阅读说明：本技术 D609在制备抑制细胞铁死亡的药物中的应用 (Application of D609 in preparation of medicine for inhibiting cell iron death ) 是由 韩家淮 倪珩箫 于 2020-07-10 设计创作，主要内容包括：本发明提出了D609在制备抑制细胞铁死亡的药物中的应用,该D609能够有效的抑制脂质过氧化造成的细胞铁死亡,而且其在高于有效浓度作用时完全无副作用,不会造成细胞死亡。(The invention provides an application of D609 in preparing a medicament for inhibiting cell iron death, wherein the D609 can effectively inhibit cell iron death caused by lipid peroxidation, has no side effect at all when the concentration is higher than the effective concentration, and cannot cause cell death.)

Use of D609 in the manufacture of a medicament for inhibiting iron death in a cell.

Use of D609 in the preparation of an inhibitor for lipid peroxidation.

3. The use according to claim 1, wherein D609 is used in an amount of 16.67 μ M.

Technical Field

The invention relates to the technical field of medicinal chemistry, in particular to application of D609 in preparation of a medicament for inhibiting cell iron death.

Background

D609 (tricyclodecane-9-yl-xanthenate) is a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC), can specifically and efficiently inhibit the activity of PC-PLC without influencing the activities of phosphatidylinositol-specific phospholipase C and phospholipase D, has a series of biological functions and activities such as antivirus, anticancer, anti-inflammatory and anti-apoptosis and the like, and thus becomes a good tool for researching the functions of PC-PLC. However, the compounds have been neglected to possess both a lipophilic organic backbone and a thiol-like reducing group. In the existing research reports on D609, D609 is mostly used as a tool for researching PC-PLC, but the inhibition of lipid peroxidation by D609 is not reported.

Disclosure of Invention

In order to solve one of the problems, the invention provides application of D609 in preparing a medicament for inhibiting cell iron death.

To this end, in a first aspect of the invention, the invention proposes the use of D609 in the manufacture of a medicament for inhibiting cellular iron death.

The research shows that the occurrence of cell iron death is finally caused by lipid peroxidation of cell membranes, and the inhibition of lipid peroxidation can effectively block the occurrence of cell iron death. According to the embodiment of the invention, D609 can effectively inhibit cell iron death caused by lipid peroxidation, and has no side effect at all and cannot cause cell death when the concentration is higher than the effective concentration.

In a second aspect of the invention, the invention proposes the use of D609 in the preparation of an inhibitor for lipid peroxidation.

According to the embodiment of the invention, the D609 is used in an amount of 16.67. mu.M.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

FIG. 1 is a graph of cell viability according to example 1 of the present invention;

FIG. 2 is a graph of cell viability according to example 2 of the present invention.

Detailed Description

The technical solution of the present invention is illustrated by specific examples below. It is to be understood that one or more method steps mentioned in the present invention do not exclude the presence of other method steps before or after the combination step or that other method steps may be inserted between the explicitly mentioned steps; it should also be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Moreover, unless otherwise indicated, the numbering of the various method steps is merely a convenient tool for identifying the various method steps, and is not intended to limit the order in which the method steps are arranged or the scope of the invention in which the invention may be practiced, and changes or modifications in the relative relationship may be made without substantially changing the technical content.

In order to better understand the above technical solutions, exemplary embodiments of the present invention are described in more detail below. While exemplary embodiments of the invention have been shown, it should be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

The following reagents and cells were purchased from the market.

The invention will now be described with reference to specific examples, which are intended to be illustrative only and not to be limiting in any way.