阅读说明：本技术 一种用于病毒消杀的复合酶制剂及其应用 (Complex enzyme preparation for killing viruses and application thereof ) 是由 沈涛 何勇 何语灵 张若燕 王杰鹏 程伟 于 2020-05-27 设计创作，主要内容包括：本发明公开了一种用于病毒消杀的复合酶制剂,包括角蛋白酶,氨肽酶,核酸酶和溶菌酶,还包括酸性蛋白酶、中性蛋白酶和/或碱性蛋白酶；其中,核酸酶和溶菌酶经包囊材料包被；并且公开了复合酶制剂的应用。使用本发明复合酶制剂进行物品、空气、污水、垃圾、粪尿的病毒消杀,消杀效果显著。(The invention discloses a complex enzyme preparation for killing viruses, which comprises keratinase, aminopeptidase, nuclease, lysozyme, acid protease, neutral protease and/or alkaline protease; wherein the nuclease and lysozyme are coated by an encapsulating material; also discloses the application of the compound enzyme preparation. The compound enzyme preparation of the invention is used for killing viruses of articles, air, sewage, garbage and excrement and urine, and has obvious killing effect.)

1. A complex enzyme preparation for killing viruses is characterized in that,

including keratinase, aminopeptidase, nuclease and lysozyme,

also included are acid proteases, neutral proteases and/or alkaline proteases;

wherein the nuclease and the lysozyme are coated with an encapsulating material.

2. A complex enzyme preparation for viral eradication according to claim 1,

the dosage ratio of (acid protease, neutral protease and/or alkaline protease), keratinase, aminopeptidase, nuclease and lysozyme is (10-1000) to (0.1-100) in terms of enzyme activity unit.

3. A complex enzyme preparation for viral eradication according to claim 1,

the dosage ratio of (acid protease, neutral protease and/or alkaline protease), keratinase, aminopeptidase, nuclease and lysozyme is (100-) - (500-) - (20-200): (0.5-20) based on enzyme activity unit.

4. A complex enzyme preparation for viral eradication according to claim 1,

the encapsulating material includes maltodextrin and chitosan.

5. A complex enzyme preparation for viral eradication according to claim 4,

the ratio of the maltodextrin to the chitosan is (2-3) to 1.

6. A complex enzyme preparation for viral eradication according to claim 5,

weighing the materials according to the weight ratio of (nuclease + lysozyme) to maltodextrin to chitosan of (4-5) to (2-3) to 1;

dispersing nuclease into water to prepare a solution A;

dispersing maltodextrin and chitosan in water to prepare solution B;

adding the solution B into the solution A, and uniformly stirring to obtain a solution C;

and (3) spray drying the solution C to obtain the nuclease and lysozyme coated by the encapsulating material.

7. Use of a complex enzyme preparation for viral eradication according to any one of claims 1 to 6 in the eradication of an article or the environment; the environment includes an indoor environment and an outdoor environment.

8. The use according to claim 7,

the application in the disinfection of environmental viruses comprises garbage treatment, excrement treatment, air treatment or sewage treatment.

9. Use of a complex enzyme preparation for viral eradication according to any one of claims 1 to 6 in the preparation of an air filter material.

10. The use of the complex enzyme preparation for virus killing of any one of claims 1-6 in air treatment, characterized in that the complex enzyme preparation is dissolved into enzyme solution, and the virus killing is carried out by passing air through the enzyme solution by using equipment; including but not limited to spray towers.

Technical Field

The invention belongs to the technical field of sanitary protection, and particularly relates to a complex enzyme preparation for killing viruses and application thereof.

Background

There are many ways in which viruses can be transmitted, and those susceptible to infection include contact transmission, droplet transmission, aerosol transmission, fecal oral transmission, and the like. In order to reduce various transmission risks, virus disinfection treatment is generally required for air environment, medical waste, domestic sewage of patients, excrement and urine and the like.

However, the existing disinfectants have poor stability and are easy to decompose, or have strong irritation and corrosiveness, or are not suitable for virus killing treatment of complex component backgrounds, or are easy to cause new environmental pollution, and the application range and the effect are limited.

Therefore, it is desired to provide a product that can be widely applied to various articles and environmental virus killing.

Disclosure of Invention

In view of the above, the invention provides a complex enzyme preparation for virus killing, which can be applied to virus killing treatment of complex component backgrounds such as articles, air, sewage, garbage, excrement and urine, and has good killing effect, safe use and environmental protection.

In order to achieve the purpose, the invention adopts the following technical scheme:

a complex enzyme preparation for killing viruses comprises keratinase, aminopeptidase, nuclease and lysozyme,

also included are acid proteases, neutral proteases and/or alkaline proteases;

wherein, the nuclease and the lysozyme are coated by an encapsulating material.

According to the invention, any one of acid protease, neutral protease and alkaline protease is used together with keratinase, aminopeptidase, nuclease and lysozyme, and the acid protease, the neutral protease or the alkaline protease can be selected according to different acid-base environments, so that the use of the complex enzyme preparation is wider; acid protease, neutral protease and alkaline protease can also be used in combination to make the killing action point more broad-spectrum. Protease firstly effectively decomposes outer membrane protein of the virus to expose nucleic acid thereof; furthermore, nuclease can carry out enzymolysis on nucleic acid into nucleotide, completely destroy the nucleic acid structure of the virus, and achieve the purpose of effectively killing the virus; lysozyme can hydrolyze the sugar chain of viral glycoprotein to destroy the viral protein shell, and can be directly combined with negatively charged viral protein to form double salt with DNA, RNA and coenzyme-removed protein to inactivate virus. The nuclease and the lysozyme are coated, so that the enzyme inactivation caused by improper storage or complex use environment can be avoided; the keratinase, aminopeptidase, acid protease, neutral protease and/or alkaline protease are used as main agents in the complex enzyme preparation and are not coated, so that the efficiency of killing the viruses on articles and the environment can be ensured; in the process of killing, nuclease and lysozyme are gradually released, so that the killing effect is further enhanced.

The complex enzyme preparation used in the invention is tasteless and nontoxic, can adapt to different pH environments, and has good killing effect on various viruses.

Preferably, the dosage ratio of (acid protease, neutral protease and/or alkaline protease), keratinase, aminopeptidase, nuclease and lysozyme is (10-1000) to (0.1-100) in terms of enzyme activity unit.

Preferably, the dosage ratio of (acid protease, neutral protease and/or alkaline protease), keratinase, aminopeptidase, nuclease and lysozyme is (100-.

Preferably, the encapsulating material comprises maltodextrin and chitosan.

Preferably, the ratio of the maltodextrin to the chitosan is (2-3) to 1.

Preferably, the materials are weighed according to the weight ratio of (nuclease + lysozyme): (2-3): 1;

dispersing nuclease into water to prepare a solution A;

dispersing maltodextrin and chitosan in water to prepare solution B;

adding the solution B into the solution A, and uniformly stirring to obtain a solution C;

and (3) spray drying the solution C to obtain the nuclease and lysozyme coated by the encapsulating material.

The complex enzyme preparation for killing viruses is applied to killing of articles and environmental viruses; the environment includes an indoor environment and an outdoor environment.

The application in the environmental virus disinfection comprises garbage treatment, excrement treatment, air treatment or sewage treatment.

Further, the process of killing the environmental viruses needs to use a large dose of complex enzyme preparation, so that the human body needs to be prevented from directly contacting the complex enzyme preparation.

The complex enzyme preparation can be used for preparing air filter materials.

Furthermore, when the complex enzyme preparation is used for preparing the air filtering material, one or more dust filtering layers are arranged on two sides of the complex enzyme preparation, so that the complex enzyme preparation is prevented from escaping along with the filtered air when the complex enzyme preparation is used for a long time.

Further, the application of the complex enzyme preparation in air treatment is to dissolve the complex enzyme preparation into enzyme liquid, and use equipment to enable air to pass through the enzyme liquid for virus killing.

Furthermore, the complex enzyme preparation for killing the viruses can be dissolved into enzyme liquid, the enzyme liquid is sprayed downwards by using a spray tower, and air is convected from bottom to top, so that the viruses in the air are killed.

Furthermore, the complex enzyme preparation can be dissolved into enzyme liquid, the enzyme liquid is placed in a container or equipment, air is introduced into the container or equipment, and the virus killing can be realized through the enzyme liquid by the air.

Further, when the disinfectant is applied to disinfection of articles, the disinfectant can be diluted to a lower concentration properly so as to meet the virus disinfection requirements of articles such as clothes, medical instruments, articles for daily use and the like.

According to the technical scheme, the complex enzyme preparation can be directly used for killing viruses of various articles and environments, is safe and convenient to use, and has an obvious killing effect.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.