阅读说明：本技术 一种食品病菌检测试剂 (Food pathogen detection reagent ) 是由 杨静 龙刚 王欢 李博 于 2021-06-17 设计创作，主要内容包括：本发明公开了一种食品病菌检测试剂,包括培养试剂和盛放器皿,所述培养试剂贮存于盛放器皿中；所述培养试剂组分按照重量份数为：牛肉膏粉50-100份、蛋白胨150-300份、氯化钠50-100份、葡萄糖100-200份、琼脂150-300份、卡那霉素10-30份,所述培养试剂按照重量份数称取,通过反应管摇晃混合,PH值控制在6.8-7.5,混合后的所述培养试剂通过处理后倾注到所述盛放器皿中。本发明可抑制参杂的其他病菌生长,既保证了肉毒梭菌的正常生长,又避免了其他参杂的其他病菌扰乱工作人员对肉毒梭菌的观察、分析视野。(The invention discloses a food pathogen detection reagent, which comprises a culture reagent and a containing vessel, wherein the culture reagent is stored in the containing vessel; the culture reagent comprises the following components in parts by weight: 50-100 parts of beef extract powder, 150-300 parts of peptone, 50-100 parts of sodium chloride, 100-200 parts of glucose, 150-300 parts of agar and 10-30 parts of kanamycin, wherein the culture reagents are weighed according to parts by weight and are mixed by shaking through a reaction tube, the pH value is controlled to be 6.8-7.5, and the mixed culture reagents are poured into the containing vessel after being treated. The invention can inhibit the growth of other bacteria, which can ensure the normal growth of clostridium botulinum and avoid other bacteria disturbing the observation and analysis visual field of clostridium botulinum of staff.)

1. The food pathogen detection reagent is characterized by comprising a culture reagent and a containing vessel, wherein the culture reagent is stored in the containing vessel;

the culture reagent comprises the following components in parts by weight: 50-100 parts of beef extract powder, 150-300 parts of peptone, 50-100 parts of sodium chloride, 100-200 parts of glucose, 150-300 parts of agar and 10-30 parts of kanamycin, wherein the culture reagents are weighed according to parts by weight and are mixed by shaking through a reaction tube, the pH value is controlled to be 6.8-7.5, and the mixed culture reagents are poured into the containing vessel after being treated;

the holding vessel includes:

the culture device comprises a container (1), wherein the container (1) is used for containing culture reagents, threads (2) are formed in the inner wall of the container (1), positioning grooves (3) are formed in the inner wall of the container (1) at equal intervals along the circumferential direction, and the positioning grooves (3) are located above the threads (2);

the cover plate (4) is screwed on the thread (2);

an injection pipe (5) mounted on the upper surface of the cover plate (4);

a rubber column (6) mounted on the inner wall of the injection pipe (5);

and the positioning component (7) is arranged on the outer side of the upper surface of the cover plate (4) and is clamped with the positioning groove (3) to position the cover plate (4).

2. The food pathogen detection reagent according to claim 1, wherein: the treatment method of the culture reagent comprises the following steps: weighing a proper amount of the culture reagent, adding the culture reagent into distilled water or deionized water, stirring, heating, boiling until the culture reagent is completely dissolved, autoclaving at 121 ℃ for 15min, shaking up, cooling to 60 ℃, adding the yolk saline suspension under aseptic operation, shaking up slightly, and pouring into the container.

3. The food pathogen detection reagent according to claim 2, wherein: in the culture reagent treatment method, the ratio of the culture reagent to distilled water or deionized water is 1: 20.

4. The food pathogen detection reagent according to claim 1, wherein: the number of turns of the thread (2) is at least three.

5. The food pathogen detection reagent according to claim 1, wherein: the cover plate (4) is transparent and is horizontally arranged.

6. The food pathogen detection reagent according to claim 1, wherein: the positioning assembly (7) comprises:

a limiting cylinder (71) mounted on the outer side of the upper surface of the cover plate (4);

the spring (72) is embedded in the inner cavity of the limiting cylinder (71);

and one part of the clamping ball (73) is embedded in the inner cavity of the limiting cylinder (71), and the other part of the clamping ball is clamped with the positioning groove (3) under the action of the spring (72).

7. The food pathogen detection reagent according to claim 6, wherein: the maximum length of the clamping ball (73) extending out of the inner cavity of the limiting cylinder (71) is smaller than the radius of the clamping ball.

Technical Field

The invention relates to the technical field of germ detection, in particular to a food germ detection reagent.

Background

The canned food comprises the following components: refers to a food made from raw materials through processing, canning, sealing, sterilizing or aseptic packaging. The canned food can be stored at room temperature for a long time due to commercial sterility. Food pathogen detection is essential for people to eat safe food;

the method is characterized in that the food pathogenic bacteria inspection needs to be carried out through a plurality of steps of sampling, extracting, marking, culturing, amplifying, analyzing and detecting and the like, various pathogenic bacteria exist in food, escherichia coli and clostridium botulinum are the most common, clostridium botulinum is a virulent pathogenic bacteria, other pathogenic bacteria are difficult to avoid being mixed in the extraction of clostridium botulinum, clostridium botulinum belongs to anaerobic growth, a culture medium plate is usually opened, the injection and culturing of clostridium botulinum are completed, when a target clostridium botulinum is cultured by using the culture medium plate, the mixed pathogenic bacteria can absorb nutrient growth of the culture medium, the growth of clostridium botulinum is influenced, and the observation and analysis visual field of workers are disturbed, so that improvement is needed.

Disclosure of Invention

The invention aims to provide a food germ detection reagent, which at least solves the problems that when clostridium botulinum is extracted in the prior art, nutrition in a mixed germ absorption culture medium can inhibit growth of clostridium botulinum, and observation and analysis of clostridium botulinum are inconvenient.

In order to achieve the purpose, the invention provides the following technical scheme: a food germ detection reagent comprises a culture reagent and a container, wherein the culture reagent is stored in the container;

the culture reagent comprises the following components in parts by weight: 50-100 parts of beef extract powder, 150-300 parts of peptone, 50-100 parts of sodium chloride, 100-200 parts of glucose, 150-300 parts of agar and 10-30 parts of kanamycin, wherein the culture reagents are weighed according to parts by weight and are mixed by shaking through a reaction tube, the pH value is controlled to be 6.8-7.5, and the mixed culture reagents are poured into the containing vessel after being treated;

the holding vessel includes:

the culture device comprises a container, a positioning groove and a screw thread, wherein the container is used for containing a culture reagent, the inner wall of the container is provided with the screw thread, the inner wall of the container is provided with the positioning grooves at equal intervals along the circumferential direction, and the positioning grooves are positioned above the screw thread;

the cover plate is screwed on the thread;

the injection pipe is arranged on the upper surface of the cover plate;

the rubber column is arranged on the inner wall of the injection pipe;

and the positioning assembly is arranged on the outer side of the upper surface of the cover plate and is clamped with the positioning groove to position the cover plate.

Preferably, the method for processing the culture reagent comprises: weighing a proper amount of the culture reagent, adding the culture reagent into distilled water or deionized water, stirring, heating, boiling until the culture reagent is completely dissolved, autoclaving at 121 ℃ for 15min, shaking up, cooling to 60 ℃, adding the yolk saline suspension under aseptic operation, shaking up slightly, and pouring into the container.

Preferably, in the culture reagent treatment method, the ratio of the culture reagent to distilled water or deionized water is 1: 20.

Preferably, the number of turns of the thread is at least three.

Preferably, the cover plate is transparent and is horizontally arranged.

Preferably, the positioning assembly comprises: the limiting cylinder is arranged on the outer side of the upper surface of the cover plate; the spring is embedded in the inner cavity of the limiting cylinder; and one part of the clamping ball is embedded in the inner cavity of the limiting cylinder, and the other part of the clamping ball is clamped with the positioning groove under the action of the spring.

Preferably, the maximum length of the clamping ball extending out of the inner cavity of the limiting cylinder is smaller than the radius of the clamping ball.

The food pathogen detection reagent provided by the invention has the beneficial effects that:

1. the invention can position the rotating cover plate at equal angles by matching the positioning component and the positioning groove, so that the injection pipe can be positioned in multiple directions to realize the uniform injection of clostridium botulinum and prevent air from entering the clostridium botulinum, carbon nitrogen sources, vitamins and growth factors are provided for the clostridium botulinum by peptone and beef extract powder, glucose provides energy, sodium chloride maintains balanced osmotic pressure, agar is used as a coagulant of a culture medium, kanamycin is used as an antibiotic and can inhibit the growth of enterobacter (escherichia coli and the like) and proteus, therefore, the invention can inhibit the growth of other miscellaneous germs, not only ensures the normal growth of the clostridium botulinum, but also avoids the observation and analysis visual fields of other miscellaneous germs workers on the clostridium botulinum.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a right side sectional view of the present invention;

fig. 3 is an enlarged view of the invention at a.

In the figure: 1. vessel, 2, screw thread, 3, locating slot, 4, cover plate, 5, injection pipe, 6, rubber column, 7, locating component, 71, spacing cylinder, 72, spring, 73, card ball.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a food germ detection reagent comprises a culture reagent and a container, wherein the culture reagent is stored in the container;

the culture reagent comprises the following components in parts by weight: 80 parts of beef extract powder, 240 parts of peptone, 80 parts of sodium chloride, 160 parts of glucose, 240 parts of agar and 20 parts of kanamycin, wherein carbon nitrogen sources, vitamins and growth factors are provided for clostridium botulinum by the peptone and the beef extract powder, the glucose provides energy, the sodium chloride maintains balanced osmotic pressure, the agar is used as a coagulant of a culture medium, the kanamycin is used as an antibiotic and can inhibit the growth of enterobacter (escherichia coli and the like) and proteus, the culture reagents are weighed according to parts by weight and are mixed by shaking of a reaction tube, the pH value is controlled to be 6.8-7.5, and the mixed culture reagents are poured into a containing vessel after being treated;

the method for processing the culture reagent comprises the following steps: weighing a proper amount of culture reagent, adding the culture reagent into distilled water or deionized water, wherein the ratio of the culture reagent to the distilled water or the deionized water is 1:20, stirring, heating, boiling until the culture reagent is completely dissolved, carrying out autoclaving at 121 ℃ for 15min, shaking up, cooling to 60 ℃, adding the yolk saline suspension under aseptic operation, shaking up gently, and pouring into a container;

the container comprises a container 1, a thread 2, a positioning groove 3, a cover plate 4, an injection pipe 5, a rubber column 6 and a positioning assembly 7, wherein the container 1 of the container 1 is used for containing culture reagents, the inner wall of the container 1 is provided with the thread 2, the number of turns of the thread 2 is at least three, when the cover plate 4 rotates for one turn, the cover plate 4 is ensured not to be separated from the thread 2, further, the air tightness of the container 1 is covered, air is prevented from entering, the positioning groove 3 is arranged on the inner wall of the container 1 along the circumferential direction at equal intervals, the positioning groove 3 is positioned above the thread 2, the cover plate 4 is screwed on the thread 2, the cover plate 4 is transparent and is horizontally arranged, when the microscope is used for observation, the microscope can be conveniently adjusted to be vertical to the cover plate 4, the cover plate 4 is prevented from generating refraction to influence on observation of clostridium botulinum in the container 1, the injection pipe 5 is arranged on the upper surface of the cover plate 4, the rubber column 6 is arranged on the inner wall of the injection pipe 5, utilize the high elastic characteristic of rubber column 6, after the syringe needle that is equipped with clostridium botulinum pierces through rubber column 6 and pours into household utensils 1 into, rubber column 6 still can play sealed effect to household utensils 1, and locating component 7 installs in the upper surface outside of apron 4, and fixes a position apron 4 with locating groove 3 joint.

The positioning assembly 7 comprises a limiting cylinder 71, a spring 72 and a clamping ball 73, the limiting cylinder 71 is installed on the outer side of the upper surface of the cover plate 4, the spring 72 is embedded in an inner cavity of the limiting cylinder 71, the spring 72 is a rotary spring and elastically deforms after being stretched or extruded, the spring recovers to an initial state after external force is removed, one part of the clamping ball 73 is embedded in the inner cavity of the limiting cylinder 71, the other part of the clamping ball 73 is clamped with the positioning groove 3 under the action of the spring 72, the maximum length of the clamping ball 73 extending out of the inner cavity of the limiting cylinder 71 is smaller than the radius of the clamping ball 73, and therefore the clamping ball 73 can not be clamped between the limiting cylinder 71 and the inner wall of the vessel 1 when the cover plate 4 is rotated, and the clamping ball 73 can be retracted into the limiting cylinder 71.

The detailed connection means is a technique known in the art, and the following mainly describes the working principle and process, and the specific operation is as follows.

Step one, when clostridium botulinum needs to be injected into a vessel 1, a needle head penetrates through a rubber column 6 to be driven in, due to the fact that clostridium botulinum has strong inertia, a cover plate 4 is rotated, a clamping ball 73 rolls into a limiting cylinder 71 under the blocking of a positioning groove 3, the clamping ball 73 is contacted with the inner wall of the vessel 1 to position the cover plate 4 until the clamping ball 73 moves to the next positioning groove 3, a spring 72 pushes the clamping ball 73 to be inserted into the positioning groove 3 under the action of self elasticity, the cover plate 4 is positioned, clostridium botulinum is driven into one part again, and in this way, clostridium botulinum is uniformly driven into the vessel 1, so that clostridium botulinum uniformly exists in the vessel 1, and clostridium botulinum is uniformly distributed and can fully absorb nutrition;

step two, keeping the constant temperature of the vessel 1 at 35-37 ℃ to achieve the optimal growth temperature of clostridium botulinum, providing a carbon nitrogen source, vitamins and growth factors for clostridium botulinum by peptone and beef extract powder, providing energy for glucose, maintaining balanced osmotic pressure by sodium chloride, taking agar as a coagulant of a culture medium, taking kanamycin as an antibiotic, and inhibiting the growth of enterobacter (escherichia coli and the like) and proteus to ensure that clostridium botulinum stably grows;

the invention can inhibit the growth of other bacteria mixed in the utensil 1, ensure the normal growth of the clostridium botulinum, facilitate the observation and analysis of the clostridium botulinum, and can inject the clostridium botulinum into the culture medium under the anaerobic condition, thereby providing an anaerobic environment, being beneficial to the growth of the clostridium botulinum and having strong practicability.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.