阅读说明：本技术 一种鳄鱼多肽的提取方法 (Method for extracting crocodile polypeptide ) 是由 张树华 于 2020-05-11 设计创作，主要内容包括：本发明提供一种鳄鱼多肽的提取方法,属于生物样品提取技术领域,其通过对鳄鱼组织进行加热软化,再进行高温恒温水解,再对水解产物进行浓缩干燥得到鳄鱼多肽提取物粉末,具有操作简单、提取效率高和提取物活性高的优点。(The invention provides an extraction method of crocodile polypeptide, belonging to the technical field of biological sample extraction.)

1. The extraction method of the crocodile polypeptide is characterized by comprising the following steps:

step S10, taking crocodile tissues, fully cleaning, and heating and hydrolyzing the cleaned crocodile tissues;

step S20, standing and precipitating the hydrolysate obtained in the step S10, and filtering to obtain a filtrate which is a crude extract;

step S30, adding diatomite and active carbon into the crude extract obtained in the step S20, stirring, standing for precipitation again, and filtering to obtain filtrate as a fine extract;

and step S40, drying the refined extract obtained in step S30 to obtain crocodile peptide powder.

2. The method for extracting crocodile polypeptide according to claim 1, wherein the crocodile tissues in the step S10 comprise crocodile bone, crocodile skin and crocodile meat.

3. The method for extracting crocodile polypeptide according to claim 1, wherein the heating hydrolysis in step S10 is to put crocodile tissue into distilled water and heat and stir in an oil bath at 110-130 ℃ for 7-9 h; the mass ratio of the crocodile tissues to the distilled water is that the crocodile tissues: distilled water 1: 5.

4. the method for extracting crocodile polypeptide according to any one of claims 1-3, wherein the step S10 further comprises a step S11 of soaking crocodile tissue in clear water at 70-80 ℃ for 25-35 min.

5. The method for extracting crocodile polypeptide according to claim 1, wherein the step S40 comprises a step S41, wherein the refined extract obtained in the step S30 is concentrated by a vacuum water pump to obtain a concentrated extract.

6. The method for extracting crocodile polypeptide according to claim 5, wherein the step S40 comprises a step S42, and the concentrated extract obtained in the step S41 is dried into powder by using a freeze dryer.

Technical Field

The invention belongs to the technical field of biological sample extraction, and particularly relates to an extraction method of crocodile polypeptide.

Background

The protein is used as a nutrient essential to human body and is very important to the life health of human body. Epidemiological investigation results show that if the daily protein intake is too low, esophageal cancer, gastric cancer, liver cancer and the like are easy to suffer. Experts in the world health organization and the food and agriculture organization of the United nations consider that a standard diet for normal activities of common citizens cannot bring the protein intake to less than 75g per day. The proteins are classified into animal proteins, plant proteins, single cell proteins and algae proteins according to the source, and the macromolecular proteins supplement proteins required by human bodies to a certain extent, but have the defects of difficult digestion, poor absorption, acid resistance and the like, and some proteins have certain anaphylactic property.

A large number of scientific researches show that a large number of bioactive polypeptides can be obtained by hydrolyzing certain proteins by controlling the hydrolysis degree and the hydrolysis conditions, and the bioactive polypeptides not only have the advantages of high activity, easy digestion and absorption, high safety and the like, but also have special physiological regulation functions, such as reducing blood fat, delaying senility, beautifying the body and keeping young, resisting oxidation, resisting depression, resisting fatigue, improving sleep, enhancing memory, inhibiting tumors and the like; can also promote the absorption of protein, vitamins and various beneficial trace elements by human body. Scientific research finds that active polypeptide in an organism is an important chemical messenger communicating cells and organs, and transmits various specific information through action modes such as endocrine, paracrine, neuroendocrine and neuroendocrine, so that life processes such as growth, development, reproduction, metabolism and behavior can be regulated.

The research field of bioactive polypeptide is developed quickly, has been paid high attention by scientists and governments of various countries, and the bioactive polypeptide is obtained by hydrolyzing or processing food protein, has the unique advantages of rich raw materials, low cost, good safety, convenience for industrial production, good applicability and the like, and is advocated by the science of various countries. The application of the active polypeptide is mainly in two aspects of medical use and eating. The active polypeptide is used as a medicine, and the active action points of the active polypeptide are concentrated on shorter polypeptide chains, so that the effective value and the biological activity of the medicine can be improved, the adverse reaction of non-target active ingredients can be reduced, and the specificity of the active polypeptide is improved. The application of the active polypeptide in the food is mainly concentrated on the functional food, the functional polypeptide health-care food has just started in China, the additional value is high, the market space is huge, and the economic and social benefits are high.

Crocodile belongs to vertebrate reptilia, widely grows in south Asia countries such as Vietnam, Thailand, Malaysia and the like, and has the characteristics of large body size, strong reproductive capacity, fast growth, strong disease resistance and the like as the only existing offspring of grandma. The whole body of the crocodile is precious, and the meat, bones, nails and internal organs of the crocodile contain rich and excellent protein, amino acid, unsaturated fatty acid, vitamins and various trace elements which are necessary for human bodies.

Modern medical research finds that the crocodile meat is delicious, can tonify qi and blood, strengthen bones and muscles, treat asthma, and also has obvious curative effects on chronic cough and diabetes mellitus.

Disclosure of Invention

Based on the current situation, the invention provides the crocodile polypeptide extraction method, which comprises the steps of heating and softening crocodile tissues, carrying out high-temperature constant-temperature hydrolysis, and concentrating and drying hydrolysate to obtain crocodile polypeptide extract powder.

The invention achieves the above purpose through the following detailed technical scheme:

a method for extracting crocodile polypeptide, which comprises the following steps:

step S10, taking crocodile tissues, fully cleaning, and heating and hydrolyzing the cleaned crocodile tissues;

step S20, standing and precipitating the hydrolysate obtained in the step S10, and filtering to obtain a filtrate which is a crude extract;

step S30, adding diatomite and active carbon into the crude extract obtained in the step S20, stirring, standing for precipitation again, and filtering to obtain filtrate as a fine extract;

and step S40, drying the refined extract obtained in step S30 to obtain crocodile peptide powder.

The crocodile tissues in the step S10 comprise crocodile bones, crocodile skins and crocodile meat, and the crocodile bones, the crocodile skins and the crocodile meat all contain a large amount of proteins, so that the extraction of polypeptides is facilitated.

Wherein, the heating hydrolysis in the step S10 is to put the crocodile tissue into distilled water and heat and stir the crocodile tissue in an oil bath kettle at the temperature of 110-130 ℃ for 7-9 h; the mass ratio of the crocodile tissues to the distilled water is that the crocodile tissues: distilled water 1: 5.

wherein the step S10 further comprises a step S11 of soaking crocodile tissues in clear water at 70-80 ℃ for 25-35 min.

Wherein, the step S40 includes a step S41, the refined extract obtained in the step S30 is taken and concentrated by a vacuum water pump to obtain a concentrated extract.

Wherein, the step S40 includes a step S42, and the concentrated extract obtained in the step S41 is dried into powder by using a freeze dryer.

The invention has the following beneficial effects: the extraction method provided by the invention has strong universality and is suitable for crocodile tissues such as crocodile bones, crocodile skin, crocodile meat and the like; the extracted crocodile polypeptide has high activity and obvious activity effect in the aspect of antioxidation.

Detailed Description

The present invention will be described in detail with reference to specific examples.