阅读说明：本技术 一种黄芪多糖组合物及其在抗癌药物中的应用 (Astragalus polysaccharide composition and application thereof in anticancer drugs ) 是由 陈新燊 于 2021-01-15 设计创作，主要内容包括：本发明公开了一种黄芪多糖组合物及其在抗癌药物中的应用,本发明能够有机地结合黄芪多糖和大豆小分子肽的特点,充分利用黄芪多糖大豆小分子肽对人体的有益作用,提高人体免疫力,从而具有有效的抗癌作用。(The invention discloses an astragalus polysaccharide composition and application thereof in anticancer drugs, which can organically combine the characteristics of astragalus polysaccharide and soybean small molecular peptide, fully utilize the beneficial effect of the astragalus polysaccharide and the soybean small molecular peptide on human bodies, improve the immunity of the human bodies and have effective anticancer effect.)

1. An astragalus polysaccharide composition is characterized by comprising astragalus polysaccharide and soybean micromolecule peptide.

2. The astragalus polysaccharide composition of claim 1, wherein the ratio of the astragalus polysaccharide to the soybean small molecule peptide is 1-1.5: 1.

3. an application of Astragalus polysaccharides composition in anticancer medicine is provided.

4. The use according to claim 3, wherein the medicament is in the form of any one of tablets, pills, granules, powders, capsules, syrups, emulsions, injections or suspensions.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a novel astragalus polysaccharide composition and application thereof in anticancer drugs.

Background

Medically, cancer (cancer) refers to a malignant tumor that originates in epithelial tissue. Cancer has the biological characteristics of abnormal cell differentiation and proliferation, uncontrolled growth, infiltrative and metastatic properties and the like, is a multi-factor and multi-step complex process, is divided into three processes of carcinogenesis, cancer promotion and evolution, and is closely related to smoking, infection, occupational exposure, environmental pollution, unreasonable diet and genetic factors.

Malignant tumors are diverse in their nature, different in involved tissues and organs, different in disease stage, and different in response to various treatments. The current mainstream treatment means is to adopt the methods of operation, chemotherapy and radiotherapy for treatment, and particularly for patients with advanced cancer, the methods of operation, chemotherapy and radiotherapy are combined together for treatment. The above-mentioned western medicine treatment idea is to remove the focus part and kill cancer cells, thus eliminating the pathological changes, however, the fact proves that this treatment method can not completely eliminate cancer cells and pathological changes, but rather greatly reduces the immunity of the human body to some extent, and has great side effects.

In recent years, the traditional Chinese medicine intervenes in the treatment of cancer, and provides a new means and direction for people, the traditional Chinese medicine treatment is based on the purpose of providing human immunity, and the treatment can greatly improve the curative effect and improve the life quality of patients without the means of surgery, chemotherapy, radiotherapy and the like.

Radix astragali has effects of invigorating qi, invigorating yang, consolidating superficial resistance, arresting sweating, removing toxic substance, promoting granulation, inducing diuresis, and relieving swelling. Modern medicine finds that the pharmacological action is very wide, and the medicine not only can enhance the immune function of an organism, enhance the cell metabolism, regulate the DNA replication and the synthesis of RNA and protein, but also has the functions of reinforcing the kidney, reducing the blood pressure, protecting the liver and resisting inflammation. Astragalus polysaccharides is one of the main active components of Astragalus, can be used as immunopotentiator or regulator, and has antiviral, antitumor, antiaging, radioprotective, anti-stress, and antioxidant effects. At present, astragalus polysaccharide is continuously applied to the health care of livestock and poultry. The beef cattle anti-stress compound premix disclosed in the patent application 201810919135.6 and the astragalus polysaccharide injection are used for treating hyperpyrexia, unknown high fever and fever caused by various virus infections of various animals.

In 2007, Chuaili and Zhujiang in the text of the research status quo and progress of astragalus polysaccharide, a large number of preclinical experiments show that astragalus polysaccharide has the effects of regulating the immunity of organisms, improving the proliferation reaction of T, B lymphocytes of tumor-bearing mice, promoting spleen cells to generate IL-2 and recovering to a normal level, so that the cellular immune function is improved, and the anti-tumor effect is achieved.

In 2013, Gegagaochilu et al reviewed the immunomodulatory activity of Astragalus polysaccharides in terms of immune organs, immune cells, and related cytokines and gene expression in the study progress of Astragalus polysaccharides.

Numerous documents show that the main roles of astragalus polysaccharides are: 1. the feed additive has the advantages of improving the immunity of organisms, preventing and treating the immune system syndrome of livestock and poultry, promoting the growth of animals, improving the appetite of chickens, enhancing the digestion, improving the disease resistance, improving the survival rate, improving the feed conversion rate by 9.0 percent and 10.1 percent, remarkably improving the laying rate of laying hens, reducing the feed-egg ratio and the death rate, and improving the production performance of the laying hens in the egg laying peak period. 2. The astragalus polysaccharide can block the adhesion of pseudomonas aeruginosa on respiratory epithelium, has a repairing effect on the tracheal mucosal epithelium of a mouse with chronic bronchial lesion, and can play a role in resisting pseudomonas aeruginosa infection. 3. The astragalus polysaccharide has comprehensive enhancing effect on cellular immunity, humoral immunity, molecular immunity, complement immunity and the like, has prevention effect on patients with common cold, has the efficacy of strengthening body resistance and expelling toxin on patients with chronic infection, but can activate antibodies, so that the titers of liver enzymes, such as rheumatoid arthritis, Behcet disease oral ulcer, uveitis, polymyositis and immune liver diseases ALT, AST, CK and the like, are obviously increased, and the heat degree of adult still disease is difficult to reduce.

Experiments show that the immunity enhancing function of the astragalus polysaccharide is embodied in the following aspects: (1) the astragalus polysaccharide which affects immune organs can obviously increase the weight of the spleen of a mouse by injection and obviously antagonize the atrophy of the spleen, thymus, lymph nodes and the like of the mouse caused by methylprednisolone (prednisolone). (2) The astragalus decoction can affect macrophages and leukocytes, can obviously promote the number of macrophages in liver, spleen, lung and abdominal cavity and activate phagocytosis activity, and can induce interleukin-1 (IL-1). The intraperitoneal injection of the astragalus can obviously prevent and treat the reduction of the peripheral white blood cell number and the bone marrow megakaryocyte number of the rat caused by cyclophosphamide. (3) The astragalus polysaccharide can promote the differentiation and maturation of T lymphocytes and obviously improve the activity of the T lymphocytes by enhancing the T cells, so that the percentage of the T cells in the thymus is increased, and the functions of T cell subsets and helper T cells are enhanced. And can completely antagonize the inhibition of methylprednisolone (prednisolone) or cyclophosphamide on the functions of T cell subsets and helper T cells. Astragalus has biphasic regulating effect on T cells, reduces inhibitory activity of inhibitory T cells (Ts), and enhances cell activity of helper T cells (Th). (4) The astragalus decoction can induce gamma-interferon, has obvious antiviral activity and can promote mouse L9290 tumor to induce interferon. The capability of peripheral blood leucocyte on virus-induced interferon is obviously improved when astragalus is used for treating patients with cold. The treatment of viral myocarditis with astragalus membranaceus can result in significant increase in alpha and gamma interferon. The astragalus root and the interferon have a synergistic effect on promoting the activity of NK cells. Astragalus polysaccharides can activate interferon system, and induce interferon in lymphocyte. (5) The radix astragali has the effects of enhancing humoral immunity, promoting B cell proliferation, and producing immunoglobulin. The normal person can take radix astragali to obviously improve the content of IgM and IgE. The content of IgG and IgA in nasal secretion of a patient with cold can be obviously improved. Astragalus root can increase the high IgG of patients with spleen deficiency, but also can reduce the high IgG of patients with hepatitis and nephritis to the normal range, which indicates that the astragalus root has the function of regulating antibodies.

However, the above-mentioned research only stays in the research stage, and there is no practical measure, so that the astragalus polysaccharides cannot be well applied; secondly, the above research footholds still analyze and utilize traditional Chinese medicine from the point of view of western medicine, and although there is a certain rationality, the analysis of the action mechanism of traditional Chinese medicine can not get accurate and comprehensive reaction.

Disclosure of Invention

In view of the defects of the prior art, the invention provides the astragalus polysaccharide composition and the application thereof in anticancer drugs, the astragalus polysaccharide composition can organically combine the characteristics of the astragalus polysaccharide and the soybean micromolecule peptide, fully utilize the beneficial effect of the astragalus polysaccharide and the soybean micromolecule peptide on human bodies, improve the immunity of the human bodies, and have effective anticancer effect.

The invention also aims to provide an astragalus polysaccharide composition and application thereof in anticancer drugs, and the astragalus polysaccharide composition can play an effective amplification effect on astragalus polysaccharide through soybean small molecular peptide and increase the anticancer curative effect.

From the perspective of traditional Chinese medicine, astragalus membranaceus is sweet in taste and slightly warm in nature; it enters spleen and lung meridians. For: tonify qi, strengthen superficies, expel toxin, expel pus, induce diuresis, and promote granulation. Can be used for treating deficiency of vital energy, asthenia, chronic diarrhea, proctoptosis, spontaneous perspiration, edema, uterine prolapse, albuminuria due to chronic nephritis, diabetes, and unhealed wound. Astragalus polysaccharides, as an extract of Astragalus membranaceus, naturally have the above-mentioned basic functions. However, in the case of traditional Chinese medicine, the therapeutic principle is to strengthen the body resistance and eliminate pathogenic factors, wherein pathogenic factors predominate over healthy qi to cause progressive disease, and healthy qi predominates over weakened disease. Therefore, the immunity of the human body is improved by combining the treatment aiming at the pathogeny and the condition of the human body for proper supplement and supplementing, and changing the treatment into western medicine, so that the effective treatment on the disease can be realized, the self function of the human body is promoted to be improved, and the treatment purpose can be achieved by matching the action of the medicine. Therefore, generally speaking, the compatibility of the traditional Chinese medicines is designed, and the monarch, minister, assistant and guide combination is designed, that is, the traditional Chinese medicines need to be used in combination with multiple components to achieve effective treatment effects (for example, the single formula is used in combination with a unique using method to achieve the purpose of treating diseases, for example, semen cassiae is used for treating headache in the prescription of plucking yuan, and the semen cassiae needs to be stir-fried and ground, blended with tea and then coated on temple).

Based on the above thought, the invention is realized as follows:

an astragalus polysaccharide composition is characterized by comprising astragalus polysaccharide and soybean micromolecule peptide.

Soybean peptide (Soy peptide) refers to short for peptidyl soybean protein hydrolysate, and is derived from soybean protein enzymolysis product. The soybean polypeptide is a low peptide mixture consisting of 3-6 amino acids, and the molecular weight is 1000 or less, so the soybean polypeptide is also called as soybean micromolecular peptide. The soybean micromolecule peptide can quickly supplement human body nitrogen source, recover physical strength, relieve fatigue, and has the functions of low antigenicity, inhibiting cholesterol, promoting lipid metabolism, fermentation and the like. The research of the applicant finds that the soybean small molecular peptide is not only suitable for people with poor protein digestion and absorption, but also has the effects of improving immunity, enhancing physical strength, relieving fatigue, reducing high blood pressure, high blood sugar and high blood fat and the like.

The traditional using method has independent use and does not have special relation, but the applicant researches and discovers that the two methods have special efficacy by combining the two methods, not only has certain anticancer effect, but also can effectively inhibit the growth of cancer cells and eliminate the cancer cells. The soybean small molecular peptide is composed of amino acids including arginine, glutamic acid and other substances, and has small molecular structures, so that the soybean small molecular peptide is convenient to absorb and has the effects of enhancing immunity, resisting oxidation and delaying senescence. In particular, in the process of enhancing immunity, resisting oxidation and delaying senility, the activity of SOD enzyme and glutathione peroxidase GSH-Px in red blood cells is increased, which is exactly required when astragalus polysaccharide is used for generating the effect. The activities of SOD enzyme, glutathione peroxidase GSH-Px have excellent additive effects on the number of macrophages in liver, spleen, lung and abdominal cavity and activation phagocytic activity, thereby greatly promoting the anticancer ability of human body.

According to the mass, the ratio of the astragalus polysaccharide to the soybean small molecular peptide is 1-1.5: 1. in the proportion, the astragalus polysaccharide and the soybean micromolecule peptide can optimally play a role in matching, and have good inhibition and elimination effects on cancers.

An application of Astragalus polysaccharides composition in anticancer medicine is provided.

The dosage form of the medicine is any one of tablets, pills, granules, powder, capsules, syrup, emulsion, injection or suspension.

The invention fully exerts the immune performance of the astragalus polysaccharide and the soybean micromolecule peptide by combining the astragalus polysaccharide and the soybean micromolecule peptide, and has the function of resisting cancer while enhancing the human immunity.

Experiments prove that the soybean micromolecule peptide has an effective amplification effect on the astragalus polysaccharide and an anti-cancer curative effect. Specifically, the soybean small molecular peptide can increase the activity of SOD enzyme and glutathione peroxidase GSH-Px in red blood cells; the activities of SOD enzyme and glutathione peroxidase GSH-Px have excellent addition performance on the number of macrophages in liver, spleen, lung and abdominal cavity and activation phagocytosis activity, thereby greatly promoting the anti-cancer capability of human body.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The astragalus polysaccharide composition realized by the invention comprises astragalus polysaccharide and soybean micromolecule peptide. Through the combined action of the two components, the traditional Chinese medicine composition not only has a certain anticancer effect, but also can effectively inhibit the growth of cancer cells and eliminate the cancer cells.

According to the mass, the ratio of the astragalus polysaccharide to the soybean small molecular peptide is 1-1.5: 1. in the proportion, the astragalus polysaccharide and the soybean micromolecule peptide can optimally play a role in matching, and have good inhibition and elimination effects on cancers.

An application of Astragalus polysaccharides composition in anticancer medicine is provided.

The dosage form of the medicine is any one of tablets, pills, granules, powder, capsules, syrup, emulsion, injection or suspension.

The soybean small molecular peptide can be prepared by high-temperature fermentation, and the astragalus polysaccharide is extracted by a decoction method or a water extraction and alcohol precipitation method.

And (5) carrying out a first test.

The test was carried out using 12 mice, divided into two groups of 6 mice, one group using a saline feed as a control group and the other group using an astragalus polysaccharide composition. Continuously inoculating 2X 10 tumor cells of S180 cells for 7 days⁶And/ml, continuously feeding with a composition of normal saline and astragalus polysaccharide, killing after 7 days, and carrying out abdominal cavity cell chemotaxis observation and abdominal cavity in vitro phagocytosis test. The comparison of the number of peritoneal cells is shown in Table 1, and the phagocytosis of peritoneal cells is shown in Table 2.

TABLE 1 Abdominal cell count of mice in each group

Table 1 demonstrates that the astragalus polysaccharide composition can promote chemotaxis and aggregation of cells to inflammatory parts, and increase cell number detail.

TABLE 2 phagocytic potency of the peritoneal macrophages and INF α production levels in each group

Table 2 shows that the astragalus polysaccharide composition can greatly enhance the phagocytic ability of macrophages in abdominal cavities of mice and induce the macrophages to generate INF alpha.

And (5) testing II.

The test was carried out using 16 mice, divided into two groups of 8 mice, one group using a saline feed as a control group and the other group using an astragalus polysaccharide composition. 0.2ml of S180 tumor cells (5X 10 dosage unit) were inoculated separately⁵) The relative area of tumor necrosis was calculated by a dot counting method after necropsy by measuring the tumor diameter and calculating the tumor volume after dissection, starting on the day of inoculation and ending on day 6 and day 11 of the experiment. The volume of the tumors in each group is shown in Table 3, and the area of hemorrhagic necrosis of the tumors in each group is shown in Table 4.

TABLE 3 comparison of the volume of tumors (mm) in each group³，X±S)

In the astragalus polysaccharide group, the tumor volume is less than half of that of the control group, and the tumor volume tends to shrink along with the increase of the using time.

TABLE 4 comparison of the areas of hemorrhagic necrosis (mm) of the tumors in each group²)

The tumor area of the astragalus polysaccharide group is far smaller than that of the control group, and the tumor area also tends to shrink along with the prolonging of the use time; the necrotic area of tumor cells was also much larger than the control group.

Therefore, it is understood that the astragalus polysaccharide composition has a detailed cancer cell growth-inhibiting effect and can eliminate cancer cells to some extent.

Case 1.

After the later stage of gastric cancer is checked, the astragalus polysaccharide composition is orally taken, one dose of 100ml is taken every day, and the cancer cell area is greatly reduced after 2 years of reexamination.

In a word, the invention can give full play to the performances of the astragalus polysaccharide and the soybean micromolecular peptide through the combination of the astragalus polysaccharide and the soybean micromolecular peptide, and has effective anticancer effect while enhancing the human immunity. In particular, the soybean micromolecule peptide can play an amplification role in astragalus polysaccharide and has good inhibition and elimination effects on cancers.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.