阅读说明：本技术 将衔接子附接至样品核酸的方法 (Method of attaching adapters to sample nucleic acids ) 是由 安德鲁·肯尼迪 斯特凡尼·安·沃德·莫蒂默 于 2018-04-13 设计创作，主要内容包括：公开了制备具有单链突出端的双链核酸以便扩增和测序的方法。使平端双链核酸分子与Taq接触导致单核苷酸向核酸的3’末端的非模板指导的添加,其中A被最频繁地添加A,随后是G,随后是C和T。G加尾频繁得足以使得核酸分子与衔接子的连接效率可以通过包括用T和C加尾的衔接子而显著增加。用平端衔接子连接未成功进行加尾的平端核酸分子,连接效率可以甚至进一步增加。(Methods of preparing double-stranded nucleic acids with single-stranded overhangs for amplification and sequencing are disclosed. Contacting the blunt-ended double-stranded nucleic acid molecule with Taq results in non-template directed addition of single nucleotides to the 3' end of the nucleic acid, where a is added most frequently with a, followed by G, followed by C and T. G-tailing is frequent enough that the ligation efficiency of nucleic acid molecules to adapters can be significantly increased by including adapters that are tailed with T and C. Ligation of blunt-ended nucleic acid molecules that have not been successfully tailed with blunt-ended adaptors can increase the efficiency of ligation even further.)