阅读说明：本技术 一种抗真菌药敏试剂mic值检测方法 (Method for detecting MIC value of antifungal drug sensitive reagent ) 是由 刘维达 郑海林 王琼 宋歌 于 2021-08-27 设计创作，主要内容包括：本发明公开了一种抗真菌药敏试剂MIC值检测方法,先准备深部感染药敏试剂、浅部感染药敏试剂和外用药敏试剂；将上述三种药敏试剂分别加入三份琼脂中；将上述三种含有药敏试剂的琼脂分别装入三个注入器中；准备琼脂平板,并在琼脂平板中加入待测真菌；在琼脂平板中等间距打三个孔,通过三个注入器将三种含有药敏试剂的琼脂分别注入到三个孔中；待真菌生长后,观测抑菌环大小并换算出MIC值；本发明首次将抗真菌药物按深部感染、浅部感染和外用三类进行分别组合；将药物融入琼脂使其与培养环境一致,并在琼脂平板培养基上打出相等大小的孔洞,以此观察抑菌环,最终获得客观准确的MIC值。(The invention discloses an MIC value detection method of an antifungal drug sensitive reagent, which comprises the steps of firstly preparing a deep infection drug sensitive reagent, a shallow infection drug sensitive reagent and an external drug sensitive reagent; adding the three drug sensitive reagents into three parts of agar respectively; loading the three kinds of agar containing drug sensitive reagents into three injectors respectively; preparing an agar plate, and adding fungi to be detected into the agar plate; punching three holes at equal intervals on an agar plate, and respectively injecting three kinds of agar containing the drug sensitive reagent into the three holes through three injectors; after the fungi grow, observing the size of an antibacterial ring and converting the size into an MIC value; the invention firstly combines antifungal medicines according to deep infection, superficial infection and external application; the medicine is melted into the agar to be consistent with the culture environment, holes with the same size are punched on the agar plate culture medium, the bacteriostasis ring is observed, and objective and accurate MIC values are finally obtained.)

1. A method for detecting the MIC value of an antifungal drug sensitive reagent is characterized by comprising the following steps:

preparing a deep infection drug sensitive reagent, a shallow infection drug sensitive reagent and an external drug sensitive reagent;

adding the three drug sensitive reagents into three parts of agar respectively;

thirdly, the three kinds of agar containing the drug sensitive reagent are respectively filled into three injectors;

preparing an agar plate, and adding the fungus to be detected into the agar plate;

fifthly, punching three holes in the agar plate at equal intervals, and respectively injecting three kinds of agar containing the drug sensitive reagent into the three holes through three injectors;

sixthly, observing the size of the antibacterial ring after the fungi grow and converting the size into an MIC value.

2. The method for detecting the MIC value of an antifungal drug-sensitive reagent in accordance with claim 1, wherein:

in the third step, the three kinds of agars containing the drug sensitive reagents are equal, and the content of the drug sensitive reagents in each kind of agars is the same.

3. The method for detecting the MIC value of an antifungal drug-sensitive reagent in accordance with claim 1, wherein: three holes on the agar plate are arranged in a row, three injectors are fixed on the same bracket at equal intervals to form a triple structure, and the intervals of the three injectors are equal to the intervals of the three holes on the agar plate.

4. The method for detecting the MIC value of an antifungal drug-sensitive reagent in accordance with claim 1, wherein: the agar plate is of a circular structure, three holes in the agar plate are annularly arranged around the center of the agar plate at equal intervals, three injectors are fixed on the same bracket and are annularly arranged around the center of the bracket at equal intervals, and the center distance of the three injectors is equal to that of the three holes in the agar plate.

Technical Field

The invention relates to a method for detecting an MIC value of an antifungal drug sensitive reagent, belonging to the technical field of biological medicine.

Background

Antifungal drugs need to pass a fungus culture experiment to detect the MIC value of the drugs; mic (minimal inhibitory concentration) means the minimum inhibitory concentration, which is an index for measuring the antibacterial activity of antibacterial drugs and means the minimum drug concentration that can inhibit the growth of pathogenic bacteria in a culture medium after culturing bacteria in vitro for 18 to 24 hours; the existing antifungal drug sensitive reagent has a single MIC value detection mode, and the detection result lacks contrast, so that the actual effect of the drug sensitive reagent is difficult to reflect.

Disclosure of Invention

In view of the above technical problems, the present invention aims to: provides a method for detecting MIC value of antifungal drug sensitive reagent.

The technical solution of the invention is realized as follows: a method for detecting the MIC value of an antifungal drug sensitive reagent comprises the following steps:

preparing a deep infection drug sensitive reagent, a shallow infection drug sensitive reagent and an external drug sensitive reagent;

adding the three drug sensitive reagents into three parts of agar respectively;

thirdly, the three kinds of agar containing the drug sensitive reagent are respectively filled into three injectors;

preparing an agar plate, and adding the fungus to be detected into the agar plate;

fifthly, punching three holes in the agar plate at equal intervals, and respectively injecting three kinds of agar containing the drug sensitive reagent into the three holes through three injectors;

sixthly, observing the size of the antibacterial ring after the fungi grow and converting the size into an MIC value.

Preferably, in the third step, the three kinds of agars containing the drug sensitive reagents are equal, and the content of the drug sensitive reagents in each kind of agars is the same.

Preferably, three holes on the agar plate are arranged in a row, three injectors are fixed on the same bracket at equal intervals to form a triple structure, and the intervals of the three injectors are equal to the intervals of the three holes on the agar plate.

Preferably, the agar plate is of a circular structure, three holes in the agar plate are annularly arranged around the center of the agar plate at equal intervals, the three injectors are fixed on the same bracket and are annularly arranged around the center of the bracket at equal intervals, and the center distance of the three injectors is equal to that of the three holes in the agar plate.

Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:

the invention provides a method for detecting an MIC value of an antifungal drug sensitive reagent, which combines antifungal drugs according to deep infection, superficial infection and external application for the first time; the medicine is melted into the agar to be consistent with the culture environment, holes with the same size are punched on the agar plate culture medium, the bacteriostasis ring is observed, and objective and accurate MIC values are finally obtained.

Detailed Description

The present invention will be described with reference to examples.

The invention relates to a method for detecting an MIC value of an antifungal drug sensitive reagent, which comprises the following steps:

preparing a deep infection drug sensitive reagent, a shallow infection drug sensitive reagent and an external drug sensitive reagent.

② adding the above-mentioned three drug sensitive reagents into three portions of agar respectively.

Thirdly, the three kinds of agar containing the drug sensitive reagent are respectively filled into three injectors; the three agars containing the drug sensitive reagent were in equal amounts, and the content of the drug sensitive reagent in each agar was the same.

Preparing an agar plate, and adding the fungus to be detected into the agar plate.

Fifthly, punching three holes on the agar plate at equal intervals, and respectively injecting three kinds of agar containing the drug sensitive reagent into the three holes through three injectors.

Sixthly, observing the size of the bacteriostatic ring after the fungi grow, and converting the size into an MIC value.

In a preferred embodiment, the three holes on the agar plate are arranged in a row, the three injectors are fixed on the same bracket at equal intervals to form a triple structure, and the intervals between the three injectors are equal to the intervals between the three holes on the agar plate.

In another preferred embodiment, the agar plate has a circular structure, three holes on the agar plate are arranged in an annular shape at equal intervals around the center of the agar plate, three injectors are fixed on the same bracket, three injectors are arranged in an annular shape at equal intervals around the center of the bracket, and the center distance of the three injectors is equal to the center distance of the three holes on the agar plate.

The above-mentioned embodiments are merely illustrative of the technical idea and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the scope of the present invention.