阅读说明：本技术 一种盐酸小檗碱与左氧氟沙星高效复合抑菌剂 (High-efficiency composite bacteriostatic agent containing berberine hydrochloride and levofloxacin ) 是由 郭钦 彭凯 张春艳 蔡佳惠 于 2020-03-24 设计创作，主要内容包括：本发明公开了一种盐酸小檗碱与左氧氟沙星高效复合抑菌剂,属于医药领域。一种盐酸小檗碱与左氧氟沙星高效复合抑菌剂,其特征在于在抑制耐甲氧西林金黄色葡萄球菌时,以重量百分比计含有以下成分：盐酸小檗碱7.2～23.8％；左氧氟沙星0.2～3.1％；余量为无菌水。高效复合抑菌剂对耐甲氧西林金黄色葡萄球菌(MRSA)及普通金黄色葡萄球菌(SA)具有抑制作用,可在24h内杀灭96.8％的MRSA,主要作用于细胞膜与细胞壁,增强了细胞膜与细胞壁的通透性,使抗生素更易进入细胞内。本发明的高效复合抑菌剂与现有技术相比,解决了MRSA对常规抗生素的广谱耐药性,并且同时使用两种抑菌物质降低了MRSA对本产品产生耐药性的几率,减少抗生素使用量,提升杀菌效果。(The invention discloses a berberine hydrochloride and levofloxacin high-efficiency composite bacteriostatic agent, belonging to the field of medicines. The efficient composite bacteriostatic agent for the berberine hydrochloride and the levofloxacin is characterized by comprising the following components in percentage by weight when inhibiting methicillin-resistant staphylococcus aureus: 7.2-23.8% of berberine hydrochloride; 0.2-3.1% of levofloxacin; the balance being sterile water. The high-efficiency composite bacteriostatic agent has an inhibiting effect on methicillin-resistant staphylococcus aureus (MRSA) and common Staphylococcus Aureus (SA), can kill 96.8 percent of MRSA within 24 hours, mainly acts on cell membranes and cell walls, enhances the permeability of the cell membranes and the cell walls, and enables antibiotics to enter the cells more easily. Compared with the prior art, the efficient composite bacteriostatic agent solves the problem of broad-spectrum drug resistance of MRSA to conventional antibiotics, and simultaneously uses two bacteriostatic substances to reduce the probability of drug resistance of MRSA to the product, reduce the usage amount of antibiotics and improve the bactericidal effect.)

1. The efficient composite bacteriostatic agent for the berberine hydrochloride and the levofloxacin is characterized by comprising the following components in percentage by weight when inhibiting methicillin-resistant staphylococcus aureus: 7.2-23.8% of berberine hydrochloride; 0.2-3.1% of levofloxacin; the balance being sterile water.

2. The efficient composite bacteriostatic agent for berberine hydrochloride and levofloxacin according to claim 1, which is characterized by comprising the following components in percentage by weight when inhibiting staphylococcus aureus: 7.2-23.8% of berberine hydrochloride; 0.002-0.025% of levofloxacin; the balance being sterile water.

Technical Field

The invention belongs to the field of medicines, and particularly relates to a compound bacteriostatic mixture.

Background

Methicillin-resistant staphylococcus aureus (MRSA) was first discovered in 1961 to make almost all antibiotics ineffective at that time, including the most effective β -lactam drugs, since 1970 MRSA has become a major cause of nosocomial infections worldwide, the only antibiotic effective against it was vancomycin, but vancomycin has ototoxicity and nephrotoxicity and is not suitable as a long-term antibiotic.

Since the advent of antibiotics in the 50's of the 20 th century, plant derivatives have rarely been used as bacteriostats, but interest in the use of phytochemicals (secondary plant metabolites) to treat microbial infections has increased with increased bacterial resistance after 1990. It is widely believed that phytochemicals are largely involved in the defense/stress mechanisms of plants against microorganisms, and that the diversity of phytochemicals can be attributed to the fact that during evolution plants face numerous threats such as microorganisms, insects, nematodes and other plants that require more defense mechanisms. At present, natural antibacterial agents can be classified into plant sources (tea polyphenol and the like), animal sources (propolis, chitosan and the like) and microorganism sources (lysozyme, polylysine and the like) according to source classification, and scholars at home and abroad carry out a great deal of research on the antibacterial activity of natural substance crude extracts at present.

The large-scale commercial use of antibiotics has been for over 70 years, drug-resistant strains are continuously appeared, the trend is difficult to avoid, new challenges are brought to clinical treatment, and the drug resistance of microorganisms is increasingly valued by people. In recent years, with the slow development speed of novel antibacterial drugs, the combination of Chinese herbal medicine extracts and antibiotics has attracted much attention.

However, the key to the problem is to find effective combinations of antibiotics and natural products, optimal ratios and to search for mechanisms of action.

Disclosure of Invention

Aiming at the problems of the combination and the proportion of effective antibiotics and natural products, the inventor screens out the combination of berberine hydrochloride and levofloxacin and the optimal effective proportion thereof from a plurality of antibiotics and natural products which can inhibit Staphylococcus Aureus (SA) by using a trace broth dilution method through a large amount of experiments.

The invention provides the following technical scheme:

the efficient composite bacteriostatic agent for berberine hydrochloride and levofloxacin contains the following components in percentage by weight when the MRSA is inhibited: 7.2-23.8% of berberine hydrochloride; 0.2-3.1% of levofloxacin; the balance being sterile water.

The invention also relates to application of the high-efficiency composite bacteriostatic agent in inhibiting SA, which is characterized by comprising the following components in percentage by weight when the SA is inhibited: 7.2-23.8% of berberine hydrochloride; 0.002-0.025% of levofloxacin; the balance being sterile water.

The invention combines the high efficiency of antibiotics with the characteristics of harmless natural products and difficult drug resistance generation, prevents the abuse of antibiotics, can generate ideal inhibiting effect on MRSA and SA by compounding a small amount of antibiotics and berberine hydrochloride, just accords with the trend of reducing the use of antibiotics and preventing the generation of drug-resistant strains, and has excellent application prospect in the aspects of food safety and prevention and control of drug-resistant bacteria.

Drawings

FIG. 1 is a graph of the bactericidal effect of berberine hydrochloride and its sub-inhibitory concentration groups.

Fig. 2 is a graph showing the sterilization curve of berberine hydrochloride, levofloxacin and the composite bacteriostatic agent.

Fig. 3 is a graph of the content of berberine hydrochloride, levofloxacin and compound bacteriostatic agent AKP.

FIG. 4 is the graph of the electrical conductivity of berberine hydrochloride, levofloxacin and complex bacteriostatic agent.

FIG. 5 is a diagram showing the cell membrane potential curves of berberine hydrochloride, levofloxacin and the complex bacteriostatic agent.

Detailed Description

The strains and reagents used are all conventional commercial strains and reagents.