阅读说明：本技术 一种临床样本中微生物核酸保存液 (Microbial nucleic acid preservation solution in clinical sample ) 是由 夏赞贤 陈俊如 凡丽娜 于 2019-03-25 设计创作，主要内容包括：本发明涉及一种临床样本保存液,具体涉及一种临床样本中微生物的DNA核酸和RNA核酸保存液；本发明的临床样本微生物核酸保存液,包括如下组分：质量分数为1%-3%的乙二胺四乙酸二钠、三(羟甲基)氨基甲烷缓冲液,乙醇,并调节PH至6.0-8.0。本发明的临床样本微生物核酸保存液可用于常温下保存临床样本中微生物的DNA和RNA核酸,其操作简单、适用性强、便于运输。(The invention relates to a clinical sample preservative fluid, in particular to a preservative fluid for DNA nucleic acid and RNA nucleic acid of microorganisms in a clinical sample; the invention relates to a microbial nucleic acid preservation solution for clinical samples, which comprises the following components: disodium ethylene diamine tetraacetate with the mass fraction of 1-3%, tris (hydroxymethyl) aminomethane buffer solution and ethanol, and adjusting the PH value to 6.0-8.0. The microbial nucleic acid preservation solution for clinical samples can be used for preserving DNA and RNA nucleic acid of microbes in clinical samples at normal temperature, and is simple to operate, high in applicability and convenient to transport.)

1. A preservation solution for microbial nucleic acid in clinical samples is characterized in that: the buffer solution comprises 1-3% of disodium ethylene diamine tetraacetate, tris (hydroxymethyl) aminomethane buffer solution and ethanol by mass, and the PH value is adjusted to 6.0-8.0.

2. The preservation solution for microbial nucleic acid in clinical specimens according to claim 1, characterized in that: the preservation solution contains disodium ethylene diamine tetraacetate with the mass fraction of 2%.

3. The preservation solution for microbial nucleic acid in clinical specimens according to claim 1, characterized in that: the preservation solution contains a tris (hydroxymethyl) aminomethane buffer solution with the mass fraction of 5%.

4. The preservation solution for microbial nucleic acid in clinical specimens according to claim 1, characterized in that: the preservation solution contains 2% of ethanol by mass fraction.

5. The preservation solution for microbial nucleic acid in clinical specimens according to claim 1, characterized in that: the pH of the preservation solution is adjusted to 7.0.

Technical Field

The invention relates to a clinical sample preservation solution, in particular to a preservation solution for DNA nucleic acid and RNA nucleic acid of microorganisms in a clinical sample.

Background

After the clinical sample is collected, the environment changes, for example, the anaerobic environment changes into the aerobic environment, so that the growth and death speed of bacteria are different from those before collection, and DNA enzyme and RNA enzyme contained in the environment are easy to degrade the microbial nucleic acid in the sample. Therefore, in order to improve the accuracy of detecting the clinical pathogenic microorganisms, the clinical samples need to be preserved, DNA and RNA nucleic acid in the clinical samples are stabilized, and the microorganisms in the clinical samples are fixed in a state just separated from the body as far as possible, so that DNA pathogens and RNA pathogens in the samples can be identified more accurately, and the coverage and the accuracy of detecting the clinical pathogenic microorganisms are improved.

Disclosure of Invention

Problems to be solved by the invention

At present, the preservation of clinical samples mostly adopts a low-temperature freezing mode, for example, the samples are frozen at minus 80 ℃ or minus 20 ℃ immediately after being collected. However, the low-temperature freezing method needs equipment, and in the clinical collection process, the sample cannot be immediately transferred to low-temperature freezing after being collected, and a certain time difference exists, so that the degradation of microbial nucleic acid in the sample is easily caused, the accuracy of the detection of pathogenic microorganisms in the clinical sample is reduced, and the method has use limitation. In order to solve the technical problems, the invention provides a preservation solution which is suitable for preserving microorganism DNA and RNA nucleic acid in a clinical sample at normal temperature, and the preservation solution has the advantages of simple operation, strong applicability and convenient transportation.

Means for solving the problems

The microbial nucleic acid preservation solution in the clinical sample comprises the following components: 1-3% of disodium ethylene diamine tetraacetate, tris (hydroxymethyl) aminomethane buffer solution and ethanol, and adjusting the pH value to 6.0-8.0.

The invention also discloses a preparation process of the preserving fluid, which comprises the following steps:

specifically, the mass fraction of the disodium ethylene diamine tetraacetate in the preservation solution is 2%;

specifically, the mass fraction of the tris (hydroxymethyl) aminomethane buffer solution in the preservation solution is 5%;

specifically, the mass fraction of ethanol in the preservation solution is 2%;

specifically, the pH of the preservation solution is adjusted to 7.0.

ADVANTAGEOUS EFFECTS OF INVENTION

Compared with the prior art, the technical scheme of the invention has the following advantages: the preservation solution can be used for preserving and transporting clinical samples at normal temperature, including cerebrospinal fluid, ascites, sputum, excrement and the like, and the collected samples are immersed in the preservation solution, so that the stability of the composition of microbial DNA and RNA nucleic acid in the samples can be maintained under the normal temperature condition, and a sampler can sample and preserve the samples at any time.

The following describes the embodiments of the present invention in further detail with reference to examples.

Detailed Description

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

It is to be understood that all values and ranges falling between the listed values and ranges of values are intended to be encompassed by the present invention unless otherwise specifically indicated.