阅读说明：本技术 熨斗在定量盘封口中的应用 (Application of iron in quantitative disc sealing ) 是由 谌志强 王景峰 邱志刚 薛斌 李辰宇 王尚 赵辰 张曦 杨晓波 于 2020-04-23 设计创作，主要内容包括：本发明提供了一种熨斗在定量盘封口中的应用,利用温度在140～180℃的熨斗对装有100mL待测水样的定量盘进行封口,可实现对水质微生物指标的检测。本发明突破现有酶底物法中传统封口机的不足,实现了封口机的小型化和便携化,确保酶底物法能在野外、条件较差的实验室等环境下进行水质微生物指标的检测,有效提升了检测人员的水质微生物指标检测工作效率。(The invention provides an application of an iron in sealing a quantitative plate, which is characterized in that the iron with the temperature of 140-180 ℃ is used for sealing the quantitative plate filled with 100mL of water sample to be detected, so that the detection of water quality microorganism indexes can be realized. The invention breaks through the defects of the traditional sealing machine in the existing enzyme substrate method, realizes the miniaturization and the portability of the sealing machine, ensures that the enzyme substrate method can detect the water quality microorganism indexes in the environments such as the field, laboratories with poor conditions and the like, and effectively improves the water quality microorganism index detection work efficiency of detection personnel.)

1. Use of an iron in a dosing tray closure.

2. The use of claim 1, wherein the quantification disc comprises a 51-well or 97-well quantification disc used in an enzyme-substrate assay.

3. Use according to claim 1, characterized in that it comprises the following steps:

1) preheating an iron to a sealing temperature of 140-180 ℃;

2) adding a water sample to be detected into the quantitative disc during the preheating period of the iron, and then flatly placing the quantitative disc on a matched backing plate;

3) after the iron is heated to the sealing temperature, moving the iron from the bottom of the quantitative tray to the opening end of the quantitative tray for sealing;

4) and (4) repeating the operation of the step 3) until no water sample flows out from the open end of the quantitative disc and no liquid flows between the holes, and finishing sealing.

4. Use according to claim 2, wherein the sealing temperature is 150 ℃.

5. Use according to claim 2, wherein the iron is preheated for 2-3 min.

6. The use of claim 2, wherein the sealing time of step 3) is 30-60 s.

7. The use method according to claim 2, wherein the repetition number of the step 4) is 3-5.

8. Use according to claim 2, wherein the dwell time of the iron during the closing process in step 3) is less than or equal to 10s at the same position of the dosing tray.

Technical Field

The invention relates to the technical field of microorganism detection, in particular to application of an iron in quantitative disc sealing.

Background

With the improvement of living standard of people, the security problem of drinking water of people is increasingly emphasized by the nation, and the microbial pollution in water is a key factor for the health of people, especially in rural areas or remote areas, the microbial pollution problem of drinking water is serious. In order to ensure the sanitary and safe drinking water of people, China carries out microbial detection on the drinking water, the detected microbial indexes mainly comprise total coliform, heat-resistant coliform and Escherichia coli, the detection method mainly comprises a multitubular fermentation method, a filter membrane method and an enzyme substrate method, and the enzyme substrate method is favored by a plurality of detection departments due to the advantages of simple and accurate operation, easy learning and use and easy result identification.

The conventional programmable quantitative sealing machine has large volume (about 33 multiplied by 35.5 multiplied by 40.6cm), heavy mass (about 11kg) and high price, which greatly limits the application of the enzyme substrate method in rural areas or fields with poor conditions and the like, so that the working efficiency of the detection of water quality microbiological indexes is low. Therefore, the method needs to find or develop equipment which can replace the traditional sealing machine and has low cost, and ensure that the enzyme substrate method can detect the microbiological indicators of water quality in rural areas, wild fields or laboratories with poor conditions.

Disclosure of Invention

In view of the defects or shortcomings of a sealing machine matched with an enzyme substrate method in the existing water quality microbiology index detection method, the application of the iron in sealing of the quantitative plate is provided.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides an application of an iron in sealing a quantitative disc.

Preferably, the quantitative plate comprises a 51-well or 97-well quantitative detection plate used in an enzyme substrate method detection process.

The above application, preferably, comprises the following steps:

1) preheating an iron to a sealing temperature of 140-180 ℃;

2) adding a water sample to be detected into the quantitative disc during the preheating period of the iron, and then flatly placing the quantitative disc on a matched backing plate;

3) after the iron is heated to the sealing temperature, moving the iron from the bottom of the quantitative tray to the opening end of the quantitative tray for sealing;

4) and (4) repeating the operation of the step 3) until no water sample flows out from the open end of the quantitative disc and no liquid flows between the holes, and finishing sealing.

Preferably, the sealing temperature is 150 ℃.

Preferably, the time for preheating the iron is 2-3 min, and more preferably, the time for preheating the iron is 2.5 min.

Preferably, the sealing time is 30 to 60 seconds.

Preferably, the number of repetitions is 3 to 5.

Preferably, in the sealing process in the step 3), the retention time of the iron is less than or equal to 10s at the same position of the quantitative plate.

Compared with the prior art, the technical scheme of the invention has the following beneficial effects:

(1) after the quantitative plate added with a water sample to be detected is sealed by the iron, the detection results of coliform, total coliform, Escherichia coli and heat-resistant coliform show that compared with the traditional sealing machine, the accuracy of the microorganism index of the detected water quality can be ensured by using the iron as the sealing machine.

(2) Compared with the traditional sealing machine, the iron provided by the invention has the advantages of small volume, light weight, convenience in carrying and low price, ensures that water quality microorganism detection can be smoothly carried out in rural areas, wild areas or laboratories with poor conditions and the like, and effectively improves the water quality microorganism index detection work efficiency of detection personnel.

(3) The iron provided by the invention is applied to sealing the quantitative plate, and when the quantitative plate for sealing the iron is adopted, the situations of liquid leakage and hole breakage do not exist, and the heating time is short.

Detailed Description

The invention provides an application of an iron in sealing a quantitative plate, the iron is not particularly limited, the iron is well known to those skilled in the art, the iron is convenient to carry and easy to seal, and the type of the iron is preferably a foldable iron (purchased from Nippon non-printing good products company, model TPA-MJ 211).

In the present invention, in order to facilitate sealing and reduce sealing time, the width of the iron is preferably greater than or equal to the width of the quantitative plate, and more preferably, the width of the iron is the same as the width of the quantitative plate.

In the invention, the iron is preheated to the sealing temperature of 140-180 ℃.

In the invention, after the temperature adjusting button of the iron is set to the sealing temperature range, the switch of the iron is turned on for preheating.

In the invention, the sealing temperature is too low, which causes the sealing to be not tight, even the sealing effect can not be achieved, thus leading to inaccurate detection result and even incapability of detection work; the sealing temperature is too high, so that the quantitative plate is damaged, a water sample to be detected is overheated, and the accuracy of a detection result is affected, wherein the sealing temperature of the iron is preferably 150-170 ℃, and more preferably 150 ℃.

Compared with a program-controlled quantitative sealing machine, the preheating time of the iron is preferably 2-3 min, more preferably 2.5min, and the preheating time of the program-controlled quantitative sealing machine is 15 min.

During the preheating period of the iron, a water sample to be detected is added into the quantitative disc, and then the quantitative disc is flatly placed on the matched backing plate. In the invention, the water sample to be detected is added in a conventional way, and the quantitative disc is flatly placed on the matched backing plate so as to seal the quantitative disc, so that the sealing is convenient and quick, and the sealed quantitative disc is free from broken holes and liquid leakage.

After the iron is heated to the sealing temperature, the iron is moved from the bottom of the quantitative tray to the opening end of the quantitative tray for sealing. In the present invention, in order to ensure tight sealing between the wells, the iron should be moved from the bottom of the dosing tray to the open end of the dosing tray.

In the invention, when the iron seals the quantitative disc, the residence time of the iron is preferably less than or equal to 10s at the same position of the quantitative disc, if local water samples are heated for too long, the quantitative disc is broken and leaks, and the accuracy of a detection result is further influenced.

In the invention, the step of moving the iron from the bottom of the quantitative tray to the opening end of the quantitative tray for sealing is repeated until no water sample flows out from the opening end of the quantitative tray and no liquid flows between the holes, thus completing the sealing.

In order to ensure that the water sample in the quantitative disc cannot flow out and each hole of the quantitative disc is completely closed, the water sample between each hole is ensured not to circulate any more, and the number of times of the sealing operation is preferably 3-4 times, and more preferably 4 times.

Compared with the traditional sealing machine, the iron of the invention has the advantages that:

traditional cappers such as programmed quantitative capper, size: about 33cm by 35.5cm by 40.6cm, weight: about 11kg, price: 3-5 ten thousand yuan, the flatiron of the invention is as above-mentioned collapsible flatiron, the volume: about 15.2cm × 7cm × 9.8cm (unfolded size), 15.2cm × 7cm × 3.9cm (folded size), weight: 0.4kg, price: 0.03-0.04 ten thousand yuan, so the iron of the invention has small volume, light weight, convenient carrying and low price, and overcomes the limitation of an enzyme substrate method in the environment application of rural areas or fields with poor conditions and the like.

In the invention, no matter the 51-hole quantitative disc or the 97-hole quantitative disc is adopted, the accuracy of the water quality microorganism detection result can be ensured after the sealing is carried out by the iron, and the situations of hole breaking and liquid leakage of the 51-hole quantitative disc or the 97-hole quantitative disc do not occur, so that the sealing machine adopting the iron as the quantitative disc has good sealing effect and can be widely applied to the field of sealing machines.

The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.