阅读说明：本技术 一种测定植物叶中甲酸脱氢酶活性及其评价的方法 (Method for determining activity of formate dehydrogenase in plant leaves and evaluating formate dehydrogenase in plant leaves ) 是由 苏玉红 何晓红 杨玉霞 李晓娟 于 2020-06-18 设计创作，主要内容包括：本发明公开了一种测定植物叶中甲酸脱氢酶活性及其评价的方法,以高温处理的方式,消除叶提取液中甲酸脱氢酶的活性,根据甲酸的化学性质,采用电位滴定法,通过测定外加甲醛后植物新鲜提取液和失活提取液中甲醛含量的减少值,及外加甲醛前后植物新鲜提取液和失活提取液中酸度变化的差异,进而定量评估植物叶中甲酸脱氢酶的活性大小。实验结果表明,植物质量和提取液比例为1:4时,当甲醛浓度为350 mg·L～(-1)时甲酸脱氢酶活性大小依次为：绿萝>牡丹吊兰>芦荟；当甲醛浓度为500～750 mg·L～(-1)时甲酸脱氢酶活性大小依次为：牡丹吊兰>绿萝,芦荟叶提取液未检测到甲酸脱氢酶活性。(The invention discloses a method for measuring the activity of formate dehydrogenase in plant leaves and evaluating the activity of the formate dehydrogenase in the plant leaves, which eliminates the activity of the formate dehydrogenase in a leaf extracting solution in a high-temperature treatment mode, and quantitatively evaluates the activity of the formate dehydrogenase in the plant leaves by measuring the reduction values of formaldehyde content in a fresh plant extracting solution and an inactivated extracting solution after formaldehyde is added and the difference of acidity change in the fresh plant extracting solution and the inactivated extracting solution before and after the formaldehyde is added by adopting a potentiometric titration method according to the chemical property of formic acid. The experimental result shows that when the ratio of the plant mass to the extraction solution is 1:4, the concentration of the formaldehyde is 350 mg.L ‑1 The activity of the formic acid dehydrogenase is as follows in sequence: scindapsus aureus (Gloiopeltis scindapsus) Roxb>Peony chlorophytum comosum>Aloe vera; when the concentration of formaldehyde is 500-750 mg.L ‑1 The activity of the formic acid dehydrogenase is as follows in sequence: peony chlorophytum comosum>The activity of formate dehydrogenase is not detected in the extract of scindapsus aureus and aloe leaves.)