阅读说明：本技术 肠道病毒和双埃柯病毒的分子检测 (Molecular detection of enteroviruses and echoviruses ) 是由 P·李 L·奈尔 A·卡斯特罗 M·维斯塔 M·塔布 于 2015-04-28 设计创作，主要内容包括：本文提供了用于在生物样品中确定肠道病毒(enterovirus)和双埃柯病毒(parechovirus)存在或不存在的方法。所述方法涉及使用直接从生物样品扩增而无核苷酸提取步骤来鉴定来自所述病毒的靶核苷酸的存在或不存在,但基本上保持与测定提取核苷酸的方法相同的特异性和敏感性。还提供了使用提供的方法的诊断方法和用于实践所述方法的组合物和试剂盒。(Provided herein are methods for determining the presence or absence of enteroviruses (enteroviruses) and coxoviruses (parecoxivirus) in a biological sample. The methods involve the use of amplification directly from a biological sample without a nucleotide extraction step to identify the presence or absence of a target nucleotide from the virus, but retain substantially the same specificity and sensitivity as the methods of determining extracted nucleotides. Diagnostic methods using the provided methods and compositions and kits for practicing the methods are also provided.)