in Table 2, based on the above description of the kit.

TABLE 2

Performance evaluation of TC kit and TG kit, and interference evaluation of TC test or TG test on TBA detection

1. Materials, apparatus and methods

An experimental instrument: HITACHI7180/7100

Experimental parameters:

the method comprises the following steps: temperature of end-point method: 37 deg.C

Dominant wavelength: sub-wavelength of 505 nm: 700nm

Sample size: 3 μ L R1: 240 μ L

R2: reaction direction 60 μ L: forward direction

Reaction time: 10min calibration mode: two point calibration

The interference performance evaluation method comprises the following steps:

the above examples 1 to 20 and comparative examples 1 to 4 were examined as follows:

(1) precision: repeatedly measuring the same sample for 10 times, and calculating CV (%), wherein CV is less than or equal to 3% to meet the requirement;

(2) TC linear range: measuring samples in a range of 0.05-12.90mmol/L, and calculating a correlation coefficient, wherein r is not less than 0.99 and meets the requirement; linear range of TG: and (3) measuring samples in a range of 0.05mmol/L-10.00mmol/L, calculating a correlation coefficient, wherein r is not less than 0.99, and the correlation coefficient meets the requirement.

(3) Accuracy: measuring a third-party quality control product (Landao biochemical quality control product), calculating the deviation between the measured average value and the target value, and calculating the deviation to be less than or equal to 5% to meet the requirement;

(4) stability: the reagent was left at 37 ℃ for 7 days, and the above indices were evaluated: precision, linear range, accuracy;

(5) interference evaluation:

firstly, after cleaning a biochemical analyzer, independently detecting 10 samples with different concentrations by using a TBA kit, and after the detection is finished, cleaning the biochemical analyzer until no interference exists;

detecting 10 samples of the same sample by using a comparison example TC kit (comparison example 1-2) and a TBA kit at the same time, and cleaning a biochemical analyzer until no interference exists after the determination is finished; then, the samples 1 to 11 are respectively detected with the TBA kit at the same time, and the biochemical analyzer is cleaned until no interference exists; thus, the TC kit and the TBA kit were simultaneously detected in 11 groups.

Comparing 11 groups of detection values of the TBA kit and the TC kit which are simultaneously detected with the detection values of the TBA kit which are independently detected, calculating interference deviation, and calculating average interference deviation aiming at 10 samples; the average interference deviation value is less than or equal to 10 percent, and meets the requirement;

the interference evaluation method of the TG kit on the TBA kit is the same as that of TC, and comprises the following steps:

firstly, after cleaning a biochemical analyzer, independently detecting 10 samples with different concentrations by using a TBA kit, and after the detection is finished, cleaning the biochemical analyzer until no interference exists;

simultaneously detecting 10 samples with the same sample by using a TG kit (comparative examples 3-4) and a TBA kit, and cleaning a biochemical analyzer until no interference exists after the detection is finished; then, the samples of examples 12 to 20 were respectively tested with the TBA kit at the same time, and the biochemical analyzer was washed without interference; thus, the TG kit and TBA kit were simultaneously detected for 9 groups in total.

And thirdly, comparing the measured values of the 9 groups simultaneously detected by the TG kit and the TBA kit with the measured values separately detected by the TBA kit, calculating the interference deviation, and calculating the average interference deviation aiming at 10 samples, wherein the average interference deviation value is less than or equal to 10 percent to meet the requirement.

2. Results and conclusions of the experiment

Table 3 and FIG. 1 both show the deviation of the measured values of the untreated TC kit from the TBA kit. The substrates in the TC reagents of comparative examples 1 and 2 were phenol, and sodium cholate was also used in the TC reagent of comparative example 1. In contrast, the reagents of examples 1 to 11 contained no sodium cholate, and the substrates were selected from the aniline derivatives described above. As can be seen from the results in Table 3 and FIG. 1, the mean interference deviation of comparative example 1 is the largest and far exceeds the specified standard range, indicating that the TC kit of comparative example 1 has serious interference on the detection value of the TBA kit. Compared with the mean interference deviation value of the comparative example 1, the TC kit interference deviation values of the examples 1-11 and the comparative example 2 are obviously reduced to reach the specified standard range; this indicates that the TC kits of examples 1 to 11 and comparative example 2 can significantly reduce the variation in the interference with the detection value of the TBA kit without using sodium cholate, and release the effect on the detection of TBA when detecting simultaneously with TBA.

TABLE 3 TC kit to TBA kit assay interference bias results (no heat treatment)

And table 4 and fig. 2 show the precision, linear correlation, accuracy and average interference deviation of the detection value of the TC kit before and after heat treatment for simultaneous detection of the TC kit and the TBA kit. The TC reagent of comparative example 1 severely interfered with TBA detection before and after heat treatment. The TC kit of comparative example 2 removed the interference with TBA detection prior to heat treatment; after the heat treatment, the average interference deviation of the TC kit of the comparative example 2 on the TBA detection is increased and exceeds the range specified by the standard; indicating that the TC kit of comparative example 2 has poor thermal stability. Furthermore, the precision and accuracy of the TC kit of comparative example 2 before and after the heat treatment were higher than those of examples 1 to 11 and comparative example 1, which indicates that the detection of the TC kit itself was greatly affected although the interference with the TBA test could be relieved by merely removing sodium cholate from the TC kit.

Before and after the heat treatment, the accuracy and the average interference deviation of the TC kit of the example 1, 3 and 5-11 are lower than those of the TC kit of the comparative example 1-2 and reach the specified standard range, which indicates that the TC kit of the example 1, 3 and 5-11 has high accuracy and can relieve the interference on the detection of TBA; and after the heat treatment, the average interference deviation and the accuracy change are small, which shows that the TC kit of the examples 1, 3, 5-11 has excellent thermal stability.

In contrast, the TC reagents of examples 2 and 4 all achieved predetermined standards for precision, linear correlation, accuracy and mean interference deviation before heat treatment, and the interference with TBA was removed. On the other hand, since no protein substance was added as a protective agent to the R1 reagent in the TC kits of examples 2 and 4 after the heat treatment, the precision, linear correlation, and accuracy were affected and were out of the specified ranges, which suggests that the addition of a protein protective agent to the R1 reagent in the TC kit can improve the thermal stability.

TABLE 4 summary of TC kit performance and TBA kit detection interference evaluation results

Table 5 and FIG. 3 both show the deviation of the interference of the assay value of the untreated TG kit with that of the TBA kit. The substrates in the TG reagents of comparative examples 3 and 4 were all p-chlorophenol, and sodium cholate was also used in the TG reagent of comparative example 3. In contrast, the reagents of examples 12 to 20 contained no sodium cholate, and the substrates were selected from the aniline derivatives described above. As can be seen from the results of Table 5 and FIG. 3, the mean interference deviation of comparative example 3 is the largest and far exceeds the specified standard range, indicating that the TG kit of comparative example 3 has serious interference on the detection value of the TBA kit. The interference deviation values of the TG kits of examples 12-20 and comparative example 4 were all significantly reduced relative to the interference deviation mean of comparative example 3, to within the specified standard range; this indicates that the TG kits of examples 12 to 20 and comparative example 4 can significantly reduce the variation in the interference with the TBA kit detection value without using sodium cholate, and release the effect on TBA detection when detecting simultaneously with TBA.

TABLE 5 TG kit to TBA kit measurement interference bias results (no heat treatment)

And table 6 and fig. 4 list the precision, linear correlation, accuracy and average interference deviation of the TBA kit detection values before and after heat treatment when TG kit and TBA kit were simultaneously detected. The TG reagent of comparative example 3 severely interfered with TBA detection before and after heat treatment. Prior to heat treatment, the TG kit of comparative example 4 removed the interference with TBA detection; after the heat treatment, the TG kit of comparative example 4 has an increased deviation of the average interference to TBA detection, which is beyond the range specified by the standard; indicating that the TG kit of comparative example 4 is not good in thermal stability. Furthermore, before and after the heat treatment, the precision and accuracy of the TG kit of comparative example 4 were higher than those of examples 12-20 and comparative example 3, which shows that the detection of the TG kit itself was greatly affected although the interference with the TBA test could be relieved by merely removing sodium cholate from the TG kit.

Before and after the heat treatment, the accuracies and the average interference deviations of the TG reagents of the examples 12 and 15-20 are lower than those of the comparative examples 3-4 and reach the specified standard range, which shows that the accuracies of the TG reagents of the examples 12 and 15-20 are high, and the interference on the detection of TBA can be relieved; and after the heat treatment, the average interference deviation and the accuracy change were small, indicating that the TG reagents of examples 12, 15-20 were excellent in thermal stability.

In the TG reagents of examples 13 and 14, the precision, linear correlation, accuracy and mean interference deviation of the TG reagents before heat treatment all reached the specified standards, and the interference with TBA was removed. After the heat treatment, since the R1 reagent in the TG kits of examples 13 and 14 was not added with a saccharide as a protective agent, the precision, linear dependence, and accuracy were affected and exceeded the specified ranges, indicating that the addition of a saccharide protective agent to the R1 reagent in the TG kit improved the thermal stability.

TABLE 6 summary of TG kit performance and TBA kit detection interference evaluation results

In conclusion, the invention provides a method for relieving the interference on TBA detection, sodium cholate in a kit for generating interference on TBA detection is removed, and a phenolic substrate is replaced by an aniline derivative; and a surfactant is added to the kit that interferes with the detection of TBA. The kit for removing the interference on the TBA detection provided by the invention can remove the interference on the TBA detection when the kit and the TBA kit are simultaneously detected on the premise of ensuring the accuracy of the detection. Specifically, a protein protective agent is added into an R1 reagent of the TC kit, or a carbohydrate protective agent is added into an R1 reagent of the TG kit, so that the thermal stability of the TC kit and the thermal stability of the TG kit can be improved.

The above-described embodiments of the present invention should not be construed as limiting the scope of the present invention. Any other corresponding changes and modifications made according to the technical idea of the present invention should be included in the protection scope of the claims of the present invention.