阅读说明：本技术 疫苗组合物 (Vaccine composition ) 是由 苏米·比斯瓦斯 金菁 丽贝卡·爱丽丝·达布斯 吉纳维芙·玛丽·凯瑟琳·拉贝 于 2019-05-03 设计创作，主要内容包括：本发明涉及疫苗组合物,最特别地是其中抗原组分大(例如超过50kDa)的疫苗组合物,或是多聚体(即由亚单位组成)的疫苗组合物。这样的抗原组分特别令人感兴趣,因为它们可能代表来自目前无法针对其接种疫苗的病原体的抗原组分。本发明涉及包含展示抗原组分的粒子的组合物,其中所述组合物包含含有第一肽标签的抗原组分和含有第二肽标签的部分,其中所述抗原组分和所述部分通过所述第一和第二肽标签之间的异肽键连接,并且其中所述抗原组分超过50kDa,或者可选地是多聚体。(The present invention relates to vaccine compositions, most particularly vaccine compositions in which the antigenic component is large (e.g. over 50kDa), or multimeric (i.e. composed of subunits). Such antigenic components are of particular interest as they may represent antigenic components from pathogens for which vaccination is currently not possible. The present invention relates to a composition comprising a particle displaying an antigenic component, wherein said composition comprises an antigenic component comprising a first peptide tag and a moiety comprising a second peptide tag, wherein said antigenic component and said moiety are linked by an isopeptide bond between said first and second peptide tags, and wherein said antigenic component is more than 50kDa, or alternatively is a multimer.)

1. A composition comprising particles displaying an antigenic component, wherein the composition comprises:

i) an antigenic component comprising a first peptide tag, and

ii) a moiety comprising a second peptide tag,

wherein the antigenic component and the moiety are linked by an isopeptide bond between the first peptide tag and the second peptide tag, and wherein the antigenic component is greater than 50 kDa.

2. The composition of claim 1, wherein the antigenic component is greater than 60kDa, 70kDa, 80kDa, 90kDa, 100kDa, 110kDa, 120kDa, 130kDa, 140kDa, 150kDa, 160kDa, 170kDa, 180kDa, 190kDa, 200kDa, 300kDa, or 400 kDa.

3. The composition of claim 1 or claim 2, wherein the antigenic component is a monomer or a multimer, optionally wherein the multimer is a trimer, tetramer, pentamer, hexamer, heptamer, octamer, nonamer, or decamer.

4. The composition of any preceding claim, wherein the moiety is a virus, a bacterium, a multimerizing scaffold, a protein component that multimerizes to form virus-like particles (VLPs), a viral structural protein, a multimerizing domain that forms nanoparticles, synthetic nanoparticles, or synthetic VLPs for vaccination.

5. A composition according to any preceding claim, wherein the fraction is HBsAg.

6. The composition of any preceding claim, wherein the first and second peptide tags are selected from any one of the following: a SpyTag and SpyCatcher pair, a SnoopTag or SnoopTag jr and snoopcaptcher pair, a RrgATag, RrgATag2 or a DogTag and rrgcatcher pair, an IsopepTag Pilin-C pair, an IsopepTag-N and Pilin-N pair, a PsCsTag and pscscatccher pair, and a SnoopTag jr and DogTag pair mediated by SnoopLigase or variants, derivatives or modifications thereof, optionally wherein the first peptide tag and the second peptide tag are a SpyTag/SpyCatcher pair.

7. The composition of any preceding claim, wherein the antigenic component comprises an immunogenic component of HCMV pentamers.

8. The composition of claim 7, wherein the immunogenic component of the HCMV pentamer comprises one or more of gH, gL, pUL128, pUL130, or pUL131 subunits.

9. The composition of claim 8, wherein the immunogenic component of the HCMV pentamer comprises all of the gH, gL, pUL128, pUL130, and pUL131 subunits.

10. The composition of any one of claims 7-9, wherein the immunogenic component of the HCMV pentamer comprises a gH subunit with a truncated transmembrane domain.

11. The composition according to any one of claims 8 to 10, wherein the gH, gL, pUL128, pUL130 and pUL131 subunits have the amino acid sequences set forth in SEQ ID NOs 28, 31, 35, 33 and 36, respectively, or functional equivalents thereof.

12. The composition of any one of claims 8 to 11, wherein the first peptide tag is attached to the gH subunit, preferably to the C-terminus of the gH subunit.

13. The composition of any one of claims 1-6, wherein the antigenic component comprises an immunogenic component of RSV-F protein.

14. The composition of claim 13, wherein the immunogenic component of the RSV-F protein is a pre-fusion F protein, optionally a stabilized pre-fusion F protein.

15. The composition of claim 14, wherein the immunogenic component of the RSV pre-fusion F protein comprises F₁Subunit and F₂A trimer of subunits.

16. The composition of any one of claims 13-15, wherein the RSV-F protein has a sequence set forth in any one of SEQ ID NOs 50-58.

17. The composition of claims 13-16, wherein the first peptide tag is attached to the C-terminus of the pre-fusion F protein.

18. The composition of any preceding claim, wherein the first peptide tag is a SpyTag.

19. The composition of claim 18, wherein the SpyTag has the amino acid sequence set forth in SEQ ID No. 30.

20. The composition of claim 18 or claim 19, wherein the SpyTag is attached by a linker.

21. The composition of claim 20, wherein the linker has the amino acid sequence set forth in SEQ ID No. 29.

22. The composition of any preceding claim, wherein the moiety is HBsAg, and optionally wherein the second peptide tag is SpyCatcher.

23. The composition of claim 22, wherein the second peptide is SpyCatcher having the amino acid sequence set forth in SEQ ID No. 38.

24. The composition of claim 22 or claim 23, wherein the moiety is attached to the SpyCatcher by a linker, preferably a flexible linker.

25. The composition of claim 24, wherein the linker has the amino acid sequence set forth in SEQ ID NO 39.

26. The composition of any preceding claim, wherein the composition is an immunogenic composition or a vaccine composition.

27. A vaccine comprising the composition according to any one of claims 1 to 26 for the prevention and/or treatment of a disease.

28. A method of producing a composition according to any one of claims 1 to 26, the method comprising:

-introducing a first nucleic acid encoding a first genetic fusion of a first protein and a first peptide tag into a first host cell;

-incubating the first host cell under conditions to express the first genetic fusion;

-introducing a second nucleic acid encoding a second genetic fusion of a second protein and a second peptide tag into a second host cell;

-incubating the second host cell under conditions to express the second genetic fusion;

-optionally purifying the expressed fraction;

-incubating the expressed components under conditions to form an isopeptide bond between the first and second peptide tags; and optionally purifying the resulting composition.

29. The method of claim 28, wherein the first protein comprises an antigenic component and the second protein comprises a moiety.

30. A nucleic acid molecule for use in a method according to claim 28 or claim 29, wherein the nucleic acid molecule encodes an amino acid sequence set forth in any one of SEQ ID NOs 27 to 41.

31. The nucleic acid molecule of claim 30 having a nucleotide sequence set forth in any one of SEQ ID NOs 12 to 26 or 42 to 46.

32. A nucleic acid molecule for use in a method according to claim 28 or claim 29, wherein the nucleic acid molecule encodes an amino acid sequence set forth in any one of SEQ ID NOs 50 to 58.

33. The nucleic acid molecule of claim 32 having a nucleotide sequence set forth in any one of SEQ ID NOs 47-55.

34. A vector comprising the nucleic acid molecule of any one of claims 30 to 33.

35. A host cell comprising the nucleic acid molecule of any one of claims 30 to 32 or the vector of claim 34.

36. A kit comprising a composition comprising a first immunogenic composition and optionally one or more boosting compositions comprising a second immunogenic composition, wherein the first and/or second immunogenic composition comprises a composition according to any one of claims 1 to 26.

37. A vaccine for the prevention and/or treatment of HCMV infection, comprising a composition according to any one of claims 7 to 12.

38. A vaccine for the prevention and/or treatment of RSV infection, comprising a composition according to any of claims 13 to 17.

Background

Vaccines are a safe and effective way to combat and eradicate infectious diseases. Vaccine development has been very successful, but there is still a range of disease challenges for which there is currently no vaccine available, including many important pathogens that represent daunting immune disorders. It is generally accepted that an effective vaccine must be transported to the lymph nodes for a sufficient period of time to generate an immune response.

Vaccine development has shifted from the use of attenuated or dead pathogens to the use of smaller antigenic components of these pathogens with the aim of generating the required protective immune response while avoiding the risks inherent in the use of such attenuated strains. Efforts have focused on attempting to express immunogenic portions of pathogen components (such as those components required by the pathogen to infect cells), which are limited to short/small peptides and proteins for simplicity due to the technical problem of expressing large or multi-component antigens. However, one potential problem with the use of very short or small peptides is the risk of antigenically variable pathogens that escape the vaccination-induced immune response through changes in the specific part of the antigen.

Expression of large/multi-component antigens will bring immunological advantages, including the ability to allow the production of antibodies against multiple neutralizing epitopes of a pathogen. However, the expression of one or more large antigens that can form a complex (in such a way that the relevant antigenic epitopes are maintained and presented to generate an effective antibody response) remains a great challenge. Thus, there remains a need for improved methods of expressing large antigens and/or multi-component antigens such that they are capable of eliciting clinically significant immune responses.

Previous recombinant vaccines were designed to elicit immune responses against multiple antigenic components, which relied on each component being expressed and packaged separately into distinct particles, for example in the case of the anti-HPV vaccines Cervarix and Gardasil, where the recombinant major capsid L1 proteins of a particular HPV strain are expressed and assembled separately into virus-like particles (VLPs), and then different types of VLPs are combined into vaccine formulations. Alternatively, multiple short epitopes are selected and combined into a single recombinant vaccine (e.g., a multimeric-001 influenza vaccine), but these epitopes are inherently short linear peptides, selected to avoid the manufacturing complexities involved in three-dimensional structure or refolding, and thus are not intended to represent the natural pathogen presented to the immune system in an active infection.

For example, β -herpes human cytomegalovirus (HCMV, also known as human herpesvirus-5 (HHV-5)) is the major viral cause of neonatal developmental disorders. This ubiquitous virus has infected more than 60% of the general population, with initial infection usually being only mild or asymptomatic. After infection, the virus remains latent in the body, but can cause severe disease in people with low immune function (i.e., HIV patients, transplant patients, and people receiving chemotherapy) or the elderly. HCMV is the leading infectious cause of birth defects in developed countries. Infants up to 4/200 were born with HCMV due to congenital infection, and up to 10% of these infants will suffer long-term consequences. HCMV infection is also associated with adult hypertension and atherosclerosis (Cheng et al (May 2009). Fr h K, ed. "cytopmegavirus infection a computers of economic blood pressure". PLoS Patholog.5 (5): e 1000427). Thus, HCMV is a public health priority. However, despite much effort, no successful HCMV vaccine has been developed to date.

Respiratory Syncytial Virus (RSV) is another ubiquitous virus that causes minimal discomfort in healthy adults and older children infected therewith. However, it is the second leading cause of death worldwide in infants under one year of age, second only to malaria. It is estimated that this virus causes 16 million deaths worldwide each year. This virus causes serious respiratory infections and complications, including pneumonia and bronchiolitis. High risk populations include infants under one year of age and immunocompromised patients, the elderly, and people with cardiopulmonary conditions. Also, despite many years of active research and development, no licensed RSV vaccine is currently available.

For diseases such as those caused by RSV and HCMV, there is currently no vaccine available and often current vaccine production methods do not show the desired efficacy, indicating that there is a substantial unmet need to provide alternative types of vaccines to address diseases with such catastrophic consequences.

Several genetically encoded systems capable of spontaneous or assisted amide bond formation have been described recently. For example, SpyTag is a peptide that has been engineered such that when mixed with its protein partner, SpyCatcher, the two components spontaneously and irreversibly form an isopeptide bond. The position of the SpyTag and SpyCatcher components in the protein chain can be engineered at different positions and are reactive under a wide range of pH, buffer and temperature conditions. The SpyTag/SpyCatcher pair and variants and derivatives thereof have been used in vaccine development, but so far only for the presentation of simple antigens. Other genetic coding systems capable of spontaneously forming amide bonds include snoeptag/snoeptagjr and snopopcather; RrgATag/RrgATag2/DogTag and RrgACatcher, IsopepTag/IsopepTag-N and Pilin-C or Pilin-N, PsCsTag and PsCscatcher and SnoopTagJr and DogTag (mediated by Snoop Ligase) and variants of all these systems.

The present inventors have shown that the use of a genetic coding system capable of forming amide bonds enables the use of large/multicomponent antigens in vaccine compositions, which may improve the response to large/multicomponent antigens. This is a surprising result.

Summary of The Invention

In a first aspect of the invention, there is provided a composition comprising particles displaying a protein component, wherein the composition comprises:

i) a protein component comprising a first peptide tag, and

ii) a moiety comprising a second peptide tag,

wherein the protein component and said moiety are linked by an isopeptide bond between said first peptide tag and said second peptide tag, and wherein the protein component is in excess of 50 kDa.

In another aspect of the present invention, there is provided a composition comprising particles displaying a protein component, wherein said composition comprises:

i) a protein component comprising a first peptide tag, and

ii) a moiety comprising a second peptide tag,

wherein the protein component and the moiety are linked by an isopeptide bond between the first peptide tag and the second peptide tag, and wherein the protein component is a multimer.

The protein component may have any function, e.g. it may be or have the properties of an enzyme. The protein component may be a full-length protein, or it may be a portion, segment, domain, or truncation (truncation) of a full-length protein. The protein component may be an antigen or an immunogen. The protein component may also be referred to as an antigenic component.

In another aspect of the present invention, there is provided a composition comprising particles displaying an antigenic component, wherein said composition comprises:

i) an antigenic component comprising a first peptide tag, and

ii) a moiety comprising a second peptide tag,

wherein the antigenic component and said moiety are linked by an isopeptide bond between said first peptide tag and said second peptide tag, and wherein the antigenic component is greater than about 50 kDa.

In some embodiments of any aspect of the invention, the protein component or antigenic component may be more than 60kDa, 70kDa, 80kDa, 90kDa, 100kDa, 110kDa, 120kDa, 130kDa, 140kDa, 150kDa, 160kDa, 170kDa, 180kDa, 190kDa or greater, such as more than 200kDa, more than 300kDa or more than 400 kDa.

The multimer may comprise any number of subunits, which may or may not be covalently linked in the protein component or antigen component. Multimers may comprise 2-20 subunits, alternatively 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more subunits. Alternatively, the multimer may be a dimer, trimer, tetramer, pentamer, hexamer, heptamer, octamer, nonamer, or decamer. The multimer may be from any suitable pathogen, but is preferably a viral multimer.

Non-limiting examples of large protein fractions, i.e., greater than 50kDa or as described above, include Pentameric Complex (PC) and gB glycoproteins from Human Cytomegalovirus (HCMV), G and F glycoproteins from RSV, Hemagglutinin (HA) and Neuraminidase (NA) antigens from influenza a virus, Plasmodium falciparum (Plasmodium falciparum) Pfs230, Plasmodium falciparum CSP, human HER2 receptor, PCSK9, VAR2CSA, Plasmodium falciparum RIPR, Varicella Zoster Virus (VZV) glycoprotein E, rabies virus glycoproteins, and Epstein-Barr virus (EBV) gH/gL complexes.

In some embodiments of any aspect of the invention, the protein component or antigenic component may be a monomer or multimer, such as a dimer, trimer, tetramer or pentamer. In some embodiments of any aspect of the invention, the protein component or antigenic component may be a protein or peptide complex.

Non-limiting examples of multimeric antigen components include Pentameric Complexes (PC) and gB trimers from Human Cytomegalovirus (HCMV), G and F glycoproteins from RSV, Hemagglutinin (HA) antigens and Neuraminidase (NA) antigens from influenza a viruses, some of which are described herein. Other examples include components of pathogens such as viruses, bacteria, fungal pathogens, parasites, or other disease vectors. Suitable multimeric antigenic components include, for example, multimeric antigenic components derived from viruses such as influenza viruses, such as influenza Hemagglutinin (HA) (e.g., influenza trimer), Respiratory Syncytial Virus (RSV), and the like.

The protein component may be attached to the first peptide tag by genetic fusion and recombinantly expressed in a suitable cell. For components that include post-translational modifications such as glycosylation, it may be preferred to express the recombinant protein in eukaryotic or mammalian cell lines.

In one embodiment, a "moiety" is a component on which a protein component or an antigen component may be displayed, for example, such that it is available to the immune system. In one embodiment, the moiety multimerizes to form the particle. Suitably, such moieties may be viruses, bacteria, multimerised scaffolds or protein components multimerised to form VLPs (virus-like particles) for vaccination. Suitably, the moiety may be a component of a bacteriophage, a tobacco mosaic virus particle, an adeno-associated virus-like particle (AAVLP), escherichia coli (e. In one embodiment, the moiety itself is a component of a virus, bacterium, or the like, such that multimerization (e.g., self-assembly) of the moiety forms a particle for displaying a protein component or an antigen component. In one embodiment, the moiety may be a viral structural protein, such as a viral envelope or capsid protein or a surface antigen. Examples of structural proteins include the matrix M1 protein and the viral envelope M2 protein from influenza virus, HBsAg from hepatitis B virus, E.coli phage AP205 virus coat protein (CP3), hemagglutinin-neuraminidase from a variety of viruses, including mumps, and the like. Suitable viral structural proteins will be known to those skilled in the art. In other embodiments, the moiety may be a protein or peptide, such as a multimerization domain forming a nanoparticle, such as IMX313, or a computationally derived particle, such as MI 3. In further embodiments, the moiety may be a synthetic nanoparticle or a synthetic VLP, such as a gold, lipopeptide or poly (lactic-co-glycolic acid) (PLGA) nanoparticle. Other suitable moieties may include liposomes or outer membrane vesicles. Suitably, the moiety comprising the second peptide tag is the moiety to which the second peptide tag is attached.

It may be preferred to use a structural surface antigen from a virus. Thus, the second peptide tag is attached to a structural surface antigen, allowing the formation of a virus-like particle (VLP), the second peptide tag is attached to a virus-like particle, or the second peptide tag is displayed on a virus-like particle. VLPs are non-infectious, self-assembling nanoparticles, and their repetitive, molecularly defined structure is attractive for engineering multivalent, particularly vaccination. VLPs have been produced from components of a number of virus families including hepatitis b virus (including hepatitis b small surface antigen (HBsAg)), Parvoviridae (partoviridae) (e.g. adeno-associated virus), Retroviridae (Retroviridae) (e.g. HIV), Flaviviridae (e.g. hepatitis c virus) and bacteriophages (e.g. Q β, AP 205). Any of these may be suitable for use as part of the present invention.

The second peptide tag may be attached to the moiety by genetic fusion. Such genetic fusions may be at any suitable point in the sequence and are not limited to only the ends. One skilled in the art will appreciate that the fusion protein can be recombinantly expressed in a suitable cell.

Alternatively, the second peptide tag may be displayed on or attached to the moiety by means of chemical conjugation. This would require, for example, the presence of a reactive amine group to allow conjugation to occur.

Accordingly, in one embodiment, the moiety is a surface antigen of hepatitis b virus (HBsAg). Suitably, the HBsAg has the amino acid sequence set out in SEQ ID NO:41 (or a functional equivalent thereof) as described herein.

In one embodiment of any aspect of the invention, the protein component or antigen component is an immunogenic component of HCMV pentamer. Suitably, an antigenic or immunogenic component is a component which is capable of generating an immune response, such as an antibody response against the component, when introduced into a subject, such as a patient. Accordingly, for example, an "immunogenic component of an HCMV pentamer" is a component capable of producing an anti-HCMV antibody response in a subject. Suitably, the immunogenic component comprises one or more (at least one) HCMV pentamer subunit components selected from gH, gL, pUL128, pUL130 and pUL131 (also known as pUL 131A). In some embodiments, the immunogenic component comprises one or more of those "pUL" components or "UL" components. In other embodiments, the immunogenic component comprises one or more of those gH components or gL components. In one embodiment, the immunogenic component comprises a combination of one or more "UL" components and one or more components selected from the gH component or the gL component. In another embodiment of the invention, the immunogenic component of the HCMV pentamer is an HCMV pentamer comprising all of the gH/gL/pUL128/pUL130/pUL131 subunits. Suitably, the gH/gL/pUL128/pUL130/pUL131 subunits have amino acid sequences corresponding to those derived from any known HCMV strain, including laboratory strains and/or clinical isolates, including Towne (GI:239909366), AD169(GI:219879600), Toledo (GI:290564358) and Merlin (GI:155573956) or functional equivalents thereof. Functional equivalents mean amino acid sequences which share some homology and differ only in some amino acids, but retain the functional properties of being able to form antigenic subunits or pentamers which provide protective antibodies. Suitable variants of the components gH/gL/pUL128/pUL130/pUL131A are described, for example, in WO2014/005959 (see pages 4 to 10), hereby incorporated by reference. Advantageously, the use of HCMV pentamer subunits in vaccine approaches can provide immunogenic protection against infection from a wide range of HCMV strains due to the high degree of homology between strains at the level of the pentamer amino acid sequence.

In some embodiments, the antigenic component may correspond to a component of the pathogen or carrier, or a portion thereof. For example, for ease of preparation, the antigenic component may lack a transmembrane domain. Suitably, in an HCMV pentamer, for example, the immunogenic component of the HCMV pentamer comprises a gH subunit having a truncated transmembrane domain (truncated by deletion of one or more amino acids from this region) such that the subunit is secreted into the cell supernatant during production of the protein in the host cell, to facilitate purification.

In one embodiment, the gH/gL/pUL128/pUL130/pUL131A subunits have the amino acid sequences set forth in SEQ ID NOs: 28, 31, 35, 33, 36, respectively (or functional equivalents thereof) (with or without the signal peptide shown). Functional equivalents mean amino acid sequences which share some homology and differ only in some amino acids, but retain functional properties such as the ability to form antigenic subunits or pentamers which provide protective antibodies. In some embodiments, functional equivalents may share 70%, 80%, 90% or more homology with the relevant amino acid sequences. In another embodiment, the gH/gL/pUL128/pUL130/pUL131A subunit is encoded by a nucleic acid sequence such as those set forth in SEQ ID NOS: 13, 16, 20, 18, 21 or a codon-optimized form thereof (with or without a coding sequence for a signal peptide). In some embodiments, any of the gH/gL/pUL128/pUL130/pUL131A subunits may have a signal peptide, e.g., a signal peptide present on a native protein of the strain, a functional equivalent of the signal peptide, or a signal peptide derived from a different HCMV strain. In some embodiments, any one of the gH/gL/pUL128/pUL130/pUL131A subunits may have a signal peptide derived from a heterologous protein. The choice of signal peptide can be determined in order to target the expressed protein to a particular cellular (or extracellular) location, or to confer other functions. After subunit expression, the signal peptide can be cleaved enzymatically (e.g., by a signal peptidase) by natural cellular mechanisms in the expression system used or in vitro. In some embodiments, any one of the gH/gL/pUL128/pUL130/pUL131A subunits may be expressed without a signal peptide. In some embodiments, native sequences, including introns, may be used, wherein these sequences may result in higher expression levels. Suitably, the native nucleic acid sequence of UL128 comprises 2 introns. In another embodiment, the nucleic acid sequence of UL131A comprises an intron. In some embodiments, the intron can be removed. In some embodiments, the native sequence may be codon optimized for the relevant expression system.

In one embodiment of any aspect of the invention, the protein component or antigenic component is an immunogenic component of the RSV virus, such as an attachment glycoprotein (G protein) or a fusion glycoprotein (F protein), both of which control the initial stage of infection. G is a highly glycosylated 90kDa type II integral membrane protein and can mediate viral attachment to the host cell membrane by interacting with heparan sulfate on proteoglycans, and is a good candidate for protein components.

The F protein is an integral membrane protein and consists of three kinds of F₀Monomer composition, processed to F during assembly₁And F₂Subunits, which are covalently linked by two disulfide bonds. The F protein is highly conserved among RSV isolates of subgroup a and subgroup B, and the amino acid sequences show 90% or greater identity. F is a 574 amino acid class I fusion protein consisting of a 50 kilodalton (kDa) carboxy-terminal F1 fragment and a 20kDa amino-terminal F2 fragment; making it a trimer of heterodimers. Characterized by two furin cleavage sites that release a 27 amino acid glycopeptide and expose a hydrophobic fusion peptide at the amino terminus of F1. There are two N-linked glycosylation sites in F2, and only one in F1. After removal of the 25 amino acid signal peptide and 27 amino acid glycopeptide between F2 and F1, the remaining F ectodomain consists of 472 amino acids. There are only 25 amino acids in the F ectodomain that differ between subtype a and subtype B.

To develop antigenic compositions from RSV-F protein, some studies have focused on variants of the pre-fusion protein that are prepared as trimers. Variants were generated by genetically fusing the two subunits of mature pre-F into a single chain. A DS-Cav1 variant has been prepared in which F2 is genetically fused to F1 and the fusion peptide and pep27 region are deleted. The difference in linkers between the F2 and F1 subunits appears to affect immunogenicity, and thus variants may use a selection of different linkers. The native RSV-F protein sequence can be found at accession P03420.1.

Several forms of pre-fusion F proteins have been studied and developed and subsequently published. These prefusion trimers are all suitable for use in the present invention. The DS-Cav1 stabilized fusion glycoprotein is derived from natural protein. EP2222710, incorporated herein by reference, also discloses recombinant RSV antigens comprising a soluble F protein polypeptide comprising the F2 and F1 domains of an RSV-F protein polypeptide and a trimerization domain. At nat.commun.2015; 6:8143, Krarup et al, describes a highly stable pre-fusion RSV-F protein, again incorporated by reference.

WO2014/160463, incorporated herein by reference, describes isolated recombinant RSV-F proteins stabilized in a pre-fusion conformation and nucleic acid molecules encoding the recombinant RSV-F proteins.

WO2017/172890, incorporated herein by reference, describes substitution-modified pre-fusion RSV-F proteins and nucleic acids encoding same. In Nat Struct Mol biol.2016sep, also incorporated herein by reference; 23(9) 811-820, Iterative structural-based improvement of a respiratory synthetic virus fusion, M.Gordon Joyce, Baoshan Zhang, Li Ou, Man Chen, Gwo-Yu Chuang, Aliakscand drive, Wing-Pu Kong, Yen-Ting Lai, Emily J.Rundlet, Yaroslav yvsky, Yongping Yang, Ivelin S.Georgiev, Miklos Gutman, Christopher R.Lees, ie Parra, Malika Satry, Cinlque to, Guillme Some B.E.Stewart-Jowart, Paphyas V.John, Jollha K.John, John K.K.K.K..

Encoding recombinant F linked to T4 Fibritin trimerization domain₂-F₁Exemplary nucleic acid sequences for ectodomain protomers (protomers) can be obtained under the following accession numbers: LP884611.1, LP884610.1, LP884609.1 and LP 884608.1.

In some embodiments, the protein component or antigenic component may correspond to a component of the pathogen or carrier or a portion thereof. For example, for ease of preparation, the antigenic component may lack a transmembrane domain. Suitably, in the RSV-F protein or pre-fusion conformation thereof, for example, the immunogenic component of the F protein comprises F having a truncated transmembrane domain (truncated by deletion of one or more amino acids from this region)₂-F₁A subunit such that the subunit is secreted into the cell supernatant during production of the protein in the host cell to facilitate purification. Thus, the RSV-F protein lacks a functional TM domain. Optionally, with a first peptide tagThe genetic fusion of (a) does prevent the F protein from residing in the membrane despite the presence of a functional transmembrane domain.

In one embodiment, the prefusion stabilized subunits have the amino acid sequences set forth in SEQ ID NOS: 50-58, respectively (or functional equivalents thereof). Functional equivalents mean amino acid sequences which share some homology and differ only in some amino acids, but retain functional properties such as the ability to form antigenic subunits which provide protective antibodies. In some embodiments, functional equivalents may share 70%, 80%, 90% or more homology with the relevant amino acid sequences. In one embodiment, the prefusion-stabilized RSV-F trimer may not comprise a heterologous trimerization domain.

The protein component comprises a first peptide tag. The first peptide tag may be attached to the protein component by expression of a recombinant fusion protein. The skilled person will be aware of techniques for genetically fusing peptide sequences in order to express recombinant proteins in a suitable cell system. For moieties that include post-translational modifications such as glycosylation, it is preferred to express the recombinant protein in eukaryotic or mammalian cell lines.

Advantageously, the use of first and second peptide tags forming isopeptide bonds, such as the SpyTag-spycatccher system described herein, allows for the "decoration" of large antigens and/or multimeric antigens, such as HCMV pentamers or immunogenic components thereof, in the correct formation and orientation to the portion displaying the large antigen, such as VLPs, such that the antigen is presented to the immune system in a manner that enables the production of anti-antigen (e.g., anti-HCMV) antibodies that are capable of providing a protective/neutralizing/immunogenic effect. Traditional vaccination methods using soluble antigens (even large antigens such as multimers/pentamers) may be less effective in producing protective/neutralizing/immunogenic effects. Advantageously, the display of antigens (e.g. multimeric antigens) on particles such as VLPs or nanoparticles results in the presentation of geometrically repeating arrays of the same antigens, which arrays are capable of robustly triggering immune responses compared to soluble antigens. VLPs or other suitable particles of larger size may also have a greater immunogenic effect than "free" antigens. Furthermore, the display orientation of multimeric antigens such as HCMV pentamers may be important for immunogenicity. Pairs of tags, such as the SpyTag-SpyCatcher system described herein, are used to attach multimeric antigens to particles, allowing the antigens to be attached to the particles in a particularly advantageous orientation. For example, in the case of HCMV, the gH/gL subunit has a lower probability of neutralizing epitopes than the "UL" subunit. Thus, advantageously, the present invention allows to determine the display direction of HCMV pentamers on the particle by suitable localization of the first peptide tag, such that e.g. the "UL" subunit is displayed towards the outside of the particle and is thus more readily accessible to the immune system of the individual. Alternatively/additionally, the localization of the first peptide tag on the antigen can be determined so as to generate an antigenic orientation similar to that on the native virus, thereby presenting the particles displaying the antigen to the immune system in an orientation more likely to induce an immune response to the invading live virus.

In contrast, traditional methods of presenting proteins on VLPs may involve chemical ligation, a disadvantage of chemical ligation being that such chemical reactions may be more random, such that the correct (e.g., immunologically preferred) antigenic orientation cannot be obtained with certainty, and may represent only a small fraction of the ligation reactions obtained. Furthermore, the processes involved in chemical conjugation may make it unlikely that the 3-D structure required for proper antigen presentation will be maintained. For example, in Brune et al 2016; some of the disadvantages of the conventional methods are described in Scientific Reports,6:19234, DOI:10.1038/srep19234, Brune et al Bioconjugate Chemistry,2017,28, 1544-.

Similarly, genetic fusion of antigens to viral coat proteins has proven challenging and time consuming because of the problems of misfolding and determining conditions for optimal expression of the two components. Furthermore, genetic fusions are not suitable for expression of large antigens or multicomponent antigens, since efficient expression in the correct conformation is too difficult to achieve.

In order to present the protein or antigen component in a manner that is immunogenic, the position of the first peptide tag needs to be carefully designed so that the native protein conformation is maintained, and optionally any post-translational modifications, if appropriate. For some antigens, the retention of glycosylation does not affect the way epitopes are presented, but for other antigens, retention or removal of glycosylation improves efficacy. For the protein component that is a transmembrane protein, the transmembrane portion of the protein component provides a good target for the localization of the first peptide tag, since, for example, the sequence is not involved in the conformation of the protein component that is antigenic, and the provided effect is no longer required in a vaccine. If the protein component does not comprise a transmembrane protein, it may prove advantageous to fuse the first peptide tag to the C-terminus or N-terminus of the component or subunit thereof (in the case of multimers), but the first peptide tag may also be comprised in any part of the sequence. Alternatively, the first peptide tag may be positioned in a loop on the protein component or the antigenic component.

In order to present immunogenic components, such as those of the HCMV pentamer, the position of the first peptide tag needs to be carefully designed so that the native protein conformation is maintained. For HCMV, in one embodiment, the attachment is through the gH subunit, suitably through the C-terminus of the gH subunit, or the transmembrane domain of the gH subunit (or a portion thereof). In addition to maintaining the conformation of the pentamer (or components of the pentamer), this rational design also allows the target region of the pentamer to be presented toward the exterior of the particle as discussed above. As used herein, a target region is a portion of a protein known to elicit antibodies with neutralizing effects, and may also be referred to as an immunogenic portion.

In order to present an immunogenic component, such as that of the RSV pre-fusion F protein, careful design of the position of the first peptide tag is required so that the native protein conformation is maintained. For the RSV-F pre-fusion protein, in one embodiment, a suitable attachment is through the C-terminus of the F pre-fusion protein, through the 3' terminus of the nucleic acid encoding it. In addition to maintaining the conformation of the pre-fusion F protein (or components thereof), this rational design also allows for the presentation of most of the neutralizing epitopes of the pre-fusion F protein towards the exterior of the particle as discussed above. The same considerations apply to any other variant of the F protein. The inventors have shown that it is effective to include a first peptide tag at the C-terminus, leaving the immunogenic protein component to fold correctly.

In one embodiment, the first and second peptide tags are part of a peptide tag/binding partner pair capable of forming an isopeptide bond. The isopeptide bond may be spontaneous, i.e., without assistance, or require assistance, i.e., assistance from a ligase or other adjunct. Suitably, the first and second peptide tags are a SpyTag/SpyCatcher pair. Suitably, the first and second peptide tags are selected from the list comprising: SpyTag/SpyCatcher, SnoopTag/SnoopTagJr and SnooppCatcher, RrgATag/RrgATag2/DogTag and RrgACatcher, IsopepTag/IsopepTag-N and Pilin-C or Pilin-N, PsCsTag and PsCspcatcher, and SnoopTag Jr and DogTag (mediated by SnoopLigase) and variants, derivatives and modifications of all these systems.

Suitably, the first peptide tag is a peptide tag from a peptide tag/binding partner pair, such as SpyTag, and the second peptide tag is a binding partner, such as SpyCatcher. In another embodiment, the first peptide tag is a binding partner, such as SpyCatcher, and the second peptide tag is a peptide tag component from a peptide tag/binding partner pair, such as SpyTag. Suitably, the first peptide tag is a peptide tag from a peptide tag/binding partner pair, such as a snooppag, and the second peptide tag is a binding partner, such as a snooppcher. In another embodiment, the first peptide tag is a binding partner, such as a snooppcher, and the second peptide tag is a peptide tag component from a peptide tag/binding partner pair, such as a snooppag. As can be seen, the first peptide tag may be a "tag" or a "catcher"; the second peptide tag is a partner of this pair, being the "catcher" or "tag", respectively. Suitable peptide tag/binding partner pairs are described in detail in WO2011/09877, WO2016/193746, WO2018/18951 and WO2018/197854, incorporated herein by reference.

In one embodiment, the protein component or antigen component is attached to any one of SpyTag, snoeptag, RrgATag2, DogTag, IsopepTag-N, PsCsTag, and snoeptag jr as a first peptide tag.

If desired, the first peptide tag may be attached by a linker, which may be rigid or flexible. Those skilled in the art will recognize which linkers are suitable.

In another embodiment, the moiety is attached to any of SpyCatcher, SnoopCatcher, RrgACatccher, Pilin-C, Pilin-N, PsCsCatcher, and DogTag (mediated by SnoopLigase) as a second peptide tag.

As previously discussed, the moiety may be any suitable moiety, including a synthetic multimerization platform.

The second peptide tag may be attached to any suitable position of the moiety that does not affect the ability of the moiety to fold and form a suitable conformation. Genetic fusion may be preferred. It may be preferred to include the second peptide tag at the C-terminus or N-terminus of the moiety, but the second peptide tag may also be included in any portion of the sequence. Alternatively, a second peptide tag may be positioned in a loop on the moiety. For example, genetic fusion of SpyCatcher to the N-terminus of the viral coat protein (CP3) of RNA phage AP205 is described in Brune et al, Scientific Reports volume 6, articule number:19234 (2016). Alternative fusions using self-assembling synthetic proteins as multimerization platforms are discussed in Bruun et al, ACS Nano,2018,12(9), pp 8855-. Alternatively, the second peptide tag may be attached by chemical conjugation.

If desired, the second peptide tag may be attached by a linker, which may be rigid or flexible. Those skilled in the art will recognize which linkers are suitable.

In one embodiment, an antigenic component, such as HCMV pentamer or an immunogenic component thereof, is attached to SpyTag. One suitable SpyTag has the amino acid sequence set forth in SEQ ID No. 30.

The SpyTag may be attached by a linker. Suitable linkers include those having the amino acid sequences set forth in SEQ ID NO. 29.

In another embodiment, the moiety is attached to a SpyCatcher binding partner (second peptide tag). The moiety may suitably be an HBsAg. One suitable SpyCatcher has the amino acid sequence set forth in SEQ ID NO 38. In one embodiment, SpyCatcher is attached by a linker. The linker may be a rigid linker or a flexible linker, suitably wherein the linker has the amino acid sequence set out in SEQ ID No. 39.

In another embodiment, the protein composition or antigenic composition according to any aspect or embodiment of the invention further comprises another (preferably different) protein comprising the first peptide tag.

In another embodiment, the composition according to any aspect or embodiment of the invention further comprises another (preferably different) antigen comprising the first peptide tag, such as another HCMV antigen. Suitably, the other HCMV antigen is glycoprotein B. Suitably, the glycoprotein B sequence is described in, for example, WO2014/005959, see SEQ ID NOs: 21, 22, 23 or 36. In one embodiment, the composition comprises a particle (e.g., VLP) displaying both HCMV pentamer and another HCMV antigen.

In one embodiment, the composition is an immunogenic composition or a vaccine composition. Preferably, the immunogenic composition or vaccine composition is a composition capable of inducing an immune response, such as an antibody response, upon administration to an individual. Suitably, the immune response may be a protective immune response. Suitable immunogenic compositions may further comprise additional components, including adjuvants, immunostimulants and/or pharmaceutically acceptable excipients.

For example, suitable adjuvants may be based on aluminum, peptides, squalene, liposomes, oil-in-water emulsions, and saponins, and may includeMF59, AS01, MatrixM, muramyl dipeptide, and Quil a. Water-in-oil adjuvants are also suitable. Squalene oil-in-water emulsions, such as Addavax^TMAnd is suitable.

Accordingly, in another aspect or embodiment of the invention there is provided an immunogenic or vaccine composition comprising a composition according to the invention. Suitably, the vaccine composition comprises a vaccine dose, which is an amount of the composition according to the invention that provides immunogenicity from the infectious agent/carrier, preferably an immunoprotective effect against the infectious agent/carrier, such as a neutralizing effect against HCMV infection. Suitably, the vaccine composition comprises a vaccine dose, which is the amount of the composition according to the invention that provides a neutralising effect against an infectious agent/vector, such as a neutralising effect against RSV infection. Antibodies, preferably neutralizing antibodies raised against immunogenic compositions, can be detected and measured by methods familiar to those skilled in the art, including, for example, the standardized ELISA assay or the micro-neutralization assay described herein.

In another aspect, there is provided a VLP comprising:

i) moieties comprising a first peptide tag

ii) a protein comprising a second peptide tag

Wherein the first peptide tag and the second peptide tag form an isopeptide bond. In some embodiments, the moiety is HBsAg. However, as previously described, any suitable portion may be used.

Suitably, the first peptide tag is a peptide tag from a peptide tag/binding partner pair, such as SpyTag, and the second peptide tag is a binding partner, such as SpyCatcher. In another embodiment, the first peptide tag is a binding partner, such as SpyCatcher, and the second peptide tag is a peptide tag from a peptide tag/binding partner pair, such as SpyTag. Other suitable peptide tag/binding partner pairs are described herein and will be known to those skilled in the art. Suitably, the first and second peptide tags are selected from the list comprising: SpyTag/SpyCatcher, SnoopTag/SnoopTagJr and SnooppCatcher, RrgATag/RrgATag2/DogTag and RrgACatcher, IsopepTag/IsopepTag-N and Pilin-C or Pilin-N, PsCsTag and PsCspcatcher, and SnoopTag Jr and DogTag (mediated by SnoopLigase) and variants, derivatives and modifications of all these systems.

Suitably, the protein comprising the second peptide tag is a protein or peptide complex of greater than 50 kDa. The protein comprising the second peptide tag may be a protein or peptide complex of greater than 50kDa, 60kDa, 70kDa, 80kDa, 90kDa, 100kDa, 110kDa, 120kDa, 130kDa, 140kDa, 150kDa or 160kDa, 170kDa, 180kDa, 190kDa or greater, such as greater than 200kDa, greater than 300kDa or greater than 400 kDa.

In one embodiment, the protein comprising the second peptide tag is a multimeric protein. In one embodiment, the protein comprising the second peptide tag is an antigen, preferably a multimeric antigen. Suitably, the multimeric antigen may be an HCMV pentamer as described herein. Suitably, the protein may be an RSV-F protein or derivative thereof (such as a pre-fusion F protein). In one embodiment, the protein comprising the second peptide tag is an immunogenic component of HCMV pentamer. The HCMV pentamer as described herein and comprising a suitable linker and tag (gH/gL/pUL128/pUL130/pUL131) has a molecular weight of more than 160 kDa. Other suitable large proteins or antigens or multimeric proteins or antigens include antigens from other infectious agents, including viruses such as influenza, RSV, and the like.

Advantageously, the use of HBsAg as a Vector (VLP) in this manner may also result in a boost against HepB, or what is described as an anti-Hepatitis B Virus (HBV) response.

In another aspect, there is provided a VLP comprising:

i) protein comprising a first peptide tag

ii) a moiety comprising a second peptide tag

Wherein the first peptide tag and the second peptide tag form an isopeptide bond. In some embodiments, the moiety is HBsAg. However, as previously described, any suitable portion may be used.

Suitably, the first peptide tag is a peptide tag from a peptide tag/binding partner pair, such as SpyTag, and the second peptide tag is a binding partner, such as SpyCatcher. In another embodiment, the first peptide tag is a binding partner, such as SpyCatcher, and the second peptide tag is a peptide tag from a peptide tag/binding partner pair, such as SpyTag. Other suitable peptide tag/binding partner pairs are described herein and will be known to those skilled in the art. Suitably, the first and second peptide tags are selected from the list comprising: SpyTag/SpyCatcher, SnoopTag/SnoopTagJr and SnooppCatcher, RrgATag/RrgATag2/DogTag and RrgACatcher, IsopepTag/IsopepTag-N and Pilin-C or Pilin-N, PsCsTag and PsCspcatcher, and SnoopTagJr and DogTag (mediated by SnoopLigase) and variants, derivatives and modifications of all these systems.

Suitably, the protein comprising the first peptide tag is a protein or peptide complex of greater than 50 kDa. The protein comprising the first peptide tag may be a protein or peptide complex of greater than 50kDa, 60kDa, 70kDa, 80kDa, 90kDa, 100kDa, 110kDa, 120kDa, 130kDa, 140kDa, 150kDa or 160kDa or greater, in particular 200kDa, 300kDa or even 400kDa or greater. In one embodiment, the protein comprising the first peptide tag is a multimeric protein. In one embodiment, the protein comprising the second peptide tag is an antigen, preferably a multimeric antigen. Suitably, the multimeric antigen may be an HCMV pentamer as described herein. Suitably, the protein may be an RSV-F protein or derivative thereof (such as a pre-fusion F protein). In one embodiment, the protein comprising the first peptide tag is an immunogenic component of HCMV pentamer. The HCMV pentamer as described herein and comprising a suitable linker and tag (gH/gL/pUL128/pUL130/pUL131A) has a molecular weight of more than 160 kDa. Other suitable large proteins or antigens or multimeric proteins or antigens include antigens from other infectious agents, including viruses such as influenza, RSV, and the like.

Advantageously, the use of HBsAg as a carrier (VLP) in this way may also result in an enhancement against HBV.

In another aspect of the invention, HCMV pentamers linked to SpyTag as described herein are provided.

According to another aspect of the present invention there is provided a method of producing a composition or VLP according to the present invention, the method comprising:

-introducing a first nucleic acid encoding a first genetic fusion of a first protein and a first peptide tag into a first host cell;

-incubating the first host cell under conditions to express the first genetic fusion; optionally purifying the expressed fraction;

-introducing a second nucleic acid encoding a second genetic fusion of a second protein and a second peptide tag into a second host cell;

-incubating the second host cell under conditions to express the second genetic fusion; optionally purifying the expressed fraction;

-incubating the expressed component under conditions to form an isopeptide bond between the first peptide tag and the second peptide tag; optionally purifying the resulting composition.

Suitably, the expressed components are incubated together so as to form isopeptide bonds. The formation of isopeptide bonds may require co-incubation with a ligase or the like.

Suitably, the method of producing a composition or VLP according to the invention may be used to produce a composition comprising an antigen component displayed on a VLP.

In some embodiments, when the "immunogenic component of an HCMV pentamer" comprises the entire HCMV pentamer, recombinant production of the HCMV pentamer component requires that each subunit be expressed at the correct stoichiometry to form the pentamer, and be correctly folded for assembly. In these embodiments, it is desirable to exclude from the final product complexes that are only part of the desired pentamer (e.g., gH/gL dimers and tetramers, or tetramers lacking any one of the five subunits). Advantageously, the present invention overcomes the problems that may otherwise be associated with the expression of all vaccine components (i.e. 5 subunits of HBsAg and HCMV pentamers) in one system by providing a simple method of separately preparing the components and then conjugating them. Accordingly, in one embodiment, a purification tag is incorporated into UL130(Hofmann et al, DOI 10.1002/bit 25670). Similar principles will apply to other immunogenic components as well.

In some embodiments, when the "immunogenic component of RSV-F protein" comprises the entire F protein or a derivative thereof, recombinant production of the component of the F protein or derivative thereof requires proper folding thereof for assembly, wherein the derivative comprises a pre-fusion F protein trimer.

Suitably, the method is for producing a composition comprising HCMV pentamers displayed on HBsAg VLPs. Suitably, the method is for producing a composition comprising RSV-F pre-fusion F protein trimers displayed on HBsAg VLPs.

In another aspect of the present invention, a vaccine for preventing and/or treating a disease is provided. Suitably, the vaccine comprises a composition or VLP according to any aspect or embodiment of the invention. In one embodiment, the disease is HCMV infection. In another aspect, prophylactic methods of HCMV treatment are provided. Suitably, the vaccine is for use in humans. Suitably, the vaccine is for an adult, such as a female of child bearing age or pregnant female. In another aspect, the invention provides a method of inducing an immunogenic response (e.g. a protective immune response) against HCMV in an individual, wherein the method comprises administering a composition according to any aspect or embodiment of the invention.

In another aspect of the invention, there is provided a composition according to any aspect of the invention for use as a medicament.

In a further aspect of the invention, there is provided a composition according to any aspect of the invention for use as a vaccine, preferably for use in the prevention and/or treatment of HCMV infection. The composition for use as a medicament or vaccine according to the invention may be administered to an adult, such as a female of child bearing age or a pregnant female.

In another aspect, the invention provides a nucleic acid molecule for use in a method according to the invention. In one embodiment, the nucleic acid molecule according to the invention comprises a nucleic acid sequence encoding an amino acid sequence as set forth in any one of SEQ ID NOs 27 to 41. In one embodiment, the nucleic acid molecule according to the invention comprises the nucleic acid sequence set forth in any one of SEQ ID NOs 12 to 26 or 42 to 46.

In another aspect, the invention provides a plurality of nucleic acid molecules, including those encoding the amino acid sequences set forth in SEQ ID NOs 27 to 41. In one embodiment, the nucleic acid molecules of the invention include those having the sequences set forth in any one of SEQ ID NOs 12 to 26 or 42 to 46.

In another aspect, the invention provides a nucleic acid molecule for use in a method according to the invention. In one embodiment, the nucleic acid molecule according to the invention comprises a nucleic acid sequence encoding an amino acid sequence as set forth in any one of SEQ ID NOs 50 to 58. In one embodiment, the nucleic acid molecule according to the invention comprises the nucleic acid sequence set forth in any one of SEQ ID NOs 47 to 55.

In another aspect, the invention provides a plurality of nucleic acid molecules, including those encoding the amino acid sequences set forth in SEQ ID NOS: 50 to 58. In one embodiment, the nucleic acid molecules according to the invention include those having the sequences listed in any one of SEQ ID NOs 47 to 55.

In another aspect, the invention provides a vector comprising a nucleic acid molecule or a plurality of nucleic acid molecules according to the invention. Suitable vectors are expression vectors for expressing the amino acid sequences of any component of the composition according to the invention.

In another aspect, the invention provides a host cell for expressing a component of a composition according to the invention. Suitable host cells may be those for transient or stable expression of these components. Methods and host cells for expressing CMV proteins are described, for example, in WO2014/005959 and WO2016/067239, both of which are incorporated by reference. In some embodiments, the components may be glycosylated.

In another aspect of the invention, a kit is provided comprising a composition according to the invention for use in a prime-boost vaccination regimen. Suitably, the kit may comprise a priming composition comprising a first immunogenic composition according to the invention and a boosting composition comprising a second immunogenic composition according to the invention. Alternatively, the kit may be provided to provide a single or multiple dose vaccination regimen, i.e. 1, 2,3, 4, 5, 6, 7, 8, 9 or 10 doses. Accordingly, in another aspect, the invention provides a dosage regimen comprising doses administered at intervals of about 3 weeks.

Drawings

FIG. 1 SDS-PAGE and Western blot analysis of purified pentamer-SpyTag under non-reducing and reducing conditions. Lane 1: ColorPlus prestaines a broad range protein ladder, the size indicated in kDa; lane 2: a non-reducing sample; lane 3: and reducing the sample. A) SDS-PAGE and coomassie stain analysis indicated the location of the HCMV pentamer component, the left side of the gel was non-reducing and the right side of the gel was reducing. B) Western blot analysis using anti-HCMV pentamer antibody.

FIG. 2 SDS-PAGE and Western blot analysis of purified SpyCatcher-HBsAg under non-reducing conditions (NR) and reducing conditions (R). A) SDS-PAGE and Coomassie staining. B) Western blot analysis using anti-HBsAg monoclonal antibodies.

FIG. 3: HPLC analysis using a s200 inch 3.2/300 column. A) Mu.l of purified HCMV pentamer-SpyTag was loaded and eluted as a single peak. B) 10 μ l of purified SpyCatcher-HBsAg was loaded and eluted with the column's external water volume as a single major peak.

FIG. 4: SDS-PAGE and Western blot analysis of conjugated pentamer-SpyTag and Spycatcher-HBsAg under reducing conditions. 1: ColorPlus prestaines a broad range protein ladder, the size indicated in kDa; 2: a conjugate; 3: pentamer-SpyTag; 4: SpyCatcher-HBsAg. A) SDS-PAGE and Coomassie staining. B) Western blot using anti-HBsAg monoclonal antibody. C) Western blot using anti-pentamer polyclonal antibody.

FIG. 5: HPLC analysis using a s200 inch 3.2/300 column. Mu.l of conjugated pentamer-SpyTag- -Spycatcher-HBsAg was loaded and eluted with the column external water volume as the main peak.

FIG. 6: HCMV pentamer-HBsAg vaccine contrasts the immunogenicity of the pentamer protein vaccine after a single immunization, using Addavax as an adjuvant. BALB/c mice were immunized with 1. mu.g or 0.1. mu.g of HCMV pentamer-SpyTag as soluble protein or as pentamer-HBsAg VLP. Titers were measured from mouse sera by standardized ELISA. The lines represent mean and the error bars represent standard deviation (n-10). Mice immunized with HCMV pentamer-HBsAg VLPs show a significantly stronger serum IgG antibody response compared to mice immunized with HCMV pentamer protein alone, even when the pentamer equivalent VLP dose is 10x lower.

FIG. 7: compared with the pentameric protein vaccine,neutralization activity of sera from mice immunized with HCMV pentamer-HBsAg vaccine. Addavax was used as an adjuvant for the vaccine and the response after one immunization (post-prime) or after two immunizations (post-boost) was shown. For AD169^wt131Strain (display functional pentamer) infected ARPE-19 cells measured NT₅₀. In the same assay, the neutralizing titers of Cytogam and a commercially available neutralizing anti-gH mAb (HCMV16(51C1), from Bio-Rad Antibodies) are shown.

FIG. 8: without adjuvant, HCMV pentamer-HBsAg vaccine contrasts the immunogenicity of the pentamer protein vaccine after one or two immunizations. BALB/c mice were immunized with 1. mu.g or 0.1. mu.g of HCMV pentamer-SpyTag conjugated to Spycatcher-HBsAg or with 1. mu.g of pentamer-SpyTag protein. Titers were measured from mouse sera by standardized ELISA. The lines represent mean and the error bars represent standard deviation (n-10). Mice immunized with HCMV pentamer-HBsAg VLPs show a significantly stronger serum IgG antibody response compared to mice immunized with HCMV pentamer protein alone, even when the pentamer equivalent VLP dose is 10x lower.

FIG. 9: neutralization activity of serum from mice immunized with HCMV pentamer-HBsAg vaccine compared to pentamer protein vaccine. The vaccine was without adjuvant and showed a response after one immunization (post-prime) or after two immunizations (post-boost). For AD169^wt131NT50 was measured on ARPE-19 cells infected with strain (displaying functional pentamers). In the same assay, the neutralizing titers of Cytogam and a commercially available neutralizing anti-gH mAb (HCMV16(51C1), from Bio-Rad Antibodies) are shown.

FIG. 10 SDS-PAGE and Western blot analysis of purified RSV-F-SpyTag under non-reducing and reducing conditions. A) SDS-PAGE and Coomassie staining analysis, lane 1: ColorPlus prestaines a broad range of protein ladders; lane 2: a non-reducing sample; lane 3: and reducing the sample. B) Western blot analysis using anti-RSV-F monoclonal antibody, lane 1: ColorPlus prestaines a broad range of protein ladders; lane 2: a non-reducing sample; lane 3: and reducing the sample.

FIG. 11 SDS-PAGE and Western blot analysis of Spycatcher-HBsAg conjugated RSV-F-SpyTag under reducing conditions. 1: ColorPlus prestaines a broad range of protein ladders, 2: RSV-F-SpyTag-SpyCatcher-HBsAg conjugate, 3: RSV-F-SpyTag, 4: SpyCatcher-HBsAg. A) SDS-PAGE and Coomassie staining. B) Western blot using anti-HBsAg monoclonal antibody. C) Western blotting using an anti-RSV-F monoclonal antibody.

FIG. 12 immunogenicity of conjugated RSV-F-SpyTag- -Spycatcher-HBsAg ('Sc 9-10-HBsAg') versus unconjugated RSV-F-SpyTag ('Sc 9-10'). In the absence of adjuvant or with Addavax^TMAs adjuvant, BALB/c mice were immunized with 1 μ g of RSV-F-SpyTag conjugated to SpyCatcher-HBsAg (RSV-F VLP) or 1 μ g of RSV-F-SpyTag protein (n ═ 8). The RSV-F antigen is sc9-10DS-Cav 1A 149C Y458C-SpyTag.

Detailed Description

Virus-like particles

Traditionally, vaccine approaches have used attenuated or dead whole pathogens, although this has been replaced by the use of recombinant subunit vaccines containing proteins from the appropriate pathogen. Recently, methods of using virus-like particles (VLPs) have been developed. VLPs are particles whose size (about 20-200nm), shape, and repetitive protein arrangement resemble viruses, but lack any genetic material from a pathogen. Due to their size, VLPs are more likely to flow to lymph nodes, making them ideal for uptake and presentation by antigen presenting cells. In addition, their repetitive structure facilitates complement fixation and B cell receptor cross-linking (Kushnir et al Vaccine 2012; Vol 31(1): 58-83). However, their mechanism of action is not limited to theory.

HCMV

Human cytomegalovirus (HCMV, also known as human herpesvirus-5 (HHV-5)) is a virus that most adults have exposed, and the initial infection is usually only mild or asymptomatic. After infection, the virus remains latent in the body, but can cause severe disease in people with low immune function or the elderly. HCMV is also a major infectious cause of birth defects in developed countries. Infants up to 4/200 were born with HCMV due to congenital infection, and up to 10% of these infants will suffer long-term consequences. HCMV infection is also associated with adult hypertension and atherosclerosis (Cheng et al (May 2009). Fr h K, ed. "cytopmegavirus infection a computers of economic blood pressure". PLoS Patholog.5 (5): e 1000427).

This complex has been identified as a potentially useful vaccine target for HCMV based on the observation that antibodies directed against pentameric complexes of HCMV comprising the viral proteins gH/gL/pUL128/pUL130/pUL131A can neutralize viral entry into epithelial cells and reduce the risk of perinatal spread of HCMV. However, despite much effort, no successful HCMV vaccine has been developed to date.

HCMV pentamer

HCMV strains, including clinical isolates and laboratory strains, differ in their genomic sequence. HCMV strains include Merlin (GI:155573956), Towne (GI239909366) and AD169(GI:219879600), Toledo (GI290564358) and TB 40/E. HCMV contains a variety of membrane proteins and protein complexes. The pentameric protein gH/gL/pUL128/pUL130/pUL131A is important for HCMV infection of epithelial and endothelial cells, which is thought to proceed via the endocytic pathway. Other combinations of components of the complex have been shown to be important for infection of e.g. fibroblasts. "pUL" subunits/components are also referred to as "UL"; "pUL 131" is also referred to as "pUL 131A" and "pUL 131 a" or "UL 131A".

A number of HCMV strains have been deposited with the ATCC and can be found as follows: merlin (VR-1590), Towne (VR-977) and AD169 (VR-538). The genomic sequence can be referenced by the following accession numbers: merlin (AY446894.2), Towne (GO121041.1), AD169(FJ527563.1), Toledo (GU37742.2) and TB40/E (KF 297339.1).

RSV

Respiratory syncytial virus is the leading cause of severe respiratory disease in young children worldwide. It is estimated that 340 million children under 5 years of age are hospitalized annually for severe RSV lower respiratory tract infections, with the highest incidence among children under 6 months of age. Most deaths occur in infants under 1 year of age and in developing countries. Currently, options for prevention and control are limited.

RSV pre-fusion trimers

The F glycoprotein is a type I viral fusion protein. It is considered that RThe SV F precursor (F0) is cleaved by a furin-like protease at two sites, which results in three fragments. Shorter N-terminal fragment (F)₂) Covalently attached to the larger C-terminal fragment by two disulfide bonds (F)₁) Covalently attached. The 27 amino acid fragment in between dissociates after cleavage and is not present in the mature protein.

As previously discussed, there are many stabilized prefusion F trimers available. In the examples submitted herein, exemplary sequences encoding these prefusion trimers can be found in SEQ ID NOs 48, 49, 54 and 55. Sequences comprising fusions to SpyTag are included as SEQ ID NOs 47 and 53. For the prefusion trimers, the amino acid sequences are shown in SEQ ID NO 51, 52, 57 and 58, and for the SpyTag-carrying amino acid sequences are shown in SEQ ID NO 50 and 56. Other exemplary sequences are mentioned herein.

Peptide tag/binding partner pairs

Proteins capable of spontaneously forming isopeptide bonds (so-called "isopeptide proteins") have been advantageously used to develop peptide tag/polypeptide binding partner pairs (i.e., two-part linkers) that covalently bind to each other and provide irreversible interaction (see, e.g., WO2011/098772 and WO2016/193746, both incorporated herein by reference, and WO2018/189517 and WO2018/197854, both incorporated herein by reference). In this regard, a protein capable of spontaneously forming isopeptide bonds may be expressed as separate fragments to give a peptide tag and a polypeptide binding partner for the peptide tag, wherein the two fragments are capable of covalent recombination through isopeptide bond formation, thereby linking the molecule or component fused to the peptide tag and its polypeptide binding partner. The isopeptide bond formed by the peptide tag and its polypeptide binding partner is stable under conditions where non-covalent interactions will rapidly dissociate, for example, over a long period of time (e.g., several weeks), at high temperatures (up to at least 95 ℃), high forces, or harsh chemical treatments (e.g., pH 2-11, organic solvents, detergents, or denaturants).

Isopeptide bonds are amide bonds formed between carboxyl/carboxamide groups and amino groups, wherein at least one carboxyl or amino group is outside the protein backbone (the backbone of the protein). Such bonds are chemically irreversible under typical biological conditions, and they are resistant to most proteases. Since isopeptide bonds are covalent in nature, they result in some of the strongest protein interactions measured.

Briefly, two-part linkers, i.e. peptide tags and their polypeptide binding partners (so-called peptide tag/binding partner pairs), may be derived from proteins capable of spontaneously forming isopeptide bonds (isopeptide proteins), wherein the domains of the proteins are expressed separately to yield a peptide "tag" comprising one of the residues involved in isopeptide bonds (e.g. aspartic acid or asparagine, or lysine) and a peptide or polypeptide binding partner (or "catcher") comprising the other residue involved in isopeptide bonds (e.g. lysine, or aspartic acid or asparagine) and at least one other residue required for the formation of isopeptide bonds (e.g. glutamic acid). Mixing the peptide tag and the binding partner results in spontaneous formation of an isopeptide bond between the tag and the binding partner. Thus, by incorporating the peptide tag and the binding partner separately into different molecules or components, e.g. proteins, it is possible to covalently link the molecules or components by isopeptide bonds formed between the peptide tag and the binding partner, i.e. to form a linker between the molecules or components incorporating the peptide tag and the binding partner.

The spontaneous formation of isopeptide bonds can be isolated and does not require the addition of any other entities. For some peptide tags and tag partner pairs, the presence of an auxiliary entity such as a ligase may be required to generate the isopeptide bond.

The peptide tag/binding partner pair (two-part linker), designated SpyTag/SpyCatcher, is derived from the CnaB2 domain of the Streptococcus pyogenes Fbab protein (Zakeri et al, 2012, Proc Natl Acad Sci U S A109, E690-697) and is used in a variety of applications including vaccine development (Brune et al, 2016, Scientific reports 6,19234; Thrane et al, 2016, Journal of Nanobiotechnology 14, 30).

Suitably, the first and second peptide tags form a peptide tag/binding pair, referred to as SpyTag/SpyCatcher. Suitably, the spycacher component is DeltaN1(Δ N1) SpyCatcher (as described in Li, l., Fierer, j.o., Rapoport, T.A. & Howarth, m.structural analysis and optimization of the covalent association between the spycatter and a peptide tag.j.mol.biol.426,309-317 (2014)), DeltaN1(Δ N1) SpyCatcher having a truncation of 23 amino acids at the N-terminus (SEQ ID No.38) as compared to "SpyCatcher".

In other embodiments, the first and second peptide tags form a peptide tag/binding pair that is a mutant form of SpyTag/SpyCather that exhibits an increased rate of isopeptide bond formation reaction, such as, for example, those described in co-pending application GB 1706430.4. In some embodiments, these mutant forms may be useful in situations where attachment and/or slow reaction or steric hindrance of large proteins (e.g., >50kDa or >100kDa, such as the >160kDa HCMV pentamer proteins described herein) may be an issue.

In other embodiments, the isopeptide protein may comprise a snoeptag/snooppactcher, described, for example, in WO 2016/193746.

In some embodiments, one or both of the isopeptide proteins may have an N-terminal truncation or a C-terminal truncation, while still maintaining the reactivity of the isopeptide bonds.

Exemplary first and second peptide tag pairs (peptide tag/binding partner pair; reactive pair) are described in the following table:

described, for example, in WO2011/098772, WO2016/193746, GB1706430.4, GB 1705750.6 or Li, l., et al, j.mol.biol.426,309-317 (2014).

Variants, derivatives and modifications of binding pairs may be made by any suitable means. Variants, derivatives and functional manipulation modifications may involve additions, substitutions, alterations or deletions of amino acids that retain the same function in terms of their ability to form isopeptide bonds with the relevant binding partner.

For some binding pairs, mediation by a third entity, such as an enzyme, is required. For example, snooppligase may be used to mediate bond formation between snooppgajr and DogTag. Thus, pairing may require the assistance of enzymes such as ligases.

HBsAg

"HBsAg" means the surface antigen from hepatitis B virus (HBsAg) or a portion thereof. In one embodiment, HBsAg may refer to the N-terminus of HBsAg, such as the HBsAg sequence set forth in SEQ ID NO:41, comprising 226 amino acids of the S protein of hepatitis B virus (adw serotype). Suitably, the HBsAg comprises the four amino acid sequence Pro Val Thr Asn representing the four carboxy terminal residues of the hepatitis B virus (adw serotype) preS2 protein, as described in Valenzuela et al, (1979) 'Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen' Nature 280: 815-819. VLPs formed from HBsAg have been approved for clinical use against hepatitis B (Kushnir et al Vaccine 2012; Vol 31(1):58-83), including Recombivax HB (https:// vaccins. procon. org/sourcefiles/Recombivax _ package _ insert. pdf) and Energix B (https:// au. gsk. com/media/217195/angerix-B _ pi _006_ approximate. pdf). HBsAg has also been used as the basis for pre-erythrocytic malaria Vaccine RTS, S, which has completed phase III clinical trials and is the most advanced malaria Vaccine to date (http:// www.malariavaccine.org/sites/www.malariavaccine.org/files/content/pag e/files/RTSS% 20FAQs _ FINAL. pdf; Kaslow and Biernaux, Vaccine 2015, Vol.33(52): 7425-.

Details of joints

The distance between proteins (e.g., VLPs and decoration antigens) can have an effect on the availability of epitopes in the protein, the stability of the protein, and can also have an effect on conjugation efficiency due to the accessibility of any isopeptide bond partner (e.g., SpyTag/SpyCatcher). Thus, a linker with suitable characteristics may be selected to optimize usability, stability and/or accessibility. The joint can be broadly subdivided into flexible and rigid sub-types.

Flexible joint

When the attached domain needs to be moved, a flexible linker can be used. They usually consist of small non-polar (e.g., Gly) or polar (e.g., Ser, Thr) amino acids, with small size providing flexibility (Chen et al, 2013Adv Drug Deliv Rev. Oct 15; 65(10): 1357-. Addition of Ser or Thr can help maintain stability in solution, and adjusting the length can affect correct folding of the protein (Chen et al, 2013). Any suitable flexible joint having properties and length suitable for the entity of interest may be used. Suitably, the flexible linker may comprise between 2 and 70 combinations of amino acids of this type.

Example (c):

rigid joint

In some cases, rigid linkers may be preferred because they may help provide protein separation. The rigid joint has a secondary structure. One of the most common rigid joints is (EAAAK)_n(where n is the number of repeats) which adopt an alpha-helical structure (Arai et al, (2001) Protein Eng. Aug; 14(8): 529-32). Other rigid linkers may include proline rich sequences such as (XP)_nWherein X is any amino acid, but preferably Ala (A), Lys (K) or Glu (E), wherein proline provides a conformational constraint (Chen et al, 2013).

Other suitable linkers are exemplified by Klein et al (2014) Protein Eng Des sel.oct; 27(10) 325 and 330. Any suitable rigid joint having properties and length suitable for the entity of interest may be used. Suitably, the rigid linker may comprise between 2 and 70 combinations of amino acids of this type.

Example (c):

sequence name	Sequence of	SEQ ID No
			Rigid joint 1	EAAAK	SEQ ID NO：9
Rigid joint 2- (EAAAK)3	EAAAKEAAAKEAAAK	SEQ ID NO：10
			Rigid joint 3- (AP)7	APAPAPAPAPAPAP	SEQ ID NO：11

Host cells and expression vectors

One skilled in the art will know suitable host cells for expressing nucleic acids to produce proteins and compositions according to the present invention.

In one embodiment, the host cell will be suitable for transient expression. In another embodiment, the host cells will be those capable of forming a stable cell line. Suitably, coding sequences encoding antigenic components such as HCMV pentamer and RSV-F proteins (including those comprising peptide tags forming isopeptide bonds) will be integrated into a host cell. In one embodiment, each nucleic acid sequence encoding a subunit of a multimer, such as a pentamer, will be contained in a different plasmid/vector, such that transfection of a host cell with, for example, all 5 plasmids/vectors will result in the production of a pentamer by the host cell when cultured under suitable conditions. In other embodiments, the plasmid/vector may comprise a combination of one or more coding sequences such that at least 1, 2,3, 4, or 5 plasmids may be introduced. Alternatively, the entire fusion peptide coding sequence may be provided in one vector, such that the entire protein component and the first peptide tag are encoded on the same vector.

In one embodiment, these vectors are used to stably integrate the coding sequence into the genome of the host cell. Suitable host cells for stable expression include mammalian cells, such as HEK cells (human embryonic kidney 293 cells) or rodent cells, including CHO (chinese hamster ovary) cells. Suitable mammalian cells and vectors for expressing the protein component of the composition according to the invention will be known to the person skilled in the art and described, for example, in WO2016/067239, pages 15-16 and Hofmann et al, (2015) Biotech and bioenng, 112(12): 2505-. Exemplary stabilizing construct sequences for expressing components according to the invention can be found in example 3 below.

Affinity purification

In some embodiments, those expression constructs used to express components of compositions according to the invention may comprise "tag" sequences or sequences that facilitate purification, such as affinity purification. Any suitable tag, such as an affinity tag, may be included to separate the protein component and the first peptide tag from the system that produced the protein component and the first peptide tag. Those skilled in the art of recombinant protein production know of systems such as His-tags and Strep-tags that may be included for purification purposes. Such tags greatly facilitate protein purification and have little adverse effect on biological or biochemical activity, and are therefore desirable. Suitable tag sequences include a C-tag, a histidine tag (His-tag), a streptavidin tag (Strep-tag), Maltose Binding Protein (MBP), glutathione-S-transferase (GST), and a FLAG tag.

Both the protein component and/or the moiety may comprise an affinity purification tag. For ease of use, these are usually genetically fused at the C-terminus or N-terminus of the protein.

Thus, in some embodiments, for example, the gH, gL, pUL128, pUL130, pUL131A (or fragments thereof) subunits of HCMV, RSV pre-fusion F proteins, or HBsAg peptides/proteins may comprise additional amino acid residues at the N-terminus or C-terminus, which facilitates purification. Such additional amino acid residues may include a tag, such as, for example, a His-tag or a C-tag. In some embodiments, the C-tag can provide cleaner purification. Other suitable tag sequences include Maltose Binding Protein (MBP), Strep-tag, glutathione-S-transferase (GST), and FLAG tag. In some embodiments, the tag may be attached to the amino acid sequence in such a way that the tag can be cleaved after purification, for example using a cleavable linker, for example. In other embodiments, non-affinity purification methods may be used.

In other embodiments, the RSV pre-fusion F protein can comprise additional amino acid residues at the C-terminus or N-terminus, which aid in purification. As exemplified herein, the RSV pre-fusion F protein has a C-tag for affinity purification.

Conjugation of first and second peptide tag pairs

The conjugation of the first and second peptide tag/binding partner/reactive pair may be performed overnight at 4 ℃. Alternatively, the conjugation reaction can be carried out at room temperature for 3-4 hours, since the coupling rate is expected to increase at room temperature. The optimal ratio of first and second binding partners for a particular coupling reaction depends on the size of each binding partner. For example, for smaller antigens (-20 kDa), a molar ratio of VLP monomers to antigen of 1:1.5 may be sufficient, while for larger antigens (r) ((r))>100kDa), a mass ratio of 1:1 may be sufficient in combination with the same VLP monomer. However, both ratios result in an excess of antigen (smaller binding partner). Any excess antigen can be removed by, for example, Size Exclusion Chromatography (SEC) or by dialysis. Dialysis may be more suitable for smaller antigens because it is less effective than SEC. Alternatively, the VLP/particle to antigen ratio can be optimized such that all antigens are conjugated and therefore downstream purification is not required. A suitable final protein concentration of about 1mg/ml is optimal for the conjugation reaction, since lower concentrations decrease the reaction rate. A wide variety of buffers near neutral pH are compatible with coupling/conjugation. The standard choice of conjugation buffer was TBS (20mM Tris and 150mM NaCl, pH 7.4). In some cases, addition may be used, as described by Brune et al Sci Rep. (2016)Add 10 Xstock of citrate buffer (40mM Na)₂HPO₄200mM sodium citrate, pH 6.2).

Pharmaceutical composition and use

The compositions of the invention may be incorporated into vaccines or immunogenic compositions. Suitably, the vaccine or immunogenic composition will comprise an immunogenic dose of the particles of the invention.

The pharmaceutical composition may comprise particles or immunogenic compositions according to the invention provided together with a pharmaceutically acceptable carrier. Suitable carriers are well known to those of ordinary skill in the art. In one embodiment, the pharmaceutical composition comprises a buffer, excipient or carrier. Suitably, the pharmaceutical composition may comprise suitable excipients and formulations to maintain the stability of the composition. Suitably, the formulation may comprise an adjuvant. In one embodiment, the formulation may comprise a formulation similar to that ofAddavax of^TMOr similar squalene-based oil-in-water nanoemulsions. Other suitable adjuvants include liposome-based adjuvants such AS matric m (matrix m) and AS 01. Other suitable adjuvants include aluminum-based formulations, such asIn one embodiment, the formulation may comprise EDTA, for example, at a concentration of 5 mM. Suitable excipients or formulations may depend on the nature of the particles or immunogenic composition; for example, the choice of expression system may affect the stability, glycosylation or folding of the protein in the composition, which may in turn affect the optimal formulation of the composition. Methods of determining suitable excipients, formulations or adjuvants will be known to those skilled in the art.

Various other aspects and embodiments of the invention will be apparent to those skilled in the art in view of this disclosure.

All documents mentioned in this specification are herein incorporated in their entirety by reference.

As used herein, "and/or" should be considered a specific disclosure of each of the two specified features or components, with or without the other. For example, "a and/or B" shall be considered a specific disclosure of each of (i) a, (ii) B, and (iii) a and B, as if each were individually listed herein.

Unless the context indicates otherwise, the description and definition of features listed above is not limited to any particular aspect or embodiment of the invention, and applies equally to all aspects and embodiments described.

It will also be appreciated by persons skilled in the art that the present invention has been described by way of example with reference to several embodiments. It is not limited to the embodiments disclosed and alternative embodiments may be constructed without departing from the scope of the invention as defined in the following claims.

"recombinant" as used herein to describe a polynucleotide means a polynucleotide of genomic, cDNA, semisynthetic, or synthetic origin, which, due to its origin or manipulation: (1) not related to all or part of the polynucleotide with which it is associated in nature; and/or (2) to a polynucleotide other than that to which it is linked in nature. The term "recombinant" as applied to protein or polypeptide aspects means a polypeptide produced by expression of a recombinant polynucleotide.

Unless otherwise specified, the processes comprising the steps may be performed in any suitable order. Accordingly, these steps may be performed in any suitable order.

Sequence identity between polypeptide sequences is preferably determined by a pairwise alignment algorithm using the Needleman-Wunsch global alignment algorithm (Needleman and Wunsch1970), using default parameters (e.g., using the EBLOSUM62 scoring matrix, a gap opening penalty of 10.0, and a gap extension penalty of 0.5). This algorithm is conveniently implemented in the needle tool in the EMBOSS software package (Rice, Longden and Bleasby 2000). Sequence identity should be calculated over the entire length of the polypeptide sequence of the invention.

Any homologue of a component mentioned herein is typically a functional homologue and is typically at least 40% homologous to the relevant region of the protein. Homology can be measured by known methods. For example, the UWGCG Package provides the BESTFIT program (e.g., used in its default settings) that can be used to calculate homology (Devereux et al (1984) Nucleic Acids Research 12, 387-395). The PILEUP algorithm and the BLAST algorithm can be used to calculate homology or aligned sequences (line up sequences) (usually based on their default settings), for example as described in Altschul S.F, (1993) J Mol Evol 36: 290-300; altschul, S, F et al (1990) J Mol Biol 215: 403-10. Software for performing BLAST analysis is publicly available through the National Center for Biotechnology Information (http:// www.ncbi.nlm.nih.gov /).

The BLAST algorithm performs a statistical analysis of the similarity between two sequences; see, e.g., Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 90: 5873. sup. 5787. One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P (N)) that provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a sequence is considered similar to another sequence if the smallest sum probability in a comparison of the first sequence to the second sequence is less than about 1, preferably less than about 0.1, more preferably less than about 0.01, and most preferably less than about 0.001.

A variant polypeptide comprises (or consists of) a sequence that is at least 40% identical to a native protein. In preferred embodiments, the variant sequence may be at least 55%, 65%, 70%, 75%, 80%, 85%, 90% and more preferably at least 95%, 97% or 99% homologous to a particular region of the native protein over at least 20, preferably at least 30, such as at least 40, 60, 100, 200, 300, 400 or more contiguous amino acids, or even over the entire sequence of the variant. Alternatively, the variant sequence may be at least 55%, 65%, 70%, 75%, 80%, 85%, 90% and more preferably at least 95%, 97% or 99% homologous to the full-length native protein. Typically, the variant sequence differs from the relevant region of the native protein by at least 2, 5, 10, 20, 40, 50 or 60 mutations or by less than 2, 5, 10, 20, 40, 50 or 60 mutations (each mutation may be a substitution, insertion or deletion). A variant sequence of the invention may have a certain percentage of identity with a particular region of a full-length native protein that is the same as any of the particular percentage of homology values across any length of the sequence mentioned above (i.e., it may have at least 40%, 55%, 80% or 90% and more preferably at least 95%, 97% or 99% identity).

Variants of the protein also include truncations. Any truncation can be used, provided that the variant is still functional. Truncation is typically performed to remove sequences that are not essential for activity/function, particularly isopeptide bond formation, and/or do not affect the conformation of the folded protein, particularly the folding of any immunogenic sites. Truncations may also be chosen to improve the ease of component generation. Suitable truncations can generally be identified by systematically truncating sequences of varying length from the N-terminus or the C-terminus.

Variants of a native protein also include mutants having one or more, e.g., 2,3, 4, 5 to 10, 10 to 20, 20 to 40, or more, amino acid insertions, substitutions, or deletions relative to a particular region of the native protein. Deletions and insertions are preferably made outside the antigenic region. Insertions are usually made at the N-terminus or C-terminus of sequences derived from the native protein, for example for the purpose of recombinant expression. Substitutions are also typically made in regions that are not essential for activity/function and/or do not affect the conformation of the folded protein. Such substitutions may be made to improve the solubility or other characteristics of the protein. To increase the stability of the protein, substitutions may be made.

The substitution preferably introduces one or more conservative changes that replace the amino acid with other amino acids that are similar in chemical structure, similar in chemical nature, or similar in side chain volume. The amino acids introduced may have similar polarity, hydrophilicity, hydrophobicity, basicity, acidity, neutrality or charge as the amino acids they replace. Alternatively, a conservative change may introduce another aromatic or aliphatic amino acid in place of a pre-existing aromatic or aliphatic amino acid. Conservative amino acid changes are well known in the art.

A derivative is an entity that is produced or prepared from a parent entity by replacing some portion of the parent entity.

Examples

Example 1

Production of an exemplary multimer-VLP composition (HCMV pentamer-HBsAg VLP)

Expifeacmine was used^TM293 transfection reagent (ThermoFisher Scientific) and 5 independent plasmids encoding the following sequences transiently expressed HCMV pentamer in Expi293F cells. The HCMV pentamer described below is about 162kDa, aglycosylated (including tag and linker, but not including signal peptide).

Nucleotide sequence

The expressed HCMV pentamer sequence represents the native sequence from the Merlin strain (GenBank: AY 446894.2; low passage (i.e.attenuated) HCMV strain) (containing introns) except for the two introduced mutations (one in gH and one in UL 128), as described in the relevant paragraphs below.

gH-SpyTag-His nucleotide sequence (SEQ ID NO.12)

In this sequence (SEQ ID NO:12), a silent mutation C was introduced at position 1146>A is used forSynthesis, since the natural sequence CACCTGC around this nucleotide is marked as potentially problematic. The construct comprises: signal peptide (nt 1-69), extracellular domain (nt 70-2151), transmembrane domain (truncated) (nt 2152) -2157), (signal peptide, extracellular domain and transmembrane domain (truncated) together are represented by SEQ ID NO:13), linker (nt 2158-2175; SEQ ID NO:14), SpyTag (nt 2176-2214; SEQ ID NO:15), 6 xHis-tag (nt 2215-2232), stop codon (nt 2233-2235). Nucleotides 1 to 2157(SEQ ID NO:13) represent the gH coding sequence.

gL nucleotide sequence (SEQ ID NO.16)

In this sequence: a signal peptide (nt 1-90), an extracellular domain (nt 91-834), and a stop codon (nt 835-837).

UL130-C tag nucleotide sequence (SEQ ID NO.17)

In this sequence: signal peptide (nt 1-75), extracellular domain (nt 76-642), linker (nt 643-687), C-tag (nt 688-699), stop codon (nt 700-702).

UL128 nucleotide sequence (SEQ ID NO.20) (containing 2 introns present in the native sequence)

In this sequence: signal peptide (nt 1-81), intron: nt 165-287, nt 423-542, the extracellular domain exons (nt 82-164, nt 288-422, nt 543-756), and the stop codon (nt 757-759).

A T > C mutation was introduced at nucleotide 634. It is mentioned in the GenBank document that the T634 nucleotide causes premature termination of UL128 in the Merlin strain, and therefore we used comments from different strains (GenBank: GQ396662.1, strain HAN38) to tell which base to replace in order to restore expression to the full-length protein.

UL131A nucleotide sequence (SEQ ID NO.21) (containing introns present in the native sequence)

In this sequence: signal peptide (nt 1-54), intron (nt 237-.

SpyCatcher-HBsAg nucleotide sequence (SEQ ID NO.22)

In this sequence: SpyCatcherDeltaN1(nt 1-276), flexible linker (nt 277-.

Amino acid sequence

Expression of the above nucleotide sequence is expected to yield the following amino acid sequence.

gH-SpyTag-His amino acid sequence (SEQ ID NO.27)

The expected mass is 81.852kDa (no signal peptide), 84.364kDa (including signal peptide).

In this sequence: signal peptide (aa 1-23), extracellular domain (aa 24-717), transmembrane domain (truncated) (aa 718-), (signal peptide, extracellular domain and transmembrane domain (truncated) together are represented by SEQ ID NO:28), linker (aa 720-), (aa 725; SEQ ID NO:29), SpyTag (aa 726-), (738; SEQ ID NO:30), 6 × His-tag (aa 739-), (744). Amino acid residues 1-719 represent the natural Merlin strain gH amino acid sequence with a truncated TM domain (SEQ ID NO: 28).

gL amino acid sequence (SEQ ID NO:31)

The expected mass is 27.522kDa (no signal peptide), 30.815kDa (including signal peptide).

In this sequence: signal peptide (aa 1-30), extracellular domain (aa 31-278). Amino acid residues 1-278 represent the gL amino acid sequence of the native Merlin strain.

UL130-C tag amino acid sequence (SEQ ID NO:32)

The expected mass is 23.167kDa (no signal peptide), 26.081kDa (including signal peptide).

In this sequence: signal peptide (aa 1-25), extracellular domain (aa 26-214), (signal peptide and extracellular domain together represented by SEQ ID NO: 33), linker (aa 215-.

Amino acid residues 1-214 represent the natural Merlin strain UL130 amino acid sequence.

UL128 amino acid sequence (SEQ ID NO:35)

The expected mass is 16.659kDa (no signal peptide), 19.717kDa (including signal peptide).

In this sequence: signal peptide (aa 1-27), extracellular domain (aa 28-171). Amino acid residues 1-171 represent the natural Merlin strain UL128 amino acid sequence.

UL131A amino acid sequence (SEQ ID NO:36)

The expected mass is 12.985kDa (no signal peptide), 14.989kDa (including signal peptide).

In this sequence: signal peptide (aa 1-18), extracellular domain (aa 19-129). Amino acid residues 1-129 represent the amino acid sequence of native Merlin strain UL 131A.

SpyCatcher-HBsAg amino acid sequence (SEQ ID NO:37)

The expected mass is 36.824kDa, including the tag and the linker.

In this sequence: SpyCatcherDeltaN1(aa 1-92; SEQ ID NO:38), flexible linkers (aa 93-101; SEQ ID NO:39), PVTN linkers (aa 102-105; SEQ ID NO:40), HBsAg (aa 106-331; SEQ ID NO:41), C-tag (aa 332-335).

Purification of pentamers

The pentamer-SpyTag is expressed in EXPI293F cells and secreted into the supernatant (due to the deletion of the TM domain (part of it) of the gH subunit). Initial attempts to purify HCMV pentamers using affinity purification relied on expression of a C-tagged gH subunit, but this resulted in the isolation of gH/gL heterohomodimers as well as pentamers. In an alternative strategy, a C-tag was added to the UL130 subunit (SEQ ID NO:17 (nucleotides) and SEQ ID NO:32 (amino acids)), which allowed the pentamer to be purified from the supernatant using C-tag affinity purification (ThermoFisher) and size exclusion chromatography. When analyzed by SDS-PAGE, the pentamer appeared as expected under non-reducing conditions and reducing conditions (FIG. 1A) and reacted with anti-HCMV pentamer antibody (Native Antigen Company (AbCMV2450)) (FIG. 1B), with only a small amount of contaminant observed at-14 kDa.

Purification of HBsAg VLP monomers

SpyCatcher-HBsAg was expressed in Pichia pastoris (Pichia pastoris) and purified from the cell homogenate. On an SDS-PAGE gel under reducing conditions, the major protein band corresponds to the expected size of the monomer (about 37kDa), the larger band indicating the presence of oligomeric material, indicating good cross-linking of the particles (fig. 2A, lane 'R'). Under non-reducing conditions (lane 'NR'), material remained predominantly on top of the gel with some tailing (smearing), indicating that the VLP particles formed well and were therefore too large to migrate completely into the gel (fig. 2A). Both non-reduced and reduced SpyCatcher-HBsAg reacted strongly with mouse anti-HBsAg monoclonal antibody (obtained from Bio-Rad (MCA4658)) (fig. 2B), indicating that the presence of SpyCatcher did not negatively affect the reactive epitope. Both HCMV pentamer-SpyTag and SpyCatcher-HBsAg eluted as a single peak on a s200 inch 3.2/300 column as assessed by HPLC size exclusion analysis (FIGS. 3A-3B). The HCMV pentamer-SpyTag eluted at about 400kDa (FIG. 3A), which is greater than expected. However, this can be explained by the fact that the pentamer is not spherical in structure, which is known to alter the retention time of the protein during size exclusion chromatography. SpyCatcher-HBsAg eluted in the column's outer water volume, indicating that the particles were formed correctly and there was no detectable monomer in the solution (fig. 3B).

antigen-VLP conjugation

HCMV pentamer-SpyTag and Spycatcher-HBsAg were conjugated overnight at 4 ℃ to obtain HBsAg VLP coated with HCMV pentamer. A buffer containing Tris buffered saline (TBS: 20mM Tris and 150mM NaCl, pH 7.4) supplemented with 5mM EDTA was used for conjugation. Conjugation was monitored using SDS-PAGE and western blot analysis as well as HPLC. When the conjugation reaction was compared to pentamer-SpyTag or SpyCatcher-HBsAg alone, a new band at-130 kDa was present under reducing conditions (fig. 4A, lane 2), which reacted with both monoclonal anti-HBsAg (fig. 4B) and polyclonal anti-HCMV pentamer (fig. 4C) antibodies, indicating that it contained at least conjugated HBsAg-gH. When analyzed by HPLC size exclusion chromatography, 97% of the eluate in the main peak corresponds to the expected size of the conjugated HCMV pentamer-HBsAg monomer (fig. 5).

Example 2

In vivo testing of HCMV-SpyTag- -SpyCatcher-HBsAg VLP (adjuvanted)

Conjugated HCMV pentamer-HBsAg VLPs and unconjugated HCMV pentamer-SpyTag are used in an immunization protocol using BALB/c mice to (i) confirm the immunogenicity of the HCMV pentamer-SpyTag produced, and (ii) compare the immunogenicity of the unconjugated HCMV pentamer-SpyTag to the conjugated HCMV pentamer-HBsAg VLPs.

The following prime-boost regimen with 3 week intervals was used:

day 0: immunization (priming); day 20: taking blood from the tail; day 21: immunization (boost 1); day 41: taking blood from the tail; day 42: immunization (boost 2); day 63: the heart was bled.

The groups immunized were as follows. For each group n 10:

1)AddaVax^TM(Invivogen) 1. mu.g of HCMV pentamer-SpyTag

2)AddaVax^TM1 μ g of HCMV pentamer-SpyTag- -SpyCatcher-HBsAg VLP (1 μ g pentamer equivalent)

3)AddaVax^TMMiddle Spycatcher-HBsAg VLP (normalized against the amount of Spycatcher-HBsAg in group 2)

4)AddaVax^TM0.1. mu.g of HCMV pentamer-SpyTag

5)AddaVax^TM0.1. mu.g of HCMV pentamer-SpyTag- -SpyCatcher-HBsAg VLP (0.1. mu.g pentamer equivalent)

6) TBS (20mM Tris and 150mM NaCl, pH 7.4)

AddaVax^TMIs a formula similar toHas obtained influenza vaccine adjuvant permission in europe. Squalene oil-in-water emulsions are known to elicit cellular (Th1) and humoral (Th2) immune responses. Other suitable adjuvants will be known to those skilled in the art.

Immunogenicity was assessed using ELISA. The titers of antisera produced in each group were determined using a standardized ELISA against HCMV pentamer. Plates were coated with 50. mu.L/well of 5. mu.g/ml pentamer (no SpyTag) overnight; washing; sealing with milk for 1 hour; washing; mouse serum (diluted appropriately in PBS) was applied for 1 hour; washing; goat anti-mouse-alkaline phosphatase antibody (1:10,000) was applied for 1 hour; washing; and (4) developing color.

Different doses of unconjugated (groups 1 and 4) and conjugated HCMV pentamer-HBsAg (groups 2 and 5) were included to allow comparison of immunogenicity between conjugated HCMV pentamer-HBsAg VLP vaccines and unconjugated HCMV pentamer-SpyTag, which allows extrapolation to other HCMV pentamer vaccines (e.g. soluble pentamers). Groups 3 and 6 represent negative controls.

At each time point, the OD value of the sample was read at the appropriate dilution and ELISA units were determined using a standard curve run on each plate. Data showing the results of the priming of groups 1, 2, 4 and 5 are shown in figure 6. Mice immunized with HCMV pentamer-HBsAg at doses of 1 μ g and 0.1 μ g showed significantly stronger serum IgG antibody responses compared to mice immunized with unconjugated HCMV pentamer at doses of 1 μ g or 0.1 μ g. The ELISA units of groups 3 and 6 provide the baseline for this assay, also shown in fig. 6.

The functional activity of the produced antibodies was investigated using a micro-neutralization assay based on Wang et al (Vaccine 33(2015) 7254-7261; DOI:10.1016/j. vaccine.2015.10.110). The neutralization titers of group 1, group 2, group 4 and group 5 are shown in figure 7. Sera from mice immunized with the pentamer-HBsAg VLP were significantly more neutralizing than sera from mice immunized with the pentamer-SpyTag protein alone.

Example 3

Stable construct sequences

Two stable constructs (adapted from Hofmann et al, (2015) Biotech and Bioeng,112(12):2505-2515) were optimized for CHO expression of the components of the HCMV pentamer-SpyTag. Introns were removed from the HCMV pentamer sequence, but the signal sequence was retained.

HCMV gH-SpyTag/gL stable expression construct

Stable vector construct HCMV-gH- (GSG)₂SpyTag-His-IRES-gL was designed to contain a gH-SpyTag-His component (SEQ ID NO:42) and a gL component (SEQ ID NO:43) upstream and downstream of EV71 IRES, respectively. The coding sequences used in this construct are described below.

Nucleotide sequence

gH- (GSG)2-SpyTag-His (intron-free) (SEQ ID NO:42) insertion into EV71 IRES upstream

In this sequence: signal peptide (nt 1-69), extracellular domain (nt 70-2151), truncated transmembrane domain (nt 2152-2157), (GSG)₂Linker (nt 2158-.

gL (without intron) (SEQ ID NO:43) inserted downstream of EV71 IRES

In this sequence: a signal peptide (nt 1-90), an extracellular domain (nt 91-834), and a stop codon (nt 835-837).

HCMV UL128/UL130/UL131A stable expression construct

The stable construct HCMV-UL128-IRES-UL130- (G4S)3-C tag-IRES-UL 131A was designed to comprise a UL128 component (SEQ ID NO:44), a UL130 component (SEQ ID NO:45) and a UL131A component (SEQ ID NO: 46). The UL130 component was inserted after the first EV71 IRES of the plasmid, and the UL131A component was inserted after the second EV71 IRES. The coding sequences used in this construct are described below.

Nucleotide sequence

Ul128 (without intron) (SEQ ID NO:44)

In this sequence: signal peptide (nt 1-81), extracellular domain (nt 82-513), stop codon (nt 514-516).

UL130- (G4S)3-C tag (without intron) (SEQ ID NO:45)

In this sequence: signal peptide (nt 1-75), extracellular domain (nt 76-642), (G4S)₃Linker (nt 643-687), C tag (nt 688-699), stop codon (nt 700-702).

UL131A (without intron) (SEQ ID NO:46)

In this sequence: signal peptide (nt 1-54), extracellular domain (nt 55-387), stop codon (nt 388- & 390).

Example 4

In vivo detection of HCMV-SpyTag-SpyCatcher-HBsAg VLP (without adjuvant)

Conjugated HCMV pentamer-HBsAg VLPs and unconjugated HCMV pentamer-SpyTag were used in immunization protocols using BALB/c mice to further study the immunogenicity of conjugated pentamer-HBsAg VLPs versus unconjugated pentamer-SpyTag protein.

The following prime-boost regimen with 3 week intervals was used:

day 0: immunization (priming); day 20: taking blood from the tail; day 21: immunization (boost 1); day 41: taking blood from the tail; day 42: immunization (boost 2); day 63: the heart was bled.

The groups immunized were as follows. For each group n 10:

1)1 μ g HCMV pentamer-SpyTag, without adjuvant

2)1 μ g HCMV pentamer-SpyTag-Spycatcher-HBsAg VLP (1 μ g pentamer equivalent), without adjuvant

3)0.1 μ g HCMV pentamer-SpyTag- -Spycatcher-HBsAg VLP (0.1 μ g pentamer equivalent), without adjuvant

Immunogenicity was assessed using ELISA. The titers of antisera produced in each group were determined using a standardized ELISA against HCMV pentamer. Plates were coated with 50. mu.L/well of 5. mu.g/ml pentamer (no SpyTag) overnight; washing; sealing with milk for 1 hour; washing; mouse serum (diluted appropriately in PBS) was applied for 1 hour; washing; goat anti-mouse-alkaline phosphatase antibody (1:10,000) was applied for 1 hour; washing; and (4) developing color.

At each time point, the OD value of the sample was read at the appropriate dilution and ELISA units were determined using a standard curve run on each plate. The data after priming and after boosting are shown in fig. 8. Mice immunized with HCMV pentamer-HBsAg at both 1 μ g and 0.1 μ g doses showed significantly stronger serum IgG antibody responses than mice immunized with 1 μ g HCMV pentamer alone as soluble protein.

The functional activity of the produced antibodies was investigated using a micro-neutralization assay based on Wang et al (2015). The neutralization titers after priming and after boosting are shown in figure 9. Sera from mice immunized with the pentamer-HBsAg VLP without adjuvant had a significantly more neutralizing effect than sera from mice immunized with the pentamer-SpyTag protein alone without adjuvant.

Example 5

Expression and purification of RSV-F-SpyTag

Fusion of sequences from the antigen RSV-F Sc9-10DS-Cav 1A 149C Y458C to SpyTag to generate RSV-F-SpyTag, and use of ExpicHO^TMExpression system kit and Expifeacylamine^TMTransfection reagent (ThermoFisher Scientific) by transient transfection of ExpicCHO with plasmid pcDNA3.4 containing the nucleotide sequence SEQ ID NO:47^TMCells to express.

RSV-F Sc9-10DS-Cav 1A 149C Y458C (National Institutes of Health)) is a variant of respiratory syncytial virus fusion protein (pre-fused RSV-F) as described in Joyce et al (2016) (Iterative structural-based improvement of respiratory syndrome virus fusion protein vaccine. Nat Structure Mol biol. 2016Sep; 23(9): 811-820). This variant is a pre-fused stabilized form of a fusion (F) glycoprotein with genetically linked F subunits, deleted fusion peptide, T4 fibritin trimerisation motif (folding domain) and interpolymeric movement stabilized by an additional interpolymeric disulphide bond (a149C Y458C).

Nucleotide sequence

RSV-F-SpyTag-C tag nucleotide sequence (SEQ ID NO:47)

By deletion of the thrombin site, 6 × His-tag andII original sequence of Sc9-10DS-Cav 1A 149C Y458C was modified. These deleted domains were replaced by a linker-SpyTag-C tag sequence to generate a 1587nt cassette (SEQ ID NO:47) comprising Sc9-10DS-Cav 1A 149C Y458C (nt 1-1515 comprising the signal peptide (nt 1-75) and the T4 fibritin foldon domain (nt 1435-1515)), (GSG)₂Linker (nt 1516-1533; SEQ ID NO:14), SpyTag (nt 1534-1572; SEQ ID NO:15), C-tag (nt 1573-1584) and stop codon (nt 1585-1587). The Sc9-10DS-Cav 1A 149C Y458C nucleotide sequence, which does not contain a linker, SpyTag, a C-tag and a stop codon, is contained in SEQ ID NO 48. The Sc9-10DS-Cav 1A 149C Y458C nucleotide sequence that does not contain a signal peptide, a linker, a SpyTag or a C-tag is contained in SEQ ID NO. 49.

Amino acid sequence

Expression of the nucleotide sequence SEQ ID NO:47 is expected to yield a RSV-F-SpyTag-C tag amino acid sequence (SEQ ID NO:50) with the following domains: sc9-10DS-Cav 1A 149C Y458C ((aa 1-505 comprising signal peptide (aa 1-25) and foldon domain (aa 479-) -505)), linker (aa 506- & 511; SEQ ID NO:29), SpyTag (aa 512- & 524; SEQ ID NO:30), C-tag (aa 525- & 528).; the predicted mass of the protein with signal peptide is 57.9kDa, and the predicted mass of the protein without signal peptide is 55.3 kDa. the Sc9-10DS-Cav 1A 149C Y458C amino acid sequence not comprising linker, SpyTag or C-tag is comprised in SEQ ID NO: 51. the Sc 5-10 DS-Cav 1A 149C Y458C amino acid sequence not comprising signal peptide, linker, SpyTag or C-tag is comprised in SEQ ID NO: 52.

Purification of RSV-F-SpyTag

RSF-F-SpyTag antigen was secreted from the cells and purified from the supernatant using C-tag affinity purification and size exclusion chromatography. RSV-F-SpyTag appeared as expected under non-reducing conditions and reducing conditions when analyzed by SDS-PAGE (FIG. 10A) and reacted with an anti-RSV-F [2F7] monoclonal antibody (ab 43812; Abcam) (FIG. 10B).

Purification of HBsAg VLP monomers

SpyCatcher-HBsAg (VLP monomers) was prepared and purified as described in example 1 above, see also fig. 2.

Conjugation of RSV-F-SpyTag to Spycatcher-HBsAg

Conjugation of RSV-F-SpyTag to Spycatcher-HBsAg overnight at 4 ℃ gave RSV-F trimer coated HBsAg VLP (RSV-F-SpyTag- -Spycatcher-HBsAg). A buffer containing Tris buffered saline (TBS: 20mM Tris and 150mM NaCl, pH 7.4) was used for conjugation. Conjugation was monitored using SDS-PAGE and western blot analysis (fig. 11). When the conjugation reaction was compared to RSV-F-SpyTag or Spycatcher-HBsAg alone, a new band at 105kDa was present under reducing conditions (FIG. 11A), which reacted with both anti-HBsAg monoclonal antibody (MCA4658, Bio-Rad) (FIG. 11B) and anti-RSV-F [2F7] monoclonal antibody (ab 43812; Abcam) (FIG. 11C), indicating that it contained conjugated RSV-F-SpyTag- -Spycatcher-HBsAg.

Example 6

Immunogenicity of conjugated RSV-F-SpyTag- -Spycatcher-HBsAg

Immunization programs using BALB/c mice were designed to confirm the immunogenicity of the resulting RSV-F antigens and to compare the immunogenicity of conjugated RSV-F-SpyTag- -SpyCatcher-HBsAg VLPs with non-conjugated RSV-F-SpyTag proteins. Groups were dosed based on the amount of RSV-F-SpyTag in the samples, and prime-boost regimens were selected with 3 week intervals, with the final time point 2 weeks after boost.

Whether the vaccine was unadjuvanted (FIG. 6) or with Addavax^TMPrepared (FIG. 6), post-priming immunization with RSV-F-SpyTag- -SpyCather-HBsAgThe mice showed a significantly stronger serum IgG antibody response compared to mice immunized with RSV-F-SpyTag protein alone.

Table of sequences

Sequence listing

<110> Sbye Biotechnology Ltd

<120> vaccine composition

<130> P9800wo1

<160> 60

<170> PatentIn version 3.5

<210> 1

<211> 6

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 2- (GSG)2

<400> 1

Gly Ser Gly Gly Ser Gly

1 5

<210> 2

<211> 9

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 3- (GSG)3

<400> 2

Gly Ser Gly Gly Ser Gly Gly Ser Gly

1 5

<210> 3

<211> 5

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 4- (G4S)1

<400> 3

Gly Gly Gly Gly Ser

1 5

<210> 4

<211> 15

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 5- (G4S)3

<400> 4

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser

1 5 10 15

<210> 5

<211> 20

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 6- (G4S)4

<400> 5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly

1 5 10 15

Gly Gly Gly Ser

20

<210> 6

<211> 12

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 7

<400> 6

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe

1 5 10

<210> 7

<211> 18

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 8

<400> 7

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser

1 5 10 15

Leu Asp

<210> 8

<211> 14

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible Joint 9

<400> 8

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Ser Thr

1 5 10

<210> 9

<211> 5

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> rigid joint 1

<400> 9

Glu Ala Ala Ala Lys

1 5

<210> 10

<211> 15

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> rigid Joint 2- (EAAAK)3

<400> 10

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys

1 5 10 15

<210> 11

<211> 14

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> rigid Joint 3- (AP)7

<400> 11

Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro

1 5 10

<210> 12

<211> 2235

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gH-SpyTag-His

<220>

<221> misc_feature

<222> (1146)..(1146)

<223> mutation C > A at position 1146

<220>

<221> misc_feature

<222> (1)..(69)

<223> Signal peptide

<220>

<221> misc_feature

<222> (70)..(2151)

<223> extracellular domain

<220>

<221> misc_feature

<222> (2152)..(2157)

<223> transmembrane domain (truncated)

<220>

<221> misc_feature

<222> (2158)..(2175)

<223> joint

<220>

<221> misc_feature

<222> (2176)..(2214)

<223> SpyTag

<220>

<221> misc_feature

<222> (2215)..(2232)

<223> 6 × His tag

<220>

<221> misc_feature

<222> (2233)..(2235)

<223> stop codon

<400> 12

atgcggccag gcctcccctc ctacctcatc atcctcgccg tctgtctctt cagccaccta 60

ctttcgtcac gatatggcgc agaagccgta tccgaaccgc tggacaaagc gtttcaccta 120

ctgctcaaca cctacgggag acccatccgc ttcctgcgtg aaaataccac ccagtgtacc 180

tacaacagca gcctccgtaa cagcacggtc gtcagggaaa acgccatcag tttcaacttt 240

ttccaaagct ataatcaata ctatgtattc catatgcctc gatgtctttt tgcgggtcct 300

ctggcggagc agtttctgaa ccaggtagat ctgaccgaaa ccctggaaag ataccaacag 360

agacttaaca cttacgcgct ggtatccaaa gacctggcca gctaccgatc tttttcgcag 420

cagctaaagg cacaagacag cctaggtgaa cagcccacca ctgtgccacc gcccattgac 480

ctgtcaatac ctcacgtttg gatgccaccg caaaccactc cacacggctg gacagaatca 540

cataccacct caggactaca ccgaccacac tttaaccaga cctgtatcct ctttgatgga 600

cacgatctac tattcagcac cgtcacacct tgtttgcacc aaggctttta cctcatcgac 660

gaactacgtt acgttaaaat aacactgacc gaggacttct tcgtagttac ggtgtccata 720

gacgacgaca cacccatgct gcttatcttc ggccatcttc cacgcgtact tttcaaagcg 780

ccctatcaac gcgacaactt tatactacga caaactgaaa aacacgagct cctggtgcta 840

gttaagaaag atcaactgaa ccgtcactct tatctcaaag acccggactt tcttgacgcc 900

gcacttgact tcaactacct agacctcagc gcactactac gtaacagctt tcaccgttac 960

gccgtggatg tactcaagag cggtcgatgt cagatgctgg accgccgcac ggtagaaatg 1020

gccttcgcct acgcattagc actgttcgca gcagcccgac aagaagaggc cggcgcccaa 1080

gtctccgtcc cacgggccct agaccgccag gccgcactct tacaaataca agaatttatg 1140

atcacatgcc tctcacaaac accaccacgc accacgttgc tgctgtatcc cacggccgtg 1200

gacctggcca aacgagccct ttggacaccg aatcagatca ccgacatcac cagcctcgta 1260

cgcctggtct acatactctc taaacagaat cagcaacatc tcatccccca atgggcacta 1320

cgacagatcg ccgactttgc cctaaaacta cacaaaacgc acctggcctc ttttctttca 1380

gccttcgcac gccaagaact ctacctcatg ggcagcctcg tccactccat gctggtacat 1440

acgacggaga gacgcgaaat cttcatcgta gaaacgggcc tctgttcatt ggccgagcta 1500

tcacacttta cgcagttgtt agctcatcca caccacgaat acctcagcga cctgtacaca 1560

ccctgttcca gtagcgggcg acgcgatcac tcgctcgaac gcctcacgcg tctcttcccc 1620

gatgccaccg tccccgctac cgttcccgcc gccctctcca tcctatctac catgcaacca 1680

agcacgctgg aaaccttccc cgacctgttt tgcttgccgc tcggcgaatc cttctccgcg 1740

ctgaccgtct ccgaacacgt cagttatatc gtaacaaacc agtacctgat caaaggtatc 1800

tcctaccctg tctccaccac cgtcgtaggc cagagcctca tcatcaccca gacggacagt 1860

caaactaaat gcgaactgac gcgcaacatg cataccacac acagcatcac agtggcgctc 1920

aacatttcgc tagaaaactg cgccttttgc caaagcgccc tgctagaata cgacgacacg 1980

caaggcgtca tcaacatcat gtacatgcac gactcggacg acgtcctttt cgccctggat 2040

ccctacaacg aagtggtggt ctcatctccg cgaactcact acctcatgct tttgaaaaac 2100

ggtacggtac tagaagtaac tgacgtcgtc gtggacgcca ccgacagtcg tctcctcgga 2160

agcggaggct ctggtgccca tatcgtgatg gtggacgcct acaagcctac caaacatcat 2220

caccatcacc actaa 2235

<210> 13

<211> 2157

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gH with truncated transmembrane domain

<220>

<221> misc_feature

<222> (1146)..(1146)

<223> mutation C > A at position 1146

<220>

<221> misc_feature

<222> (1)..(69)

<223> Signal peptide

<220>

<221> misc_feature

<222> (70)..(2151)

<223> extracellular domain

<220>

<221> misc_feature

<222> (2152)..(2157)

<223> transmembrane domain (truncated)

<400> 13

atgcggccag gcctcccctc ctacctcatc atcctcgccg tctgtctctt cagccaccta 60

ctttcgtcac gatatggcgc agaagccgta tccgaaccgc tggacaaagc gtttcaccta 120

ctgctcaaca cctacgggag acccatccgc ttcctgcgtg aaaataccac ccagtgtacc 180

tacaacagca gcctccgtaa cagcacggtc gtcagggaaa acgccatcag tttcaacttt 240

ttccaaagct ataatcaata ctatgtattc catatgcctc gatgtctttt tgcgggtcct 300

ctggcggagc agtttctgaa ccaggtagat ctgaccgaaa ccctggaaag ataccaacag 360

agacttaaca cttacgcgct ggtatccaaa gacctggcca gctaccgatc tttttcgcag 420

cagctaaagg cacaagacag cctaggtgaa cagcccacca ctgtgccacc gcccattgac 480

ctgtcaatac ctcacgtttg gatgccaccg caaaccactc cacacggctg gacagaatca 540

cataccacct caggactaca ccgaccacac tttaaccaga cctgtatcct ctttgatgga 600

cacgatctac tattcagcac cgtcacacct tgtttgcacc aaggctttta cctcatcgac 660

gaactacgtt acgttaaaat aacactgacc gaggacttct tcgtagttac ggtgtccata 720

gacgacgaca cacccatgct gcttatcttc ggccatcttc cacgcgtact tttcaaagcg 780

ccctatcaac gcgacaactt tatactacga caaactgaaa aacacgagct cctggtgcta 840

gttaagaaag atcaactgaa ccgtcactct tatctcaaag acccggactt tcttgacgcc 900

gcacttgact tcaactacct agacctcagc gcactactac gtaacagctt tcaccgttac 960

gccgtggatg tactcaagag cggtcgatgt cagatgctgg accgccgcac ggtagaaatg 1020

gccttcgcct acgcattagc actgttcgca gcagcccgac aagaagaggc cggcgcccaa 1080

gtctccgtcc cacgggccct agaccgccag gccgcactct tacaaataca agaatttatg 1140

atcacatgcc tctcacaaac accaccacgc accacgttgc tgctgtatcc cacggccgtg 1200

gacctggcca aacgagccct ttggacaccg aatcagatca ccgacatcac cagcctcgta 1260

cgcctggtct acatactctc taaacagaat cagcaacatc tcatccccca atgggcacta 1320

cgacagatcg ccgactttgc cctaaaacta cacaaaacgc acctggcctc ttttctttca 1380

gccttcgcac gccaagaact ctacctcatg ggcagcctcg tccactccat gctggtacat 1440

acgacggaga gacgcgaaat cttcatcgta gaaacgggcc tctgttcatt ggccgagcta 1500

tcacacttta cgcagttgtt agctcatcca caccacgaat acctcagcga cctgtacaca 1560

ccctgttcca gtagcgggcg acgcgatcac tcgctcgaac gcctcacgcg tctcttcccc 1620

gatgccaccg tccccgctac cgttcccgcc gccctctcca tcctatctac catgcaacca 1680

agcacgctgg aaaccttccc cgacctgttt tgcttgccgc tcggcgaatc cttctccgcg 1740

ctgaccgtct ccgaacacgt cagttatatc gtaacaaacc agtacctgat caaaggtatc 1800

tcctaccctg tctccaccac cgtcgtaggc cagagcctca tcatcaccca gacggacagt 1860

caaactaaat gcgaactgac gcgcaacatg cataccacac acagcatcac agtggcgctc 1920

aacatttcgc tagaaaactg cgccttttgc caaagcgccc tgctagaata cgacgacacg 1980

caaggcgtca tcaacatcat gtacatgcac gactcggacg acgtcctttt cgccctggat 2040

ccctacaacg aagtggtggt ctcatctccg cgaactcact acctcatgct tttgaaaaac 2100

ggtacggtac tagaagtaac tgacgtcgtc gtggacgcca ccgacagtcg tctcctc 2157

<210> 14

<211> 18

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> linker from gH construct

<400> 14

ggaagcggag gctctggt 18

<210> 15

<211> 39

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Spytag

<400> 15

gcccatatcg tgatggtgga cgcctacaag cctaccaaa 39

<210> 16

<211> 837

<212> DNA

<213> Human cytomegalovirus (Human cytomegavirus)

<220>

<221> misc_feature

<222> (1)..(90)

<223> Signal peptide

<220>

<221> misc_feature

<222> (91)..(834)

<223> extracellular domain

<220>

<221> misc_feature

<222> (835)..(837)

<223> stop codon

<400> 16

atgtgccgcc gcccggattg cggcttctct ttctcacctg gaccggtgat actgctgtgg 60

tgttgccttc tgctgcccat tgtttcctca gccgccgtca gcgtcgctcc taccgccgcc 120

gagaaagtcc ccgcggagtg ccccgaacta acgcgccgat gcttgttggg tgaggtgttt 180

gagggtgaca agtatgaaag ttggctgcgc ccgttggtga atgttaccgg gcgcgatggc 240

ccgctatcgc aacttatccg ttaccgtccc gttacgccgg aggccgccaa ctccgtgctg 300

ttggacgagg ctttcctgga cactctggcc ctgctgtaca acaatccgga tcaattgcgg 360

gccctgctga cgctgttgag ctcggacaca gcgccgcgct ggatgacggt gatgcgcggc 420

tacagcgagt gcggcgatgg ctcgccggcc gtgtacacgt gcgtggacga cctgtgccgc 480

ggctacgacc tcacgcgact gtcatacggg cgcagcatct tcacggaaca cgtgttaggc 540

ttcgagctgg tgccaccgtc tctctttaac gtggtggtgg ccatacgcaa cgaagccacg 600

cgtaccaacc gcgccgtgcg tctgcccgtg agcaccgctg ccgcgcccga gggcatcacg 660

ctcttttacg gcctgtacaa cgcagtgaag gaattctgcc tgcgtcacca gctggacccg 720

ccgctgctac gccacctaga taaatactac gccggactgc cgcccgagct gaagcagacg 780

cgcgtcaacc tgccggctca ctcgcgctat ggccctcaag cagtggatgc tcgctaa 837

<210> 17

<211> 702

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> UL130-C tag

<220>

<221> misc_feature

<222> (1)..(75)

<223> Signal peptide

<220>

<221> misc_feature

<222> (76)..(642)

<223> extracellular domain

<220>

<221> misc_feature

<222> (643)..(687)

<223> joint

<220>

<221> misc_feature

<222> (688)..(699)

<223> C tag

<220>

<221> misc_feature

<222> (700)..(702)

<223> stop codon

<400> 17

atgctgcggc ttctgcttcg tcaccacttt cactgcctgc ttctgtgcgc ggtttgggca 60

acgccctgtc tggcgtctcc gtggtcgacg ctaacagcaa accagaatcc gtccccgcca 120

tggtctaaac tgacgtattc caaaccgcat gacgcggcga cgttttactg tccttttctc 180

tatccctcgc ccccacgatc ccccttgcaa ttctcggggt tccagcgggt atcaacgggt 240

cccgagtgtc gcaacgagac cctgtatctg ctgtacaacc gggaaggcca gaccttggtg 300

gagagaagct ccacctgggt gaaaaaggtg atctggtacc tgagcggtcg gaaccaaacc 360

atcctccaac ggatgccccg aacggcttcg aaaccgagcg acggaaacgt gcagatcagc 420

gtggaagacg ccaagatttt tggagcgcac atggtgccca agcagaccaa gctgctacgc 480

ttcgtcgtca acgatggcac acgttatcag atgtgtgtga tgaagctgga gagctgggct 540

cacgtcttcc gggactacag cgtgtctttt caggtgcgat tgacgttcac cgaggccaat 600

aaccagactt acaccttctg cacccatccc aatctcatcg ttggaggcgg aggatctggc 660

ggaggtggaa gtggcggagg cggatctgag cccgaggcct aa 702

<210> 18

<211> 642

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> UL130 (Signal sequence and extracellular Domain)

<220>

<221> misc_feature

<222> (1)..(75)

<223> Signal peptide

<220>

<221> misc_feature

<222> (76)..(642)

<223> extracellular domain

<400> 18

atgctgcggc ttctgcttcg tcaccacttt cactgcctgc ttctgtgcgc ggtttgggca 60

acgccctgtc tggcgtctcc gtggtcgacg ctaacagcaa accagaatcc gtccccgcca 120

tggtctaaac tgacgtattc caaaccgcat gacgcggcga cgttttactg tccttttctc 180

tatccctcgc ccccacgatc ccccttgcaa ttctcggggt tccagcgggt atcaacgggt 240

cccgagtgtc gcaacgagac cctgtatctg ctgtacaacc gggaaggcca gaccttggtg 300

gagagaagct ccacctgggt gaaaaaggtg atctggtacc tgagcggtcg gaaccaaacc 360

atcctccaac ggatgccccg aacggcttcg aaaccgagcg acggaaacgt gcagatcagc 420

gtggaagacg ccaagatttt tggagcgcac atggtgccca agcagaccaa gctgctacgc 480

ttcgtcgtca acgatggcac acgttatcag atgtgtgtga tgaagctgga gagctgggct 540

cacgtcttcc gggactacag cgtgtctttt caggtgcgat tgacgttcac cgaggccaat 600

aaccagactt acaccttctg cacccatccc aatctcatcg tt 642

<210> 19

<211> 45

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> linker from UL130 construct

<400> 19

ggaggcggag gatctggcgg aggtggaagt ggcggaggcg gatct 45

<210> 20

<211> 759

<212> DNA

<213> Human cytomegalovirus (Human cytomegavirus)

<220>

<221> misc_feature

<222> (634)..(634)

<223> mutation T > C at position 634

<220>

<221> misc_feature

<222> (1)..(81)

<223> Signal peptide

<220>

<221> misc_feature

<222> (82)..(164)

<223> extracellular domain

<220>

<221> Intron

<222> (165)..(287)

<220>

<221> misc_feature

<222> (288)..(422)

<223> extracellular domain

<220>

<221> Intron

<222> (423)..(542)

<220>

<221> misc_feature

<222> (543)..(756)

<223> extracellular domain

<220>

<221> misc_feature

<222> (757)..(759)

<223> stop codon

<400> 20

atgagtccca aagatctgac gccgttcttg acggcgttgt ggctgctatt gggtcacagc 60

cgcgtgccgc gggtgcgcgc agaagaatgt tgcgaattca taaacgtcaa ccacccgccg 120

gaacgctgtt acgatttcaa aatgtgcaat cgcttcaccg tcgcgtacgt attttcatga 180

ttgtctgcgt tctgtggtgc gtctggatct gtctctcgac gtttctgata gccatgttcc 240

atcgacgatc ctcgggaatg ccagagtaga ttttcatgaa tccacaggct gcggtgtccg 300

gacggcgaag tctgctacag tcccgagaaa acggctgaga ttcgcgggat cgtcaccacc 360

atgacccatt cattgacacg ccaggtcgta cacaacaaac tgacgagctg caactacaat 420

ccgtaagtct cttcctgagg gccttacagc ctatgggaga gtaagacaga gagggacaaa 480

acatcattaa aaaaaaaagt ctaatttcac gttttgtacc ccccttcccc tccgtgttgt 540

aggttatacc tcgaagctga cgggcgaata cgctgcggca aagtaaacga caaggcgcag 600

tacctgctgg gcgccgctgg cagcgttccc tatcgatgga tcaatctgga atacgacaag 660

ataacccgga tcgtgggcct ggatcagtac ctggagagcg ttaagaaaca caaacggctg 720

gatgtgtgcc gcgctaaaat gggctatatg ctgcagtga 759

<210> 21

<211> 498

<212> DNA

<213> Human cytomegalovirus (Human cytomegavirus)

<220>

<221> misc_feature

<222> (1)..(54)

<223> Signal peptide

<220>

<221> misc_feature

<222> (55)..(236)

<223> extracellular domain

<220>

<221> Intron

<222> (237)..(344)

<220>

<221> misc_feature

<222> (345)..(495)

<223> extracellular domain

<220>

<221> misc_feature

<222> (496)..(498)

<223> stop codon

<400> 21

atgcggctgt gtcgggtgtg gctgtctgtt tgtctgtgcg ccgtggtgct gggtcagtgc 60

cagcgggaaa ccgcggaaaa aaacgattat taccgagtac cgcattactg ggacgcgtgc 120

tctcgcgcgc tgcccgacca aacccgttac aagtatgtgg aacagctcgt ggacctcacg 180

ttgaactacc actacgatgc gagccacggc ttggacaact ttgacgtgct caagaggtga 240

gggtacgcgc taaagatgca tgacaacggg aaggtaaggg cgaacgggta acgggtaagt 300

aaccgcatgg ggtatgaaat gacgttcgga acctgtgctt gcagaatcaa cgtgaccgag 360

gtgtcgttgc tcatcagcga ctttagacgt cagaaccgtc gcggcggcac caacaaaagg 420

accacgttca acgccgccgg ttcgctggcg ccacacgccc ggagcctcga gttcagcgtg 480

cggctctttg ccaactag 498

<210> 22

<211> 1008

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> SpyCatcher-HBsAg

<220>

<221> misc_feature

<222> (1)..(276)

<223> SpyCatcherDeltaN1

<220>

<221> misc_feature

<222> (277)..(303)

<223> Flexible Joint

<220>

<221> misc_feature

<222> (304)..(315)

<223> PVTN joint

<220>

<221> misc_feature

<222> (316)..(993)

<223> HBsAg

<220>

<221> misc_feature

<222> (994)..(1005)

<223> C tag

<220>

<221> misc_feature

<222> (1006)..(1008)

<223> stop codon

<400> 22

gactccgcta ctcacatcaa gttctccaag agagatgagg acggtaaaga attggctggt 60

gctactatgg aattgagaga ctcctccggt aagactatct ccacttggat ttccgacggt 120

caggttaagg acttctactt gtacccaggt aagtacactt tcgttgagac tgctgctcca 180

gacggttacg aagttgctac tgctatcact ttcactgtta acgagcaggg acaggttaca 240

gttaacggta aggctactaa gggtgacgct catattggtt ctggtggatc tggtggttcc 300

ggtccagtta ctaatatgga aaacatcact tccggtttct tgggtccttt gttggttttg 360

caggctggat tcttcttgtt gactagaatc ttgactatcc cacagtcctt ggactcttgg 420

tggacttcct tgaacttctt gggtggttcc ccagtttgtt tgggtcaaaa ctctcaatcc 480

ccaacttcca accactcccc aacatcttgt ccaccaattt gtcctggtta cagatggatg 540

tgtttgagaa gattcatcat tttcttgttc atcttgttgt tgtgtttgat cttcttgttg 600

gttttgttgg actaccaggg tatgttgcca gtttgtccat tgatcccagg ttccactact 660

acaaacactg gtccatgtaa gacttgtact actccagctc agggtaactc catgttccct 720

tcatgttgtt gtactaagcc aactgacggt aactgtactt gtatcccaat tccatcctcc 780

tgggctttcg ctaagtactt gtgggaatgg gcttccgtta gattctcctg gttgtccttg 840

ttggttccat tcgttcagtg gttcgttggt ttgtccccaa ctgtttggtt gtccgctatt 900

tggatgatgt ggtactgggg tccatccttg tactctatcg tttccccatt catccctttg 960

ttgccaatct tcttctgttt gtgggtttac atcgagccag aggcttaa 1008

<210> 23

<211> 276

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> SpyCatcherDeltaN1

<400> 23

gactccgcta ctcacatcaa gttctccaag agagatgagg acggtaaaga attggctggt 60

gctactatgg aattgagaga ctcctccggt aagactatct ccacttggat ttccgacggt 120

caggttaagg acttctactt gtacccaggt aagtacactt tcgttgagac tgctgctcca 180

gacggttacg aagttgctac tgctatcact ttcactgtta acgagcaggg acaggttaca 240

gttaacggta aggctactaa gggtgacgct catatt 276

<210> 24

<211> 27

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible linker from SpyCatcher-HBsAg

<400> 24

ggttctggtg gatctggtgg ttccggt 27

<210> 25

<211> 12

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> PVTN linker from SpyCatcher-HbsAg

<400> 25

ccagttacta at 12

<210> 26

<211> 678

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> HBsAg

<400> 26

atggaaaaca tcacttccgg tttcttgggt cctttgttgg ttttgcaggc tggattcttc 60

ttgttgacta gaatcttgac tatcccacag tccttggact cttggtggac ttccttgaac 120

ttcttgggtg gttccccagt ttgtttgggt caaaactctc aatccccaac ttccaaccac 180

tccccaacat cttgtccacc aatttgtcct ggttacagat ggatgtgttt gagaagattc 240

atcattttct tgttcatctt gttgttgtgt ttgatcttct tgttggtttt gttggactac 300

cagggtatgt tgccagtttg tccattgatc ccaggttcca ctactacaaa cactggtcca 360

tgtaagactt gtactactcc agctcagggt aactccatgt tcccttcatg ttgttgtact 420

aagccaactg acggtaactg tacttgtatc ccaattccat cctcctgggc tttcgctaag 480

tacttgtggg aatgggcttc cgttagattc tcctggttgt ccttgttggt tccattcgtt 540

cagtggttcg ttggtttgtc cccaactgtt tggttgtccg ctatttggat gatgtggtac 600

tggggtccat ccttgtactc tatcgtttcc ccattcatcc ctttgttgcc aatcttcttc 660

tgtttgtggg tttacatc 678

<210> 27

<211> 744

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gH-SpyTag-His

<220>

<221> misc_feature

<222> (1)..(23)

<223> Signal peptide

<220>

<221> misc_feature

<222> (24)..(717)

<223> extracellular domain

<220>

<221> misc_feature

<222> (718)..(719)

<223> transmembrane domain (truncated)

<220>

<221> misc_feature

<222> (720)..(725)

<223> joint

<220>

<221> misc_feature

<222> (726)..(738)

<223> Spytag

<220>

<221> misc_feature

<222> (739)..(744)

<223> 6 × His tag

<400> 27

Met Arg Pro Gly Leu Pro Ser Tyr Leu Ile Ile Leu Ala Val Cys Leu

1 5 10 15

Phe Ser His Leu Leu Ser Ser Arg Tyr Gly Ala Glu Ala Val Ser Glu

20 25 30

Pro Leu Asp Lys Ala Phe His Leu Leu Leu Asn Thr Tyr Gly Arg Pro

35 40 45

Ile Arg Phe Leu Arg Glu Asn Thr Thr Gln Cys Thr Tyr Asn Ser Ser

50 55 60

Leu Arg Asn Ser Thr Val Val Arg Glu Asn Ala Ile Ser Phe Asn Phe

65 70 75 80

Phe Gln Ser Tyr Asn Gln Tyr Tyr Val Phe His Met Pro Arg Cys Leu

85 90 95

Phe Ala Gly Pro Leu Ala Glu Gln Phe Leu Asn Gln Val Asp Leu Thr

100 105 110

Glu Thr Leu Glu Arg Tyr Gln Gln Arg Leu Asn Thr Tyr Ala Leu Val

115 120 125

Ser Lys Asp Leu Ala Ser Tyr Arg Ser Phe Ser Gln Gln Leu Lys Ala

130 135 140

Gln Asp Ser Leu Gly Glu Gln Pro Thr Thr Val Pro Pro Pro Ile Asp

145 150 155 160

Leu Ser Ile Pro His Val Trp Met Pro Pro Gln Thr Thr Pro His Gly

165 170 175

Trp Thr Glu Ser His Thr Thr Ser Gly Leu His Arg Pro His Phe Asn

180 185 190

Gln Thr Cys Ile Leu Phe Asp Gly His Asp Leu Leu Phe Ser Thr Val

195 200 205

Thr Pro Cys Leu His Gln Gly Phe Tyr Leu Ile Asp Glu Leu Arg Tyr

210 215 220

Val Lys Ile Thr Leu Thr Glu Asp Phe Phe Val Val Thr Val Ser Ile

225 230 235 240

Asp Asp Asp Thr Pro Met Leu Leu Ile Phe Gly His Leu Pro Arg Val

245 250 255

Leu Phe Lys Ala Pro Tyr Gln Arg Asp Asn Phe Ile Leu Arg Gln Thr

260 265 270

Glu Lys His Glu Leu Leu Val Leu Val Lys Lys Asp Gln Leu Asn Arg

275 280 285

His Ser Tyr Leu Lys Asp Pro Asp Phe Leu Asp Ala Ala Leu Asp Phe

290 295 300

Asn Tyr Leu Asp Leu Ser Ala Leu Leu Arg Asn Ser Phe His Arg Tyr

305 310 315 320

Ala Val Asp Val Leu Lys Ser Gly Arg Cys Gln Met Leu Asp Arg Arg

325 330 335

Thr Val Glu Met Ala Phe Ala Tyr Ala Leu Ala Leu Phe Ala Ala Ala

340 345 350

Arg Gln Glu Glu Ala Gly Ala Gln Val Ser Val Pro Arg Ala Leu Asp

355 360 365

Arg Gln Ala Ala Leu Leu Gln Ile Gln Glu Phe Met Ile Thr Cys Leu

370 375 380

Ser Gln Thr Pro Pro Arg Thr Thr Leu Leu Leu Tyr Pro Thr Ala Val

385 390 395 400

Asp Leu Ala Lys Arg Ala Leu Trp Thr Pro Asn Gln Ile Thr Asp Ile

405 410 415

Thr Ser Leu Val Arg Leu Val Tyr Ile Leu Ser Lys Gln Asn Gln Gln

420 425 430

His Leu Ile Pro Gln Trp Ala Leu Arg Gln Ile Ala Asp Phe Ala Leu

435 440 445

Lys Leu His Lys Thr His Leu Ala Ser Phe Leu Ser Ala Phe Ala Arg

450 455 460

Gln Glu Leu Tyr Leu Met Gly Ser Leu Val His Ser Met Leu Val His

465 470 475 480

Thr Thr Glu Arg Arg Glu Ile Phe Ile Val Glu Thr Gly Leu Cys Ser

485 490 495

Leu Ala Glu Leu Ser His Phe Thr Gln Leu Leu Ala His Pro His His

500 505 510

Glu Tyr Leu Ser Asp Leu Tyr Thr Pro Cys Ser Ser Ser Gly Arg Arg

515 520 525

Asp His Ser Leu Glu Arg Leu Thr Arg Leu Phe Pro Asp Ala Thr Val

530 535 540

Pro Ala Thr Val Pro Ala Ala Leu Ser Ile Leu Ser Thr Met Gln Pro

545 550 555 560

Ser Thr Leu Glu Thr Phe Pro Asp Leu Phe Cys Leu Pro Leu Gly Glu

565 570 575

Ser Phe Ser Ala Leu Thr Val Ser Glu His Val Ser Tyr Ile Val Thr

580 585 590

Asn Gln Tyr Leu Ile Lys Gly Ile Ser Tyr Pro Val Ser Thr Thr Val

595 600 605

Val Gly Gln Ser Leu Ile Ile Thr Gln Thr Asp Ser Gln Thr Lys Cys

610 615 620

Glu Leu Thr Arg Asn Met His Thr Thr His Ser Ile Thr Val Ala Leu

625 630 635 640

Asn Ile Ser Leu Glu Asn Cys Ala Phe Cys Gln Ser Ala Leu Leu Glu

645 650 655

Tyr Asp Asp Thr Gln Gly Val Ile Asn Ile Met Tyr Met His Asp Ser

660 665 670

Asp Asp Val Leu Phe Ala Leu Asp Pro Tyr Asn Glu Val Val Val Ser

675 680 685

Ser Pro Arg Thr His Tyr Leu Met Leu Leu Lys Asn Gly Thr Val Leu

690 695 700

Glu Val Thr Asp Val Val Val Asp Ala Thr Asp Ser Arg Leu Leu Gly

705 710 715 720

Ser Gly Gly Ser Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro

725 730 735

Thr Lys His His His His His His

740

<210> 28

<211> 719

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gH with truncated transmembrane domain

<220>

<221> misc_feature

<222> (1)..(23)

<223> Signal peptide

<220>

<221> misc_feature

<222> (24)..(717)

<223> extracellular domain

<220>

<221> misc_feature

<222> (718)..(719)

<223> transmembrane domain (truncated)

<400> 28

Met Arg Pro Gly Leu Pro Ser Tyr Leu Ile Ile Leu Ala Val Cys Leu

1 5 10 15

Phe Ser His Leu Leu Ser Ser Arg Tyr Gly Ala Glu Ala Val Ser Glu

20 25 30

Pro Leu Asp Lys Ala Phe His Leu Leu Leu Asn Thr Tyr Gly Arg Pro

35 40 45

Ile Arg Phe Leu Arg Glu Asn Thr Thr Gln Cys Thr Tyr Asn Ser Ser

50 55 60

Leu Arg Asn Ser Thr Val Val Arg Glu Asn Ala Ile Ser Phe Asn Phe

65 70 75 80

Phe Gln Ser Tyr Asn Gln Tyr Tyr Val Phe His Met Pro Arg Cys Leu

85 90 95

Phe Ala Gly Pro Leu Ala Glu Gln Phe Leu Asn Gln Val Asp Leu Thr

100 105 110

Glu Thr Leu Glu Arg Tyr Gln Gln Arg Leu Asn Thr Tyr Ala Leu Val

115 120 125

Ser Lys Asp Leu Ala Ser Tyr Arg Ser Phe Ser Gln Gln Leu Lys Ala

130 135 140

Gln Asp Ser Leu Gly Glu Gln Pro Thr Thr Val Pro Pro Pro Ile Asp

145 150 155 160

Leu Ser Ile Pro His Val Trp Met Pro Pro Gln Thr Thr Pro His Gly

165 170 175

Trp Thr Glu Ser His Thr Thr Ser Gly Leu His Arg Pro His Phe Asn

180 185 190

Gln Thr Cys Ile Leu Phe Asp Gly His Asp Leu Leu Phe Ser Thr Val

195 200 205

Thr Pro Cys Leu His Gln Gly Phe Tyr Leu Ile Asp Glu Leu Arg Tyr

210 215 220

Val Lys Ile Thr Leu Thr Glu Asp Phe Phe Val Val Thr Val Ser Ile

225 230 235 240

Asp Asp Asp Thr Pro Met Leu Leu Ile Phe Gly His Leu Pro Arg Val

245 250 255

Leu Phe Lys Ala Pro Tyr Gln Arg Asp Asn Phe Ile Leu Arg Gln Thr

260 265 270

Glu Lys His Glu Leu Leu Val Leu Val Lys Lys Asp Gln Leu Asn Arg

275 280 285

His Ser Tyr Leu Lys Asp Pro Asp Phe Leu Asp Ala Ala Leu Asp Phe

290 295 300

Asn Tyr Leu Asp Leu Ser Ala Leu Leu Arg Asn Ser Phe His Arg Tyr

305 310 315 320

Ala Val Asp Val Leu Lys Ser Gly Arg Cys Gln Met Leu Asp Arg Arg

325 330 335

Thr Val Glu Met Ala Phe Ala Tyr Ala Leu Ala Leu Phe Ala Ala Ala

340 345 350

Arg Gln Glu Glu Ala Gly Ala Gln Val Ser Val Pro Arg Ala Leu Asp

355 360 365

Arg Gln Ala Ala Leu Leu Gln Ile Gln Glu Phe Met Ile Thr Cys Leu

370 375 380

Ser Gln Thr Pro Pro Arg Thr Thr Leu Leu Leu Tyr Pro Thr Ala Val

385 390 395 400

Asp Leu Ala Lys Arg Ala Leu Trp Thr Pro Asn Gln Ile Thr Asp Ile

405 410 415

Thr Ser Leu Val Arg Leu Val Tyr Ile Leu Ser Lys Gln Asn Gln Gln

420 425 430

His Leu Ile Pro Gln Trp Ala Leu Arg Gln Ile Ala Asp Phe Ala Leu

435 440 445

Lys Leu His Lys Thr His Leu Ala Ser Phe Leu Ser Ala Phe Ala Arg

450 455 460

Gln Glu Leu Tyr Leu Met Gly Ser Leu Val His Ser Met Leu Val His

465 470 475 480

Thr Thr Glu Arg Arg Glu Ile Phe Ile Val Glu Thr Gly Leu Cys Ser

485 490 495

Leu Ala Glu Leu Ser His Phe Thr Gln Leu Leu Ala His Pro His His

500 505 510

Glu Tyr Leu Ser Asp Leu Tyr Thr Pro Cys Ser Ser Ser Gly Arg Arg

515 520 525

Asp His Ser Leu Glu Arg Leu Thr Arg Leu Phe Pro Asp Ala Thr Val

530 535 540

Pro Ala Thr Val Pro Ala Ala Leu Ser Ile Leu Ser Thr Met Gln Pro

545 550 555 560

Ser Thr Leu Glu Thr Phe Pro Asp Leu Phe Cys Leu Pro Leu Gly Glu

565 570 575

Ser Phe Ser Ala Leu Thr Val Ser Glu His Val Ser Tyr Ile Val Thr

580 585 590

Asn Gln Tyr Leu Ile Lys Gly Ile Ser Tyr Pro Val Ser Thr Thr Val

595 600 605

Val Gly Gln Ser Leu Ile Ile Thr Gln Thr Asp Ser Gln Thr Lys Cys

610 615 620

Glu Leu Thr Arg Asn Met His Thr Thr His Ser Ile Thr Val Ala Leu

625 630 635 640

Asn Ile Ser Leu Glu Asn Cys Ala Phe Cys Gln Ser Ala Leu Leu Glu

645 650 655

Tyr Asp Asp Thr Gln Gly Val Ile Asn Ile Met Tyr Met His Asp Ser

660 665 670

Asp Asp Val Leu Phe Ala Leu Asp Pro Tyr Asn Glu Val Val Val Ser

675 680 685

Ser Pro Arg Thr His Tyr Leu Met Leu Leu Lys Asn Gly Thr Val Leu

690 695 700

Glu Val Thr Asp Val Val Val Asp Ala Thr Asp Ser Arg Leu Leu

705 710 715

<210> 29

<211> 6

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> linker from gH construct

<400> 29

Gly Ser Gly Gly Ser Gly

1 5

<210> 30

<211> 13

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Spytag

<400> 30

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys

1 5 10

<210> 31

<211> 278

<212> PRT

<213> Human cytomegalovirus (Human cytomegavirus)

<220>

<221> misc_feature

<222> (1)..(30)

<223> Signal peptide

<220>

<221> misc_feature

<222> (31)..(278)

<223> extracellular domain

<400> 31

Met Cys Arg Arg Pro Asp Cys Gly Phe Ser Phe Ser Pro Gly Pro Val

1 5 10 15

Ile Leu Leu Trp Cys Cys Leu Leu Leu Pro Ile Val Ser Ser Ala Ala

20 25 30

Val Ser Val Ala Pro Thr Ala Ala Glu Lys Val Pro Ala Glu Cys Pro

35 40 45

Glu Leu Thr Arg Arg Cys Leu Leu Gly Glu Val Phe Glu Gly Asp Lys

50 55 60

Tyr Glu Ser Trp Leu Arg Pro Leu Val Asn Val Thr Gly Arg Asp Gly

65 70 75 80

Pro Leu Ser Gln Leu Ile Arg Tyr Arg Pro Val Thr Pro Glu Ala Ala

85 90 95

Asn Ser Val Leu Leu Asp Glu Ala Phe Leu Asp Thr Leu Ala Leu Leu

100 105 110

Tyr Asn Asn Pro Asp Gln Leu Arg Ala Leu Leu Thr Leu Leu Ser Ser

115 120 125

Asp Thr Ala Pro Arg Trp Met Thr Val Met Arg Gly Tyr Ser Glu Cys

130 135 140

Gly Asp Gly Ser Pro Ala Val Tyr Thr Cys Val Asp Asp Leu Cys Arg

145 150 155 160

Gly Tyr Asp Leu Thr Arg Leu Ser Tyr Gly Arg Ser Ile Phe Thr Glu

165 170 175

His Val Leu Gly Phe Glu Leu Val Pro Pro Ser Leu Phe Asn Val Val

180 185 190

Val Ala Ile Arg Asn Glu Ala Thr Arg Thr Asn Arg Ala Val Arg Leu

195 200 205

Pro Val Ser Thr Ala Ala Ala Pro Glu Gly Ile Thr Leu Phe Tyr Gly

210 215 220

Leu Tyr Asn Ala Val Lys Glu Phe Cys Leu Arg His Gln Leu Asp Pro

225 230 235 240

Pro Leu Leu Arg His Leu Asp Lys Tyr Tyr Ala Gly Leu Pro Pro Glu

245 250 255

Leu Lys Gln Thr Arg Val Asn Leu Pro Ala His Ser Arg Tyr Gly Pro

260 265 270

Gln Ala Val Asp Ala Arg

275

<210> 32

<211> 233

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> UL130-C tag

<220>

<221> misc_feature

<222> (1)..(25)

<223> Signal peptide

<220>

<221> misc_feature

<222> (26)..(214)

<223> extracellular domain

<220>

<221> misc_feature

<222> (215)..(229)

<223> joint

<220>

<221> misc_feature

<222> (230)..(233)

<223> C tag

<400> 32

Met Leu Arg Leu Leu Leu Arg His His Phe His Cys Leu Leu Leu Cys

1 5 10 15

Ala Val Trp Ala Thr Pro Cys Leu Ala Ser Pro Trp Ser Thr Leu Thr

20 25 30

Ala Asn Gln Asn Pro Ser Pro Pro Trp Ser Lys Leu Thr Tyr Ser Lys

35 40 45

Pro His Asp Ala Ala Thr Phe Tyr Cys Pro Phe Leu Tyr Pro Ser Pro

50 55 60

Pro Arg Ser Pro Leu Gln Phe Ser Gly Phe Gln Arg Val Ser Thr Gly

65 70 75 80

Pro Glu Cys Arg Asn Glu Thr Leu Tyr Leu Leu Tyr Asn Arg Glu Gly

85 90 95

Gln Thr Leu Val Glu Arg Ser Ser Thr Trp Val Lys Lys Val Ile Trp

100 105 110

Tyr Leu Ser Gly Arg Asn Gln Thr Ile Leu Gln Arg Met Pro Arg Thr

115 120 125

Ala Ser Lys Pro Ser Asp Gly Asn Val Gln Ile Ser Val Glu Asp Ala

130 135 140

Lys Ile Phe Gly Ala His Met Val Pro Lys Gln Thr Lys Leu Leu Arg

145 150 155 160

Phe Val Val Asn Asp Gly Thr Arg Tyr Gln Met Cys Val Met Lys Leu

165 170 175

Glu Ser Trp Ala His Val Phe Arg Asp Tyr Ser Val Ser Phe Gln Val

180 185 190

Arg Leu Thr Phe Thr Glu Ala Asn Asn Gln Thr Tyr Thr Phe Cys Thr

195 200 205

His Pro Asn Leu Ile Val Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser

210 215 220

Gly Gly Gly Gly Ser Glu Pro Glu Ala

225 230

<210> 33

<211> 214

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> UL130 (Signal sequence and extracellular Domain)

<220>

<221> misc_feature

<222> (1)..(25)

<223> Signal peptide

<220>

<221> misc_feature

<222> (26)..(214)

<223> extracellular domain

<400> 33

Met Leu Arg Leu Leu Leu Arg His His Phe His Cys Leu Leu Leu Cys

1 5 10 15

Ala Val Trp Ala Thr Pro Cys Leu Ala Ser Pro Trp Ser Thr Leu Thr

20 25 30

Ala Asn Gln Asn Pro Ser Pro Pro Trp Ser Lys Leu Thr Tyr Ser Lys

35 40 45

Pro His Asp Ala Ala Thr Phe Tyr Cys Pro Phe Leu Tyr Pro Ser Pro

50 55 60

Pro Arg Ser Pro Leu Gln Phe Ser Gly Phe Gln Arg Val Ser Thr Gly

65 70 75 80

Pro Glu Cys Arg Asn Glu Thr Leu Tyr Leu Leu Tyr Asn Arg Glu Gly

85 90 95

Gln Thr Leu Val Glu Arg Ser Ser Thr Trp Val Lys Lys Val Ile Trp

100 105 110

Tyr Leu Ser Gly Arg Asn Gln Thr Ile Leu Gln Arg Met Pro Arg Thr

115 120 125

Ala Ser Lys Pro Ser Asp Gly Asn Val Gln Ile Ser Val Glu Asp Ala

130 135 140

Lys Ile Phe Gly Ala His Met Val Pro Lys Gln Thr Lys Leu Leu Arg

145 150 155 160

Phe Val Val Asn Asp Gly Thr Arg Tyr Gln Met Cys Val Met Lys Leu

165 170 175

Glu Ser Trp Ala His Val Phe Arg Asp Tyr Ser Val Ser Phe Gln Val

180 185 190

Arg Leu Thr Phe Thr Glu Ala Asn Asn Gln Thr Tyr Thr Phe Cys Thr

195 200 205

His Pro Asn Leu Ile Val

210

<210> 34

<211> 15

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> linker from UL130 construct

<400> 34

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser

1 5 10 15

<210> 35

<211> 171

<212> PRT

<213> Human cytomegalovirus (Human cytomegavirus)

<220>

<221> misc_feature

<222> (1)..(27)

<223> Signal peptide

<220>

<221> misc_feature

<222> (28)..(171)

<223> extracellular domain

<400> 35

Met Ser Pro Lys Asp Leu Thr Pro Phe Leu Thr Ala Leu Trp Leu Leu

1 5 10 15

Leu Gly His Ser Arg Val Pro Arg Val Arg Ala Glu Glu Cys Cys Glu

20 25 30

Phe Ile Asn Val Asn His Pro Pro Glu Arg Cys Tyr Asp Phe Lys Met

35 40 45

Cys Asn Arg Phe Thr Val Ala Leu Arg Cys Pro Asp Gly Glu Val Cys

50 55 60

Tyr Ser Pro Glu Lys Thr Ala Glu Ile Arg Gly Ile Val Thr Thr Met

65 70 75 80

Thr His Ser Leu Thr Arg Gln Val Val His Asn Lys Leu Thr Ser Cys

85 90 95

Asn Tyr Asn Pro Leu Tyr Leu Glu Ala Asp Gly Arg Ile Arg Cys Gly

100 105 110

Lys Val Asn Asp Lys Ala Gln Tyr Leu Leu Gly Ala Ala Gly Ser Val

115 120 125

Pro Tyr Arg Trp Ile Asn Leu Glu Tyr Asp Lys Ile Thr Arg Ile Val

130 135 140

Gly Leu Asp Gln Tyr Leu Glu Ser Val Lys Lys His Lys Arg Leu Asp

145 150 155 160

Val Cys Arg Ala Lys Met Gly Tyr Met Leu Gln

165 170

<210> 36

<211> 129

<212> PRT

<213> Human cytomegalovirus (Human cytomegavirus)

<220>

<221> misc_feature

<222> (1)..(18)

<223> Signal peptide

<220>

<221> misc_feature

<222> (19)..(129)

<223> extracellular domain

<400> 36

Met Arg Leu Cys Arg Val Trp Leu Ser Val Cys Leu Cys Ala Val Val

1 5 10 15

Leu Gly Gln Cys Gln Arg Glu Thr Ala Glu Lys Asn Asp Tyr Tyr Arg

20 25 30

Val Pro His Tyr Trp Asp Ala Cys Ser Arg Ala Leu Pro Asp Gln Thr

35 40 45

Arg Tyr Lys Tyr Val Glu Gln Leu Val Asp Leu Thr Leu Asn Tyr His

50 55 60

Tyr Asp Ala Ser His Gly Leu Asp Asn Phe Asp Val Leu Lys Arg Ile

65 70 75 80

Asn Val Thr Glu Val Ser Leu Leu Ile Ser Asp Phe Arg Arg Gln Asn

85 90 95

Arg Arg Gly Gly Thr Asn Lys Arg Thr Thr Phe Asn Ala Ala Gly Ser

100 105 110

Leu Ala Pro His Ala Arg Ser Leu Glu Phe Ser Val Arg Leu Phe Ala

115 120 125

Asn

<210> 37

<211> 335

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> SpyCatcher-HBsAg

<220>

<221> misc_feature

<222> (1)..(92)

<223> SpycatcherDeltaN1

<220>

<221> misc_feature

<222> (93)..(101)

<223> Flexible Joint

<220>

<221> misc_feature

<222> (102)..(105)

<223> PVTN joint

<220>

<221> misc_feature

<222> (106)..(331)

<223> HBsAg

<220>

<221> misc_feature

<222> (332)..(335)

<223> C tag

<400> 37

Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys

1 5 10 15

Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr

20 25 30

Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr

35 40 45

Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu

50 55 60

Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr

65 70 75 80

Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Gly Ser Gly Gly

85 90 95

Ser Gly Gly Ser Gly Pro Val Thr Asn Met Glu Asn Ile Thr Ser Gly

100 105 110

Phe Leu Gly Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu Thr

115 120 125

Arg Ile Leu Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser Leu

130 135 140

Asn Phe Leu Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln Ser

145 150 155 160

Pro Thr Ser Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro Gly

165 170 175

Tyr Arg Trp Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile Leu

180 185 190

Leu Leu Cys Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly Met

195 200 205

Leu Pro Val Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr Gly

210 215 220

Pro Cys Lys Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe Pro

225 230 235 240

Ser Cys Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile Pro

245 250 255

Ile Pro Ser Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala Ser

260 265 270

Val Arg Phe Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp Phe

275 280 285

Val Gly Leu Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met Trp

290 295 300

Tyr Trp Gly Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro Leu

305 310 315 320

Leu Pro Ile Phe Phe Cys Leu Trp Val Tyr Ile Glu Pro Glu Ala

325 330 335

<210> 38

<211> 92

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> SpyCatcherDeltaN1

<400> 38

Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys

1 5 10 15

Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr

20 25 30

Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr

35 40 45

Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu

50 55 60

Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr

65 70 75 80

Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile

85 90

<210> 39

<211> 9

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Flexible linker from SpyCatcher-HBsAg

<400> 39

Gly Ser Gly Gly Ser Gly Gly Ser Gly

1 5

<210> 40

<211> 4

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> PVTN linker from SpyCatcher-HBsAg

<400> 40

Pro Val Thr Asn

1

<210> 41

<211> 226

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> HBsAg

<400> 41

Met Glu Asn Ile Thr Ser Gly Phe Leu Gly Pro Leu Leu Val Leu Gln

1 5 10 15

Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu Thr Ile Pro Gln Ser Leu

20 25 30

Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu Gly Gly Ser Pro Val Cys

35 40 45

Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser Asn His Ser Pro Thr Ser

50 55 60

Cys Pro Pro Ile Cys Pro Gly Tyr Arg Trp Met Cys Leu Arg Arg Phe

65 70 75 80

Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys Leu Ile Phe Leu Leu Val

85 90 95

Leu Leu Asp Tyr Gln Gly Met Leu Pro Val Cys Pro Leu Ile Pro Gly

100 105 110

Ser Thr Thr Thr Asn Thr Gly Pro Cys Lys Thr Cys Thr Thr Pro Ala

115 120 125

Gln Gly Asn Ser Met Phe Pro Ser Cys Cys Cys Thr Lys Pro Thr Asp

130 135 140

Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser Ser Trp Ala Phe Ala Lys

145 150 155 160

Tyr Leu Trp Glu Trp Ala Ser Val Arg Phe Ser Trp Leu Ser Leu Leu

165 170 175

Val Pro Phe Val Gln Trp Phe Val Gly Leu Ser Pro Thr Val Trp Leu

180 185 190

Ser Ala Ile Trp Met Met Trp Tyr Trp Gly Pro Ser Leu Tyr Ser Ile

195 200 205

Val Ser Pro Phe Ile Pro Leu Leu Pro Ile Phe Phe Cys Leu Trp Val

210 215 220

Tyr Ile

225

<210> 42

<211> 2235

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gH- (GSG)2-SpyTag-His (without intron) optimized for CHO expression

<220>

<221> misc_feature

<222> (1)..(69)

<223> gH Signal peptide

<220>

<221> misc_feature

<222> (70)..(2151)

<223> gH extracellular domain

<220>

<221> misc_feature

<222> (2152)..(2157)

<223> gH truncated transmembrane domain

<220>

<221> misc_feature

<222> (2158)..(2175)

<223> (GSG)2 linker

<220>

<221> misc_feature

<222> (2176)..(2214)

<223> Spytag

<220>

<221> misc_feature

<222> (2215)..(2232)

<223> Histag

<220>

<221> misc_feature

<222> (2233)..(2235)

<223> stop codon

<400> 42

atgaggcctg gcctgccttc ttatctgatc atcctggccg tgtgcctgtt ctcccatctg 60

ctgtcctcta gatacggcgc cgaggctgtg tctgagcctc tggataaggc ctttcatctg 120

ctgctgaaca cctacggcag acctatccgg ttcctgcgcg agaacaccac acagtgcacc 180

tacaactcca gcctgcggaa ctccacagtc gtgcgggaaa acgccatctc cttcaacttt 240

ttccagtcct acaaccagta ctatgtgttc cacatgcctc gctgcctgtt cgctggacct 300

ctggctgagc agttcctgaa ccaggtggac ctgaccgaga cactggaaag ataccagcag 360

cggctgaaca catatgccct ggtgtctaag gacctggcct cctacagatc cttcagccag 420

cagctgaagg ctcaggactc tctgggagag cagcctacaa cagtgcctcc tcctatcgac 480

ctgtctatcc ctcacgtgtg gatgcctcca cagaccacac ctcatggctg gaccgagtct 540

cataccacct ctggcctgca ccggcctcac ttcaaccaga cctgcatcct gttcgacggc 600

cacgacctgc tgttctccac cgtgacacca tgtctgcacc agggcttcta cctgatcgac 660

gagctgagat acgtgaagat taccctgaca gaggacttct tcgtggtcac cgtgtccatc 720

gacgacgaca cccctatgct gctgatcttc ggccatctgc ctcgggtgct gttcaaggcc 780

ccttaccagc gggacaactt catcctgaga cagaccgaga agcacgagct gctggtgctg 840

gtcaagaagg accagctgaa ccggcactcc tacctgaagg accctgactt cctggacgcc 900

gctctggact tcaactacct ggatctgagc gccctgctgc ggaacagctt tcacagatac 960

gccgtggacg tgctgaagtc tggcagatgc cagatgctgg acagacggac cgtggaaatg 1020

gccttcgctt acgccctggc tctgtttgcc gccgctagac aagaagaggc tggcgcccaa 1080

gtgtccgtgc ctagagcact ggatagacaa gccgctctgc tgcagatcca agagttcatg 1140

atcacatgcc tgtctcagac ccctcctcgg accacactgc tgctgtatcc taccgctgtg 1200

gatctggcca agagggctct gtggacccct aaccagatca ccgacatcac atccctcgtg 1260

cggctggtgt acatcctgtc caagcagaac cagcagcatc tgatccctca gtgggccctg 1320

aggcagatcg ctgattttgc cctgaagctg cacaagaccc acctggccag ctttctgtct 1380

gccttcgcca gacaagagct gtacctgatg ggcagcctgg tgcactctat gctggtgcat 1440

accaccgagc ggcgcgagat cttcatcgtg gaaaccggcc tgtgttccct ggccgagctg 1500

tctcacttta cccagctgct cgctcaccct caccacgagt acctgtccga cctgtacacc 1560

ccttgctcct ctagcggcag aagggaccac agcctggaaa gactgacccg gctgttccct 1620

gatgccaccg tgcctgctac agttcctgcc gctctgtcca tcctgagcac catgcagcct 1680

tccactctgg aaacattccc cgacctgttc tgcctgcctc tgggcgagtc tttttctgcc 1740

ctgaccgtgt ccgagcacgt gtcctacatc gtgaccaatc agtacctgat caagggcatc 1800

agctaccccg tgtccacaac cgtcgttggc cagagcctga tcatcaccca gaccgactct 1860

cagaccaagt gcgagctgac ccggaacatg cacacaaccc actccatcac cgtggctctg 1920

aacatctccc tggaaaactg cgccttctgc cagtctgccc tgctggaata cgatgacacc 1980

cagggcgtga tcaacatcat gtatatgcac gactccgacg acgtgctgtt tgccctggat 2040

ccttacaacg aggtggtggt gtctagcccc agaacacact acctgatgct gctcaagaac 2100

ggcaccgtgc tggaagtgac cgacgtggtg gtggacgcca ccgattctag attgctcggc 2160

tctggtggct ccggcgctca tatcgtgatg gtggatgctt acaagcccac caagcaccat 2220

catcaccacc actaa 2235

<210> 43

<211> 837

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gL (without intron) optimized for CHO expression

<220>

<221> misc_feature

<222> (1)..(90)

<223> gL Signal peptide

<220>

<221> misc_feature

<222> (91)..(834)

<223> gL extracellular domain

<220>

<221> misc_feature

<222> (835)..(837)

<223> stop codon

<400> 43

atgtgcagaa ggcctgactg cggcttctcc ttctctcccg gacctgtgat cctgctgtgg 60

tgctgtctgc tgctgcccat cgtttcttcc gccgctgtgt ctgtggctcc taccgctgct 120

gaaaaggtgc cagctgagtg tcccgagctg accagaagat gtctgctggg cgaagtgttc 180

gagggcgata agtacgagtc ttggctgcgg cctctggtca acgtgaccgg aagagatgga 240

cccctgagcc agctgatccg gtacagacct gtgacacctg aggccgccaa ttccgtgctg 300

ctggatgagg ccttcctgga cacactggcc ctgctgtaca acaaccccga tcagctgaga 360

gccctgctga ccctgctgtc ctctgatacc gctcctagat ggatgaccgt gatgcggggc 420

tactctgagt gcggagatgg aagcccagcc gtgtacacct gtgtggacga tctgtgcaga 480

ggctacgacc tgaccagact gtcctacggc cggtccatct ttaccgagca tgtgctgggc 540

tttgagctgg tgcctcctag cctgttcaat gtggtggtgg ccatccggaa tgaggccacc 600

agaacaaata gagccgtgcg gctgcctgtg tctacagctg ctgctcctga gggcatcacc 660

ctgttctacg gcctgtacaa cgccgtgaaa gagttctgcc tgagacacca gctggaccct 720

ccactgctga ggcacctgga taagtactac gctggcctgc ctcctgagct gaagcagacc 780

agagtgaacc tgcctgctca ctccagatac ggccctcagg ctgtggacgc cagataa 837

<210> 44

<211> 516

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> UL128 (without intron) optimized for CHO expression

<220>

<221> misc_feature

<222> (1)..(81)

<223> UL128 signal peptide

<220>

<221> misc_feature

<222> (82)..(513)

<223> UL128 extracellular domain

<220>

<221> misc_feature

<222> (514)..(516)

<223> stop codon

<400> 44

atgtccccta aggatctgac ccctttcctg accgctctgt ggctgctgct gggccattct 60

agagtgccta gagtcagagc cgaggaatgc tgcgagttca tcaacgtgaa ccatcctcca 120

gagcggtgct acgacttcaa gatgtgcaac agattcaccg tggctctgcg gtgccctgat 180

ggcgaagtgt gctactcccc tgaaaagacc gccgagatca gaggcatcgt gaccaccatg 240

acacactccc tgaccagaca ggtggtgcac aacaagctga ccagctgcaa ctacaaccct 300

ctgtacctgg aagccgacgg cagaatcaga tgcggcaaag tgaacgacaa ggcccagtac 360

ctgttgggcg ctgctggctc tgtgccctac agatggatca acctggaata cgacaagatc 420

acccggatcg tcggcctgga ccagtatctg gaatccgtga agaagcacaa gcggctggac 480

gtgtgcagag ccaagatggg ctatatgctg cagtaa 516

<210> 45

<211> 702

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> UL130- (G4S)3-C tag (without intron) optimized for CHO expression

<220>

<221> misc_feature

<222> (1)..(75)

<223> UL130 signal peptide

<220>

<221> misc_feature

<222> (76)..(642)

<223> UL130 ectodomain

<220>

<221> misc_feature

<222> (643)..(687)

<223> (G4S)3 Joint

<220>

<221> misc_feature

<222> (688)..(699)

<223> C tag

<220>

<221> misc_feature

<222> (700)..(702)

<223> stop codon

<400> 45

atgctgagac tgctgctgag acaccacttc cactgcctgc tgctgtgtgc cgtttgggct 60

acaccttgtc tggcctctcc atggtctacc ctgaccgcca accagaatcc ttctccacct 120

tggtccaagc tgacctactc caagcctcac gatgccgcta ccttctactg cccctttctg 180

tacccatctc cacctcggag ccctctgcag ttctctggct tccagagagt gtccaccgga 240

cctgagtgcc ggaacgagac actgtacctg ctgtacaacc gcgagggcca gacactggtg 300

gaaagatcct ctacctgggt caagaaagtg atctggtatc tgagcggccg gaaccagacc 360

atcctgcaga gaatgcctcg gaccgcctct aagccttctg acggcaacgt gcagatctcc 420

gtggaagatg ccaagatctt cggcgcccac atggtgccca agcagaccaa actgctgaga 480

ttcgtggtca acgacggcac ccgctaccag atgtgcgtga tgaagctgga aagctgggcc 540

cacgtgttcc gggattactc cgtgtctttc caagtgcggc tgaccttcac cgaggccaac 600

aaccagacct acaccttctg cacccatcct aacctgatcg tcggaggcgg aggatctggc 660

ggaggtggaa gtggcggagg cggatctgag cccgaggcct aa 702

<210> 46

<211> 390

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> UL131A optimized for CHO expression (without introns)

<220>

<221> misc_feature

<222> (1)..(54)

<223> UL131 signal peptide

<220>

<221> misc_feature

<222> (55)..(387)

<223> UL131 ectodomain

<220>

<221> misc_feature

<222> (388)..(390)

<223> stop codon

<400> 46

atgagactgt gcagagtgtg gctgtccgtg tgcctgtgtg ctgtggttct gggccagtgc 60

cagagagaga cagccgagaa gaacgactac tacagagtgc cccactactg ggacgcctgc 120

agtagagctt tgcccgatca gacccggtac aaatacgtgg aacagctggt ggatctgacc 180

ctgaactacc actacgacgc ctctcacggc ctggacaact tcgacgtgct gaagcggatc 240

aacgtgaccg aggtgtccct gctgatctct gacttccggc ggcagaatag aagaggcggc 300

accaacaagc ggaccacctt taatgctgcc ggctctctgg ctccccacgc cagatctctg 360

gaattttccg tgcggctgtt cgccaactaa 390

<210> 47

<211> 1587

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F-SpyTag-C tag

<220>

<221> misc_feature

<222> (1)..(75)

<223> Signal peptide

<220>

<221> misc_feature

<222> (1435)..(1515)

<223> Foldon Domain

<220>

<221> misc_feature

<222> (1516)..(1533)

<223> (GSG)2 linker

<220>

<221> misc_feature

<222> (1534)..(1572)

<223> Spytag

<220>

<221> misc_feature

<222> (1573)..(1584)

<223> C tag

<220>

<221> misc_feature

<222> (1585)..(1587)

<223> stop codon

<400> 47

atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc 60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg 120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag 180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag 240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc 300

cccgccaccg gatctggcag cgccatttgc agcggcgtgg ccgtgtgtaa agtgctgcac 360

ctggaaggcg aagtgaacaa gatcaagtcc gccctgctgt ccaccaacaa ggccgtggtg 420

tccctgagca acggcgtgag cgtgctgacc ttcaaggtgc tggatctgaa gaactacatc 480

gacaagcagc tgctgcccat cctgaacaag cagagctgca gcatcagcaa catcgagaca 540

gtgatcgagt tccagcagaa gaacaaccgg ctgctggaaa tcacccggga gttcagcgtg 600

aacgccggag tgaccacccc cgtgtccacc tacatgctga ccaacagcga gctgctgtcc 660

ctgatcaatg acatgcccat caccaacgac cagaaaaagc tgatgagcaa caacgtgcag 720

atcgtgcggc agcagagcta ctccatcatg tgcatcatca aagaagaggt gctggcctac 780

gtggtgcagc tgcccctgta cggcgtgatc gacaccccct gctggaagct gcacaccagc 840

cccctgtgca caaccaacac caaagagggc agcaacatct gcctgacccg gaccgaccgg 900

ggctggtact gcgacaacgc cggcagcgtg tccttctttc cacaggccga gacatgcaag 960

gtgcagagca accgggtgtt ctgcgacacc atgaacagcc ggaccctgcc ctccgaagtg 1020

aacctgtgca acgtggacat cttcaacccc aagtacgact gcaagatcat gacctccaag 1080

accgacgtgt ccagctccgt gatcacctcc ctgggcgcca tcgtgtcctg ctacggcaag 1140

accaagtgca ccgccagcaa caagaacaga ggcatcatca agaccttcag caacggctgc 1200

gactacgtgt ccaataaggg cgtggacacc gtgtccgtgg gcaacacact gtactgcgtg 1260

aataagcagg aaggcaagag cctgtacgtg aagggcgagc ccatcatcaa cttctacgac 1320

cccctggtgt tccccagcga cgagttcgac gctagcatca gccaggtgaa cgagaagatc 1380

aaccagagcc tggccttcat cagaaagagc gacgaactgc tgtccgccat cggcggctac 1440

atccccgagg cccccagaga tggccaggcc tacgtgcgga aggacggcga gtgggtgctg 1500

ctgtctacat ttctgggaag cggaggctct ggtgcccata tcgtgatggt ggacgcctac 1560

aagcctacca aagagcccga ggcctaa 1587

<210> 48

<211> 1515

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Sc9-10 DS-Cav1 A149C Y458C (RSV-F)

<220>

<221> misc_feature

<222> (1)..(75)

<223> Signal peptide

<220>

<221> misc_feature

<222> (1435)..(1515)

<223> Foldon Domain

<400> 48

atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc 60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg 120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag 180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag 240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc 300

cccgccaccg gatctggcag cgccatttgc agcggcgtgg ccgtgtgtaa agtgctgcac 360

ctggaaggcg aagtgaacaa gatcaagtcc gccctgctgt ccaccaacaa ggccgtggtg 420

tccctgagca acggcgtgag cgtgctgacc ttcaaggtgc tggatctgaa gaactacatc 480

gacaagcagc tgctgcccat cctgaacaag cagagctgca gcatcagcaa catcgagaca 540

gtgatcgagt tccagcagaa gaacaaccgg ctgctggaaa tcacccggga gttcagcgtg 600

aacgccggag tgaccacccc cgtgtccacc tacatgctga ccaacagcga gctgctgtcc 660

ctgatcaatg acatgcccat caccaacgac cagaaaaagc tgatgagcaa caacgtgcag 720

atcgtgcggc agcagagcta ctccatcatg tgcatcatca aagaagaggt gctggcctac 780

gtggtgcagc tgcccctgta cggcgtgatc gacaccccct gctggaagct gcacaccagc 840

cccctgtgca caaccaacac caaagagggc agcaacatct gcctgacccg gaccgaccgg 900

ggctggtact gcgacaacgc cggcagcgtg tccttctttc cacaggccga gacatgcaag 960

gtgcagagca accgggtgtt ctgcgacacc atgaacagcc ggaccctgcc ctccgaagtg 1020

aacctgtgca acgtggacat cttcaacccc aagtacgact gcaagatcat gacctccaag 1080

accgacgtgt ccagctccgt gatcacctcc ctgggcgcca tcgtgtcctg ctacggcaag 1140

accaagtgca ccgccagcaa caagaacaga ggcatcatca agaccttcag caacggctgc 1200

gactacgtgt ccaataaggg cgtggacacc gtgtccgtgg gcaacacact gtactgcgtg 1260

aataagcagg aaggcaagag cctgtacgtg aagggcgagc ccatcatcaa cttctacgac 1320

cccctggtgt tccccagcga cgagttcgac gctagcatca gccaggtgaa cgagaagatc 1380

aaccagagcc tggccttcat cagaaagagc gacgaactgc tgtccgccat cggcggctac 1440

atccccgagg cccccagaga tggccaggcc tacgtgcgga aggacggcga gtgggtgctg 1500

ctgtctacat ttctg 1515

<210> 49

<211> 1440

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Sc-9-10 DS-Cav 1A 149C Y458C (RSV-F) without signal peptide

<220>

<221> misc_feature

<222> (1360)..(1440)

<223> Foldon Domain

<400> 49

cagaacatca ccgaggaatt ctaccagagc acctgcagcg ccgtgagcaa gggctacctg 60

agcgccctgc ggaccggctg gtacaccagc gtgatcacca tcgagctgtc caacatcaaa 120

gaaaacaagt gcaacggcac cgacgccaaa gtgaagctga tcaagcagga actggacaag 180

tacaagaacg ccgtgaccga gctgcagctg ctgatgcaga gcacccccgc caccggatct 240

ggcagcgcca tttgcagcgg cgtggccgtg tgtaaagtgc tgcacctgga aggcgaagtg 300

aacaagatca agtccgccct gctgtccacc aacaaggccg tggtgtccct gagcaacggc 360

gtgagcgtgc tgaccttcaa ggtgctggat ctgaagaact acatcgacaa gcagctgctg 420

cccatcctga acaagcagag ctgcagcatc agcaacatcg agacagtgat cgagttccag 480

cagaagaaca accggctgct ggaaatcacc cgggagttca gcgtgaacgc cggagtgacc 540

acccccgtgt ccacctacat gctgaccaac agcgagctgc tgtccctgat caatgacatg 600

cccatcacca acgaccagaa aaagctgatg agcaacaacg tgcagatcgt gcggcagcag 660

agctactcca tcatgtgcat catcaaagaa gaggtgctgg cctacgtggt gcagctgccc 720

ctgtacggcg tgatcgacac cccctgctgg aagctgcaca ccagccccct gtgcacaacc 780

aacaccaaag agggcagcaa catctgcctg acccggaccg accggggctg gtactgcgac 840

aacgccggca gcgtgtcctt ctttccacag gccgagacat gcaaggtgca gagcaaccgg 900

gtgttctgcg acaccatgaa cagccggacc ctgccctccg aagtgaacct gtgcaacgtg 960

gacatcttca accccaagta cgactgcaag atcatgacct ccaagaccga cgtgtccagc 1020

tccgtgatca cctccctggg cgccatcgtg tcctgctacg gcaagaccaa gtgcaccgcc 1080

agcaacaaga acagaggcat catcaagacc ttcagcaacg gctgcgacta cgtgtccaat 1140

aagggcgtgg acaccgtgtc cgtgggcaac acactgtact gcgtgaataa gcaggaaggc 1200

aagagcctgt acgtgaaggg cgagcccatc atcaacttct acgaccccct ggtgttcccc 1260

agcgacgagt tcgacgctag catcagccag gtgaacgaga agatcaacca gagcctggcc 1320

ttcatcagaa agagcgacga actgctgtcc gccatcggcg gctacatccc cgaggccccc 1380

agagatggcc aggcctacgt gcggaaggac ggcgagtggg tgctgctgtc tacatttctg 1440

<210> 50

<211> 528

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F-SpyTag-C tag

<220>

<221> MISC_FEATURE

<222> (1)..(25)

<223> Signal peptide

<220>

<221> MISC_FEATURE

<222> (479)..(505)

<223> Foldon Domain

<220>

<221> MISC_FEATURE

<222> (506)..(511)

<223> (GSG)2 linker

<220>

<221> MISC_FEATURE

<222> (512)..(524)

<223> Spytag

<220>

<221> MISC_FEATURE

<222> (525)..(527)

<223> C tag

<400> 50

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr

1 5 10 15

Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe

20 25 30

Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu

35 40 45

Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile

50 55 60

Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys

65 70 75 80

Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu

85 90 95

Met Gln Ser Thr Pro Ala Thr Gly Ser Gly Ser Ala Ile Cys Ser Gly

100 105 110

Val Ala Val Cys Lys Val Leu His Leu Glu Gly Glu Val Asn Lys Ile

115 120 125

Lys Ser Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser Leu Ser Asn

130 135 140

Gly Val Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile

145 150 155 160

Asp Lys Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser

165 170 175

Asn Ile Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu

180 185 190

Glu Ile Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr Thr Pro Val

195 200 205

Ser Thr Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp

210 215 220

Met Pro Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val Gln

225 230 235 240

Ile Val Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu

245 250 255

Val Leu Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr

260 265 270

Pro Cys Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys

275 280 285

Glu Gly Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys

290 295 300

Asp Asn Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys

305 310 315 320

Val Gln Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser Arg Thr Leu

325 330 335

Pro Ser Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr

340 345 350

Asp Cys Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser Ser Val Ile

355 360 365

Thr Ser Leu Gly Ala Ile Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr

370 375 380

Ala Ser Asn Lys Asn Arg Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys

385 390 395 400

Asp Tyr Val Ser Asn Lys Gly Val Asp Thr Val Ser Val Gly Asn Thr

405 410 415

Leu Tyr Cys Val Asn Lys Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly

420 425 430

Glu Pro Ile Ile Asn Phe Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu

435 440 445

Phe Asp Ala Ser Ile Ser Gln Val Asn Glu Lys Ile Asn Gln Ser Leu

450 455 460

Ala Phe Ile Arg Lys Ser Asp Glu Leu Leu Ser Ala Ile Gly Gly Tyr

465 470 475 480

Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly

485 490 495

Glu Trp Val Leu Leu Ser Thr Phe Leu Gly Ser Gly Gly Ser Gly Ala

500 505 510

His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Glu Pro Glu Ala

515 520 525

<210> 51

<211> 1010

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> Sc-9-10 DS-Cav1 A149C Y458C (RSV-F)

<220>

<221> MISC_FEATURE

<222> (1)..(25)

<223> Signal peptide

<220>

<221> MISC_FEATURE

<222> (479)..(505)

<223> Foldon Domain

<400> 51

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr

1 5 10 15

Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe

20 25 30

Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu

35 40 45

Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile

50 55 60

Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys

65 70 75 80

Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu

85 90 95

Met Gln Ser Thr Pro Ala Thr Gly Ser Gly Ser Ala Ile Cys Ser Gly

100 105 110

Val Ala Val Cys Lys Val Leu His Leu Glu Gly Glu Val Asn Lys Ile

115 120 125

Lys Ser Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser Leu Ser Asn

130 135 140

Gly Val Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile

145 150 155 160

Asp Lys Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser

165 170 175

Asn Ile Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu

180 185 190

Glu Ile Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr Thr Pro Val

195 200 205

Ser Thr Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp

210 215 220

Met Pro Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val Gln

225 230 235 240

Ile Val Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu

245 250 255

Val Leu Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr

260 265 270

Pro Cys Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys

275 280 285

Glu Gly Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys

290 295 300

Asp Asn Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys

305 310 315 320

Val Gln Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser Arg Thr Leu

325 330 335

Pro Ser Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr

340 345 350

Asp Cys Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser Ser Val Ile

355 360 365

Thr Ser Leu Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr

370 375 380

Ile Leu Thr Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr

385 390 395 400

Glu Glu Phe Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu

405 410 415

Ser Ala Leu Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu

420 425 430

Ser Asn Ile Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys

435 440 445

Leu Ile Lys Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu

450 455 460

Gln Leu Leu Met Gln Ser Thr Pro Ala Thr Gly Ser Gly Ser Ala Ile

465 470 475 480

Cys Ser Gly Val Ala Val Cys Lys Val Leu His Leu Glu Gly Glu Val

485 490 495

Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser

500 505 510

Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys

515 520 525

Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys

530 535 540

Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn

545 550 555 560

Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr

565 570 575

Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu

580 585 590

Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn

595 600 605

Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile

610 615 620

Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val

625 630 635 640

Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr

645 650 655

Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly

660 665 670

Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu

675 680 685

Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser

690 695 700

Arg Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn

705 710 715 720

Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser

725 730 735

Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys Tyr Gly Lys Thr

740 745 750

Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile Lys Thr Phe Ser

755 760 765

Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp Thr Val Ser Val

770 775 780

Gly Asn Thr Leu Tyr Cys Val Asn Lys Gln Glu Gly Lys Ser Leu Tyr

785 790 795 800

Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro Leu Val Phe Pro

805 810 815

Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn Glu Lys Ile Asn

820 825 830

Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu Leu Ser Ala Ile

835 840 845

Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg

850 855 860

Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Gly Ala Ile Val

865 870 875 880

Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly

885 890 895

Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly

900 905 910

Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Cys Val Asn Lys Gln

915 920 925

Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr

930 935 940

Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln

945 950 955 960

Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp

965 970 975

Glu Leu Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp

980 985 990

Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr

995 1000 1005

Phe Leu

1010

<210> 52

<211> 480

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> sc9-10DS-Cav 1A 149C Y458C (RSV-F) without signal peptide

<220>

<221> MISC_FEATURE

<222> (454)..(480)

<223> Foldon Domain

<400> 52

Gln Asn Ile Thr Glu Glu Phe Tyr Gln Ser Thr Cys Ser Ala Val Ser

1 5 10 15

Lys Gly Tyr Leu Ser Ala Leu Arg Thr Gly Trp Tyr Thr Ser Val Ile

20 25 30

Thr Ile Glu Leu Ser Asn Ile Lys Glu Asn Lys Cys Asn Gly Thr Asp

35 40 45

Ala Lys Val Lys Leu Ile Lys Gln Glu Leu Asp Lys Tyr Lys Asn Ala

50 55 60

Val Thr Glu Leu Gln Leu Leu Met Gln Ser Thr Pro Ala Thr Gly Ser

65 70 75 80

Gly Ser Ala Ile Cys Ser Gly Val Ala Val Cys Lys Val Leu His Leu

85 90 95

Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys

100 105 110

Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val

115 120 125

Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn

130 135 140

Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln

145 150 155 160

Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn

165 170 175

Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu

180 185 190

Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys

195 200 205

Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile

210 215 220

Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro

225 230 235 240

Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro

245 250 255

Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg

260 265 270

Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe

275 280 285

Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp

290 295 300

Thr Met Asn Ser Arg Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val

305 310 315 320

Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr

325 330 335

Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys

340 345 350

Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile

355 360 365

Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp

370 375 380

Thr Val Ser Val Gly Asn Thr Leu Tyr Cys Val Asn Lys Gln Glu Gly

385 390 395 400

Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro

405 410 415

Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn

420 425 430

Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu

435 440 445

Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln

450 455 460

Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu

465 470 475 480

<210> 53

<211> 1704

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F DS-Cav1-SpyTag-C tag

<220>

<221> misc_feature

<222> (1)..(75)

<223> Signal peptide

<220>

<221> misc_feature

<222> (1552)..(1632)

<223> Foldon Domain

<220>

<221> misc_feature

<222> (1633)..(1650)

<223> (GSG)2 linker

<220>

<221> misc_feature

<222> (1651)..(1689)

<223> Spytag

<220>

<221> misc_feature

<222> (1690)..(1701)

<223> C tag

<220>

<221> misc_feature

<222> (1702)..(1704)

<223> stop codon

<400> 53

atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc 60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg 120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag 180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag 240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc 300

cccgccacca acaacagagc cagaagagag ctgccccggt tcatgaacta caccctgaac 360

aacgccaaga aaaccaacgt gaccctgagc aagaagagaa agagaagatt cctgggcttc 420

ctgctgggcg tgggcagcgc cattgccagc ggcgtggccg tgtgtaaagt gctgcacctg 480

gaaggcgaag tgaacaagat caagtccgcc ctgctgtcca ccaacaaggc cgtggtgtcc 540

ctgagcaacg gcgtgagcgt gctgaccttc aaggtgctgg atctgaagaa ctacatcgac 600

aagcagctgc tgcccatcct gaacaagcag agctgcagca tcagcaacat cgagacagtg 660

atcgagttcc agcagaagaa caaccggctg ctggaaatca cccgggagtt cagcgtgaac 720

gccggagtga ccacccccgt gtccacctac atgctgacca acagcgagct gctgtccctg 780

atcaatgaca tgcccatcac caacgaccag aaaaagctga tgagcaacaa cgtgcagatc 840

gtgcggcagc agagctactc catcatgtgc atcatcaaag aagaggtgct ggcctacgtg 900

gtgcagctgc ccctgtacgg cgtgatcgac accccctgct ggaagctgca caccagcccc 960

ctgtgcacaa ccaacaccaa agagggcagc aacatctgcc tgacccggac cgaccggggc 1020

tggtactgcg acaacgccgg cagcgtgtcc ttctttccac aggccgagac atgcaaggtg 1080

cagagcaacc gggtgttctg cgacaccatg aacagcctga ccctgccctc cgaagtgaac 1140

ctgtgcaacg tggacatctt caaccccaag tacgactgca agatcatgac ctccaagacc 1200

gacgtgtcca gctccgtgat cacctccctg ggcgccatcg tgtcctgcta cggcaagacc 1260

aagtgcaccg ccagcaacaa gaacagaggc atcatcaaga ccttcagcaa cggctgcgac 1320

tacgtgtcca ataagggcgt ggacaccgtg tccgtgggca acacactgta ctacgtgaat 1380

aagcaggaag gcaagagcct gtacgtgaag ggcgagccca tcatcaactt ctacgacccc 1440

ctggtgttcc ccagcgacga gttcgacgcc agcatcagcc aggtgaacga gaagatcaac 1500

cagagcctgg ccttcatcag aaagagcgac gaactgctgt ccgccatcgg cggctacatc 1560

cccgaggccc ccagagatgg ccaggcctac gtgcggaagg acggcgagtg ggtgctgctg 1620

tctacatttc tgggaagcgg aggctctggt gcccatatcg tgatggtgga cgcctacaag 1680

cctaccaaag agcccgaggc ctaa 1704

<210> 54

<211> 1632

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F DS-Cav1

<220>

<221> misc_feature

<222> (1)..(75)

<223> Signal peptide

<220>

<221> misc_feature

<222> (1552)..(1632)

<223> Foldon Domain

<400> 54

atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc 60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg 120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag 180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag 240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc 300

cccgccacca acaacagagc cagaagagag ctgccccggt tcatgaacta caccctgaac 360

aacgccaaga aaaccaacgt gaccctgagc aagaagagaa agagaagatt cctgggcttc 420

ctgctgggcg tgggcagcgc cattgccagc ggcgtggccg tgtgtaaagt gctgcacctg 480

gaaggcgaag tgaacaagat caagtccgcc ctgctgtcca ccaacaaggc cgtggtgtcc 540

ctgagcaacg gcgtgagcgt gctgaccttc aaggtgctgg atctgaagaa ctacatcgac 600

aagcagctgc tgcccatcct gaacaagcag agctgcagca tcagcaacat cgagacagtg 660

atcgagttcc agcagaagaa caaccggctg ctggaaatca cccgggagtt cagcgtgaac 720

gccggagtga ccacccccgt gtccacctac atgctgacca acagcgagct gctgtccctg 780

atcaatgaca tgcccatcac caacgaccag aaaaagctga tgagcaacaa cgtgcagatc 840

gtgcggcagc agagctactc catcatgtgc atcatcaaag aagaggtgct ggcctacgtg 900

gtgcagctgc ccctgtacgg cgtgatcgac accccctgct ggaagctgca caccagcccc 960

ctgtgcacaa ccaacaccaa agagggcagc aacatctgcc tgacccggac cgaccggggc 1020

tggtactgcg acaacgccgg cagcgtgtcc ttctttccac aggccgagac atgcaaggtg 1080

cagagcaacc gggtgttctg cgacaccatg aacagcctga ccctgccctc cgaagtgaac 1140

ctgtgcaacg tggacatctt caaccccaag tacgactgca agatcatgac ctccaagacc 1200

gacgtgtcca gctccgtgat cacctccctg ggcgccatcg tgtcctgcta cggcaagacc 1260

aagtgcaccg ccagcaacaa gaacagaggc atcatcaaga ccttcagcaa cggctgcgac 1320

tacgtgtcca ataagggcgt ggacaccgtg tccgtgggca acacactgta ctacgtgaat 1380

aagcaggaag gcaagagcct gtacgtgaag ggcgagccca tcatcaactt ctacgacccc 1440

ctggtgttcc ccagcgacga gttcgacgcc agcatcagcc aggtgaacga gaagatcaac 1500

cagagcctgg ccttcatcag aaagagcgac gaactgctgt ccgccatcgg cggctacatc 1560

cccgaggccc ccagagatgg ccaggcctac gtgcggaagg acggcgagtg ggtgctgctg 1620

tctacatttc tg 1632

<210> 55

<211> 1557

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F DS-Cav1 without Signal peptide

<220>

<221> misc_feature

<222> (1477)..(1557)

<223> Foldon Domain

<400> 55

cagaacatca ccgaggaatt ctaccagagc acctgcagcg ccgtgagcaa gggctacctg 60

agcgccctgc ggaccggctg gtacaccagc gtgatcacca tcgagctgtc caacatcaaa 120

gaaaacaagt gcaacggcac cgacgccaaa gtgaagctga tcaagcagga actggacaag 180

tacaagaacg ccgtgaccga gctgcagctg ctgatgcaga gcacccccgc caccaacaac 240

agagccagaa gagagctgcc ccggttcatg aactacaccc tgaacaacgc caagaaaacc 300

aacgtgaccc tgagcaagaa gagaaagaga agattcctgg gcttcctgct gggcgtgggc 360

agcgccattg ccagcggcgt ggccgtgtgt aaagtgctgc acctggaagg cgaagtgaac 420

aagatcaagt ccgccctgct gtccaccaac aaggccgtgg tgtccctgag caacggcgtg 480

agcgtgctga ccttcaaggt gctggatctg aagaactaca tcgacaagca gctgctgccc 540

atcctgaaca agcagagctg cagcatcagc aacatcgaga cagtgatcga gttccagcag 600

aagaacaacc ggctgctgga aatcacccgg gagttcagcg tgaacgccgg agtgaccacc 660

cccgtgtcca cctacatgct gaccaacagc gagctgctgt ccctgatcaa tgacatgccc 720

atcaccaacg accagaaaaa gctgatgagc aacaacgtgc agatcgtgcg gcagcagagc 780

tactccatca tgtgcatcat caaagaagag gtgctggcct acgtggtgca gctgcccctg 840

tacggcgtga tcgacacccc ctgctggaag ctgcacacca gccccctgtg cacaaccaac 900

accaaagagg gcagcaacat ctgcctgacc cggaccgacc ggggctggta ctgcgacaac 960

gccggcagcg tgtccttctt tccacaggcc gagacatgca aggtgcagag caaccgggtg 1020

ttctgcgaca ccatgaacag cctgaccctg ccctccgaag tgaacctgtg caacgtggac 1080

atcttcaacc ccaagtacga ctgcaagatc atgacctcca agaccgacgt gtccagctcc 1140

gtgatcacct ccctgggcgc catcgtgtcc tgctacggca agaccaagtg caccgccagc 1200

aacaagaaca gaggcatcat caagaccttc agcaacggct gcgactacgt gtccaataag 1260

ggcgtggaca ccgtgtccgt gggcaacaca ctgtactacg tgaataagca ggaaggcaag 1320

agcctgtacg tgaagggcga gcccatcatc aacttctacg accccctggt gttccccagc 1380

gacgagttcg acgccagcat cagccaggtg aacgagaaga tcaaccagag cctggccttc 1440

atcagaaaga gcgacgaact gctgtccgcc atcggcggct acatccccga ggcccccaga 1500

gatggccagg cctacgtgcg gaaggacggc gagtgggtgc tgctgtctac atttctg 1557

<210> 56

<211> 567

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F DS-Cav1-SpyTag-C tag

<220>

<221> MISC_FEATURE

<222> (1)..(25)

<223> Signal peptide

<220>

<221> MISC_FEATURE

<222> (518)..(544)

<223> Foldon Domain

<220>

<221> MISC_FEATURE

<222> (545)..(550)

<223> joint

<220>

<221> MISC_FEATURE

<222> (551)..(563)

<223> Spytag

<220>

<221> MISC_FEATURE

<222> (564)..(567)

<223> C tag

<400> 56

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr

1 5 10 15

Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe

20 25 30

Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu

35 40 45

Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile

50 55 60

Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys

65 70 75 80

Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu

85 90 95

Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro

100 105 110

Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr

115 120 125

Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val

130 135 140

Gly Ser Ala Ile Ala Ser Gly Val Ala Val Cys Lys Val Leu His Leu

145 150 155 160

Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys

165 170 175

Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val

180 185 190

Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn

195 200 205

Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln

210 215 220

Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn

225 230 235 240

Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu

245 250 255

Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys

260 265 270

Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile

275 280 285

Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro

290 295 300

Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro

305 310 315 320

Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg

325 330 335

Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe

340 345 350

Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp

355 360 365

Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val

370 375 380

Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr

385 390 395 400

Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys

405 410 415

Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile

420 425 430

Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp

435 440 445

Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly

450 455 460

Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro

465 470 475 480

Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn

485 490 495

Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu

500 505 510

Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln

515 520 525

Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu

530 535 540

Gly Ser Gly Gly Ser Gly Ala His Ile Val Met Val Asp Ala Tyr Lys

545 550 555 560

Pro Thr Lys Glu Pro Glu Ala

565

<210> 57

<211> 544

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F DS-Cav1

<220>

<221> MISC_FEATURE

<222> (1)..(25)

<223> Signal peptide

<220>

<221> MISC_FEATURE

<222> (518)..(544)

<223> Foldon Domain

<400> 57

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr

1 5 10 15

Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe

20 25 30

Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu

35 40 45

Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile

50 55 60

Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys

65 70 75 80

Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu

85 90 95

Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro

100 105 110

Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr

115 120 125

Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val

130 135 140

Gly Ser Ala Ile Ala Ser Gly Val Ala Val Cys Lys Val Leu His Leu

145 150 155 160

Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys

165 170 175

Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val

180 185 190

Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn

195 200 205

Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln

210 215 220

Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn

225 230 235 240

Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu

245 250 255

Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys

260 265 270

Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile

275 280 285

Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro

290 295 300

Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro

305 310 315 320

Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg

325 330 335

Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe

340 345 350

Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp

355 360 365

Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val

370 375 380

Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr

385 390 395 400

Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys

405 410 415

Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile

420 425 430

Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp

435 440 445

Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly

450 455 460

Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro

465 470 475 480

Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn

485 490 495

Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu

500 505 510

Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln

515 520 525

Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu

530 535 540

<210> 58

<211> 519

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> RSV-F DS-Cav1 without Signal peptide

<220>

<221> MISC_FEATURE

<222> (493)..(519)

<223> Foldon Domain

<400> 58

Gln Asn Ile Thr Glu Glu Phe Tyr Gln Ser Thr Cys Ser Ala Val Ser

1 5 10 15

Lys Gly Tyr Leu Ser Ala Leu Arg Thr Gly Trp Tyr Thr Ser Val Ile

20 25 30

Thr Ile Glu Leu Ser Asn Ile Lys Glu Asn Lys Cys Asn Gly Thr Asp

35 40 45

Ala Lys Val Lys Leu Ile Lys Gln Glu Leu Asp Lys Tyr Lys Asn Ala

50 55 60

Val Thr Glu Leu Gln Leu Leu Met Gln Ser Thr Pro Ala Thr Asn Asn

65 70 75 80

Arg Ala Arg Arg Glu Leu Pro Arg Phe Met Asn Tyr Thr Leu Asn Asn

85 90 95

Ala Lys Lys Thr Asn Val Thr Leu Ser Lys Lys Arg Lys Arg Arg Phe

100 105 110

Leu Gly Phe Leu Leu Gly Val Gly Ser Ala Ile Ala Ser Gly Val Ala

115 120 125

Val Cys Lys Val Leu His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser

130 135 140

Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val

145 150 155 160

Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys

165 170 175

Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile

180 185 190

Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile

195 200 205

Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr

210 215 220

Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro

225 230 235 240

Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val

245 250 255

Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu Val Leu

260 265 270

Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys

275 280 285

Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly

290 295 300

Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn

305 310 315 320

Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys Val Gln

325 330 335

Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser

340 345 350

Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr Asp Cys

355 360 365

Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser

370 375 380

Leu Gly Ala Ile Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser

385 390 395 400

Asn Lys Asn Arg Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr

405 410 415

Val Ser Asn Lys Gly Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr

420 425 430

Tyr Val Asn Lys Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro

435 440 445

Ile Ile Asn Phe Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp

450 455 460

Ala Ser Ile Ser Gln Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe

465 470 475 480

Ile Arg Lys Ser Asp Glu Leu Leu Ser Ala Ile Gly Gly Tyr Ile Pro

485 490 495

Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp

500 505 510

Val Leu Leu Ser Thr Phe Leu

515

<210> 59

<211> 2088

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gH without signal peptide

<220>

<221> misc_feature

<222> (1077)..(1077)

<223> mutation C > A at position 1077

<220>

<221> misc_feature

<222> (1)..(2082)

<223> extracellular domain

<220>

<221> misc_feature

<222> (2083)..(2088)

<223> transmembrane domain (truncated)

<400> 59

cgatatggcg cagaagccgt atccgaaccg ctggacaaag cgtttcacct actgctcaac 60

acctacggga gacccatccg cttcctgcgt gaaaatacca cccagtgtac ctacaacagc 120

agcctccgta acagcacggt cgtcagggaa aacgccatca gtttcaactt tttccaaagc 180

tataatcaat actatgtatt ccatatgcct cgatgtcttt ttgcgggtcc tctggcggag 240

cagtttctga accaggtaga tctgaccgaa accctggaaa gataccaaca gagacttaac 300

acttacgcgc tggtatccaa agacctggcc agctaccgat ctttttcgca gcagctaaag 360

gcacaagaca gcctaggtga acagcccacc actgtgccac cgcccattga cctgtcaata 420

cctcacgttt ggatgccacc gcaaaccact ccacacggct ggacagaatc acataccacc 480

tcaggactac accgaccaca ctttaaccag acctgtatcc tctttgatgg acacgatcta 540

ctattcagca ccgtcacacc ttgtttgcac caaggctttt acctcatcga cgaactacgt 600

tacgttaaaa taacactgac cgaggacttc ttcgtagtta cggtgtccat agacgacgac 660

acacccatgc tgcttatctt cggccatctt ccacgcgtac ttttcaaagc gccctatcaa 720

cgcgacaact ttatactacg acaaactgaa aaacacgagc tcctggtgct agttaagaaa 780

gatcaactga accgtcactc ttatctcaaa gacccggact ttcttgacgc cgcacttgac 840

ttcaactacc tagacctcag cgcactacta cgtaacagct ttcaccgtta cgccgtggat 900

gtactcaaga gcggtcgatg tcagatgctg gaccgccgca cggtagaaat ggccttcgcc 960

tacgcattag cactgttcgc agcagcccga caagaagagg ccggcgccca agtctccgtc 1020

ccacgggccc tagaccgcca ggccgcactc ttacaaatac aagaatttat gatcacatgc 1080

ctctcacaaa caccaccacg caccacgttg ctgctgtatc ccacggccgt ggacctggcc 1140

aaacgagccc tttggacacc gaatcagatc accgacatca ccagcctcgt acgcctggtc 1200

tacatactct ctaaacagaa tcagcaacat ctcatccccc aatgggcact acgacagatc 1260

gccgactttg ccctaaaact acacaaaacg cacctggcct cttttctttc agccttcgca 1320

cgccaagaac tctacctcat gggcagcctc gtccactcca tgctggtaca tacgacggag 1380

agacgcgaaa tcttcatcgt agaaacgggc ctctgttcat tggccgagct atcacacttt 1440

acgcagttgt tagctcatcc acaccacgaa tacctcagcg acctgtacac accctgttcc 1500

agtagcgggc gacgcgatca ctcgctcgaa cgcctcacgc gtctcttccc cgatgccacc 1560

gtccccgcta ccgttcccgc cgccctctcc atcctatcta ccatgcaacc aagcacgctg 1620

gaaaccttcc ccgacctgtt ttgcttgccg ctcggcgaat ccttctccgc gctgaccgtc 1680

tccgaacacg tcagttatat cgtaacaaac cagtacctga tcaaaggtat ctcctaccct 1740

gtctccacca ccgtcgtagg ccagagcctc atcatcaccc agacggacag tcaaactaaa 1800

tgcgaactga cgcgcaacat gcataccaca cacagcatca cagtggcgct caacatttcg 1860

ctagaaaact gcgccttttg ccaaagcgcc ctgctagaat acgacgacac gcaaggcgtc 1920

atcaacatca tgtacatgca cgactcggac gacgtccttt tcgccctgga tccctacaac 1980

gaagtggtgg tctcatctcc gcgaactcac tacctcatgc ttttgaaaaa cggtacggta 2040

ctagaagtaa ctgacgtcgt cgtggacgcc accgacagtc gtctcctc 2088

<210> 60

<211> 696

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<220>

<223> gH without signal peptide

<220>

<221> MISC_FEATURE

<222> (1)..(694)

<223> extracellular domain

<220>

<221> MISC_FEATURE

<222> (694)..(696)

<223> transmembrane domain (truncated)

<400> 60

Arg Tyr Gly Ala Glu Ala Val Ser Glu Pro Leu Asp Lys Ala Phe His

1 5 10 15

Leu Leu Leu Asn Thr Tyr Gly Arg Pro Ile Arg Phe Leu Arg Glu Asn

20 25 30

Thr Thr Gln Cys Thr Tyr Asn Ser Ser Leu Arg Asn Ser Thr Val Val

35 40 45

Arg Glu Asn Ala Ile Ser Phe Asn Phe Phe Gln Ser Tyr Asn Gln Tyr

50 55 60

Tyr Val Phe His Met Pro Arg Cys Leu Phe Ala Gly Pro Leu Ala Glu

65 70 75 80

Gln Phe Leu Asn Gln Val Asp Leu Thr Glu Thr Leu Glu Arg Tyr Gln

85 90 95

Gln Arg Leu Asn Thr Tyr Ala Leu Val Ser Lys Asp Leu Ala Ser Tyr

100 105 110

Arg Ser Phe Ser Gln Gln Leu Lys Ala Gln Asp Ser Leu Gly Glu Gln

115 120 125

Pro Thr Thr Val Pro Pro Pro Ile Asp Leu Ser Ile Pro His Val Trp

130 135 140

Met Pro Pro Gln Thr Thr Pro His Gly Trp Thr Glu Ser His Thr Thr

145 150 155 160

Ser Gly Leu His Arg Pro His Phe Asn Gln Thr Cys Ile Leu Phe Asp

165 170 175

Gly His Asp Leu Leu Phe Ser Thr Val Thr Pro Cys Leu His Gln Gly

180 185 190

Phe Tyr Leu Ile Asp Glu Leu Arg Tyr Val Lys Ile Thr Leu Thr Glu

195 200 205

Asp Phe Phe Val Val Thr Val Ser Ile Asp Asp Asp Thr Pro Met Leu

210 215 220

Leu Ile Phe Gly His Leu Pro Arg Val Leu Phe Lys Ala Pro Tyr Gln

225 230 235 240

Arg Asp Asn Phe Ile Leu Arg Gln Thr Glu Lys His Glu Leu Leu Val

245 250 255

Leu Val Lys Lys Asp Gln Leu Asn Arg His Ser Tyr Leu Lys Asp Pro

260 265 270

Asp Phe Leu Asp Ala Ala Leu Asp Phe Asn Tyr Leu Asp Leu Ser Ala

275 280 285

Leu Leu Arg Asn Ser Phe His Arg Tyr Ala Val Asp Val Leu Lys Ser

290 295 300

Gly Arg Cys Gln Met Leu Asp Arg Arg Thr Val Glu Met Ala Phe Ala

305 310 315 320

Tyr Ala Leu Ala Leu Phe Ala Ala Ala Arg Gln Glu Glu Ala Gly Ala

325 330 335

Gln Val Ser Val Pro Arg Ala Leu Asp Arg Gln Ala Ala Leu Leu Gln

340 345 350

Ile Gln Glu Phe Met Ile Thr Cys Leu Ser Gln Thr Pro Pro Arg Thr

355 360 365

Thr Leu Leu Leu Tyr Pro Thr Ala Val Asp Leu Ala Lys Arg Ala Leu

370 375 380

Trp Thr Pro Asn Gln Ile Thr Asp Ile Thr Ser Leu Val Arg Leu Val

385 390 395 400

Tyr Ile Leu Ser Lys Gln Asn Gln Gln His Leu Ile Pro Gln Trp Ala

405 410 415

Leu Arg Gln Ile Ala Asp Phe Ala Leu Lys Leu His Lys Thr His Leu

420 425 430

Ala Ser Phe Leu Ser Ala Phe Ala Arg Gln Glu Leu Tyr Leu Met Gly

435 440 445

Ser Leu Val His Ser Met Leu Val His Thr Thr Glu Arg Arg Glu Ile

450 455 460

Phe Ile Val Glu Thr Gly Leu Cys Ser Leu Ala Glu Leu Ser His Phe

465 470 475 480

Thr Gln Leu Leu Ala His Pro His His Glu Tyr Leu Ser Asp Leu Tyr

485 490 495

Thr Pro Cys Ser Ser Ser Gly Arg Arg Asp His Ser Leu Glu Arg Leu

500 505 510

Thr Arg Leu Phe Pro Asp Ala Thr Val Pro Ala Thr Val Pro Ala Ala

515 520 525

Leu Ser Ile Leu Ser Thr Met Gln Pro Ser Thr Leu Glu Thr Phe Pro

530 535 540

Asp Leu Phe Cys Leu Pro Leu Gly Glu Ser Phe Ser Ala Leu Thr Val

545 550 555 560

Ser Glu His Val Ser Tyr Ile Val Thr Asn Gln Tyr Leu Ile Lys Gly

565 570 575

Ile Ser Tyr Pro Val Ser Thr Thr Val Val Gly Gln Ser Leu Ile Ile

580 585 590

Thr Gln Thr Asp Ser Gln Thr Lys Cys Glu Leu Thr Arg Asn Met His

595 600 605

Thr Thr His Ser Ile Thr Val Ala Leu Asn Ile Ser Leu Glu Asn Cys

610 615 620

Ala Phe Cys Gln Ser Ala Leu Leu Glu Tyr Asp Asp Thr Gln Gly Val

625 630 635 640

Ile Asn Ile Met Tyr Met His Asp Ser Asp Asp Val Leu Phe Ala Leu

645 650 655

Asp Pro Tyr Asn Glu Val Val Val Ser Ser Pro Arg Thr His Tyr Leu

660 665 670

Met Leu Leu Lys Asn Gly Thr Val Leu Glu Val Thr Asp Val Val Val

675 680 685

Asp Ala Thr Asp Ser Arg Leu Leu

690 695