阅读说明：本技术 一种无抗高细胞亲和性结肠炎修复剂与应用方法 (Nonreactive high-cell affinity colitis restoration agent and application method thereof ) 是由 王宝维 王茜 葛文华 张名爱 丛红霞 徐慧心 孔敏 凡文磊 于 2020-06-16 设计创作，主要内容包括：本发明提供一种含壳聚糖的甘油二酯制剂,所述的制剂包含有鸭油甘油二酯和壳聚糖。本发明还提供一种溃疡性结肠炎的修复制剂,是使用上述的制剂制备的；其一种具体制备方法,是将鸭油甘油二酯和壳聚糖按一定的体积比充分混合和搅拌制备而成。本发明的修复剂投服前2h,先饲喂4mL/kg干酪乳杆菌(活力单位为10<Sup>8</Sup>CFU/mL)。本发明的组合物对溃疡性结肠炎小鼠具有改善生理指标与肠道菌群结构,降低髓过氧化物酶活性,降低炎症细胞因子的表达,能够保护结肠黏膜层以及隐窝腺体的完整性,能够有效修复溃疡性结肠炎。是一种很有开发价值的新型溃疡性肠道炎症健康修复剂和临床治疗方案。(The invention provides a diglyceride preparation containing chitosan, which comprises duck oil diglyceride and chitosan. The invention also provides a repairing preparation for ulcerative colitis, which is prepared by using the preparation; a specific preparation method is that the duck oil diglyceride and the chitosan are fully mixed and stirred according to a certain volume ratio. 2 hours before the repairing agent is taken, 4mL/kg of lactobacillus casei (the activity unit is 10) is fed firstly 8 CFU/mL). The composition of the invention has the effects of improving physiological indexes and intestinal flora structure and reducing the blood pressure of ulcerative colitis miceLow myeloperoxidase activity, reduced expression of inflammatory cytokines, and can protect the integrity of colon mucosa and crypt gland, and effectively repair ulcerative colitis. Is a novel healthy repairing agent and a clinical treatment scheme for ulcerative intestinal inflammation with great development value.)

1. The ulcerative colitis restoration preparation is characterized by comprising duck oil diglyceride and chitosan solution.

2. The ulcerative colitis restoration formulation of claim 1, wherein the volume ratio of the diglyceride of duck oil to the chitosan solution is 1-2: 1.

3. The ulcerative colitis restoration formulation of claim 1, wherein the volume ratio of the diglyceride of duck oil to the chitosan solution is 1.5: 1.

4. A ulcerative colitis restoration formulation according to any one of claims 1 to 3, wherein the concentration of said chitosan solution is 3%.

5. The method for preparing a ulcerative colitis restoration preparation according to any one of claims 1 to 4, wherein the preparation is completed by adjusting the temperature of the liquid duck oil diglyceride to 25 to 40 ℃, mixing the duck oil diglyceride and the chitosan solution, and stirring for 15 to 20 min.

6. Use of a ulcerative colitis restoration formulation according to any one of claims 1-4 in the preparation of a restoration preparation for ulcerative colitis.

7. The use of claim 6, wherein the repair article further comprises lactobacillus casei.

8. A restorative preparation for ulcerative colitis, comprising the restorative preparation for ulcerative colitis according to any one of claims 1-4 and Lactobacillus casei.

9. The repair kit of claim 8 wherein the lactobacillus casei has a viability of 10⁸CFU/mL。

Technical Field

The invention belongs to the technical field of enteritis treatment and health maintenance preparations, and particularly relates to an antibiotic-free high-cell affinity colitis restoration agent.

Background

Ulcerative colitis is a non-specific colitis whose clinical manifestations include abdominal pain, bloody stool, etc., the lesions are mainly localized to the mucosa and submucosa, and the pathology changes into ulceration, crypt abscess, small vessel inflammation, goblet cell reduction and infiltration of various inflammatory cells. The colitis treatment difficulty lies in that the pathological change part is positioned at the back part of the intestinal tract, and because of the interference of gastric hyperacidity and various enzymes in the intestinal tract, the colitis treatment is easy to degrade when taking antibiotics, the effective concentration is greatly reduced, the colitis is often difficult to cure, and the drug resistance is caused to a certain degree. At present, the clinical treatment of the colitis of human is mainly carried out by sterilization and anti-inflammation, and medicines such as sulfasalazine, mesalazine, nitromer, amoxicillin, enteritis treatment and the like are mostly used; the medicines have side effects and drug resistance of different degrees to people; the medicine can destroy the balance of beneficial bacteria in the body while killing harmful bacteria, and seriously cause the unbalance of intestinal microbial flora; in recent years, the incidence of human beings has been on the rise, with the risk of transformation to colon cancer.

In addition, in the process of raising livestock and poultry, the colitis can be caused due to reasons such as feed mildew, oil rancidity, escherichia coli infection and stress, and huge loss is caused to livestock and poultry breeding, so that the repair treatment of the inflammation of the rear intestinal tract of the livestock and poultry is a technical problem which is generally concerned in livestock and poultry breeding. In the breeding link, the drug residue of commercial livestock and poultry products is easily generated by the administration of antibiotics, so that the food safety problem is caused, and the harm is caused to the health of people. Therefore, the research and development of the efficient preparation and the method for repairing and treating the colitis in a synergistic way have important economic and social significance.

Disclosure of Invention

The invention provides a natural ulcerative colitis restoration preparation, namely a chitosan-containing ulcerative colitis restoration preparation, which uses a composition of duck fat diglyceride (DDG) and Chitosan (CTS) in combination with non-taken lactobacillus casei for restoring body weight, colon length and the like, regulating the expression of inflammatory cytokines and promoting the development of colon intestinal tracts; thereby making up for the deficiencies of the prior art.

The invention firstly provides an ulcerative colitis repairing preparation which comprises duck oil diglyceride and chitosan solution.

The volume ratio of the duck oil diglyceride to the chitosan solution is 1-2: 1; the preferred ratio is 1.5: 1.

The concentration of the chitosan solution is 3 percent;

the preparation method of the repairing preparation comprises the steps of adjusting the temperature of the liquid duck oil diglyceride to be 25-40 ℃, mixing the duck oil diglyceride and the chitosan solution, and stirring for 15-20 min to complete the preparation.

The invention also provides a repair product for ulcerative colitis, which is prepared by using the preparation.

The repair product also comprises lactobacillus casei.

The activity unit of the lactobacillus casei is 10⁸CFU/mL。

The composition can reduce the level of intestinal inflammatory factors, improve the physiological indexes of mice with ulcerative colitis and promote the colon tissue development of the mice with ulcerative colitis. The lactobacillus casei is added into the product to effectively regulate intestinal flora.

Drawings

FIG. 1: h & E staining chart of colon tissue section of duck oil diglyceride and chitosan intervention ulcerative colitis;

FIG. 2: h & E staining pattern of duck oil diglyceride, chitosan and Lactobacillus casei intervening ulcerative colitis colon tissue section.

Detailed Description

Diglyceride is an intermediate product of oil enzymolysis, has wide application in the industries of food, medicine and the like, and has the functions of inhibiting gram-negative bacteria and gram-positive bacteria, stimulating intestinal lipid metabolism and the like. The chitosan is a product of natural polysaccharide chitin with partial acetyl removed, and has the functions of reducing lipogenesis, inhibiting bacterial activity and the like. Amino group-NH in chitosan molecular structure²Will be protonated to form NH in an acidic environment³⁺The ion can be dissolved under the acidic condition, and has a plurality of unique properties such as strong cell affinity, biodegradability and biological effect.

The lactobacillus casei can compete with harmful bacteria for adhesion sites on intestinal epithelial cells in the intestinal tract, so that the adhesive survival of the harmful bacteria on the cells is inhibited; can be adhered and planted on the surface of intestinal mucosa, has regulating and controlling effect on intestinal immune system, and can improve intestinal health, treat intestinal flora disorder and enhance intestinal permeability.

The invention combines different levels of the duck oil diglyceride, the chitosan and the lactobacillus casei for use; the adopted lactobacillus casei can smoothly pass through gastric acid and bile salt to directly enter the intestinal tract, quickly reach the inflammatory surface of the posterior segment of the intestinal tract, quickly establish an intestinal tract dominant probiotic community, and promote the growth of colon tissues and wound healing of mice with ulcerative colitis by combining diglyceride and chitosan. In addition, the lactobacillus casei added and used can promote the growth of beneficial microorganisms in intestinal tracts of the colitis and maintain the structural balance of intestinal flora.

The present invention will be described in detail with reference to examples.