阅读说明：本技术 一种龋齿致病产酸菌增菌初筛培养基及其制备方法和使用方法 (Primary screening culture medium for increasing bacteria of caries pathogenic acid-producing bacteria and preparation method and using method thereof ) 是由 王国柱 于 2020-06-20 设计创作，主要内容包括：本发明涉及一种龋齿致病产酸菌增菌初筛培养基及其制备方法和使用方法,属于微生物培养领域。本发明提供了一种龋齿致病产酸菌增菌初筛培养基,包括以下重量份的组分：淀粉10～20份、磷酸二氢钾0.1～0.3份、糖类4～6份、氯化钠0.4～0.6份、水80～120份、指示剂0.003～0.007份。本申请的培养基配方简单,使用方便,以淀粉为原料,适宜工业化生产,能够满足龋齿致病产酸菌的生长过程中的营养需要,同时大大缩短龋齿致病产酸菌的培养时间。用于牙齿和口腔部位细菌的培养时,通过细菌分解培养基内糖分产酸能力不同,使pH值呈现不同梯度的变化,颜色变化肉眼可见,pH值越低则患龋齿的风险越高。(The invention relates to a primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries, a preparation method and a using method thereof, belonging to the field of microbial culture. The invention provides a primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries, which comprises the following components in parts by weight: 10-20 parts of starch, 0.1-0.3 part of monopotassium phosphate, 4-6 parts of saccharides, 0.4-0.6 part of sodium chloride, 80-120 parts of water and 0.003-0.007 part of indicator. The culture medium has a simple formula, is convenient to use, takes starch as a raw material, is suitable for industrial production, can meet the nutritional requirements in the growth process of the dental caries pathogenic acid-producing bacteria, and greatly shortens the culture time of the dental caries pathogenic acid-producing bacteria. When the microbial agent is used for culturing bacteria in teeth and oral cavity parts, the pH value presents different gradient changes through different sugar acid production capacities in the bacteria decomposition culture medium, the color change can be seen by naked eyes, and the lower the pH value, the higher the risk of dental caries.)

1. A primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries is characterized by comprising the following components in parts by weight: 10-20 parts of starch, 0.1-0.3 part of monopotassium phosphate, 4-6 parts of saccharides, 0.4-0.6 part of sodium chloride, 80-120 parts of water and 0.003-0.007 part of indicator.

2. The primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries according to claim 1, wherein said saccharide is glucose and/or sucrose.

3. The primary screening medium for increasing the number of bacteria of an acid-producing bacterium causing dental caries according to claim 1, wherein the indicator is bromocresol green.

4. The primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries according to claim 3, wherein the indicator is 0.005 part of bromocresol green.

5. The primary screening culture medium for increasing the number of bacteria of the dental caries pathogenic acid-producing bacteria according to any one of claims 1 to 3, which is characterized by comprising the following components in parts by weight: 20 parts of starch, 0.3 part of monopotassium phosphate, 4 parts of glucose, 0.4 part of sodium chloride, 80 parts of water and 0.003 part of bromocresol green.

6. The primary screening culture medium for increasing the number of bacteria of the dental caries pathogenic acid-producing bacteria according to any one of claims 1 to 3, which is characterized by comprising the following components in parts by weight: 10 parts of starch, 0.2 part of monopotassium phosphate, 6 parts of cane sugar, 0.6 part of sodium chloride, 120 parts of water and 0.005 part of bromocresol green.

7. The primary screening culture medium for increasing the number of bacteria of the dental caries pathogenic acid-producing bacteria according to any one of claims 1 to 3, which is characterized by comprising the following components in parts by weight: 15 parts of starch, 0.1 part of monopotassium phosphate, 5 parts of glucose, 0.5 part of sodium chloride, 100 parts of water and 0.007 part of bromocresol green.

8. The preparation method of the primary screening culture medium for increasing the number of bacteria of the caries pathogenic acid-producing bacteria according to any one of claims 1 to 7, characterized by comprising the following steps:

(1) putting starch and water into a heat-resistant container, and marking the water level height;

(2) heating and boiling for 20-30 min;

(3) supplementing water to the original water level height;

(4) adding sugar, potassium dihydrogen phosphate, sodium chloride and indicator to obtain the primary screening culture medium for increasing the number of bacteria of the dental caries pathogenic acid-producing bacteria.

9. The use method of the primary screening culture medium for increasing the number of bacteria of the caries pathogenic acid-producing bacteria as claimed in any one of claims 1 to 7, characterized by comprising the following steps:

(1) inoculating the sample into a culture medium after sampling the teeth;

(2) culturing the inoculated culture medium for 18-24 h at the temperature of 36-38 ℃;

(3) and (4) measuring the pH value of the culture medium and judging the infection risk of the cariogenic tooth.

Technical Field

The invention relates to the field of microbial culture, in particular to a primary screening culture medium for increasing bacteria of caries pathogenic acid-producing bacteria, a preparation method and a use method thereof.

Background

The dental caries has high morbidity and wide distribution, is a main common disease of the oral cavity and one of the most common diseases of human beings, can cause secondary pulpitis and periapical periodontitis and even can cause alveolar bone and jaw inflammation. If not treated in time, the lesion continues to develop, forming a cavity, eventually the crown is completely destroyed and disappears, the end result of which is the loss of teeth.

Bacteria are a prerequisite for caries, and the most common of the numerous bacteria that induce caries is streptococcus mutans. Streptococcus mutans living in human body can rapidly ferment various carbohydrates to generate a large amount of acid, can reduce local pH to below 5.5, and can continue to live and produce acid at pH 4.5 due to strong acid resistance of Streptococcus mutans. The local pH drop is maintained for a considerable time, avoiding the buffering action of saliva, thus causing local hard tissue to drop, inducing the onset of caries lesions. Culturing and detecting the quantity of the caries-causing acid-producing bacteria is an important method for judging the susceptibility value of the caries-causing acid-producing bacteria.

Disclosure of Invention

The invention aims to provide a primary screening culture medium for increasing the number of bacteria of caries-causing acid-producing bacteria, which can meet all requirements for the growth of the caries-causing acid-producing bacteria and can greatly shorten the culture time.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries, which comprises the following components in parts by weight: 10-20 parts of starch, 0.1-0.3 part of monopotassium phosphate, 4-6 parts of saccharides, 0.4-0.6 part of sodium chloride, 80-120 parts of water and 0.003-0.007 part of indicator.

Preferably, the saccharide is glucose and/or sucrose.

Preferably, the indicator is bromocresol green.

Preferably, the indicator is 0.005 part of bromocresol green.

Preferably, the culture medium comprises the following components in parts by weight: 10 parts of starch, 0.1 part of monopotassium phosphate, 4 parts of glucose, 0.4 part of sodium chloride, 80 parts of water and 0.003 part of bromocresol green.

Preferably, the culture medium comprises the following components in parts by weight: 20 parts of starch, 0.2 part of monopotassium phosphate, 6 parts of cane sugar, 0.6 part of sodium chloride, 120 parts of water and 0.005 part of bromocresol green.

Preferably, the culture medium comprises the following components in parts by weight: 15 parts of starch, 0.3 part of monopotassium phosphate, 5 parts of glucose, 0.5 part of sodium chloride, 100 parts of water and 0.004 part of bromocresol green.

The invention also provides a preparation method of the primary screening culture medium for increasing the number of bacteria of the acid-producing bacteria causing dental caries, which comprises the following steps:

(1) putting starch and water into a heat-resistant container, and marking the water level height;

(2) heating and boiling for 20-30 min;

(3) supplementing water to the original water level height;

(4) adding sugar, potassium dihydrogen phosphate, sodium chloride and indicator to obtain the primary screening culture medium for increasing the number of bacteria of the dental caries pathogenic acid-producing bacteria.

The invention also provides a use method of the primary screening culture medium for increasing the number of bacteria of the caries pathogenic acid-producing bacteria, which comprises the following steps:

(1) inoculating the sample into a culture medium after sampling the teeth;

(2) culturing the inoculated culture medium for 18-24 h at the temperature of 36-38 ℃;

(3) and (4) measuring the pH value of the culture medium and judging the infection risk of the cariogenic tooth.

The invention provides a primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries, which comprises the following components in parts by weight: 10-20 parts of starch, 0.1-0.3 part of monopotassium phosphate, 4-6 parts of saccharides, 0.4-0.6 part of sodium chloride, 80-120 parts of water and 0.003-0.007 part of indicator. The culture medium has a simple formula, is convenient to use, takes starch as a raw material, is suitable for industrial production, can meet the nutritional requirements in the growth process of the dental caries pathogenic acid-producing bacteria, and greatly shortens the culture time of the dental caries pathogenic acid-producing bacteria. When the microbial agent is used for culturing bacteria in teeth and oral cavity parts, the pH value presents different gradient changes through different sugar acid production capacities in the bacteria decomposition culture medium, the color change can be seen by naked eyes, and the lower the pH value, the higher the risk of dental caries.

Drawings

FIG. 1 is the result of the culture medium of the present invention after culturing caries-causing acid-producing bacteria for 24 h;

FIG. 2 shows the result of culturing caries-causing acid-producing bacteria in DTA culture medium for 48 hr.

Detailed Description

The invention provides a primary screening culture medium for increasing the number of bacteria of acid-producing bacteria causing dental caries, which comprises the following components in parts by weight: 10-20 parts of starch, 0.1-0.3 part of monopotassium phosphate, 4-6 parts of saccharides, 0.4-0.6 part of sodium chloride, 80-120 parts of water and 0.003-0.007 part of indicator.

In the present invention, the weight part of the starch is preferably 12 to 18 parts, more preferably 14 to 16 parts, and still more preferably 15 parts.

In the present invention, the weight part of the monopotassium phosphate is preferably 0.15 to 0.25 part, and more preferably 0.2 part.

In the present invention, the amount of the saccharide is preferably 4.5 to 5.5 parts by weight, and more preferably 5 parts by weight.

In the present invention, the sodium chloride is preferably 0.45 to 0.55 part by weight, and more preferably 0.5 part by weight.

In the present invention, the water is preferably 90 to 110 parts by weight, more preferably 95 to 105 parts by weight, and still more preferably 100 parts by weight.

In the present invention, the indicator is preferably 0.004 to 0.006 part by weight, and more preferably 0.005 part by weight.

In the present invention, the saccharide is preferably glucose and/or sucrose.

In the present invention, the indicator is preferably bromocresol green.

The invention also provides a preparation method of the primary screening culture medium for increasing the number of bacteria of the acid-producing bacteria causing dental caries, which preferably comprises the following steps:

(1) putting starch and water into a heat-resistant container, and marking the water level height;

(2) heating and boiling for 20-30 min;

(3) supplementing water to the original water level height;

(4) adding sugar, potassium dihydrogen phosphate, sodium chloride and indicator to obtain blue suspension, and obtaining primary screening culture medium for increasing bacteria of the dental caries pathogenic acid-producing bacteria;

(5) filling 3ml of culture medium in each detection tube;

(6) the test tubes were sterilized at 121 ℃ for 15 min.

The invention also provides a use method of the primary screening culture medium for increasing the number of bacteria of the acid-producing bacteria causing dental caries, which preferably comprises the following steps:

(1) the samples were inoculated into the culture medium after 4 samples were rubbed on the teeth;

(2) culturing the inoculated culture medium at 37 ℃ for 24 h;

(3) and (4) measuring the pH value of the culture medium and judging the infection risk of the cariogenic tooth.

The primary screening medium for increasing the number of bacteria of the caries-causing acid-producing bacteria provided by the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.