阅读说明：本技术 用于下一代测序仪的引物及其制备方法，通过使用用于下一代测序仪的引物得到的dna文库及其制备方法，以及使用dna文库的dna分析方法 (Primer for next generation sequencer and method for preparing the same, DNA library obtained by using primer for next generation sequencer and method for preparing the same, and DNA analysis method us) 是由 榎宏征 竹内由枝 稻森稔 于 2018-12-21 设计创作，主要内容包括：本发明提供了用于下一代测序仪的引物,其可以提供大数量的读段。基于序列：5’-CAAGCAGAAGACGGCATACGAGAT-N<Sub>5至15</Sub>-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’(其中N<Sub>5至15</Sub>表示5至15个核苷酸的索引序列),将索引序列设计为表现出推定读段数量超过给定水平的核苷酸序列,其中该推定读段数量根据将读段数量指定为目的变量并将索引序列中的核苷酸类型指定为解释变量的估算公式计算得出。(The present invention provides primers for next generation sequencers that can provide a large number of reads. Based on the sequence: 5' -CAAGCAGAAGACGGCATACGAGAT-N 5 to 15 -GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3' (where N is 5 to 15 Index sequence representing 5 to 15 nucleotides), the index sequence being designed to represent a nucleotide sequence in which the number of putative reads exceeds a given level, wherein the number of putative reads is based on the number of readsThe amount is calculated as an estimate of the formula designating the variable of interest and the nucleotide type in the index sequence as an explanatory variable.)