阅读说明：本技术 一种基于旋转液腔的生物气溶胶收集方法 (Biological aerosol collecting method based on rotary liquid cavity ) 是由 付钰 许添顺 卢维来 王璐 李焓菲 于 2019-09-16 设计创作，主要内容包括：本发明提供了一种基于旋转液腔的生物气溶胶收集方法,其中,所述生物气溶胶收集方法包括以下步骤：(1)向收集瓶中加入收集液,将所述收集瓶放置在磁力搅拌器上并通过所述磁力搅拌器使所述收集液形成旋转液腔；其中,所述收集瓶包括瓶体和位于所述瓶体内的空心柱,所述空心柱设有伸出所述瓶体的收集瓶进口,所述瓶体的上部侧壁设有收集瓶出口,所述空心柱具有分散柱头,所述分散柱头位于所述瓶体的下部空间,所述分散柱头上设有气孔；以及其中,所述分散柱头位于所述旋转液腔的内部空间中；和(2)采用与所述收集瓶出口连通的真空泵将生物气溶胶吸入到所述收集瓶中进行收集。(The invention provides a bioaerosol collection method based on a rotary liquid cavity, wherein the bioaerosol collection method comprises the following steps of: (1) adding a collection liquid into a collection bottle, placing the collection bottle on a magnetic stirrer, and enabling the collection liquid to form a rotary liquid cavity through the magnetic stirrer; the collecting bottle comprises a bottle body and a hollow column positioned in the bottle body, the hollow column is provided with a collecting bottle inlet extending out of the bottle body, the side wall of the upper part of the bottle body is provided with a collecting bottle outlet, the hollow column is provided with a dispersing column head, the dispersing column head is positioned in the lower space of the bottle body, and the dispersing column head is provided with an air hole; and wherein the dispersion column head is located in the interior space of the rotating liquid chamber; and (2) sucking the bioaerosol into the collecting bottle for collection by adopting a vacuum pump communicated with the outlet of the collecting bottle.)

1. A bioaerosol collection method based on a rotating liquid chamber, wherein the bioaerosol collection method comprises the following steps:

(1) adding a collection liquid into a collection bottle, placing the collection bottle on a magnetic stirrer, and enabling the collection liquid to form a rotary liquid cavity through the magnetic stirrer; the collecting bottle comprises a bottle body and a hollow column positioned in the bottle body, the hollow column is provided with a collecting bottle inlet extending out of the bottle body, the side wall of the upper part of the bottle body is provided with a collecting bottle outlet, the hollow column is provided with a dispersing column head, the dispersing column head is positioned in the lower space of the bottle body, and the dispersing column head is provided with an air hole; and wherein the dispersion column head is located in the interior space of the rotating liquid chamber; and

(2) and sucking the bioaerosol into the collecting bottle for collection by adopting a vacuum pump communicated with the outlet of the collecting bottle.

2. The bioaerosol collection method of claim 1, wherein the collection fluid is PBS buffer.

3. The bioaerosol collection method of claim 1 or 2, wherein the dispersion stigma has three evenly distributed nozzles.

4. The bioaerosol collection method of any one of claims 1 to 3, wherein the inside diameter of the vial is 20 to 100mm, preferably 35 mm.

5. The bioaerosol collection method of any one of claims 1 to 4, wherein the collection fluid added in step (1) is 0.1 to 0.2 times, preferably 0.15 times the volume of the vial.

6. The bioaerosol collection method of any one of claims 1 to 5, wherein the gas orifices of the dispersion stigma in step (1) are tangential to the internal liquid surface of the rotating liquid chamber.

7. The bioaerosol collection method of any one of claims 1 to 6, wherein the magnetic stirrer in step (1) is rotated at a speed of 100 to 1000 rpm, preferably 760 rpm.

8. The bioaerosol collection method of any one of claims 1 to 7, wherein a gas flow meter is provided between the collection bottle outlet and the vacuum pump.

9. The bioaerosol collection method of claim 8, wherein the collector bottle outlet is connected to the gas flow meter and the gas flow meter is connected to the vacuum pump by rubber hoses.

10. The bioaerosol collection method of claim 8 or 9, wherein the vacuum pump is provided with a control knob, the bioaerosol collection method further comprising the steps of: controlling the gas flow rate through a control knob and a gas flowmeter;

preferably, the gas flow rate is 10L/min.

Technical Field

The invention relates to a biological aerosol collecting method based on a rotary liquid cavity.

Background

The sampling of the bioaerosol is one of the key steps for accurately researching the microorganisms carried by the aerosol, and the basic principle of the accurate sampling of the bioaerosol is to efficiently and completely collect all the microorganisms. Generally, the principle of sampling biological aerosol and non-biological aerosol is basically the same, and gravity settling, inertial impaction, filtration, electric or thermal precipitation and other methods are adopted.

However, unlike non-bioaerosols, bioaerosols are targeted to microorganisms that are biologically active, and therefore it is necessary to consider whether various technical parameters influence the activity and culturability of the cells during sampling. Currently, commercial Anderson impactors, SKC biosampler,Mu, cyclone sampler and other bioaerosol collecting devices have larger flow velocity, have certain influence on the activity of the biological particles in the bioaerosol, and part of the bioaerosol particles are rebounded no matter liquid impact or solid impact, so that part of the bioaerosol is lost. In addition, these devices have the disadvantages of high cost, complex and heavy structure, and inconvenient operation.

Disclosure of Invention

Therefore, the present invention aims at providing a bioaerosol collecting method based on a rotary liquid cavity, which has high efficiency and simple operation.

The purpose of the invention is realized by the following technical scheme.

The invention provides a bioaerosol collection method based on a rotary liquid cavity, wherein the bioaerosol collection method comprises the following steps of:

(1) adding a collection liquid into a collection bottle, placing the collection bottle on a magnetic stirrer, and enabling the collection liquid to form a rotary liquid cavity through the magnetic stirrer; the collecting bottle comprises a bottle body and a hollow column positioned in the bottle body, the hollow column is provided with a collecting bottle inlet extending out of the bottle body, the side wall of the upper part of the bottle body is provided with a collecting bottle outlet, the hollow column is provided with a dispersing column head, the dispersing column head is positioned in the lower space of the bottle body, and the dispersing column head is provided with an air hole; and wherein the dispersion column head is located in the interior space of the rotating liquid chamber; and

(2) and sucking the bioaerosol into the collecting bottle for collection by adopting a vacuum pump communicated with the outlet of the collecting bottle.

Preferably, the collection solution is PBS buffer.

Preferably, the dispersion mast head has three evenly distributed nozzles.

Preferably, the inner diameter of the bottle body is 20-100 mm, and preferably 35 mm.

Preferably, the side wall of the bottle body is provided with scales.

Preferably, the collecting liquid added in the step (1) is 0.1-0.2 times of the volume of the bottle body, and preferably 0.15 times.

Preferably, the air holes of the dispersion column head in the step (1) are tangent to the inner liquid surface of the rotary liquid cavity.

Preferably, the rotation speed of the magnetic stirrer in the step (1) is 100-1000 rpm, and is preferably 760 rpm.

Preferably, a gas flow meter is arranged between the outlet of the collecting bottle and the vacuum pump.

Preferably, the outlet of the collecting bottle is connected with the gas flowmeter through rubber tubes, and the gas flowmeter is connected with the vacuum pump through rubber tubes.

Preferably, a control knob is arranged on the vacuum pump, and the bioaerosol collecting method further comprises the following steps: the gas flow rate is controlled by a control knob and a gas flowmeter.

More preferably, the gas flow rate is 10L/min.

The invention has the following advantages:

(1) the bioaerosol collecting method can collect bioaerosol conveniently and efficiently in most environments.

(2) According to the invention, the rotary liquid cavity is formed in the collecting bottle through the magnetic stirrer, the bioaerosol particles are pumped into the collecting bottle through the hollow column and are uniformly dispersed and beaten on the liquid cavity by the rotary water power in a soft landing mode through the dispersion column cap, the damage to microorganisms is low, and the liquid cavity is rotary, so that the phenomenon of rebounding when the bioaerosol particles impact on the liquid surface is avoided to a great extent, the activity of the microorganisms is further favorably maintained, and the collecting efficiency of the bioaerosol is improved.

(3) The bioaerosol collection method is light, simple and low in cost, and is favorable for popularization and application.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic view of one embodiment of a bioaerosol collection process of the present invention;

FIG. 2 is a schematic diagram of the formation of a rotating fluid chamber;

FIG. 3 is a schematic view of one embodiment of a collection bottle;

FIG. 4 is a graph comparing the fungal collection efficiency for tests I-IV, where test I used a collection bottle with a five-hole truncated cone-shaped dispersion head, without a rotating liquid chamber; in the test II, a collecting bottle with a three-hole truncated cone-shaped dispersing column head is adopted, and a rotary liquid cavity is not formed; experiment III adopts a collecting bottle with three-hole truncated cone-shaped dispersing column heads to form a rotary liquid cavity; experiment IV adopts a collecting bottle provided with 3 dispersing column heads with uniformly distributed nozzles to form a rotary liquid cavity;

FIG. 5 is a graph comparing the efficiency of collection of E.coli bacterial aerosol nebulization;

FIG. 6 is a graph comparing mortality of a nebulized aerosol of collected E.coli bacteria; and

FIG. 7 is a graph comparing the efficiency of E.coli bacterial aerosol collection with different volumes of collection fluid.

Wherein the figures include the following reference numerals:

1-inlet of collecting bottle, 2-outlet of collecting bottle, 3-dispersing column cap, 4-scale, 5-magnetic stirrer, 6-rubber tube, 7-gas flowmeter, 8-vacuum pump, 9-control knob, 10-bottle body, 11-hollow column, 12-air hole, 13-rotary liquid cavity, 14-rotor, 15-collecting bottle, 16-internal space of rotary liquid cavity, and 17-nozzle.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.

The invention provides a bioaerosol collection method based on a rotary liquid cavity, wherein the bioaerosol collection method comprises the following steps of:

(1) adding a collection liquid into a collection bottle, placing the collection bottle on a magnetic stirrer, and enabling the collection liquid to form a rotary liquid cavity through the magnetic stirrer; the collecting bottle comprises a bottle body and a hollow column positioned in the bottle body, the hollow column is provided with a collecting bottle inlet extending out of the bottle body, the side wall of the upper part of the bottle body is provided with a collecting bottle outlet, the hollow column is provided with a dispersing column head, the dispersing column head is positioned in the lower space of the bottle body, and the dispersing column head is provided with an air hole; and wherein the dispersion column head is located in the interior space of the rotating liquid chamber; and

(2) and sucking the bioaerosol into the collecting bottle for collection by adopting a vacuum pump communicated with the outlet of the collecting bottle.

Fig. 1 shows one embodiment of the bioaerosol collection method of the present invention, and fig. 2 shows the formation of a rotating liquid chamber. As shown in fig. 1 and 2, the collection bottle 15 includes a bottle body 10 and a hollow post 11 positioned within the bottle body 10. The hollow column 11 is provided with a collecting bottle inlet 1 extending out of the bottle body 10, the upper part of the side wall of the bottle body 10 is provided with a collecting bottle outlet 2, the dispersing column head 3 of the hollow column 11 is provided with air holes 12, and the dispersing column head 3 is positioned in the lower space of the bottle body 10.

Referring to fig. 1 and 2, the bioaerosol collection method of the present invention comprises the steps of:

(1) adding the collected liquid to the collection bottle 15, placing the collection bottle 15 on the magnetic stirrer 5 and rotating the collected liquid by the magnetic stirrer 5, thereby forming a rotating liquid chamber 13 in the lower part of the collection bottle 15, the dispersion column head 3 being located in the inner space 16 of the rotating liquid chamber; and

(2) the bioaerosol is sucked into the collection bottle 15 for collection by a vacuum pump 8 which is in communication with the collection bottle outlet 2.

According to the invention, the rotary liquid cavity is formed in the collecting bottle through the magnetic stirrer, the bioaerosol particles are pumped into the collecting bottle through the hollow column and are uniformly dispersed and beaten on the liquid cavity by the rotary water power in a soft landing mode through the dispersion column cap, so that the damage to microorganisms is low, and the liquid cavity is rotary, so that the occurrence of rebound when the bioaerosol particles impact on the liquid surface is avoided to a great extent, the maintenance of the activity of the microorganisms is facilitated, and the collecting efficiency of the bioaerosol is improved.

According to an embodiment of the invention, the collection liquid is a liquid capable of collecting, loading microorganisms.

In the present invention, a suitable collection liquid may be selected depending on the type of microorganisms in the bioaerosol. In some preferred embodiments, the collection fluid is water, PBS buffer, or liquid nutrient.

According to one embodiment of the invention, with reference to fig. 3, the dispersion mast head 3 has 3 nozzles 17 distributed uniformly.

According to an embodiment of the present invention, the inner diameter of the bottle body 10 is 20 to 100mm, preferably 35 mm.

According to the preferred embodiment of the present invention, wherein the inner diameter of the bottle body 10 is 35mm, the inner diameter of the hollow column 11 is 8mm, the dispersion column head 3 has 3 nozzles 17 uniformly distributed on the circumference having a diameter of 13mm, the inner diameter of the nozzles 17 (i.e., the diameter of the air holes 12) is 3mm, and the distance from the nozzles 17 to the bottom of the bottle body 10 is 20 mm.

According to an embodiment of the present invention, the bottle body 10 is provided with a scale 4 on a sidewall thereof.

According to an embodiment of the present invention, the amount of the collection liquid added in step (1) is 0.1 to 0.2 times, preferably 0.15 times the volume of the bottle.

According to an embodiment of the invention, wherein the higher the rotational speed of the magnetic stirrer (its rotor), the larger the inner space of the rotating liquid chamber is formed, which results in the air holes on the dispersion head being far from the inner liquid surface of the rotating liquid chamber, which in turn results in a reduced collection efficiency, which is highest when the air holes on the dispersion head are tangential to the inner liquid surface of the rotating liquid chamber, and which has a minimal effect on the microbial activity.

In some preferred embodiments, the air holes 12 of the dispersion column head 3 in the step (1) are tangential to the inner liquid surface of the rotating liquid cavity 13.

According to one embodiment of the present invention, in step (1), the rotation speed of the magnetic stirrer 5 is 100 to 1000 rpm, preferably 760 rpm.

In some embodiments, the rotor 14 is disposed in the bottle 10, and the magnetic stirrer 5 rotates the rotor 14 to form the rotating liquid chamber 13.

According to one embodiment of the invention, a gas flow meter 7 is provided between the collector bottle outlet 2 and the vacuum pump 8.

In the invention, the flow rate of the gas sucked into the collecting bottle 15 can be detected and checked at any time through the gas flowmeter 7.

According to one embodiment of the invention, the connection between the collector flask outlet 2 and the gas flow meter 7 and between the gas flow meter 7 and the vacuum pump 8 is by means of hoses 6.

According to an embodiment of the present invention, wherein the vacuum pump 8 is provided with a control knob 9, the bioaerosol collecting method further comprises the following steps: the gas flow rate is controlled by a control knob 9 and a gas flow meter 8.

At present, the air flow velocity in the commercialized bioaerosol collection process is usually more than 10L/min, especially the Anderson impactor,Mu and the sampling flow rate of the cyclone sampler are far more than 10L/min. Only the sampling flow rates of SKC biosampler and AGI-30 were at 12.5L/min, but they were too costly. When the biological sample in the air is collected, a cutting process is carried out in the collecting bottle, and the activity of the microorganism in the biological aerosol is better when the gas flow rate is low.

According to one embodiment of the invention, wherein the invention does not require high gas flow rates, microbial activity can be maintained. In some embodiments, the gas flow rate is 10L/min.