阅读说明：本技术 一种益生元组合物及其应用 (Prebiotic composition and application thereof ) 是由 郝占西 曾宪维 杨新球 魏远安 李华军 吴少辉 王国盼 于 2021-07-13 设计创作，主要内容包括：本发明公开了一种益生元组合物及其应用,属于生物技术领域。本发明的益生元组合物包括低聚果糖和低聚半乳糖,本发明还提供了所述益生元组合物的制备方法及应用。采用该制备方法得到的益生元组合物能用于制备改善肥胖的产品、降低血清脂多糖和血清D-乳酸的产品、降低体重增量的产品、降低附睾脂肪的产品、用于新兴微生态医疗领域的产品,这些产品能有效避免药物减肥的副作用,精准发挥益生元组合物的生理作用,克服益生元应用层面的模糊性、随意性等局限,为精准营养与新兴微生态医疗等生物医药领域的应用提供典型示范,有助于改善肥胖症的预防控制与治疗康复。(The invention discloses a prebiotic composition and application thereof, belonging to the technical field of biology. The prebiotic composition comprises fructo-oligosaccharide and galacto-oligosaccharide, and the invention also provides a preparation method and application of the prebiotic composition. The prebiotic composition obtained by the preparation method can be used for preparing products for improving obesity, products for reducing serum lipopolysaccharide and serum D-lactic acid, products for reducing weight gain, products for reducing epididymal fat and products for emerging micro-ecological medical treatment fields, can effectively avoid the side effect of drug weight reduction, accurately play the physiological effect of the prebiotic composition, overcome the limitations of ambiguity, randomness and the like of prebiotic application level, provide typical demonstration for the application of accurate nutrition, emerging micro-ecological medical treatment and other biological medicine fields, and is beneficial to improving the prevention control and treatment rehabilitation of obesity.)

1. A prebiotic composition comprising a fructooligosaccharide and a galactooligosaccharide.

2. The prebiotic composition according to claim 1, wherein the fructooligosaccharide is present in an amount of 100 to 900 parts by mass, and the galactooligosaccharide is present in an amount of 100 to 900 parts by mass.

3. The prebiotic composition according to claim 1, wherein the fructooligosaccharide is contained in an amount of 300 to 900 parts by mass and the galactooligosaccharide is contained in an amount of 100 to 700 parts by mass.

4. The prebiotic composition according to claim 1, wherein the fructooligosaccharide is present in an amount of 500 to 900 parts by mass and the galactooligosaccharide is present in an amount of 100 to 500 parts by mass.

5. The prebiotic composition according to claim 1, wherein the fructooligosaccharide is present in an amount of 700 to 900 parts by mass and the galactooligosaccharide is present in an amount of 100 to 300 parts by mass.

6. Use of a prebiotic composition according to any one of claims 1 to 5 in the manufacture of a product for the amelioration of obesity.

7. Use of prebiotic composition according to any one of claims 1 to 5 in the manufacture of a product for lowering serum lipopolysaccharide and serum D-lactic acid.

8. Use of a prebiotic composition according to any one of claims 1 to 5 in the manufacture of a product for reducing weight gain.

9. Use of a prebiotic composition according to any one of claims 1 to 5 in the manufacture of a product for reducing epididymal fat.

10. Use of a prebiotic composition according to any one of claims 1 to 5 in the manufacture of a product for use in the emerging field of micro-ecological medical treatment.

Technical Field

The invention belongs to the technical field of biology, and particularly relates to a prebiotic composition and application thereof.

Background

Obesity is a chronic, complex disease, and is closely related to diseases such as hyperlipidemia, type 2 diabetes, fatty liver, cardiovascular diseases, cancer, etc. which are common in the present society. Excessive accumulation of fat can have adverse effects on the metabolism, physiological activities and mental health of the body. Therefore, weight loss and prevention of obesity are important areas of research in the management of many chronic diseases.

For a long time, prevention and treatment means for obesity have been sought. Orlistat (Orlistat), which is the current relatively popular weight loss product, was approved in europe in 1998 and is the only FDA approved drug for long-term obesity treatment that inhibits gastrointestinal and pancreatic lipases, blocks hydrolysis of triglycerides, and reduces absorption of ingested fat. However, while reducing body weight, there are some inevitable side effects such as fecal incontinence, excessive defecation, oily stool, etc., fat-soluble vitamins need to be supplemented to avoid vitamin deficiency, and intestinal microbial diversity and abundance are also reduced.

To avoid the side effects of drug weight loss, it is considered to control obesity by another means. Prebiotics are a class of substances that are selectively utilized by microorganisms to confer a health benefit on the host, including Fructooligosaccharides (FOS), Galactooligosaccharides (GOS), and the like. Regular intake of prebiotics such as fructo-oligosaccharide and galacto-oligosaccharide can increase short chain fatty acids produced by intestinal flora and increase release of peptides for eliminating fat.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a prebiotic composition which can be used for preparing products for improving obesity, products for reducing serum lipopolysaccharide and serum D-lactic acid, products for reducing weight gain, products for reducing epididymal fat and products for emerging microecological medical field.

In order to achieve the purpose, the invention adopts the technical scheme that: a prebiotic composition comprising a fructooligosaccharide and a galactooligosaccharide.

The prebiotic composition obtained by the technical scheme of the invention can be used for preparing products for improving obesity, products for reducing serum lipopolysaccharide and serum D-lactic acid, products for reducing weight gain, products for reducing epididymal fat and products for the field of emerging micro-ecological medical treatment, can effectively avoid the side effect of drug weight loss, accurately play the physiological action of the prebiotic composition, overcome the limitations of ambiguity, randomness and the like of the prebiotic application level, provide typical demonstration for the application of the fields of biomedicine such as accurate nutrition and emerging micro-ecological medical treatment and the like, and is beneficial to improving the prevention control and treatment rehabilitation of obesity.

In a preferred embodiment of the prebiotic composition of the present invention, the fructooligosaccharide is 100 to 900 parts by mass, and the galactooligosaccharide is 100 to 900 parts by mass.

In a preferred embodiment of the prebiotic composition of the present invention, the fructooligosaccharide is 300 to 900 parts by mass, and the galactooligosaccharide is 100 to 700 parts by mass.

In a preferred embodiment of the prebiotic composition of the present invention, the fructooligosaccharide is 500 to 900 parts by mass, and the galactooligosaccharide is 100 to 500 parts by mass.

In a preferred embodiment of the prebiotic composition of the present invention, the fructooligosaccharide is 700 to 900 parts by mass, and the galactooligosaccharide is 100 to 300 parts by mass.

The fructo-oligosaccharide is a natural active substance, can bidirectionally regulate the micro-ecological balance of a human body, and is a typical super-strong bifidus factor; the galactooligosaccharide is a nutrient source and an effective proliferation factor of beneficial bacteria such as bifidobacterium, acidophilic lactobacillus and the like in human intestinal tracts, and can improve the digestion and absorption functions of the human intestinal tracts. The invention can achieve the effect which cannot be achieved by single use by matching fructo-oligosaccharide and galacto-oligosaccharide. The preference for the ratio is mainly based on the combined reducing effect of the prebiotic composition on the individual obesity indicators. Wherein, the prebiotic composition with the mass part of the fructo-oligosaccharide of 900 parts and the mass part of the galacto-oligosaccharide of 100 parts has the best comprehensive reduction effect on each obesity index.

In addition, the invention also provides application of the prebiotic composition in preparing products for improving obesity.

In addition, the invention also provides application of the prebiotic composition in preparing products for reducing serum lipopolysaccharide and serum D-lactic acid.

In addition, the invention also provides application of the prebiotic composition in preparing products for reducing weight gain.

In addition, the invention also provides application of the prebiotic composition in preparation of products for reducing epididymis fat.

In addition, the invention also provides application of the prebiotic composition in preparation of products used in the emerging field of microecological medical treatment.

Compared with the prior art, the invention has the beneficial effects that: the invention provides a prebiotic composition comprising fructooligosaccharides and galactooligosaccharides. Meanwhile, the invention also provides application of the prebiotic composition in preparing products for improving obesity, products for reducing serum lipopolysaccharide and serum D-lactic acid, products for reducing weight gain, products for reducing epididymal fat and products for emerging micro-ecological medical field. The prebiotic composition obtained by the technical scheme of the invention can effectively improve obesity index, avoid side effect of drug weight loss, accurately exert physiological effect of the prebiotic composition, overcome the limitations of ambiguity, randomness and the like of prebiotic application level, provide typical demonstration for application in the biomedical fields of accurate nutrition, emerging micro-ecological medical treatment and the like, and is beneficial to improvement of prevention control and treatment rehabilitation of obesity.

Drawings

FIG. 1 is a graph of the effect of prebiotic compositions of example 6 on weight gain in an obese mouse model;

FIG. 2 is a graph of the effect of prebiotic compositions of example 6 on epididymal fat index in an obese mouse model;

FIG. 3 is a graph of the effect of prebiotic compositions of example 6 on Lee index in an obese mouse model;

FIG. 4 is a graph of the effect of prebiotic compositions of example 6 on serum lipopolysaccharide content in a mouse model of obesity;

FIG. 5 is a graph of the effect of prebiotic compositions of example 6 on serum D-lactic acid levels in obese mouse models.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

Example 1

The prebiotic composition provided by the embodiment of the invention comprises the following components in percentage by mass: 900 parts of fructo-oligosaccharide and 100 parts of galacto-oligosaccharide.

The prebiotic composition described in this example was prepared as follows:

taking 900g of fructo-oligosaccharide and 100g of galacto-oligosaccharide, sieving the two, uniformly mixing, adding normal saline to dissolve the mixture until the concentration is 75 percent, and finally sterilizing to obtain the prebiotic composition 1.

Example 2

The prebiotic composition provided by the embodiment of the invention comprises the following components in percentage by mass: 700 parts of fructo-oligosaccharide and 300 parts of galacto-oligosaccharide.

The prebiotic composition described in this example was prepared as follows:

taking 700g of fructo-oligosaccharide and 300g of galacto-oligosaccharide, sieving the two, uniformly mixing, adding physiological saline to dissolve the mixture until the concentration is 75 percent, and finally sterilizing to obtain the prebiotic composition 2.

Example 3

The prebiotic composition provided by the embodiment of the invention comprises the following components in percentage by mass: 500 parts of fructo-oligosaccharide and 500 parts of galacto-oligosaccharide.

The prebiotic composition described in this example was prepared as follows:

taking 500g of fructo-oligosaccharide and 500g of galacto-oligosaccharide, sieving the two, uniformly mixing, adding normal saline to dissolve the mixture until the concentration is 75 percent, and finally sterilizing to obtain the prebiotic composition 3.

Example 4

The prebiotic composition provided by the embodiment of the invention comprises the following components in percentage by mass: 300 parts of fructo-oligosaccharide and 700 parts of galacto-oligosaccharide.

The prebiotic composition described in this example was prepared as follows:

taking 300g of fructo-oligosaccharide and 700g of galacto-oligosaccharide, sieving the two, uniformly mixing, adding normal saline to dissolve the mixture until the concentration is 75 percent, and finally sterilizing to obtain the prebiotic composition 4.

Example 5

The prebiotic composition provided by the embodiment of the invention comprises the following components in percentage by mass: 100 parts of fructo-oligosaccharide and 900 parts of galacto-oligosaccharide.

The prebiotic composition described in this example was prepared as follows:

taking 100g of fructo-oligosaccharide and 900g of galacto-oligosaccharide, sieving the two, uniformly mixing, adding normal saline to dissolve the mixture until the concentration is 75 percent, and finally sterilizing to obtain the prebiotic composition 5.

Comparative example 1

Comparative example 1 was the preparation of fructooligosaccharides.

The preparation method of the fructo-oligosaccharide of the comparative example is as follows:

1000g of fructo-oligosaccharide is taken, sieved and dissolved by adding normal saline with the concentration of 75 percent, and finally, the fructo-oligosaccharide is obtained by sterilization.

Comparative example 2

Comparative example 2 was the preparation of galactooligosaccharides.

The preparation method of galactooligosaccharide described in this comparative example is as follows:

1000g of galacto-oligosaccharide is taken, sieved and dissolved by adding normal saline with the concentration of 75 percent, and finally, the galacto-oligosaccharide is obtained by sterilization.

Example 6

The embodiment of the invention researches the application of the prebiotic composition in preparing products for improving obesity, which comprises the following contents:

(1) establishing a mouse model: 80 mice with 5-week-old males, the average weight of 22 +/-2 g and the SPF grade of C57BL/6 are taken, after one week of ordinary feed feeding, the 80 mice are randomly divided into 8 groups, each group comprises 10 mice, the normal group (N) is fed with the ordinary feed, and the other groups are fed with 60% high-fat feed. Mice of the normal group (N) and the model group (M) were gavaged with 0.2mL of physiological saline daily, the positive control group (Y) was gavaged with orlistat physiological saline at a dose of 120mg/kg/d, the fructooligosaccharide of comparative example 1 was gavaged with 1.7g/kg/d for the fructooligosaccharide low dose group (FL), the fructooligosaccharide of comparative example 1 was gavaged with 8.5g/kg/d for the fructooligosaccharide high dose group (FH), the galactooligosaccharide of comparative example 2 was gavaged with 1.7g/kg/d for the galactooligosaccharide low dose Group (GL), the galactooligosaccharide of comparative example 2 was gavaged with 8.5g/kg/d for the galactooligosaccharide of comparative example 2, and the prebiotic composition of examples 1-5 was gavaged with 8.5g/kg/d for the prebiotic composition of 1-5 (S), the gavage treatment time was 6 weeks.

(2) Calculation of weight gain: the weight gain of mice was calculated from these two data and the results of the weight gain were shown in table 1, wherein the weight gain of the composition 1 obtained in example 1 was the least increased in weight, and the normal group, model group, positive control group, low-dose group of fructo-oligosaccharide, high-dose group of fructo-oligosaccharide, low-dose group of galacto-oligosaccharide, and high-dose group of galacto-oligosaccharide were plotted in a bar graph, as shown in fig. 1.

As can be seen from fig. 1, the weight gain of the mice of the prebiotic composition group (S) and the positive control group (Y) remained equal, and both of them significantly reduced the weight gain of the model group mice; the weight gain of the low-dose group of Fructooligosaccharides (FL) was greater than that of the high-dose group of Fructooligosaccharides (FH) and the weight gain of the low-dose group of Galactooligosaccharides (GL) was less than that of the high-dose group of Galactooligosaccharides (GH), indicating that it is not a simple dose-dependent relationship that either fructooligosaccharides or galactooligosaccharides alone reduce the weight gain of mice.

(3) Calculating the epididymis index: after the mice were sacrificed, the intact epididymal fat was removed, the epididymal index was calculated according to the formula of epididymal fat index (g)/body weight (g), the obtained result of the epididymal index is shown in table 1, and the composition 1 obtained in example 1, in which the epididymal index was the smallest, was plotted as a histogram with the normal group, the model group, the positive control group, the low-dose group of fructooligosaccharide, the high-dose group of fructooligosaccharide, the low-dose group of galactooligosaccharide, and the high-dose group of galactooligosaccharide, as shown in fig. 2.

As can be seen from fig. 2, the fat index of the epididymis of the prebiotic composition group (S) is kept equal to the fat index of the epididymis of the positive control group (Y), and both of them can significantly reduce the fat index of the epididymis of the mice of the model group; the effect of the low-dose fructo-oligosaccharide (FL) and low-dose galacto-oligosaccharide (GL) on the reduction of epididymal fat index in mice was superior to that of the high-dose fructo-oligosaccharide (FH) and high-dose galacto-oligosaccharide (GH), indicating that the epididymal fat index was not decreased with increasing dose of fructo-oligosaccharide or galacto-oligosaccharide.

(4) Calculation of Lee index: at the end of the experiment, the mouse mass and body length were measured, and the Lee index was calculated according to the Lee index ═ body mass (g) ^ (1/3) × 1000/body length (cm), and the Lee index results obtained are shown in table 1, and composition 1 obtained in example 1, in which the Lee index was the smallest, and normal group, model group, positive control group, low-dose group of fructooligosaccharide, high-dose group of fructooligosaccharide, low-dose group of galactooligosaccharide, and high-dose group of galactooligosaccharide were plotted as a bar graph, as shown in fig. 3.

As can be seen from fig. 3, the Lee index of the mice of prebiotic composition group (S) remained equal to that of the positive control group (Y), and both significantly decreased the Lee index of the mice of the model group; the effect of the low-dose group of Fructooligosaccharides (FL) and the low-dose group of Galactooligosaccharides (GL) on the reduction of the mouse Lee index was slightly better than the effect of the high-dose group of Fructooligosaccharides (FH) and the high-dose group of Galactooligosaccharides (GH) on the reduction of the mouse Lee index.

(5) Measurement of serum lipopolysaccharide and serum D-lactic acid content: after a mouse is fasted for 12 hours, the eyeball is picked up and blood is collected, the mouse is kept stand for 60 minutes at room temperature and centrifuged for 15 minutes at 3000rpm, serum is sucked and placed in a small test tube, the contents of lipopolysaccharide and D-lactic acid in the supernatant are detected by an enzyme-linked immunosorbent assay, the obtained results of the contents of the lipopolysaccharide and the D-lactic acid in the serum are shown in table 1, the composition 1 obtained in the example 1 with the minimum content of the lipopolysaccharide and the D-lactic acid in the serum in the composition, a normal group, a model group, a positive control group, a low-dose fructooligosaccharide group, a high-dose fructooligosaccharide group, a low-dose galactooligosaccharide group and a high-dose galactooligosaccharide group are drawn into a column diagram, the content of the lipopolysaccharide in the serum is shown in figure 4, and the content of the D-lactic acid in the serum is shown in figure 5.

As can be seen from FIGS. 4 and 5, the prebiotic composition (S) has a certain effect of reducing serum lipopolysaccharide and serum D-lactic acid, and can significantly reduce the contents of serum lipopolysaccharide and serum D-lactic acid in the model mice.

Table 1: prebiotic composition Experimental data sheet

As can be seen from table 1, the prebiotic composition can significantly reduce the weight gain of the model group mice, reduce the epididymis index and Lee index of the model group mice, and reduce the content of serum lipopolysaccharide and serum D-lactic acid in the model group mice, and the reduction range of the prebiotic composition on the weight gain, the epididymis index, the Lee index, the serum lipopolysaccharide content and the serum D-lactic acid content is related to the weight parts of fructooligosaccharide and galactooligosaccharide in the prebiotic composition, and the data in table 1 shows that the reduction range of the prebiotic composition 1 to prebiotic composition 5 prepared in examples 1 to 5 on the whole is the greatest when 900 parts of fructooligosaccharide and 100 parts of galactooligosaccharide are used. Therefore, the experimental data of prebiotic composition 1 was further selected and plotted as bar graphs 1-5 with the experimental data of normal group, model group, positive control group, low-dosage group of fructooligosaccharide, high-dosage group of fructooligosaccharide, low-dosage group of galactooligosaccharide, and high-dosage group of galactooligosaccharide.

As can be seen from fig. 1 to 5, overall, the effect of the prebiotic composition group (S) on the weight gain, the epididymis index, the Lee index, the serum lipopolysaccharide content and the serum D-lactic acid content is almost equal to that of the positive control group (Y), and both of them can significantly reduce the weight gain, the epididymis index, the Lee index, the serum lipopolysaccharide content and the serum D-lactic acid content of the model group mice; meanwhile, the fructo-oligosaccharide low-dose group (FL), the galacto-oligosaccharide low-dose Group (GL), the fructo-oligosaccharide high-dose group (FH) and the galacto-oligosaccharide high-dose Group (GH) have certain effects on the weight gain, epididymis index, Lee index, serum lipopolysaccharide content and serum D-lactic acid content of the mice in the model group, but the effects are evaluated on the whole, and the effects are not good in the reduction effect of the prebiotic composition group (S) on each index. The results indicate that the fructo-oligosaccharide and the galacto-oligosaccharide have certain effect of improving the obesity index, but the overall effect is not superior to the effect of the prebiotic composition on improving the obesity index.

Finally, it should be noted that the above embodiments are intended to illustrate the technical solutions of the present invention and not to limit the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.