阅读说明：本技术 一种过氧化物酶保护剂 (Peroxidase protective agent ) 是由 张海悦 赵青 周玉龙 徐岩 程慧敏 孙景春 *** 于 2019-11-23 设计创作，主要内容包括：本发明公开了一种过氧化物酶保护剂,其配方组成是：磷酸盐缓冲液0.1-0.2mol/L；蔗糖10-30g/；NaCl 2.29-11.69g/L；MgSO<Sub>4</Sub> 1.20-4.81g/L；山梨醇10-30g/L；吐温20为5-20g/L；壳聚糖10-30g/L迭氮钠0.1-1g/L。所述的磷酸盐缓冲液pH＝7.6。本发明可以使过氧化物酶在液体情况下保持良好的长期稳定性。可用于生化检验试剂盒,其酶活性与未添加该保护剂成分的过氧化物酶相比具有更好的稳定性。(The invention discloses a peroxidase protective agent, which comprises the following components in percentage by weight: 0.1-0.2mol/L of phosphate buffer solution; 10-30 g/sucrose; NaCl 2.29-11.69 g/L; MgSO (MgSO) 4 1.20-4.81 g/L; 10-30g/L of sorbitol; tween 20 is 5-20 g/L; 10-30g/L of chitosan and 0.1-1g/L of sodium azide. The pH of the phosphate buffer was 7.6. The invention can keep the peroxidase with good long-term stability under the liquid condition. Can be used in biochemical test kit, and has better stability of enzyme activity compared with peroxidase without the protective agent component.)

1. A peroxidase protective agent characterized by: the formula comprises the following components:

the pH of the phosphate buffer was 7.6.

Technical Field

The invention relates to a peroxidase protective agent, which is applied to the field of in-vitro diagnostic reagent detection.

Background

Peroxidases are a class of oxidases found in a variety of animal, plant and microbial organisms. Catalyzing the oxidation of various reducing agents with hydrogen peroxide. Peroxidase is an enzyme that catalyzes the oxidation of a substrate using hydrogen peroxide as an electron acceptor. The iron porphyrin is mainly present in a peroxisome of a carrier, takes the iron porphyrin as an auxiliary group, can catalyze hydrogen peroxide, oxidize phenolic and amine compounds and hydrocarbon oxidation products to generate colored substances, can be used in the field of in vitro diagnostic reagent detection, and is a common important detection tool enzyme.

The peroxidase is widely applied to a biochemical detection project kit. As a main component of a color development system of the detection kit, the stability of the reagent kit has important influence on the detection of the reagent kit. The stability of the peroxidase directly influences various indexes detected by the biochemical kit. The biochemical kit has good stability, so that the detection result of the biochemical kit in the expiration date is accurate and effective. Peroxidase is a protein in nature and has many factors affecting its activity. Most of the peroxidase currently available on the market is in a dry powder state, and the enzyme activity is not easily lost in the dry powder state. In the biochemical detection reagent, the peroxidase is configured into a solution form, and the peroxidase is easily deformed and inactivated in such a state, so that the whole kit is ineffective.

Disclosure of Invention

In order to solve the above problems, the present invention aims to provide a stable peroxidase protective agent which enables a peroxidase to be stable for a long period of time in a solution system.

A peroxidase protective agent comprises the following components:

the pH of the phosphate buffer was 7.6.

The invention has the beneficial effects that:

the invention can keep the peroxidase with good long-term stability under the liquid condition. Can be used in biochemical test kit, and has better stability of enzyme activity compared with peroxidase without the protective agent component.

Drawings

FIG. 1 is a graph showing a control of the retention of enzyme activity in a control group and three examples of the present invention.

Detailed Description