阅读说明：本技术 一种鸡胸肉酶解成小分子肽的应用方法 (Application method for chicken breast enzymolysis to produce small molecular peptide ) 是由 吕勤 郭荣杰 赖朝敏 陈鸿旭 周兴江 于 2020-12-21 设计创作，主要内容包括：本发明属于禽肉制品精深加工技术领域,公开了一种鸡胸肉酶解成小分子肽的应用方法,该应用方法为将第一重量份的鸡胸肉经酶组合物水解后,与第二重量份的鸡胸肉经高速斩拌与谷氨酰胺转氨酶反应,得到一种高蛋白四轻即食类鸡胸肉产品。本发明采用包含复合蛋白酶、风味蛋白酶和氨基肽酶的酶组合物对鸡胸肉进行酶解液化,一方面对鸡胸肉进行酶解液化,提供了功能性小分子肽的酶解提取方法,另一方面将酶解后的功能性小分子肽与特添乌鸡肽综合应用,制备出一种即食轻食类鸡肉制品,该产品营养均衡,在营养结构上满足现代身体机能需求,富含蛋白质,满足轻脂肪、轻碳水化合物、轻卡路里、轻盐易被人体消化吸收、便于随身携带的开袋即食便捷肉制品。(The invention belongs to the technical field of deep processing of poultry products, and discloses an application method for hydrolyzing chicken breast meat into small molecular peptides by enzyme. The invention adopts an enzyme composition containing compound protease, flavourzyme and aminopeptidase to carry out enzymolysis liquefaction on chicken breast, on one hand, the chicken breast is subjected to enzymolysis liquefaction, a method for extracting functional micromolecule peptide is provided, on the other hand, the enzymolyzed functional micromolecule peptide and specially-added black-bone chicken peptide are comprehensively applied, and the instant light-eating chicken product is prepared.)

1. An application method of chicken breast enzymolysis to obtain small molecular peptides is characterized in that a first weight part of chicken breast is hydrolyzed by an enzyme composition and then is subjected to high-speed chopping and mixing with a second weight part of chicken breast to react with glutamine transaminase to obtain an instant chicken breast product.

2. The application method of claim 1, wherein the method for hydrolyzing the first weight part of chicken breast with complex enzyme specifically comprises the following steps: unfreezing the first weight part of chicken breast, taking out connective tissues, cutting into small pieces, and mincing into chicken paste by a meat mincer;

uniformly stirring the chicken meat paste and water according to the proportion of 1:1, and adding an enzyme composition for enzymolysis and liquefaction;

carrying out enzyme deactivation treatment after the enzymolysis is finished; and

filtering the mixed solution after enzyme deactivation, and refrigerating the enzymolysis supernatant for later use.

3. The application method of claim 2, wherein the enzymatic liquefaction treatment method comprises the following steps:

placing the mixture of the chicken paste and water in a water bath kettle with the water temperature of 55 ℃, adding an enzyme composition for enzymolysis liquefaction treatment after the temperature is raised to 55 ℃, wherein the solid-liquid ratio is 1:1, and carrying out enzymolysis for 3h at 55 ℃.

4. The use according to any one of claims 1 to 3, wherein the enzyme composition comprises a complex protease, a flavourzyme and an aminopeptidase, the enzyme composition comprising from 0.2% to 0.4% by weight of the chicken emulsion when mixed with water.

5. The method of use according to claim 4, wherein the weight ratio of the complex protease, the flavourzyme and the aminopeptidase is: 1:4:1.

6. The use of claim 5, wherein the activity of each enzyme in the enzyme composition is: the composite protease is more than or equal to 30000U/mg, the flavourzyme is more than or equal to 20000U/mg, and the aminopeptidase is more than or equal to 5000U/mg.

7. The method of use of claim 2, wherein the high speed chopping of the second weight portion of chicken breast with glutamine transaminase comprises:

thawing the second weight part of chicken breast, taking out connective tissues, cutting into small pieces, and mincing into chicken paste by a meat mincer;

adding the enzymolysis supernatant and glutamine transaminase into the chicken paste, uniformly mixing by high-speed chopping, stirring and other processes, molding by a grinding tool, slowly cooking and curing at 70 ℃, baking, packaging and sterilizing to prepare the chicken paste.

8. The method of use as claimed in claim 7 wherein said high speed chopping may be preceded by the addition of nutritional supplements including but not limited to vegetables, nuts, grains.

9. The use of any one of claims 1, 2, and 7, wherein the weight ratio of the first part by weight to the second part by weight of chicken breast is from 1:2 to 1: 4.

10. An instant chicken breast product prepared by the method of use of the chicken breast enzyme of any one of claims 1-9 to form small molecule peptides.

Technical Field

The invention relates to the technical field of poultry meat product processing, in particular to an application method of chicken breast enzymolysis to micromolecular peptide.

Background

The chicken is one of main meat food sources of residents in China, is popular among people due to the characteristics of delicious taste, low fat, high protein, rich in various fatty acids and the like, is tender compared with beef, pork and mutton, contains rich nutrient substances and flavor components such as free amino acids, nucleotides, organic acids, peptides and the like, and has delicious taste due to the independent and mutual synergistic action.

The chicken breast meat of the traditional product is in a block shape, the taste is not good after cooking or seasoning, and high protein is not easy to absorb and digest. The enzymatic hydrolysis method has the advantages of mild conditions, high product safety, capability of well retaining the biological activity of an enzymolysis product and the like, and is widely applied to the field of food processing. The hydrolysis efficiency of different enzymes on the same substrate and the functional characteristics and the components of an enzymolysis product are greatly different, and most of chicken subjected to enzymolysis is used for preparing natural seasonings in the prior art, so that a new application method for chicken breast enzymolysis is urgently needed to be found in the field, and the chicken breast enzymolysis can be conveniently and quickly digested by a human body after being eaten, so that energy is provided for the body.

Disclosure of Invention

In order to solve the problems in the background art, the invention aims to provide a preparation and application method for chicken breast enzymolysis to obtain small molecular peptides.

In order to achieve the purpose, the invention adopts the technical scheme that:

an application method of chicken breast enzymolysis to obtain small molecular peptides comprises the steps of hydrolyzing a first weight part of chicken breast by an enzyme composition, and carrying out high-speed chopping and mixing on the first weight part of chicken breast and a second weight part of chicken breast to react with glutamine transaminase to obtain an instant chicken breast product.

Further, the method for hydrolyzing the chicken breast meat in the first weight part by using the compound enzyme specifically comprises the following steps:

unfreezing the first weight part of chicken breast, taking out connective tissues, cutting into small pieces, and mincing into chicken paste by a meat mincer;

uniformly stirring the chicken meat paste and water according to the proportion of 1:1, and adding an enzyme composition for enzymolysis and liquefaction;

carrying out enzyme deactivation treatment after the enzymolysis is finished; and

filtering the mixed solution after enzyme deactivation, and refrigerating the enzymolysis supernatant for later use.

Further, the enzymolysis liquefaction treatment method comprises the following steps:

placing the mixture of the chicken paste and water in a water bath kettle with the water temperature of 55 ℃, adding an enzyme composition for enzymolysis liquefaction treatment after the temperature is raised to 55 ℃, wherein the solid-liquid ratio is 1:1, and carrying out enzymolysis for 3h at 55 ℃.

Further, the enzyme composition comprises a compound protease, a flavourzyme and an aminopeptidase, and the enzyme composition accounts for 0.2-0.4% of the weight of the chicken meat paste mixed with water, and is preferably 0.3%.

Further, the weight ratio of the compound protease to the flavourzyme to the aminopeptidase is as follows: 1:4:1

Further, the activity of each enzyme in the enzyme composition is: the compound protease is more than or equal to 30000U/mg, the flavourzyme is more than or equal to 20000U/mg, and the aminopeptidase is more than or equal to 5000U/mg

Further, the method for high-speed chopping and mixing the chicken breast with the second weight part to react with glutamine transaminase specifically comprises the following steps:

thawing the second weight part of chicken breast, taking out connective tissues, cutting into small pieces, and mincing into chicken paste by a meat mincer;

adding the enzymolysis supernatant and glutamine transaminase into the chicken paste, uniformly mixing by high-speed chopping, stirring and other processes, molding by a grinding tool, slowly cooking and curing at 70 ℃, baking, packaging and sterilizing to prepare the chicken paste.

Furthermore, nutritional excipients including, but not limited to, vegetables, nuts, and grains may also be added before the high-speed chopping.

Further, the weight ratio of the first weight part to the second weight part of the chicken breast is 1:2-1:4, preferably 1: 2.

The invention also discloses an instant chicken breast product prepared by the application method for preparing the micromolecule peptide by any chicken breast enzymolysis.

Compared with the prior art, the invention has the beneficial effects that:

the invention adopts an enzyme composition containing compound protease, flavourzyme and aminopeptidase to carry out enzymolysis liquefaction on chicken breast, and the action is mainly shown in that on one hand, the enzymolysis liquefaction is carried out on the chicken breast, so that an enzymolysis extraction method of functional micromolecular peptide is provided, on the other hand, the enzymolyzed functional micromolecular peptide is applied, so that an instant light-eating chicken product is prepared, the product meets the requirements of modern body functions on the nutrition structure, is easy to digest and absorb by human bodies, is balanced in nutrition, is a high-protein product, meets the requirements of light fat, light carbohydrate, light calorie, light salt and convenient and portable open bags, is suitable for eating as hungry food for people with large workload, frequent overtime, night boiling or brain transition and irregular daily work and rest, and comprises the following steps: white collar in office, people with requirements for body type and figure control, and the like.

Detailed Description

For further understanding of the present invention, the method and effects of the present invention will be described in further detail with reference to specific examples. It should be noted that the present embodiment is only for further illustration of the present invention and should not be construed as limiting the scope of the present invention, and that those skilled in the art can make modifications and adjustments in a non-essential way based on the above disclosure.

The compound protease used in the examples of the present invention was purchased from Novoxil, the flavourzyme was purchased from Novoxil, and the aminopeptidase enzyme was purchased from Ningxia Xiesheng industries, Ltd.

Example 1

An application method of chicken breast enzymolysis to obtain small molecular peptides comprises the steps of hydrolyzing a first weight part of chicken breast by an enzyme composition, and carrying out high-speed chopping and mixing on the first weight part of chicken breast and a second weight part of chicken breast to react with glutamine transaminase to obtain an instant chicken breast product.

Example 2

The method for hydrolyzing chicken breast by complex enzyme specifically comprises the following steps:

unfreezing the first weight part of chicken breast, taking out connective tissues, cutting into small pieces, and mincing into chicken paste by a meat mincer;

uniformly stirring the chicken meat paste and water according to the proportion of 1:1, and adding an enzyme composition for enzymolysis and liquefaction;

carrying out enzyme deactivation treatment after the enzymolysis is finished; and

filtering the mixed solution after enzyme deactivation, and refrigerating the enzymolysis supernatant for later use.

The method for the enzymolysis liquefaction treatment comprises the following steps: placing the mixture of the chicken paste and water in a water bath kettle with the water temperature of 55 ℃, adding an enzyme composition for enzymolysis liquefaction treatment when the temperature is raised to 55 ℃, wherein the solid-liquid ratio is 1:1, and carrying out enzymolysis for 3h at 55 ℃; the enzyme composition comprises a compound protease, a flavourzyme and an aminopeptidase, and the enzyme composition accounts for 0.2-0.4% of the weight of the chicken meat paste mixed with water, and is preferably 0.3%. The weight ratio of the compound protease to the flavourzyme to the aminopeptidase is as follows: 1:4:1 the activity of each enzyme in the enzyme composition is: the activity of each enzyme in the enzyme composition is: the composite protease is more than or equal to 30000U/mg, the flavourzyme is more than or equal to 20000U/mg, and the aminopeptidase is more than or equal to 5000U/mg.

Example 3

The method for reacting with the second weight part of chicken breast meat by high-speed chopping and mixing and glutamine transaminase reaction specifically comprises the following steps:

thawing the second weight part of chicken breast, taking out connective tissues, cutting into small pieces, and mincing into chicken paste by a meat mincer; and

adding the enzymolysis supernatant and glutamine transaminase into the chicken paste, uniformly mixing by high-speed chopping, stirring and other processes, molding by a grinding tool, slowly cooking and curing at 70 ℃, baking, packaging and sterilizing to prepare the chicken paste.

Wherein, nutritional auxiliary materials can be added before the high-speed chopping, and the nutritional auxiliary materials comprise but are not limited to vegetables, nuts and grains; the weight ratio of the first weight part to the second weight part of the chicken breast is 1:2-1:4, preferably 1: 2.

Example 4

An application method of chicken breast enzymolysis to form small molecular peptides comprises the following steps:

thawing chicken breast, removing connective tissue, cutting into small pieces, and mincing with meat mincer;

uniformly stirring 0.5kg of chicken meat paste and water according to the ratio of 1:1, placing the mixture in a water bath kettle with the water temperature of 55 ℃, adding 1.5g of enzyme composition when the temperature rises to 55 ℃, and carrying out enzymolysis liquefaction treatment (the solid-liquid ratio is 1:1, and enzymolysis is carried out for 3 hours at 55 ℃) when the adding amount ratio of compound protease, flavourzyme and aminopeptidase is 1:4: 1;

carrying out enzyme deactivation treatment at 95 ℃ for 10min after the enzymolysis is finished;

filtering the mixed solution after enzyme deactivation, and refrigerating the enzymolysis supernatant for later use;

adding 1kg of minced chicken into the enzymatic hydrolysate, uniformly mixing with glutamine transaminase, broccoli, water chestnut, badam, walnut and the like by high-speed chopping, stirring and other processes, molding by a mold, slowly cooking and curing at 70 ℃, baking, packaging and sterilizing (at 105 ℃ for 25 min).

Example 5

An application method of chicken breast enzymolysis to form small molecular peptides comprises the following steps:

thawing chicken breast, removing connective tissue, cutting into small pieces, and mincing with meat mincer;

uniformly stirring 0.5kg of chicken meat paste and water according to the ratio of 1:1, placing the mixture in a water bath kettle with the water temperature of 55 ℃, adding 1.0g of enzyme composition when the temperature rises to 55 ℃, and carrying out enzymolysis liquefaction treatment (the solid-liquid ratio is 1:1, and enzymolysis is carried out for 3 hours at 55 ℃) when the adding amount ratio of compound protease, flavourzyme and aminopeptidase is 1:4: 1;

carrying out enzyme deactivation treatment at 95 ℃ for 10min after the enzymolysis is finished;

filtering the mixed solution after enzyme deactivation, and refrigerating the enzymolysis supernatant for later use;

adding 1.5kg of chicken meat paste into the enzymatic hydrolysate, uniformly mixing with glutamine transaminase, carrot, oat, brown rice, walnut and the like by high-speed chopping, stirring and other processes, molding by a mold, slowly cooking and curing at 70 ℃, baking, packaging and sterilizing (105 ℃ and 25 min).

Example 6

An application method of chicken breast enzymolysis to form small molecular peptides comprises the following steps:

thawing chicken breast, removing connective tissue, cutting into small pieces, and mincing with meat mincer;

uniformly stirring 0.5kg of chicken meat paste and water according to the ratio of 1:1, placing the mixture in a water bath kettle with the water temperature of 55 ℃, adding 2.0g of enzyme composition when the temperature rises to 55 ℃, and performing enzymolysis liquefaction treatment (the solid-liquid ratio is 1:1, and enzymolysis is performed for 3 hours at 55 ℃) when the adding amount ratio of compound protease, flavourzyme and aminopeptidase is 1:4: 1;

carrying out enzyme deactivation treatment at 95 ℃ for 10min after the enzymolysis is finished;

filtering the mixed solution after enzyme deactivation, and refrigerating the enzymolysis supernatant for later use;

adding the enzymatic hydrolysate into 2kg of minced chicken meat, uniformly mixing with glutamine transaminase, broccoli, badam, coix seed and the like by high-speed chopping, stirring and other processes, molding by a mold, slowly cooking and curing at 70 ℃, baking, packaging and sterilizing (105 ℃ and 25 min).

Comparative example 1

Compared with the embodiment 4, the addition of the compound protease is eliminated, and the rest components and the process are the same as the embodiment 4.

Comparative example 2

Compared with the example 4, the addition of the flavourzyme is eliminated, and the rest components and the process are the same as the example 4.

Comparative example 3

Compared with the example 4, the addition of aminopeptidase is eliminated, and the rest components and the process are the same as the example 4.

Comparative example 4

Compared with the embodiment 4, the enzymolysis liquefaction step of the enzyme composition is eliminated, and the rest components and the process are the same as the embodiment 4.

Test examples

The instant chicken breast products prepared in the above example 4 and comparative examples 1 to 4 were subjected to in vitro simulated gastrointestinal digestion test to determine the digestibility of each sample, specifically as follows:

respectively taking 10g of samples to be crushed, accurately weighing 1.0g of crushed samples, and adding 4mL of PBS (10 mmol/L NaH)₂PO₄pH 7.0) was homogenized in an ice bath, 9600r/min was repeated twice for 30s, and 13400r/min was repeated twice for 30s, each time at 30s intervals. The pH was adjusted to 2.0. + -. 0.1 with 1M HCl 1ml pepsin solution (0.48: 15ml 0.1M HCl) was added per sample and the homogenate was simulated for digestion of gastric juice in vitro on a 37 ℃ constant temperature shaker for 2h at 160 r/min.

② after the digestion reaction of the gastric juice, rapidly adjusting the pH value of the enzymolysis liquid in the step (i) to about 7.0 by using 1M NaOH solution to terminate the enzymolysis reaction, finally adjusting the pH value to 7.5 +/-0.1, then adding 1.0mL of trypsin solution (0.288 g: 12mL of 0.01M pH value 7.0 PBS) into the reaction system, and simulating the in vitro intestinal juice on a constant temperature oscillator at 37 ℃ for 2h at the rotating speed of 160 r/min. Heating in 100 deg.C boiling water bath for 5min to terminate enzymolysis reaction.

Weighing 3g of each sample subjected to enzymolysis in the second step, and adding pepsin and trypsin to perform two-step stepwise digestion, wherein the specific digestion process is the same as that in the second step. The pepsin hydrolysate and the trypsin hydrolysate were added to 3mL of absolute ethanol, and the mixture was allowed to stand at 4 ℃ for 12 hours and centrifuged (10000 g, 20min, 4 ℃). The enzymatic hydrolysate after alcohol precipitation is centrifuged (4000 g, 15min, 4 ℃) and the supernatant is discarded. The precipitate was dried at 50 ℃ to constant weight, the dried sample data was recorded, and the protein content of the sample before digestion and the dried residue was determined by kjeldahl method. The formula for calculating the digestibility is as follows:

wherein DT is the in vitro digestibility of the protein, W1 is the content (g) of the protein in the dried precipitate after digestion, and W0 is the content (g) of the protein in the sample before digestion.

The test results are shown in table 1:

TABLE 1

。

As can be seen from Table 1, the protein digestibility of example 4 reached 84.19% after gastrointestinal digestion, and the protein digestibility of comparative examples 1-3 was lower than that of example 4 after gastrointestinal digestion but the comparative examples 1-3 differed from each other by a small amount, in which the addition of any one of the enzymes in the enzyme composition was omitted; comparative example 4 is a sample in which enzymatic digestion was abolished, and the rate of protein digestion after gastrointestinal digestion was only 49.61%, which is significantly lower than the rates of digestion of the samples of example 4 and comparative examples 1-3. Therefore, it can be seen from the above results that the digestibility of the instant chicken breast product prepared by carrying out enzymolysis on 3 enzyme compositions in the embodiment of the present invention can be significantly improved, the digestibility of the product without enzymolysis is significantly reduced after removing any one of the enzymes, and the digestibility of the product without enzymolysis is the lowest.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.