阅读说明：本技术 疫苗和方法 (Vaccines and methods ) 是由 乔纳森·卢克·希尼 西蒙·弗罗斯特 拉尔夫·瓦格纳 贝内迪克特·阿斯巴赫 丽贝卡·金斯利 于 2019-09-27 设计创作，主要内容包括：描述了用于对能够诱导对病原体的广泛中和免疫应答和相关T细胞应答的经优化抗原性病原体多肽进行鉴定的方法,以及编码这样的多肽的核酸序列。还描述了用于确定在用经优化抗原性病原体多肽或编码经优化病原体多肽的核酸免疫接种之后在对象中是否诱导了广泛中和免疫应答的方法。还描述了核酸分子、多肽、载体、细胞、融合蛋白、药物组合物,及其作为针对病原体,特别是针对新出现或再出现的病原体(特别是RNA病毒)的疫苗的用途。(Methods for identifying optimized antigenic pathogen polypeptides capable of inducing broadly neutralizing immune responses and associated T cell responses to pathogens are described, as well as nucleic acid sequences encoding such polypeptides. Also described are methods for determining whether a broadly neutralizing immune response is induced in a subject following immunization with an optimized antigenic pathogen polypeptide or a nucleic acid encoding an optimized pathogen polypeptide. Also described are nucleic acid molecules, polypeptides, vectors, cells, fusion proteins, pharmaceutical compositions, and their use as vaccines against pathogens, in particular against emerging or emerging pathogens (in particular RNA viruses).)