阅读说明：本技术 用于两向rplc-sfc色谱的系统和方法 (For two to RPLC-SFC chromatography system and method ) 是由 M·阿尔-萨耶 C·文卡塔拉曼尼 于 2015-10-26 设计创作，主要内容包括：提供了用于分离和/或分析有机化合物的复杂混合物的两向色谱系统和方法。具体地,描述了两向反相液相色谱(RPLC)-超临界流体色谱(SFC)系统,其包括在界面上采集从第一方向色谱中洗脱的分析物、同时使得RPLC流动相通过的捕集柱。来自RPLC维度柱(dimension column)的感兴趣的峰被有效地聚焦为捕集柱上的尖锐浓度脉冲,随后将被注入到第二维度SFC柱上。该系统可用于药物化合物的同时非手性和手性分析。第一维度RPLC分离提供非手性纯度结果,且第二维度SFC分离提供手性纯度结果(对映体过量)。(Provide the bidirectional chromatography system and method for the complex mixture for separating and/or analyzing organic compound.Specifically, two are described to reversed-phase liquid chromatography (RPLC)-supercritical fluid chromatography (SFC) system comprising the analyte eluted from first direction chromatography while the trapping column that RPLC mobile phase is passed through are acquired on interface.Interested peak from RPLC dimension column (dimension column) is effectively focused to the sharp concentration pulse in trapping column, will be then injected on the second dimension SFC column.The system can be used for achirality and chiral analysis while medical compounds.First dimension RPLC separation provides Achiral purity as a result, and the second dimension SFC separation offer chiral purity result (enantiomeric excess).)