阅读说明：本技术 一种小白鼠注射药物的制备方法及其应用 (Preparation method and application of mouse injection medicine ) 是由 王砚桐 朱智媛 陈彦冰 蔡爱可 李姝睿 殷煜童 吕欣霖 陈雨麟 于 2021-11-03 设计创作，主要内容包括：本发明公开了一种小白鼠注射药物的制备方法及其应用。本发明中,称取步骤S6中的梧桐花多糖5～10克、氯化钠8～10克、活性炭15克、蒸馏水1000～1200毫升,煮沸药液之后等到冷却药液温度至70-75℃；药液经钛棒过滤器脱炭、0.45μm过滤器回滤20分钟；同时将药液冷却到40-60℃；对步骤S8中制得的药液进行灭菌后,再进行最终的密封灌装,从而结束整个制备过程,泰山梧桐花多糖的加入可以提高免疫抑制小鼠的全身抗体水平,从而提高了小鼠的免疫力,为之后的研究带来了更多的便利,提高了试验的准确性和便利性,提高了该注射药物的多功能性,同时采用了植物成分,更加安全有效,无副作用。(The invention discloses a preparation method and application of a mouse injection medicament. In the invention, 5-10 g of phoenix tree flower polysaccharide, 8-10 g of sodium chloride, 15 g of active carbon and 1000-1200 ml of distilled water in the step S6 are weighed, and after the liquid medicine is boiled, the liquid medicine is cooled to 70-75 ℃; the liquid medicine is decarbonized by a titanium rod filter and filtered back for 20 minutes by a 0.45 mu m filter; simultaneously cooling the liquid medicine to 40-60 ℃; the liquid medicine prepared in the step S8 is sterilized and then is finally sealed and filled, so that the whole preparation process is finished, the added polysaccharides in the Chinese parasol tree flowers can improve the whole body antibody level of the immunosuppressed mice, so that the immunity of the mice is improved, more convenience is brought to later research, the accuracy and convenience of tests are improved, the versatility of the injection medicine is improved, and meanwhile, the plant components are adopted, so that the injection medicine is safer and more effective and has no side effect.)

1. A preparation method and application of a mouse injection medicine are characterized in that: the preparation method of the mouse injection medicine comprises the following steps:

s1, placing the collected phoenix tree flower in a drying oven for drying at 60 ℃, crushing, sieving by a 120-mesh sieve, and weighing 1000 g of phoenix tree flower powder;

s2, carrying out reflux degreasing extraction for 3 times by using a Soxhlet extractor with 12 times of 75% of ether, combining filtrates, and carrying out reduced pressure concentration to obtain a degreased sample;

s3, taking a drier, and drying the degreased sample prepared in the step S2 to obtain a dried degreased sample;

s4, taking the dried and degreased sample in the step S3, adding 5000 g of water, mixing fully, combining for four times, and taking supernatant;

s5, concentrating the supernatant under reduced pressure, adding ethanol for precipitation, washing and drying to obtain a crude polysaccharide sample;

s6, taking a crude polysaccharide sample, adding a proper amount of distilled water, deproteinizing and dialyzing to obtain the precise phoenix tree flower polysaccharide;

s7, weighing 5-10 g of phoenix tree flower polysaccharide, 8-10 g of sodium chloride, 15 g of active carbon and 1000-1200 ml of distilled water in the step S6, boiling the liquid medicine, and cooling the liquid medicine to 70-75 ℃;

s8, decarburizing the liquid medicine by a titanium rod filter, and filtering the liquid medicine for 20 minutes by a 0.45-micrometer filter; simultaneously cooling the liquid medicine to 40-60 ℃;

and S9, sterilizing the liquid medicine prepared in the step S8, and finally sealing and filling to finish the whole preparation process.

2. The preparation method and the application of the mouse injection drug as claimed in claim 1, characterized in that: in step S2, the time for each reflux extraction was 3 hours, and the reaction temperature in the reflux extraction was controlled at 40 ℃.

3. The preparation method and the application of the mouse injection drug as claimed in claim 1, characterized in that: in the step S3, the temperature of the dryer is set at 75 ℃; the drying time is 30min, and it can be stirred during the drying process.

4. The preparation method and the application of the mouse injection drug as claimed in claim 1, characterized in that: in the step S5, the washing is performed with a mixed solution of ethanol, acetone and diethyl ether, the washing temperature is controlled at 45 ℃, and the washing time is 1 hour.

5. The preparation method and the application of the mouse injection drug as claimed in claim 1, characterized in that: in step S7, the boiling of the mixed chemical liquid needs to be maintained for 10 minutes.

6. The preparation method and the application of the mouse injection drug as claimed in claim 1, characterized in that: the time for dispensing the liquid medicine in the steps S7 and S8 is controlled within 2.5 hours.

7. The preparation method and the application of the mouse injection drug as claimed in claim 1, characterized in that: in the step S9, the sterilization temperature is 117 ℃, the constant temperature time is 35 minutes, and the cooling temperature is 40-60 ℃; the operation time from the end of washing, filling and sealing to the beginning of sterilization is not more than 1.5 hours.

Technical Field

The invention belongs to the technical field of injection medicines, and particularly relates to a preparation method and application of a mouse injection medicine.

Background

White rats are commonly called mice and chinchillas, are famous for pure white color, have white hair, have tail length basically equal to body length, and have flaky scales attached to the surface of the tail. White rats are the most common experimental animals and account for more than 90% of experimental animals. It has wide application. The contribution of white mice in experiments cannot be avoided, so that the white mice are human candidates and live reagents for scientific research, and the white mice need to be injected with medicaments to improve the immunity in the daily feeding process.

However, the common injection drugs are mostly prepared from chemical preparations for improving the immunity of mice, and the health of the mice is damaged to a certain extent.

Disclosure of Invention

The invention aims to: in order to solve the problems, a preparation method and application of a mouse injection medicament are provided.

The technical scheme adopted by the invention is as follows: a preparation method of a mouse injection medicine and application thereof are provided, the preparation method of the mouse injection medicine comprises the following steps:

s1, placing the collected phoenix tree flower in a drying oven for drying at 60 ℃, crushing, sieving by a 120-mesh sieve, and weighing 1000 g of phoenix tree flower powder;

s2, carrying out reflux degreasing extraction for 3 times by using a Soxhlet extractor with 12 times of 75% of ether, combining filtrates, and carrying out reduced pressure concentration to obtain a degreased sample;

s3, taking a drier, and drying the degreased sample prepared in the step S2 to obtain a dried degreased sample;

s4, taking the dried and degreased sample in the step S3, adding 5000 g of water, mixing fully, combining for four times, and taking supernatant;

s5, concentrating the supernatant under reduced pressure, adding ethanol for precipitation, washing and drying to obtain a crude polysaccharide sample;

s6, taking a crude polysaccharide sample, adding a proper amount of distilled water, deproteinizing and dialyzing to obtain the precise phoenix tree flower polysaccharide;

s7, weighing 5-10 g of phoenix tree flower polysaccharide, 8-10 g of sodium chloride, 15 g of active carbon and 1000-1200 ml of distilled water in the step S6, boiling the liquid medicine, and cooling the liquid medicine to 70-75 ℃;

s8, decarburizing the liquid medicine by a titanium rod filter, and filtering the liquid medicine for 20 minutes by a 0.45-micrometer filter; simultaneously cooling the liquid medicine to 40-60 ℃;

and S9, sterilizing the liquid medicine prepared in the step S8, and finally sealing and filling to finish the whole preparation process.

In a preferred embodiment, in step S2, the time for each reflux extraction is 3 hours, and the reaction temperature in the reflux extraction is controlled at 40 ℃.

In a preferred embodiment, in the step S3, the temperature of the dryer is set at 75 ℃; the drying time is 30min, and it can be stirred during the drying process.

In a preferred embodiment, in step S5, the washing is performed by using a mixture of ethanol, acetone and diethyl ether, the washing temperature is controlled at 45 ℃, and the washing time is 1 hour.

In a preferred embodiment, in step S7, the mixed chemical solution is boiled and then kept boiled for 10 minutes.

In a preferred embodiment, the time for dispensing the liquid medicine in the steps S7 and S8 is controlled within 2.5 hours.

In a preferred embodiment, in the step S9, the sterilization temperature is 117 ℃, the constant temperature time is 35 minutes, and the cooling temperature is 40-60 ℃; the operation time from the end of washing, filling and sealing to the beginning of sterilization is not more than 1.5 hours.

In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:

in the invention, the addition of the harlequin glorybower leaf and twig polysaccharide can improve the whole body antibody level of an immunosuppressed mouse, thereby improving the immunity of the mouse, bringing more convenience for the subsequent research, improving the accuracy and convenience of the test, improving the versatility of the injection medicament, and simultaneously adopting plant components, being safer and more effective and having no side effect.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example (b):

a preparation method of a mouse injection medicine and application thereof are provided, the preparation method of the mouse injection medicine comprises the following steps:

s1, placing the collected phoenix tree flower in a drying oven for drying at 60 ℃, crushing, sieving by a 120-mesh sieve, and weighing 1000 g of phoenix tree flower powder;

s2, carrying out reflux degreasing extraction for 3 times by using a Soxhlet extractor with 12 times of 75% of ether, combining filtrates, and carrying out reduced pressure concentration to obtain a degreased sample; in the step S2, the time of each reflux extraction is 3 hours, and the reaction temperature in the reflux extraction is controlled at 40 ℃;

s3, taking a drier, and drying the degreased sample prepared in the step S2 to obtain a dried degreased sample; in step S3, the temperature of the dryer is set at 75 ℃; the drying time is 30min, and the drying process can be carried out by stirring;

s4, taking the dried and degreased sample in the step S3, adding 5000 g of water, mixing fully, combining for four times, and taking supernatant;

s5, concentrating the supernatant under reduced pressure, adding ethanol for precipitation, washing and drying to obtain a crude polysaccharide sample; in step S5, washing with a mixed solution of ethanol, acetone and diethyl ether, wherein the washing temperature is controlled at 45 ℃ and the washing time is 1 hour;

s6, taking a crude polysaccharide sample, adding a proper amount of distilled water, deproteinizing and dialyzing to obtain the precise phoenix tree flower polysaccharide;

s7, weighing 5 g of phoenix tree flower polysaccharide, 8 g of sodium chloride, 15 g of active carbon and 1000 ml of distilled water in the step S6, boiling the liquid medicine, and waiting until the temperature of the liquid medicine is cooled to 70-75 ℃; in step S7, boiling the mixed liquid medicine is maintained for 10 minutes;

s8, decarburizing the liquid medicine by a titanium rod filter, and filtering the liquid medicine for 20 minutes by a 0.45-micrometer filter; simultaneously cooling the liquid medicine to 40-60 ℃; the time for preparing the liquid medicine in the steps S7 and S8 is controlled within 2.5 hours;

s9, sterilizing the liquid medicine prepared in the step S8, and finally sealing and filling so as to finish the whole preparation process; in step S9, sterilizing at 117 deg.C for 35 min, and cooling at 40-60 deg.C; the time from the completion of washing and filling to the start of sterilization is not more than 1.5 hours, and the addition of the harlequin glorybower herb polysaccharide can improve the whole body antibody level of an immunosuppressive mouse, so that the immunity of the mouse is improved, more convenience is brought to later research, the accuracy and convenience of tests are improved, and the versatility of the injection medicine is improved.

Example two:

a preparation method of a mouse injection medicine and application thereof are provided, the preparation method of the mouse injection medicine comprises the following steps:

s1, placing the collected phoenix tree flower in a drying oven for drying at 60 ℃, crushing, sieving by a 120-mesh sieve, and weighing 1000 g of phoenix tree flower powder;

s2, carrying out reflux degreasing extraction for 3 times by using a Soxhlet extractor with 12 times of 75% of ether, combining filtrates, and carrying out reduced pressure concentration to obtain a degreased sample; in the step S2, the time of each reflux extraction is 3 hours, and the reaction temperature in the reflux extraction is controlled at 40 ℃;

s3, taking a drier, and drying the degreased sample prepared in the step S2 to obtain a dried degreased sample; in step S3, the temperature of the dryer is set at 75 ℃; the drying time is 30min, and the drying process can be carried out by stirring;

s4, taking the dried and degreased sample in the step S3, adding 5000 g of water, mixing fully, combining for four times, and taking supernatant;

s5, concentrating the supernatant under reduced pressure, adding ethanol for precipitation, washing and drying to obtain a crude polysaccharide sample; in step S5, washing with a mixed solution of ethanol, acetone and diethyl ether, wherein the washing temperature is controlled at 45 ℃ and the washing time is 1 hour;

s6, taking a crude polysaccharide sample, adding a proper amount of distilled water, deproteinizing and dialyzing to obtain the precise phoenix tree flower polysaccharide;

s7, weighing 6 g of phoenix tree flower polysaccharide, 9 g of sodium chloride, 15 g of active carbon and 1000 ml of distilled water in the step S6, boiling the liquid medicine, and waiting until the temperature of the liquid medicine is cooled to 70-75 ℃; in step S7, boiling the mixed liquid medicine is maintained for 10 minutes;

s8, decarburizing the liquid medicine by a titanium rod filter, and filtering the liquid medicine for 20 minutes by a 0.45-micrometer filter; simultaneously cooling the liquid medicine to 40-60 ℃; the time for preparing the liquid medicine in the steps S7 and S8 is controlled within 2.5 hours;

s9, sterilizing the liquid medicine prepared in the step S8, and finally sealing and filling so as to finish the whole preparation process; in step S9, sterilizing at 117 deg.C for 35 min, and cooling at 40-60 deg.C; the time from the completion of washing and filling to the start of sterilization is not more than 1.5 hours, and the addition of the harlequin glorybower herb polysaccharide can improve the whole body antibody level of an immunosuppressive mouse, so that the immunity of the mouse is improved, more convenience is brought to later research, the accuracy and convenience of tests are improved, and the versatility of the injection medicine is improved.

Example three:

a preparation method of a mouse injection medicine and application thereof are provided, the preparation method of the mouse injection medicine comprises the following steps:

s1, placing the collected phoenix tree flower in a drying oven for drying at 60 ℃, crushing, sieving by a 120-mesh sieve, and weighing 1000 g of phoenix tree flower powder;

s2, carrying out reflux degreasing extraction for 3 times by using a Soxhlet extractor with 12 times of 75% of ether, combining filtrates, and carrying out reduced pressure concentration to obtain a degreased sample; in the step S2, the time of each reflux extraction is 3 hours, and the reaction temperature in the reflux extraction is controlled at 40 ℃;

s3, taking a drier, and drying the degreased sample prepared in the step S2 to obtain a dried degreased sample; in step S3, the temperature of the dryer is set at 75 ℃; the drying time is 30min, and the drying process can be carried out by stirring;

s4, taking the dried and degreased sample in the step S3, adding 5000 g of water, mixing fully, combining for four times, and taking supernatant;

s5, concentrating the supernatant under reduced pressure, adding ethanol for precipitation, washing and drying to obtain a crude polysaccharide sample; in step S5, washing with a mixed solution of ethanol, acetone and diethyl ether, wherein the washing temperature is controlled at 45 ℃ and the washing time is 1 hour;

s6, taking a crude polysaccharide sample, adding a proper amount of distilled water, deproteinizing and dialyzing to obtain the precise phoenix tree flower polysaccharide;

s7, weighing 7 g of phoenix tree flower polysaccharide, 10 g of sodium chloride, 15 g of active carbon and 1100 ml of distilled water in the step S6, boiling the liquid medicine, and waiting until the temperature of the liquid medicine is cooled to 70-75 ℃; in step S7, boiling the mixed liquid medicine is maintained for 10 minutes;

s8, decarburizing the liquid medicine by a titanium rod filter, and filtering the liquid medicine for 20 minutes by a 0.45-micrometer filter; simultaneously cooling the liquid medicine to 40-60 ℃; the time for preparing the liquid medicine in the steps S7 and S8 is controlled within 2.5 hours;

s9, sterilizing the liquid medicine prepared in the step S8, and finally sealing and filling so as to finish the whole preparation process; in step S9, sterilizing at 117 deg.C for 35 min, and cooling at 40-60 deg.C; the time from the completion of washing and filling to the start of sterilization is not more than 1.5 hours, and the addition of the harlequin glorybower herb polysaccharide can improve the whole body antibody level of an immunosuppressive mouse, so that the immunity of the mouse is improved, more convenience is brought to later research, the accuracy and convenience of tests are improved, and the versatility of the injection medicine is improved.

Example four:

a preparation method of a mouse injection medicine and application thereof are provided, the preparation method of the mouse injection medicine comprises the following steps:

s1, placing the collected phoenix tree flower in a drying oven for drying at 60 ℃, crushing, sieving by a 120-mesh sieve, and weighing 1000 g of phoenix tree flower powder;

s2, carrying out reflux degreasing extraction for 3 times by using a Soxhlet extractor with 12 times of 75% of ether, combining filtrates, and carrying out reduced pressure concentration to obtain a degreased sample; in the step S2, the time of each reflux extraction is 3 hours, and the reaction temperature in the reflux extraction is controlled at 40 ℃;

s3, taking a drier, and drying the degreased sample prepared in the step S2 to obtain a dried degreased sample; in step S3, the temperature of the dryer is set at 75 ℃; the drying time is 30min, and the drying process can be carried out by stirring;

s4, taking the dried and degreased sample in the step S3, adding 5000 g of water, mixing fully, combining for four times, and taking supernatant;

s5, concentrating the supernatant under reduced pressure, adding ethanol for precipitation, washing and drying to obtain a crude polysaccharide sample; in step S5, washing with a mixed solution of ethanol, acetone and diethyl ether, wherein the washing temperature is controlled at 45 ℃ and the washing time is 1 hour;

s6, taking a crude polysaccharide sample, adding a proper amount of distilled water, deproteinizing and dialyzing to obtain the precise phoenix tree flower polysaccharide;

s7, weighing 7 g of Firmiana simplex, 10 g of sodium chloride, 15 g of activated carbon and 1200 ml of distilled water in the step S6, boiling the liquid medicine, and waiting until the temperature of the liquid medicine is cooled to 70-75 ℃; in step S7, boiling the mixed liquid medicine is maintained for 10 minutes;

s8, decarburizing the liquid medicine by a titanium rod filter, and filtering the liquid medicine for 20 minutes by a 0.45-micrometer filter; simultaneously cooling the liquid medicine to 40-60 ℃; the time for preparing the liquid medicine in the steps S7 and S8 is controlled within 2.5 hours;

s9, sterilizing the liquid medicine prepared in the step S8, and finally sealing and filling so as to finish the whole preparation process; in step S9, sterilizing at 117 deg.C for 35 min, and cooling at 40-60 deg.C; the time from the completion of washing and filling to the start of sterilization is not more than 1.5 hours, and the addition of the harlequin glorybower herb polysaccharide can improve the whole body antibody level of an immunosuppressive mouse, so that the immunity of the mouse is improved, more convenience is brought to later research, the accuracy and convenience of tests are improved, and the versatility of the injection medicine is improved.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.