阅读说明：本技术 一种提高莫能菌素a组分含量的工艺 (Process for improving content of monensin A component ) 是由 李承立 颜晓冬 靳连标 兰玉龙 钟建祥 于 2020-10-30 设计创作，主要内容包括：本发明公开了一种提高莫能菌素A组分含量的工艺,包括如下步骤：S1、按照3～5:1：190～200的重量比取豆粕粉、玉米浆和水,置于混合容器中混合,得到混合溶液；S2、将混合溶液进行高温灭菌,灭菌后降温处理,得到灭菌混合溶液；S3、向莫能菌素发酵液中分阶段以不同流速加入灭菌混合溶液,进行发酵。本发明的申请人偶然发现发酵中后期(200h以后)菌丝体代谢逐渐开始下降,菌丝体浓度增长逐渐变缓,菌丝体对有机氮源的利用能力也会呈现下降的趋势,而调整氮源投入量和速度可以控制菌丝体代谢,申请人通过调整不同时间段的投放氮源的速率,使得菌丝体的营养平衡,进而使得菌丝体可以长时间保持活力,获得比较好的代谢,迅速提高菌丝体浓度,提高A组分的含量。(The invention discloses a process for improving the content of a component A of monensin, which comprises the following steps: s1, mixing the components in a ratio of 3-5: 1: taking soybean meal powder, corn steep liquor and water according to the weight ratio of 190-200, and mixing in a mixing container to obtain a mixed solution; s2, sterilizing the mixed solution at high temperature, and cooling after sterilization to obtain a sterilized mixed solution; and S3, adding the sterilized mixed solution into the monensin fermentation liquor in stages at different flow rates, and fermenting. The applicant of the invention accidentally finds that the metabolism of the mycelium gradually begins to decline in the middle and later stages of fermentation (after 200 hours), the concentration of the mycelium gradually increases, the utilization capacity of the mycelium to an organic nitrogen source also shows a descending trend, the metabolism of the mycelium can be controlled by adjusting the input amount and speed of the nitrogen source, the applicant enables the nutrition of the mycelium to be balanced by adjusting the speed of putting the nitrogen source in different time periods, so that the mycelium can keep the activity for a long time, better metabolism is obtained, the concentration of the mycelium is rapidly improved, and the content of the component A is improved.)

1. A process for improving the content of a component A of monensin is characterized by comprising the following steps:

s1, taking the soybean meal powder, the corn steep liquor and water according to the weight ratio of 3-5: 1: 190-200, and mixing in a mixing container to obtain a mixed solution;

s2, sterilizing the mixed solution at high temperature, and cooling after sterilization to obtain a sterilized mixed solution;

and S3, adding the sterilized mixed solution into the monensin fermentation liquor in stages at different flow rates, and fermenting.

2. The process for increasing the content of the monensin A component according to claim 1, wherein the step S2 is to sterilize at 121 ℃ for 30min and cool to 30-40 ℃ after sterilization.

3. The process for improving the content of the component A of monensin according to claim 1, wherein in the step S3, when the fermentation broth of monensin is fermented for 200-280 hours, the sterile mixed solution is supplemented at a rate of 13-18L/h; supplementing a sterilized mixed solution at the rate of 9-13L/h when the fermentation of the monensin fermentation liquor is carried out for 280-320 h; and supplementing the sterilized mixed solution at the rate of 7-9L/h when the monensin fermentation liquor is fermented for 320 h.

4. The process for improving the content of the component A of monensin according to claim 3, wherein in the step S3, when the fermentation broth of monensin is fermented for 200-280 hours, the sterile mixed solution is supplemented at a rate of 15L/h; supplementing a sterilized mixed solution at a rate of 10L/h when the monensin fermentation liquor is fermented for 280-320 h; when the fermentation liquor of monensin is fermented for 320h, the sterilized mixed solution is supplemented according to the speed of 8L/h.

Technical Field

The invention relates to the field of veterinary drugs, in particular to a process for improving the content of a component A of monensin.

Background

Monensin (monensin), a polyether antibiotic, was first isolated in 1967 by Haney et al from the fermentation broth of Streptomyces cinnamomea (Streptomyces cinnamunensis). In the 70 s of the 20 th century, the drug began to be marketed as an anticoccidial drug. The feed additive is officially approved in 1974 and 1977 in the United states and Japan, respectively, is acquired in 1985 in China and is applied to production. Currently, more than 40 countries have been used as weighting agents for beef cattle and mutton sheep and growth promoters for pigs. Monensin has no effect on negative bacteria, but has certain inhibiting effect on staphylococcus, bacillus, clostridium, streptococcus and mould (penicillium and candida). Monensin can affect energy metabolism in rumen of ruminant, improve rumen fermentation, increase output ratio of propionic acid and acetic acid, reduce volatile fatty acid, reduce methane generation, and improve protein utilization efficiency. Therefore, the feed utilization rate of the ruminant can be obviously improved, and the growth of the ruminant can be promoted.

The conventional preparation of monensin usually uses an inorganic nitrogen source as a nitrogen source, and controls the metabolism of mycelia by, for example, one-time feeding of ammonium sulfate and feeding of ammonia water in a stream.

The monensin comprises 4 components including components A, B, C and D, wherein the component A is an active ingredient of the monensin product, and the higher the component A, the better the quality of the component A. The nitrogen source quantity required by monensin in different stages in the fermentation process is different, but the existing research only adjusts the preparation method of monensin, so that a large amount of monensin is obtained, but the content of the monensin A component is not improved.

Disclosure of Invention

In order to overcome the problems in the prior art, the invention provides a process for improving the content of a component A of monensin.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a process for increasing the content of a component A of monensin comprises the following steps:

s1, taking the soybean meal powder, the corn steep liquor and water according to the weight ratio of 3-5: 1: 190-200, and mixing in a mixing container to obtain a mixed solution;

s2, sterilizing the mixed solution at high temperature, and cooling after sterilization to obtain a sterilized mixed solution;

and S3, adding the sterilized mixed solution into the monensin fermentation liquor in stages at different flow rates, and fermenting.

Further, the step S2 is to sterilize at 121 ℃ for 30min, and cool to 30-40 ℃ after sterilization.

Further, in the step S3, when the monensin fermentation liquor is fermented for 200-280 hours, a sterilization mixed solution is supplemented according to the speed of 13-18L/h; supplementing a sterilized mixed solution at the rate of 9-13L/h when the fermentation of the monensin fermentation liquor is carried out for 280-320 h; and supplementing the sterilized mixed solution at the rate of 7-9L/h when the monensin fermentation liquor is fermented for 320 h.

Specifically, in the step S3, when the monensin fermentation liquor is fermented for 200-280 hours, the sterilization mixed solution is supplemented according to the rate of 15L/h; supplementing a sterilized mixed solution at a rate of 10L/h when the monensin fermentation liquor is fermented for 280-320 h; when the fermentation liquor of monensin is fermented for 320h, the sterilized mixed solution is supplemented according to the speed of 8L/h.

Compared with the prior art, the invention has the following beneficial effects:

(1) the applicant of the invention accidentally finds that the metabolism of the mycelium gradually begins to decline in the middle and later stages of fermentation (after 200 h), the concentration of the mycelium gradually increases, the utilization capacity of the mycelium to the organic nitrogen source also shows a descending trend, and the metabolism of the mycelium can be controlled by adjusting the input amount and speed of the nitrogen source, so that the content of the component A is adjusted; the applicant finds that by adjusting the nitrogen source feeding speed in different time periods, the inhibition of excess nutrient substances in the culture medium on mycelium metabolism can be prevented, so that the mycelium nutrition is balanced, the mycelium development is accelerated, the mycelium concentration is rapidly improved, the mycelium can keep vitality for a long time, better metabolism is obtained, the titer is improved, the component A is the main active component of a product metabolized by the mycelium, the metabolism can be continuously carried out under the high vitality state of the mycelium, the component A of the metabolite can be effectively improved, and the product quality is ensured.

(2) Compared with the inorganic nitrogen source, the organic nitrogen source can slowly release the nitrogen source, so that the nitrogen source can be used by the mycelium for a long time, the defect that the mycelium is lack of the nitrogen source in the later stage is avoided, a small amount of saccharides and growth factors can be provided besides the nitrogen source for the metabolism of the mycelium, the purposes of vigorous metabolism of the mycelium and quick increase of the concentration of the mycelium can be achieved by using the organic nitrogen source, and the soybean meal and the corn steep liquor as the nitrogen source are low in price and can reduce the preparation cost.

(3) The organic nitrogen source (soybean meal and corn steep liquor) adopted by the invention has a slower utilization speed, so that sufficient nitrogen source can be ensured to be used in the middle and later stages of mycelium metabolism, the generation of metabolite is ensured, the component A is improved, and the corn steep liquor contains a small amount of trace elements, so that the metabolism of the monensin product mycelium can be promoted.

Detailed Description

The present invention is further illustrated by the following examples, which include, but are not limited to, the following examples.

Example 1

40kg of soybean meal powder and 10kg of corn steep liquor are taken and added into 1950L of water to prepare 2000L of mixed solution; sterilizing the mixed solution at 121 deg.C for 30min, naturally cooling to 35 deg.C, fermenting for 200 hr to obtain fermented solution of 44m³Adding the sterilized mixed solution into the monensin fermentation liquor at a flow rate of 15L/h; when the fermentation time reaches 280h, the sterilized mixed solution is fed in at the speed of 10L/h; when the fermentation time reaches 320h, the sterilized mixed solution is fed in at a rate of 8L/h until the mixed solution is placed in a tank.

Comparative example 1

Is not at 44m³Adding the sterilized mixed solution into the monensin fermentation liquid, and performing normal fermentation treatment under the same conditions as in example 1 until the fermentation liquid is fermentedAnd (5) putting the pot.

Example 2

40kg of soybean meal powder and 10kg of corn steep liquor are taken and added into 1950L of water to prepare 2000L of mixed solution; sterilizing the mixed solution at 121 deg.C for 30min, naturally cooling to 35 deg.C, fermenting for 200 hr to obtain fermented solution of 44m³The mixed solution after being sterilized is added into the monensin fermentation liquor at the speed of 15L/h until the fermentation liquor is put into a tank.

Example 3

30kg of soybean meal powder and 10kg of corn steep liquor are taken and added into 1970L of water to prepare 2000L of mixed solution; sterilizing the mixed solution at 121 deg.C for 30min, naturally cooling to 35 deg.C, fermenting for 200 hr to obtain fermented solution of 44m³Adding the sterilized mixed solution into the monensin fermentation liquor at a flow rate of 18L/h; when the fermentation time reaches 280h, the sterilized mixed solution is fed in at the speed of 13L/h; when the fermentation time reaches 320h, the sterilized mixed solution is fed in at a rate of 9L/h until the mixed solution is placed in a tank.

Example 4

Adding 50kg of soybean meal powder and 10kg of corn steep liquor into 1940L of water to prepare 2000L of mixed solution; sterilizing the mixed solution at 121 deg.C for 30min, naturally cooling to 35 deg.C, fermenting for 200 hr to obtain fermented solution of 44m³Adding the sterilized mixed solution into the monensin fermentation liquor at the speed of 13L/h; when the fermentation time reaches 280h, feeding the sterilized mixed solution at the rate of 9L/h; when the fermentation time reaches 320h, the sterilized mixed solution is fed in at a rate of 7L/h until the mixed solution is placed in a tank.

The fermented mycelia of examples 1 to 4 and comparative example 1 were tested, and the results are shown in Table 1.

TABLE 1

As can be seen from Table 1, the biomass, titer, and growth of the A fraction were the largest during the culture, hyphal metabolism was the most vigorous, and the A fraction was the most elevated during the culture.

Comparing biomass, after fermenting for 200h, adding organic nitrogen source into monensin fermentation liquor, detecting when the fermentation liquor is placed in a tank, wherein the biomass of mycelia added with the organic nitrogen source is obviously increased compared with the biomass of mycelia without the organic nitrogen source, and the organic nitrogen source is added into the monensin fermentation liquor at different speeds according to different time periods, the biomass obtained is obviously higher than the biomass of the organic nitrogen source which is always added at a fixed speed, so that the metabolism of the mycelia is in a rapid stage when the fermentation is just performed, more nutrients are utilized, and the metabolism of the mycelia in the stage can not be ensured by the nutrients in the basic culture medium, therefore, the organic nitrogen source is added at the stage, the metabolic capacity of the mycelia is gradually weakened along with the side length of the fermentation culture time, the required nutrients are gradually reduced, and the rate of adding the organic nitrogen source is gradually reduced, the supplement at different rates is carried out in different time periods, so that the requirement of mycelium growth can be met practically, the growth activity of the mycelium is kept, better metabolism is obtained, and the concentration of the mycelium is improved rapidly.

Comparing the contents of the components A of the three groups, the content of the component A detected by the tank-placing in the embodiment 1 is far higher than that of the component A in the embodiment 2 and the comparative example, and the method can prove that the nutrition of the hyphae is balanced by adjusting the nitrogen source feeding rates in different time periods, so that the hyphae can keep vitality for a long time, obtain better metabolism, rapidly improve the concentration of the hyphae and improve the content of the component A.

Comparing the biological values of the three groups, it is obvious that the biological value of the strain when the strain is put into the tank in example 1 is higher than that of example 2 and the comparative example, and it can be proved that the content of the component A can be improved by adjusting the nitrogen source adding speed in different time periods, so that the strain put into the tank has higher bioavailability.

The above-mentioned embodiment is only one of the preferred embodiments of the present invention, and should not be used to limit the scope of the present invention, but all the insubstantial modifications or changes made within the spirit and scope of the main design of the present invention, which still solve the technical problems consistent with the present invention, should be included in the scope of the present invention.