阅读说明：本技术 一种用于低抗原蛋白发酵豆粕的酶制剂及应用 (Enzyme preparation for low-antigen protein fermented soybean meal and application ) 是由 刘延杰 赵素珍 陈锋亮 刘培剑 于 2019-12-10 设计创作，主要内容包括：本发明涉及酶技术领域,尤其涉及一种用于低抗原蛋白发酵豆粕的酶制剂及应用。该酶制剂由碱性蛋白酶、中性蛋白酶、植酸酶、半乳糖苷酶和果胶酶组成。该酶制剂与菌剂搭配用于发酵豆粕生产,能够高效降解抗原蛋白等抗营养成分,蛋白消化率高,提升了饲料营养价值,适合于工业化生产。(The invention relates to the technical field of enzymes, and particularly relates to an enzyme preparation for low-antigen protein fermented soybean meal and application thereof. The enzyme preparation consists of alkaline protease, neutral protease, phytase, galactosidase and pectinase. The enzyme preparation is matched with a microbial inoculum for producing fermented soybean meal, can efficiently degrade anti-nutritional ingredients such as antigen protein and the like, has high protein digestibility, improves the nutritional value of the feed, and is suitable for industrial production.)

1. An enzyme preparation for low-antigen protein fermented soybean meal is characterized by consisting of alkaline protease, neutral protease, phytase, galactosidase and pectinase.

2. The enzyme preparation for the low-antigen protein fermented soybean meal as claimed in claim 1, wherein the enzyme activity of the alkaline protease is 30000-60000U/g, the enzyme activity of the neutral protease is 20000-50000U/g, the enzyme activity of the phytase is 1000-3000U/g, the enzyme activity of the galactosidase is 500-2000U/g, and the enzyme activity of the pectinase is 200-1000U/g.

3. The enzyme preparation for the low-antigen protein fermented soybean meal according to claim 1 or 2, which consists of 50000U/g alkaline protease, 50000U/g neutral protease, 2500U/g phytase, 1000U/g galactosidase and 300U/g pectinase.

4. Use of the enzyme preparation according to claim 1 or 2 for the preparation of low-antigen protein fermented soybean meal.

5. The use according to claim 4, wherein the enzyme preparation is used in an amount of 2000g/t fermented soybean meal.

6. The use according to claim 4 or 5, wherein the enzyme preparation is used for preparing low-antigen protein fermented soybean meal in combination with a zymophyte agent.

7. The use of claim 6, wherein the fermentation inoculum is one or more of Lactobacillus plantarum, Lactobacillus acidophilus, Pediococcus acidilactici, Lactobacillus fermentum, Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae.

8. The use according to claim 4, wherein the fermentation period for preparing the low-antigen protein fermented soybean meal is 5-7 days.

Technical Field

The invention relates to the technical field of enzymes, and particularly relates to an enzyme preparation for low-antigen protein fermented soybean meal and application thereof.

Background

Currently, the animal husbandry faces great challenges, the feed digestibility is low, the problems of drug residues of animal products, breeding pollution and the like frequently occur, a series of policy requirements are also put forward by the nation on the banning and the limiting of breeding in the feed, various functional products are developed in a dispute to deal with the existing problems, wherein the fermented feed is known as one of key methods for solving the problems, the fermented soybean meal is most widely applied in the fermented feed, a large number of enterprises are producing the fermented soybean meal, but the quality of each manufacturer is different, and the fermentation level is different. In the prior art, an enzyme preparation is added for producing fermented soybean meal, a fermentation process mainly uses bacteria and the enzyme preparation is used in a synergistic manner, the bacteria mainly use lactic acid bacteria, bacillus and yeast, the enzyme preparation mainly uses protease (neutral protease, acid protease and alkaline protease) as main materials, and non-starch polysaccharidase is used according to specific conditions. The method relieves the problem of unstable quality of fermented soybean meal to a certain extent, can degrade anti-nutritional ingredients such as antigen protein, but has unstable activity of an enzyme preparation, can not ensure batch fermentation level, and is difficult to realize industrial production.

The patent aims to protect the enzyme preparation for fermenting the soybean meal, which has high efficiency degradation on the antigen protein and strong applicability, and research a specific application method of the enzyme preparation to improve the quality of products, degrade anti-nutritional ingredients such as the antigen protein and the like, further improve the nutritional value of the feed, improve the nutritional utilization rate, reduce discharge, alleviate the problems of breeding pollution and the like.

Disclosure of Invention

The enzyme preparation for low-antigen protein fermented soybean meal and the application thereof are scientific and reasonable in combination and collocation, strong in applicability, capable of being combined and matched with various microbial agents and microbial agents in fermented soybean meal production, capable of efficiently degrading antigen protein, improving feed nutritive value and solving the problems in the prior art.

The technical scheme adopted by the invention for solving the technical problems is as follows:

an enzyme preparation for fermenting soybean meal with low antigen protein comprises alkaline protease, neutral protease, phytase, galactosidase and pectinase.

Further, the enzyme activity of the alkaline protease is 30000-60000U/g, the enzyme activity of the neutral protease is 20000-50000U/g, the enzyme activity of the phytase is 1000-3000U/g, the enzyme activity of the galactosidase is 500-2000U/g, and the enzyme activity of the pectinase is 200-1000U/g.

Further, the enzyme preparation consists of 50000U/g of alkaline protease, 50000U/g of neutral protease, 2500U/g of phytase, 1000U/g of galactosidase and 300U/g of pectinase.

The enzyme preparation is applied to the preparation of low-antigen protein fermented soybean meal.

Furthermore, the dosage of the enzyme preparation is 2000g/t of fermented soybean meal.

Further, the enzyme preparation is matched with a zymophyte agent to prepare the low-antigen protein fermented soybean meal.

Further, the fermentation bacteria agent is one or more of lactobacillus plantarum, lactobacillus acidophilus, pediococcus acidilactici, lactobacillus fermentum, bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae.

Furthermore, the fermentation period for preparing the low-antigen protein fermented soybean meal is 5-7 days.

The invention has the beneficial effects that:

the enzyme preparation for the low-antigen protein fermented soybean meal selects specific enzyme types to combine, is used for the production of the low-antigen protein fermented soybean meal through the synergy of bacterial enzymes, can achieve the purpose of efficiently degrading antigen protein, has high protein digestibility and strong applicability to fermentation inoculants, and is suitable for industrial popularization and use.

Detailed Description

In order to clearly illustrate the technical features of the present invention, the present invention is explained in detail by the following embodiments.