阅读说明：本技术 用于测序的引物寡核苷酸 (Primer oligonucleotides for sequencing ) 是由 P.加蒂-拉夫兰科尼 P.巴尔丁 J.M.鲍特尔 于 2019-12-09 设计创作，主要内容包括：多核苷酸测序方法采用了测序寡核苷酸,该测序寡核苷酸以比表面核苷酸更大的亲和力与模板多核苷酸链的游离3’末端部分杂交。可将此类测序寡核苷酸用作引物,通过利用模板链作为模板延伸测序寡核苷酸来确定索引序列的序列。采用此类测序寡核苷酸的测序过程提供了足够强的信号来确定索引序列的序列。(The polynucleotide sequencing method employs a sequencing oligonucleotide that hybridizes to the free 3' terminal portion of the template polynucleotide strand with greater affinity than the surface nucleotides. Such sequencing oligonucleotides can be used as primers to determine the sequence of the index sequence by extending the sequencing oligonucleotide using the template strand as a template. Sequencing processes using such sequencing oligonucleotides provide sufficiently strong signals to determine the sequence of the index sequence.)