阅读说明：本技术 一种用于体外诊断的肌酐定量检测干片 (Creatinine quantitative detection dry sheet for in vitro diagnosis ) 是由 黄利寒 郑日记 甘斌 武伟民 李菁杨 于 2019-10-31 设计创作，主要内容包括：本发明提供一种用于体外诊断的肌酐定量检测干片,包括带样品滴加孔的上外壳、扩散层、酶层、色素层、树脂层、支持层、带透光孔的下外壳,其中,上外壳与下外壳相互贴合,上外壳中心位置开有样品滴加孔,下外壳中心位置开有透光孔,扩散层、酶层、色素层、树脂层、支持层通过涂布工艺从上到下依次分层贴合在一起,所述色素层包括色素、缓冲液以及亲水性聚合物,所述酶层包括肌酐氨基水解酶、过氧化物酶、肌氨酸氧化酶、肌酸脒基水解酶以及缓冲液和亲水性聚合物。通过本发明,以解决现有技术存在的荧光光度技术对仪器的要求比较高,在液相中,可以用来检测的分光光度计很贵,且结果不能长期保存的问题。(The invention provides a creatinine quantitative determination dry sheet for in vitro diagnosis, which comprises an upper shell with a sample dripping hole, a diffusion layer, an enzyme layer, a pigment layer, a resin layer, a support layer and a lower shell with a light hole, wherein the upper shell and the lower shell are mutually attached, the sample dripping hole is formed in the center of the upper shell, the light hole is formed in the center of the lower shell, the diffusion layer, the enzyme layer, the pigment layer, the resin layer and the support layer are sequentially attached together in a layering mode from top to bottom through a coating process, the pigment layer comprises pigment, buffer solution and hydrophilic polymer, and the enzyme layer comprises creatinine amidohydrolase, peroxidase, sarcosine oxidase, creatine amidinohydrolase, buffer solution and hydrophilic polymer. The invention solves the problems that the prior fluorescence photometry technology has higher requirements on instruments, a spectrophotometer which can be used for detection is expensive in a liquid phase, and the result can not be stored for a long time.)

1. A creatinine quantitative determination dry plate for in vitro diagnosis is characterized by comprising an upper shell (1) with a sample dripping hole, a diffusion layer (2), an enzyme layer (3), a pigment layer (4), a resin layer (5), a support layer (6) and a lower shell (7) with a light hole, wherein the upper shell (1) and the lower shell (7) are mutually jointed, the center of the upper shell is provided with a sample dripping hole (8), the center of the lower shell is provided with a light hole (9), the diffusion layer (2), the enzyme layer (3), the pigment layer (4), the resin layer (5) and the support layer (6) are sequentially laminated and jointed together from top to bottom through a coating process, the pigment layer comprises pigment, buffer solution and hydrophilic polymer, and the enzyme layer comprises creatinine aminohydrolase, peroxidase, sarcosine oxidase, creatine amidinohydrolase, buffer solution and hydrophilic polymer.

2. The dry plate for quantitative creatinine detection according to claim 1, wherein the diffusion layer (2) comprises a hydrophilic polymer material and a white reflective material, and has a wet film thickness of 200-400 μm and a dry film thickness of 60-300 μm.

3. The dry sheet for quantitative determination of creatinine in vitro diagnosis according to claim 2, wherein said hydrophilic polymer material is cellulose acetate, and said white reflective material is one or a mixture of more than one reflective materials selected from titanium dioxide, barium sulfate, polystyrene, and microcrystalline cellulose.

4. The dry plate for quantitative creatinine measurement according to claim 1, wherein said dye is a leuco dye which is oxidizable to a detectable color dye in the presence of hydrogen peroxide and peroxide, and said leuco dye comprises a diarylimidazole or triarylimidazole; the hydrophilic polymer comprises one or more of gel, gelatin derivative, colla Corii Asini, polyvinyl alcohol, polyvinylpyrrolidone, and polyacrylamide, and the buffer solution can be selected from phosphate buffer solution or citric acid buffer solution.

5. The dry plate for quantitative creatinine detection according to claim 4, wherein said leuco dye is triarylimidazole, and said triarylimidazole is 2- (3, 5-dimethoxy-4-hydroxyphenyl) -4, 5-bis (4-dimethylaminophenyl) imidazole or 2- (4-hydroxy-3-methoxyphenyl) -4, 5-bis (p-dimethylaminophenyl) -1H-imidazole.

6. The dry plate for quantitative determination of creatinine according to claim 1, wherein the wet film thickness of the pigment layer (4) and the enzyme layer (3) is 50 to 300 μm, and the dry film thickness is 20 to 200 μm.

7. The dry plate for quantitative determination of creatinine according to claim 1, wherein said support layer (6) is made of transparent plastic; the thickness of the supporting layer is 30-200 mu m, the light transmittance of the supporting layer in a visible light range is more than 85%, and both incident light source and reflected light can pass through the supporting layer.

8. The dry plate for quantitative determination of creatinine for in vitro diagnosis according to claim 1, wherein said resin layer (5) is a novel resin system using water instead of an organic solvent as a dispersion medium.

9. The creatinine quantitative determination dry plate for in vitro diagnosis according to claim 8, wherein said resin layer (5) is a modified polybutadiene resin; the wet film thickness of the resin layer is 10-100 μm, and the dry film thickness is 2-50 μm.

10. The dry plate for quantitative creatinine measurement according to claim 1, wherein the upper and lower housings (1, 7) are mainly made of plastic material, and are attached together by ultrasonic bonding.

Technical Field

The invention relates to an in-vitro diagnosis detection reagent, in particular to a creatinine quantitative detection dry plate for in-vitro diagnosis.

Background

So-called "dry-patch reagents" are relatively conventional "wet chemistry". The method is a mode that liquid in a detected sample is used as a reaction medium, and an object to be detected directly reacts with a reagent fixed on a carrier, and the method is different from the traditional wet chemistry in the medium participating in the chemical reaction. With the development of techniques for separation, purification, storage, etc. of enzymes in biochemistry, the progress of sensor, photometer, and electrode techniques, and the popularization of computers, dry chemistry has also rapidly developed.

Compared with wet chemistry, the dry chemistry analysis method has the advantages of corresponding detection instruments, simple operation, short detection time, accurate obtained result and the like. The application range of a dry chemical test paper method in clinical examination is obviously expanded, more and more biochemical examination items can be carried out, VITORS 350 proposed by Olson corporation in America is suitable for a full-automatic analysis system for emergency treatment and conventional biochemistry, samples such as serum, plasma, whole blood, urine, cerebrospinal fluid and the like can be detected, the detection items comprise combined items of liver function, kidney function, myocardial enzyme, blood fat, protein, ions and the like or any single item, the rapid, accurate and accurate result can be really realized, the clinical accurate, rapid and flexible diagnosis requirements can be fully met, but a matched instrument is overlarge, and the detection can not be carried out in real time.

With the progress of biotechnology, the immune dry sheet containing enzyme markers and fluorescence markers, especially colloidal gold or selenium-labeled antibodies (or antigens) developed by utilizing technologies such as immunoosmosis, immunochromatography and the like can be used for analytical determination of troponin, special proteins, hormones, certain therapeutic drugs, virus antibodies or antigens and the like, the multilayer dry sheet adopting a fluorescence photometry and a matched instrument thereof are also available and are used more and more, and a vaginitis quintuplet detection reagent of Henan atlas biology company is used for detecting specific biochemical markers by a dry chemical enzyme method and is used for diagnosis of gynecological diseases. After more than 20 years of development, dry chemistry analysis techniques have been widely used to examine various aspects of medicine, including routine biochemistry, endocrine hormone, toxin drug concentration analysis, and special protein immunoassays. However, the requirement of the fluorescence photometry technology on instruments is high, a spectrophotometer capable of being used for detection is expensive in a liquid phase, and the result cannot be stored for a long time; the colloidal gold technology is used as a primary screening diagnostic reagent for the auxiliary treatment of disease diagnosis because the quality of a product is greatly different, the quality cannot be controlled, the quality cannot be ensured, and the phenomenon of the back zone exists, and a specimen needs to be diluted and tested, can only be qualitative and cannot be quantitative.

Disclosure of Invention

The invention provides a creatinine quantitative detection dry sheet for in vitro diagnosis, which aims to solve the problems that the prior art has higher requirements on instruments by a fluorescence photometry technology, a spectrophotometer capable of being used for detection is expensive in a liquid phase, and the result cannot be stored for a long time; and the colloidal gold technology has the problems that the product quality is relatively large in difference, the quality cannot be controlled, the quality cannot be ensured, the phenomenon of back zone exists, a sample needs to be diluted and tested, and only qualitative and quantitative measurement can be carried out.

In order to solve the technical problems, the invention provides a creatinine quantitative determination dry plate for in vitro diagnosis, which comprises an upper shell with a sample dripping hole, a diffusion layer, an enzyme layer, a pigment layer, a resin layer, a support layer and a lower shell with a light transmission hole, wherein the upper shell and the lower shell are mutually attached, the center of the upper shell is provided with the sample dripping hole, the center of the lower shell is provided with the light transmission hole, the diffusion layer, the enzyme layer, the pigment layer, the resin layer and the support layer are sequentially attached in a layered manner from top to bottom through a coating process, the pigment layer comprises a pigment, a buffer solution and a hydrophilic polymer, and the enzyme layer comprises creatinine aminohydrolase, peroxidase, sarcosine oxidase, creatine amidine hydrolase, the buffer solution and the hydrophilic polymer.

The diffusion layer mainly comprises a hydrophilic high polymer material and a white light reflecting material, the wet film thickness of the diffusion layer is 200-400 mu m, and the dry film thickness is 60-300 mu m.

The hydrophilic polymer material is cellulose acetate, and the white reflective material is one or a mixture of more than one reflective material selected from titanium dioxide, barium sulfate, polystyrene and microcrystalline cellulose.

The pigment is a leuco dye which can be oxidized into a detectable colored dye in the presence of hydrogen peroxide and peroxide, and the leuco dye comprises diaryl imidazole or triaryl imidazole; the hydrophilic polymer comprises one or more of gel, gelatin derivative, colla Corii Asini, polyvinyl alcohol, polyvinylpyrrolidone, and polyacrylamide, and the buffer solution can be selected from phosphate buffer solution or citric acid buffer solution.

The leuco dye is triarylimidazole, and the triarylimidazole is 2- (3, 5-dimethoxy-4-hydroxyphenyl) -4, 5-bis (4-dimethylaminophenyl) imidazole or 2- (4-hydroxy-3-methoxyphenyl) -4, 5-bis (p-dimethylaminophenyl) -1H-imidazole.

The wet film thickness of the pigment layer and the enzyme layer is 50-300 mu m, and the dry film thickness is 20-200 mu m.

The supporting layer is made of transparent plastic; the thickness of the supporting layer is 30-200 mu m, the light transmittance of the supporting layer in a visible light range is more than 85%, and both incident light source and reflected light can pass through the supporting layer.

The resin layer is a novel resin system which takes water as a dispersion medium instead of an organic solvent.

The resin layer is modified polybutadiene resin; the wet film thickness of the resin layer is 10-100 mu m, and the dry film thickness is 2-50 mu m.

The upper shell and the lower shell are mainly made of plastic materials, and the upper shell and the lower shell are attached together in an ultrasonic attaching mode.

The invention has the following beneficial effects: the creatinine quantitative detection dry sheet for in vitro diagnosis provided by the invention is a dry chemical method multi-layer membrane reagent dry sheet for in vitro diagnosis, has the advantages of rapid diagnosis, simple operation, cost saving and the like, can improve the accuracy, precision and sensitivity of detection, and has traceability, stable reagent and long storage time.

Drawings

Fig. 1 is a schematic structural diagram of a creatinine quantitative determination dry plate for in vitro diagnosis according to an embodiment of the present invention.

Wherein, 1-upper shell, 2-diffusion layer, 3-enzyme layer, 4-pigment layer, 5-resin layer, 6-support layer, 7-lower shell, 8-sample dropping hole and 9-light hole.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

As shown in figure 1, the invention provides a creatinine quantitative determination dry plate for in vitro diagnosis, which comprises an upper shell 1 with a sample dripping hole, a diffusion layer 2, an enzyme layer 3, a pigment layer 4, a resin layer 5, a support layer 6 and a lower shell 7 with a light hole. Wherein, the upper shell 1 and the lower shell 7 are mutually jointed. The center of the upper shell 1 is provided with a sample dripping hole 8 for dripping a sample, and the center of the lower shell 7 is provided with a light hole 9. The diffusion layer 2, the enzyme layer 3, the pigment layer 4, the resin layer 5, and the support layer 6 are laminated by a coating process.

The invention consists of a module containing dry chemical reagents attached to a rigid plastic strip. The color of the module changes due to the reaction between various chemical examination contents in the blood and dry chemical reagents, and the color depth or the color change speed of the module is in proportional relation with the concentration of corresponding chemical components in the blood and urine.

Furthermore, the creatinine multilayer film dry sheet can be used for quantitatively measuring the concentration of creatinine in serum, plasma and urine. The creatinine dry sheet is a multi-layer analysis component coated on a polyester substrate, a sample is dripped to the surface of a diffusion layer through a sample dripping hole of an upper shell, is uniformly distributed to a reagent layer below through the diffusion layer, creatinine is diffused to the reagent layer to be hydrolyzed into creatine, then is converted into sarcosine and urea through the action of creatine amidinohydrolase, the sarcosine is oxidized into glycine, formaldehyde and hydrogen peroxide under the action of sarcosine oxidase, and finally, a colorless dye generates a colored substance under the catalytic oxidation action of peroxidase. After the sample is added dropwise, the dry plate is incubated, endogenous creatine in the sample is oxidized in the early stage of the reaction, the change of the generated reflection intensity is measured at two time points, and the difference value of the measured reflection intensity is in direct proportion to the concentration of creatinine in the sample. The specific reaction is as follows:

wherein, the diffusion layer mainly comprises hydrophilic polymer material (such as cellulose acetate) and white reflective material (such as titanium dioxide, barium sulfate, polystyrene, microcrystalline cellulose or mixture of the above reflective materials); the diffusion layer has the main functions of uniformly diffusing a sample, filtering macromolecular substances and interfering substances in the sample and protecting a reagent layer; the wet film thickness of the diffusion layer is 200-400 μm, and the dry film thickness is 60-300 μm, and the effect is better within 100-250 μm. The reagent layer is a region where a substance to be detected is subjected to chemical reaction, and is different from other inventions in that the reagent layer comprises a pigment layer and an enzyme layer, wherein the pigment layer comprises necessary biological agents such as necessary pigment, buffer solution, hydrophilic polymer and the like, and the enzyme layer comprises creatinine aminohydrolase, peroxidase, sarcosine oxidase, creatine amidinohydrolase, buffer solution, hydrophilic polymer and the like; the pigment is a leuco dye which is oxidized to a detectable colored dye in the presence of hydrogen peroxide and peroxide. Useful leuco dyes include di-or triarylimidazoles, particularly triarylimidazoles such as 2- (3, 5-dimethoxy-4-hydroxyphenyl) -4, 5-bis (4-dimethylaminophenyl) imidazole, 2- (4-hydroxy-3-methoxyphenyl) -4, 5-bis (p-dimethylaminophenyl) -1H-imidazole and the like; hydrophilic polymers include gelatin (e.g., acid-washed gelatin, deionized gelatin)), gelatin derivatives (e.g., titanates, methyl hydroxymethylacrylate, etc.), gelatin, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylamide, etc., with gelatin being the most preferred. The buffer is added to maintain the enzyme at or near an optimal pH, and may be a phosphate buffer or a citrate buffer. The wet film thickness of the pigment layer and the enzyme layer is 50 to 300 μm, and the dry film thickness is 20 to 200 μm, and the effect is better within 40 to 200 μm. The support layer can be made of various transparent plastics, such as Polyethylene (PE), polypropylene (PP), Polyester Methyl Methacrylate (PMMA), Polystyrene (PS), Polycarbonate (PC), polyethylene terephthalate (PET), and the like, preferably PET; the thickness of the supporting layer is 30-200 μm, preferably 50-150 μm; the light transmittance of the visible light range is more than 85 percent, the optimal light transmittance is more than 95 percent, and the incident light source and the reflected light can both pass through the supporting layer. The supporting layer and the reagent layer coating film can be better adhered together by means of the resin layer with the adhesion function, so that the requirements on the material and the performance of the base material are reduced, and the cost is saved. The resin layer is a novel resin system which takes water as a dispersion medium instead of an organic solvent, and comprises epoxy resin, alkyd resin, polyester resin, polyurethane resin, modified polybutadiene resin and the like, preferably modified polybutadiene resin; the wet film thickness of the resin layer is 10 to 100 μm, and the dry film thickness is 2 to 50 μm, and the effect is better within 10 to 20 μm. The shell mainly comprises the plastics material, the shell is in the same place through the laminating of supersound laminating mode from top to bottom, be fixed in between the shell from top to bottom with the reagent, it is mainly that the protection reagent is in the transportation, store, the in-process of test is because the deformation that various power leads to, thereby avoid the damage of reagent, especially in last quick-witted test, no matter be automatic or semi-automatic send into the detection machine with the dry piece reagent, the dry piece inevitable receives corresponding power, the reagent shell can avoid the dry piece damage of reagent.

The preparation method of the dry tablet reagent comprises the following steps:

1. the resin solution (purchased directly) was uniformly coated on a substrate, dried at 45 ℃ for 10min to form a resin layer of uniform thickness.

2. And continuously coating the pigment layer solution on the dried resin layer, drying for 30min at the temperature of 45 ℃, and forming a pigment layer with uniform thickness after drying. The formula of the pigment layer solution is as follows:

3. and coating the enzyme layer solution on the dried pigment layer, drying for 30min at 45 ℃ to form an enzyme layer with uniform thickness. The formulation of the enzyme layer solution is as follows:

4. and continuously coating the diffusion layer solution on the dried enzyme layer, drying for 5 hours at the temperature of 25 ℃, and completely drying to finish the manufacture of the dry powder dry tablet. The formulation of the diffusion layer solution is as follows;

5. cutting the dry powder sheet into a certain size, and placing the cut dry powder sheet in a dry sheet shell matched with a machine to finish the manufacture of the dry sheet.

The dry chemical reagent prepared by the method has the advantages of excellent performance, stable reagent, long storage time and convenient carrying, and completely realizes the target of immediate detection.

The dry piece detection operation flow is as follows:

1. taking out the dry slices according to the required amount, and returning the temperature for 1 h;

2. the dried tablets were loaded into the test machine and 10ul of whole blood/plasma/urine/cerebrospinal fluid was added drop wise directly to the sample drop on the upper housing of the dried tablet and tested for 5 minutes to yield a result.

And (5) judging a result: referring to the reference value range, the out-of-range is an abnormal value.

In conclusion, the dry chemical method multi-layer membrane reagent dry tablet for in vitro diagnosis provided by the invention has the advantages of rapid diagnosis, simplicity in operation, cost saving and the like, can improve the accuracy, precision and sensitivity of detection, and has traceability, stable reagent and long storage time.

The above description is only an example of the present invention, and is not intended to limit the present invention, and it is obvious to those skilled in the art that various modifications and variations can be made in the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.