阅读说明：本技术 一种用于体外诊断的葡萄糖定量检测干片 (Glucose quantitative detection dry tablet for in vitro diagnosis ) 是由 黄利寒 郑日记 甘斌 武伟民 李菁杨 于 2019-10-31 设计创作，主要内容包括：本发明提供一种用于体外诊断的葡萄糖定量检测干片,包括带样品滴加孔的上外壳、扩散层、酶层、色素层、树脂层、支持层、带透光孔的下外壳,其中,上外壳与下外壳相互贴合,上外壳中心位置开有样品滴加孔,下外壳中心位置开有透光孔,扩散层、酶层、色素层、树脂层、支持层通过涂布工艺从上到下依次分层贴合在一起。通过本发明,以解决现有技术存在的荧光光度技术对仪器的要求比较高,在液相中,可以用来检测的分光光度计很贵,且结果不能长期保存；以及胶体金技术则面临产品质量相差较大,不能质控,不能确保质量,且存在后带现象,标本需稀释测试,只能进行定性,不能进行定量的问题。(The invention provides a glucose quantitative detection dry sheet for in vitro diagnosis, which comprises an upper shell with a sample dripping hole, a diffusion layer, an enzyme layer, a pigment layer, a resin layer, a support layer and a lower shell with a light hole, wherein the upper shell and the lower shell are mutually attached, the sample dripping hole is formed in the center of the upper shell, the light hole is formed in the center of the lower shell, and the diffusion layer, the enzyme layer, the pigment layer, the resin layer and the support layer are sequentially attached together in a layered mode from top to bottom through a coating process. The invention solves the problems that the prior fluorescence photometry technology has higher requirements on instruments, a spectrophotometer which can be used for detection is very expensive in a liquid phase, and the result can not be stored for a long time; and the colloidal gold technology has the problems that the product quality is relatively large in difference, the quality cannot be controlled, the quality cannot be ensured, the phenomenon of back zone exists, a sample needs to be diluted and tested, and only qualitative and quantitative measurement can be carried out.)

1. The utility model provides a glucose quantitative determination dry plate for external diagnosis, which is characterized in that, including last shell (1) of taking sample dropwise add hole, diffuse layer (2), enzyme layer (3), pigment layer (4), resin layer (5), supporting layer (6), lower shell (7) of taking the light trap, wherein, go up shell (1) and laminate each other with lower shell (7), it has sample dropwise add hole (8) to go up shell central point to put to open, lower shell central point puts to open has light trap (9), diffuse layer (2), enzyme layer (3), pigment layer (4), resin layer (5), supporting layer (6) are laminated together through coating technology from the top down in proper order.

2. The dry sheet for quantitative glucose measurement for in vitro diagnosis according to claim 1, wherein the diffusion layer (2) mainly comprises hydrophilic polymer material and white reflective material, the wet film thickness of the diffusion layer is 200-400 μm, and the dry film thickness is 60-300 μm.

3. The glucose quantitative determination dry plate for in vitro diagnosis of claim 2, wherein the hydrophilic polymer material is cellulose acetate, and the white reflective material is a mixture of one or more of titanium dioxide, barium sulfate, polystyrene and microcrystalline cellulose.

4. The glucose quantitative determination dry plate for in vitro diagnosis according to claim 1, wherein the enzyme layer (3) comprises glucose oxidase, peroxidase, buffer, and hydrophilic polymer.

5. The dry plate for quantitative measurement of glucose for in vitro diagnosis according to claim 1, wherein the pigment layer (4) comprises a pigment, a buffer, a hydrophilic polymer, the pigment comprising a combination of a hydrogen donor and a coupler.

6. The dry sheet for quantitative determination of glucose for in vitro diagnosis according to claim 5, wherein the hydrogen donor is a 4-aminoantipyrine derivative and the coupler is a 1-hydroxynaphthalene derivative.

7. The dry sheet for quantitative glucose measurement for in vitro diagnosis according to claim 4 or 5, wherein the hydrophilic polymer comprises gelatin, gelatin derivatives, colla Corii Asini, polyvinyl alcohol, polyvinylpyrrolidone or polyacrylamide, and the buffer can be phosphate buffer or citrate buffer.

8. The dry sheet for quantitative measurement of glucose for in vitro diagnosis according to claim 1, wherein the wet film thickness of the pigment layer and the enzyme layer is 50 to 300 μm, and the dry film thickness is 20 to 200 μm.

9. The dry plate for quantitative measurement of glucose for in vitro diagnosis according to claim 1, wherein the support layer (6) is a transparent plastic, and the thickness of the support layer is 30 to 200 μm.

10. The dry sheet for quantitative measurement of glucose for in vitro diagnosis according to claim 1, wherein the resin layer (5) is a water-based epoxy resin, and the wet film thickness of the resin layer is 10 to 100 μm and the dry film thickness is 2 to 50 μm.

Technical Field

The invention relates to an in-vitro diagnosis detection reagent, in particular to a glucose quantitative detection dry tablet for in-vitro diagnosis.

Background

So-called "dry-patch reagents" are relatively conventional "wet chemistry". The method is a mode that liquid in a detected sample is used as a reaction medium, and an object to be detected directly reacts with a reagent fixed on a carrier, and the method is different from the traditional wet chemistry in the medium participating in the chemical reaction. With the development of techniques for separation, purification, storage, etc. of enzymes in biochemistry, the progress of sensor, photometer, and electrode techniques, and the popularization of computers, dry chemistry has also rapidly developed.

Compared with wet chemistry, the dry chemistry analysis method has the advantages of corresponding detection instruments, simple operation, short detection time, accurate obtained result and the like. The application range of a dry chemical test paper method in clinical examination is obviously expanded, more and more biochemical examination items can be carried out, VITORS350 proposed by Olson corporation in America is suitable for a full-automatic analysis system for emergency treatment and conventional biochemistry, samples such as serum, plasma, whole blood, urine, cerebrospinal fluid and the like can be detected, the detection items comprise combined items of liver function, kidney function, myocardial enzyme, blood fat, protein, ions and the like or any single item, the rapid, accurate and accurate result can be really realized, the clinical accurate, rapid and flexible diagnosis requirements can be fully met, but a matched instrument is overlarge, and the detection can not be carried out in real time.

With the progress of biotechnology, the immune dry sheet containing enzyme markers and fluorescence markers, especially colloidal gold or selenium-labeled antibodies (or antigens) developed by utilizing technologies such as immunoosmosis, immunochromatography and the like can be used for analytical determination of troponin, special proteins, hormones, certain therapeutic drugs, virus antibodies or antigens and the like, the multilayer dry sheet adopting a fluorescence photometry and a matched instrument thereof are also available and are used more and more, and a vaginitis quintuplet detection reagent of Henan atlas biology company is used for detecting specific biochemical markers by a dry chemical enzyme method and is used for diagnosis of gynecological diseases. After more than 20 years of development, dry chemistry analysis techniques have been widely used to examine various aspects of medicine, including routine biochemistry, endocrine hormone, toxin drug concentration analysis, and special protein immunoassays. However, the requirement of the fluorescence photometry technology on instruments is high, a spectrophotometer capable of being used for detection is expensive in a liquid phase, and the result cannot be stored for a long time; the colloidal gold technology is used as a primary screening diagnostic reagent for the auxiliary treatment of disease diagnosis because the quality of a product is greatly different, the quality cannot be controlled, the quality cannot be ensured, and the phenomenon of the back zone exists, and a specimen needs to be diluted and tested, can only be qualitative and cannot be quantitative.

Disclosure of Invention

The invention provides a glucose quantitative detection dry sheet for in vitro diagnosis, which aims to solve the problems that the prior art has higher requirements on instruments by a fluorescence photometry technology, a spectrophotometer capable of being used for detection is expensive in a liquid phase, and the result cannot be stored for a long time; and the colloidal gold technology has the problems that the product quality is relatively large in difference, the quality cannot be controlled, the quality cannot be ensured, the phenomenon of back zone exists, a sample needs to be diluted and tested, and only qualitative and quantitative measurement can be carried out.

In order to solve the technical problem, the invention provides a glucose quantitative detection dry sheet for in vitro diagnosis, which comprises an upper shell with a sample dripping hole, a diffusion layer, an enzyme layer, a pigment layer, a resin layer, a support layer and a lower shell with a light hole, wherein the upper shell and the lower shell are mutually attached, the sample dripping hole is formed in the center of the upper shell, the light hole is formed in the center of the lower shell, and the diffusion layer, the enzyme layer, the pigment layer, the resin layer and the support layer are sequentially attached together in a layered manner from top to bottom through a coating process.

The diffusion layer mainly comprises a hydrophilic high polymer material and a white reflecting material, the thickness of the wet film of the diffusion layer is 200-400 mu m, and the thickness of the dry film is 60-300 mu m.

The hydrophilic high polymer material is cellulose acetate, and the white reflective material is a mixture of one or more of titanium dioxide, barium sulfate, polystyrene and microcrystalline cellulose.

The enzyme layer comprises glucose oxidase, peroxidase, a buffer and a hydrophilic polymer.

The pigment layer comprises a pigment, a buffer solution and a hydrophilic polymer, and the pigment comprises a combination of a hydrogen donor and a coupler.

The hydrogen donor is a 4-aminoantipyrine derivative, and the coupler is a 1-hydroxynaphthalene derivative.

The hydrophilic polymer comprises gel, gelatin derivatives, donkey-hide gelatin, polyvinyl alcohol, polyvinylpyrrolidone or polyacrylamide, and the buffer solution is phosphate buffer solution or citric acid buffer solution.

The wet film thickness of the pigment layer and the enzyme layer is 50-300 mu m, and the dry film thickness is 20-200 mu m.

The support layer is made of transparent plastic, and the thickness of the support layer is 30-200 mu m.

The resin layer is water-based epoxy resin, the wet film thickness of the resin layer is 10-100 mu m, and the dry film thickness is 2-50 mu m.

The invention has the following beneficial effects: the invention aims to provide a dry chemical method multi-layer membrane reagent dry sheet for in vitro diagnosis, the method has the advantages of rapid diagnosis, simple operation, cost saving and the like, can improve the accuracy, precision and sensitivity of detection, and has traceability, stable reagent and long storage time.

Drawings

FIG. 1 is a schematic structural diagram of a glucose quantitative determination dry plate for in vitro diagnosis according to an embodiment of the present invention.

Wherein, 1-upper shell, 2-diffusion layer, 3-enzyme layer, 4-pigment layer, 5-resin layer, 6-support layer, 7-lower shell, 8-sample dropping hole and 9-light hole.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

As shown in FIG. 1, the present invention provides a dry sheet for quantitative determination of glucose for in vitro diagnosis, comprising an upper casing 1 with a sample dropping hole, a diffusion layer 2, an enzyme layer 3, a pigment layer 4, a resin layer 5, a support layer 6, and a lower casing 7 with a light transmitting hole. Wherein, the upper shell 1 and the lower shell 7 are mutually jointed. The center of the upper shell 1 is provided with a sample dripping hole 8 for dripping a sample, and the center of the lower shell 7 is provided with a light hole 9. The diffusion layer 2, the enzyme layer 3, the pigment layer 4, the resin layer 5, and the support layer 6 are laminated by a coating process.

The invention consists of a module containing dry chemical reagents attached to a rigid plastic strip. The color of the module changes due to the reaction between various chemical examination contents in the blood and dry chemical reagents, and the color depth or the color change speed of the module is in proportional relation with the concentration of corresponding chemical components in the blood and urine.

Further, the glucose multilayer film dry sheet can be used for quantitative determination of glucose concentration in serum, plasma, urine and cerebrospinal fluid. The glucose dry plate is a multilayer analytical component coated on a polyester substrate, and a sample is dripped to the surface of a diffusion layer through a sample dripping hole of an upper shell and is uniformly distributed to a reagent layer below through the diffusion layer. Under the catalysis of glucose oxidase, glucose in the sample is oxidized to generate hydrogen peroxide and gluconate. The peroxidase then catalyzes the oxidative coupling reaction of the dye precursor to produce a dye. The intensity of the dye is measured by reflected light and is in direct proportion to the concentration of glucose in the blood and urine. The specific reaction is as follows:

wherein, the diffusion layer mainly comprises hydrophilic high molecular material, such as cellulose acetate, and white reflective material, such as titanium dioxide, barium sulfate, polystyrene, microcrystalline cellulose, or mixture thereof; the diffusion layer has the main functions of uniformly diffusing a sample, filtering macromolecular substances and interfering substances in the sample and protecting a reagent layer; the wet film thickness of the diffusion layer is 200-400 μm, and the dry film thickness is 60-300 μm, and the effect is better within 100-250 μm. The reagent layer is a region where a substance to be detected is subjected to a chemical reaction, and different from other inventions, the reagent layer comprises two layers, namely a pigment layer and an enzyme layer, wherein the pigment layer comprises necessary biological agents such as necessary pigment, buffer solution and hydrophilic polymer; the enzyme layer comprises glucose oxidase, peroxidase, buffer solution, hydrophilic polymer and the like; the pigment comprises a combination of a hydrogen donor and a coupler, the hydrogen donor is a 4-aminoantipyrine derivative such as 4-aminoantipyrine, 4-amino-2-methyl-3-phenyl-1- (2, 4, 6-trichlorophenyl) -3-pyrazole-5-1, etc.; the coupler is a 1-hydroxynaphthalene derivative, such as 1, 7-dihydroxynaphthalene, sodium or potassium-hydroxysulfonate, and the like; hydrophilic polymers include gelatin (e.g., acid-washed gelatin, deionized gelatin), gelatin derivatives (e.g., titanates, methyl hydroxymethyl acrylate, etc.), gelatin, polyvinyl alcohol, polyvinyl pyrrolidone, polyacrylamide, etc., with gelatin being preferred. The pH buffer is added to maintain the optimum pH of the oxidase or a pH close to the optimum pH, and a phosphate buffer or a citrate buffer may be used. The wet film thickness of the pigment layer and the enzyme layer is 50 to 300 μm, and the dry film thickness is 20 to 200 μm, and the effect is better within 40 to 200 μm. The support layer can be made of various transparent plastics, such as Polyethylene (PE), polypropylene (PP), Polyester Methyl Methacrylate (PMMA), Polystyrene (PS), Polycarbonate (PC), polyethylene terephthalate (PET), and the like, preferably PET; the thickness of the supporting layer is 30-200 μm, preferably 50-150 μm; the light transmittance of the visible light range is more than 85 percent, the optimal light transmittance is more than 95 percent, and the incident light source and the reflected light can both pass through the supporting layer. The supporting layer and the reagent layer coating film can be better adhered together by means of the resin layer with the adhesion function, so that the requirements on the material and the performance of the base material are reduced, and the cost is saved. The wet film thickness of the resin layer is 10-100 μm, and the dry film thickness is 2-50 μm, and the effect is better within 10-20 μm. Go up the shell layer that shell and lower shell are constituteed and mainly protect reagent in the transportation, store, the deformation that the in-process of test leads to because various power to avoid the damage of reagent, especially in last quick-witted test, no matter be automatic or semi-automatic send into the detection machine with the dry film reagent, the dry film inevitably receives corresponding power, and the reagent shell can avoid the reagent dry film damage. The upper shell and the lower shell can be made of plastic materials.

The preparation method of the dry tablet reagent comprises the following steps:

1. the resin solution (purchased directly) was uniformly coated on a substrate, dried at 45 ℃ for 10min to form a resin layer of uniform thickness.

2. The following pigment layer solutions were further coated on the dried resin layer, dried at 45 ° for 30min, and dried to form a pigment layer having a uniform thickness. The formula of the pigment layer solution is as follows:

3. the following enzyme layer solutions were applied to the dried pigment layer, dried at 45 ℃ for 30min, and dried to form an enzyme layer having a uniform thickness. The formulation of the enzyme layer solution is as follows:

4. and continuously coating the following diffusion layer solution on the dried enzyme layer, drying for 5 hours at the temperature of 25 ℃, and finishing the manufacture of the dry powder dry tablet after drying. The formulation of the diffusion layer solution was as follows:

5. cutting the dry powder sheet into a certain size, and placing the cut dry powder sheet in a dry sheet shell matched with a machine to finish the manufacture of the dry sheet.

The dry chemical reagent prepared by the method has the advantages of excellent performance, stable reagent, long storage time and convenient carrying, and completely realizes the target of immediate detection.

The dry piece detection operation flow is as follows:

1. taking out the dry slices according to the required amount, and returning the temperature for 1 h;

2. the dried tablets were loaded into the test machine and 10ul of whole blood/plasma/urine/cerebrospinal fluid was added drop wise directly to the sample drop on the upper housing of the dried tablet and tested for 5 minutes to yield a result.

And (5) judging a result: referring to the reference value range, the out-of-range is an abnormal value.

The above description is only an example of the present invention, and is not intended to limit the present invention, and it is obvious to those skilled in the art that various modifications and variations can be made in the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.