Liquid feeding device and liquid chromatograph
阅读说明:本技术 送液装置以及液相色谱仪 (Liquid feeding device and liquid chromatograph ) 是由 山口忠行 浅川直树 佐藤友纪 福泽兴祐 村越干昭 于 2017-07-04 设计创作,主要内容包括:送液装置包括:送液泵,经由端部浸渍于收容作为液体的流动相的流动相容器的所述流动相的抽吸用配管,从所述流动相容器中抽吸所述流动相并输送;以及至少一个溶解物质除去过滤器,设于所述流动相的流动路径上的比所述送液泵更靠上游的位置,且填充有填充剂,所述填充剂具有吸附所述流动相中的溶解物质的性质。(The liquid feeding device comprises: a liquid feeding pump that sucks and feeds a mobile phase from a mobile phase container, which contains the mobile phase as a liquid, through a pipe for sucking the mobile phase, the pipe having an end immersed in the mobile phase container; and at least one dissolved substance removal filter which is provided upstream of the liquid sending pump on the flow path of the mobile phase and is filled with a filler having a property of adsorbing the dissolved substance in the mobile phase.)
1. A liquid feeding apparatus comprising:
a liquid feeding pump that sucks and feeds a mobile phase from a mobile phase container, which contains the mobile phase as a liquid, through a pipe for sucking the mobile phase, the pipe having an end immersed in the mobile phase container; and
and at least one dissolved substance removal filter which is provided upstream of the liquid sending pump on the flow path of the mobile phase and is filled with a filler having a property of adsorbing the dissolved substance in the mobile phase.
2. The liquid feeding apparatus according to claim 1, wherein the dissolved substance removing filter includes a dissolved substance removing filter in which the filler is filled in a suction filter provided at the end of the suction pipe.
3. The liquid feeding apparatus according to claim 1 or 2, wherein a plurality of the dissolved substance removal filters are provided in series along a flow path of the mobile phase.
4. The liquid feeding apparatus according to claim 3, wherein the plurality of dissolved substance removal filters are dissolved substance removal filters filled with a filler having a property of adsorbing dissolved substances different from each other.
5. The liquid conveying apparatus according to any one of claims 1 to 4, wherein the filler is any one of activated carbon, silica gel, a polymer, silica gel whose surface is modified with a functional group, or a polymer whose surface is modified with a functional group.
6. A liquid chromatograph comprising:
analyzing the flow path;
the liquid feeding apparatus according to any one of claims 1 to 5, wherein a mobile phase is fed in the analysis flow path;
a sample injection unit configured to inject a sample into the analysis channel;
an analysis column provided downstream of the sample injection section in the analysis channel and configured to separate a sample injected into the analysis channel by the sample injection section for each component; and
and a detector provided downstream of the analytical column in the analytical flow path, for detecting the sample component separated by the analytical column.
Technical Field
The present invention relates to a liquid feeding device for feeding a mobile phase as a liquid, and a liquid chromatograph (liquid chromatograph) using the liquid feeding device.
Background
An analysis device such as a liquid chromatograph is provided with a liquid feeder that feeds a liquid as a mobile phase. The liquid feeding device immerses an end of a suction pipe in the mobile phase contained in the mobile phase container, and sucks and feeds the mobile phase by a liquid feeding pump through the suction pipe.
A filter called a suction filter (see patent document 1) is generally attached to an end of a pipe for sucking the mobile phase immersed in the mobile phase container. By providing a suction filter at an end of the suction pipe, it is possible to remove solid substances in the mobile phase while sucking the mobile phase from the mobile phase container.
[ Prior art documents ]
[ patent document ]
Patent document 1: japanese patent laid-open No. 2006-038738
Disclosure of Invention
[ problems to be solved by the invention ]
A liquid used as a mobile phase in an analysis apparatus such as a liquid chromatograph may be contaminated in all places such as the use environment of the apparatus (a laboratory, a pretreatment substance, and the like), an ultrapure water purification apparatus, and a mobile phase container. For example, carbon dioxide in the intake air dissolves in the liquid contained in the mobile phase container, or the residue of a cleaning agent for cleaning the mobile phase container dissolves in the liquid that becomes the mobile phase, and the mobile phase is contaminated.
In recent years, liquid chromatography is typified by mass spectrometry and has improved detector sensitivity, and compounds at lower concentrations can be analyzed. However, in the high sensitivity analysis, even if the mobile phase is slightly contaminated as described above, substances other than the analysis target substance dissolved in the mobile phase may be detected by the detector, which may hinder the analysis. Further, the mobile phase, which is only slightly contaminated, enters the analysis system, and a contaminant may adhere to the inside of the pipe or the column to contaminate the analysis system itself, which may cause a reduction in detection sensitivity.
However, conventionally used suction filters cannot remove contaminants dissolved in a mobile phase. On the other hand, if a member for removing such contaminants is provided in the analysis system, the dead volume (dead volume) may become large and the separation capacity may be reduced. In particular, when gradient analysis (gradient analysis) is performed in which a sample is separated and analyzed while changing the composition of a mobile phase in a liquid chromatograph, if the internal capacity in the system downstream of a liquid sending pump is large, the change in the composition of the mobile phase is also slowed down by that amount, and the analysis efficiency is lowered.
Therefore, an object of the present invention is to remove contaminants dissolved in a mobile phase without increasing the dead volume in the system.
[ means for solving problems ]
The liquid feeding device of the present invention includes: a liquid feeding pump that sucks and feeds a mobile phase from a mobile phase container, which contains the mobile phase as a liquid, through a pipe for sucking the mobile phase, the pipe having an end immersed in the mobile phase container; and at least one dissolved substance removal filter which is provided upstream of the liquid sending pump on the flow path of the mobile phase and is filled with a filler having a property of adsorbing the dissolved substance in the mobile phase.
In the liquid feeding device according to the present invention, it is preferable that the dissolved substance removing filter includes a dissolved substance removing filter in which the filler is filled in a suction filter provided at the end of the suction pipe. In general, since a suction filter is provided at an end of a pipe for sucking the mobile phase immersed in the mobile phase container, an increase in the internal capacity of the system between the mobile phase container and the liquid sending pump can be suppressed by filling the filler in the suction filter to form a dissolved substance removing filter.
In addition, there may be a case where the contaminant dissolved in the mobile phase cannot be completely removed by only one dissolved substance removal filter. In this case, the plurality of dissolved substance removal filters may be provided in series along the flow path of the mobile phase.
In this case, the plurality of dissolved substance removing filters provided in series are preferably dissolved substance removing filters filled with a filler having a property of adsorbing dissolved substances different from each other. In this way, even when a plurality of contaminants are dissolved in the mobile phase, these contaminants can be removed from the mobile phase.
The filler to be filled in the dissolved substance removing filter includes: activated carbon, silica gel, polymers, silica gel with a surface modified with functional groups, polymers with a surface modified with functional groups, and the like.
The liquid chromatograph of the present invention comprises: analyzing the flow path; the liquid feeding device described above that feeds a mobile phase in the analysis flow path; a sample injection unit configured to inject a sample into the analysis channel; an analysis column provided downstream of the sample injection section in the analysis channel and configured to separate a sample injected into the analysis channel by the sample injection section for each component; and a detector provided downstream of the analytical column in the analytical flow path, for detecting the sample component separated by the analytical column.
[ Effect of the invention ]
The liquid feeding device of the present invention includes at least one dissolved substance removal filter filled with a filler having a property of adsorbing dissolved substances in a mobile phase, and therefore can remove contaminants dissolved in the mobile phase. Further, the dissolved substance removing filter does not increase the system volume of the analyzer on the downstream side of the liquid feeding pump even at the upstream side of the liquid feeding pump in the flow path of the mobile phase.
The liquid chromatograph of the present invention is provided with the liquid feeding device, and therefore, the detection of substances other than the substance to be analyzed in the highly sensitive analysis is suppressed by removing the contaminating substance in the mobile phase. In addition, the mobile phase from which the contaminant is removed flows in the analysis flow path, and therefore contamination in the analysis system is prevented.
Drawings
Fig. 1 is a flow path configuration diagram schematically showing an embodiment of a liquid chromatograph.
FIG. 2 is a sectional view showing an example of the dissolved substance removing filter of the liquid feeding apparatus according to the embodiment.
Fig. 3 is a sectional view showing another example of the dissolved substance removing filter.
Fig. 4 is a cross-sectional view showing another example of the dissolved substance removal filter.
Detailed Description
Hereinafter, an embodiment of the present invention will be described with reference to the drawings.
First, an embodiment of a liquid chromatograph will be described with reference to fig. 1.
The liquid chromatograph of the present embodiment includes a
The
The liquid-
The structure of the dissolved
The dissolved
The internal space of the
An
The dissolved
However, when the contaminants dissolved in the
The structure of the dissolved
The dissolved
A
An
In the example of fig. 3, two dissolved
In the above-described embodiment, the dissolved
In the above-described embodiment, the liquid chromatograph has been described as an example of the analysis device provided with the
[ description of symbols ]
1: liquid feeding device
2a, 2 b: liquid feeding pump
4a, 4a', 4 b: suction pipe
6a, 6a', 6 b: dissolved substance removing filter
8a, 8 b: flow compatilizer
10: mixing device
12: analytical flow path
14: sample injection part
16: analytical column
18: detector
20. 32: body part
22. 36: cover part
24. 38: protrusion
26. 34, 40: connecting hole
28. 42: filler
30. 43, 44: filter
46: and (4) sucking a filter.
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