阅读说明：本技术 一种样品快速提取净化装置 (Sample draws purifier fast ) 是由 李平 曹进 孙姗姗 董喆 路勇 于 2020-12-22 设计创作，主要内容包括：本发明公开一种样品快速提取净化装置,包括离心管、净化抽液管、粉碎涡旋离心一体仪和阻液适配器；粉碎涡旋离心一体仪是集样品粉碎、混合、离心功能为一体的仪器；净化抽液管由净化管、抽提杆、上筛板、下筛板组成,净化管的下端部为抽液口,抽液口上方依次设置下筛板和上筛板,上下筛板之间填充有净化材料,上筛板的上方设置抽提杆,抽提杆密封配合在净化管的内壁；阻液适配器可正置或倒置在离心管内,阻液适配器由一密封垫和伸出于密封垫一面的针头组成,密封垫密封配合在离心管的内壁,密封垫开设供净化管的抽液口配合插置的插口。本案实现了样品粉碎、均质、提取、净化等多个步骤的结合,并可以实现批量化样品处理。(The invention discloses a rapid sample extraction and purification device which comprises a centrifuge tube, a purification liquid suction tube, a crushing vortex centrifugation integrated instrument and a liquid blocking adapter; the crushing, vortex and centrifugation integrated instrument integrates the functions of crushing, mixing and centrifuging a sample; the purification liquid pumping pipe consists of a purification pipe, a pumping rod, an upper sieve plate and a lower sieve plate, wherein the lower end part of the purification pipe is a liquid pumping port, the lower sieve plate and the upper sieve plate are sequentially arranged above the liquid pumping port, a purification material is filled between the upper sieve plate and the lower sieve plate, the pumping rod is arranged above the upper sieve plate, and the pumping rod is in sealing fit with the inner wall of the purification pipe; the liquid blocking adapter can be arranged in the centrifugal tube in an upright or inverted mode, the liquid blocking adapter is composed of a sealing gasket and a needle head extending out of one surface of the sealing gasket, the sealing gasket is matched with the inner wall of the centrifugal tube in a sealing mode, and a socket for matching and inserting a liquid pumping port of the purifying tube is formed in the sealing gasket. The scheme realizes the combination of multiple steps of sample crushing, homogenizing, extracting, purifying and the like, and can realize batch sample treatment.)

1. The utility model provides a purifier is drawed fast to sample which characterized in that: comprises a centrifuge tube, a purification liquid suction tube, a crushing vortex centrifugation integrated instrument and a liquid blocking adapter;

the crushing, vortex and centrifugation integrated instrument integrates the functions of crushing, mixing and centrifuging a sample; the crushing, vortex and centrifugation integrated instrument is provided with a placing opening for placing the centrifugal tube;

the purification liquid pumping pipe consists of a purification pipe, a pumping rod, an upper sieve plate and a lower sieve plate, wherein the lower end part of the purification pipe is a liquid pumping port, the lower sieve plate and the upper sieve plate are sequentially arranged above the liquid pumping port, a purification material is filled between the upper sieve plate and the lower sieve plate, the pumping rod is arranged above the upper sieve plate, and the pumping rod is in sealing fit with the inner wall of the purification pipe;

the liquid blocking adapter can be arranged in the centrifugal tube in an upright or inverted mode, the liquid blocking adapter is composed of a sealing gasket and a needle head extending out of one side of the sealing gasket, the sealing gasket is in sealing fit with the inner wall of the centrifugal tube, and the sealing gasket is provided with a socket for matching and inserting a liquid pumping port of the purifying tube.

2. The rapid sample extraction and purification device as claimed in claim 1, wherein: still include the liquid suction pipe adapter, the liquid suction pipe adapter is width about, narrow and the intercommunication piece of both ends intercommunication from top to bottom, liquid suction pipe adapter upper end supplies the drawing liquid mouth to insert, the size of liquid suction pipe adapter upper end with the drawing liquid mouth size adaptation of purge tube, liquid suction pipe adapter lower extreme is planted in the socket, the size of liquid suction pipe adapter lower extreme with the size adaptation of socket.

3. The rapid sample extraction and purification device as claimed in claim 1, wherein: sealed pad is made by deformable's material, the inner wall of centrifuging tube is equipped with a plurality of expansion position corresponding to the sample volume that will install additional, the internal diameter that expands the position is greater than the internal diameter of centrifuging tube, just the internal diameter that expands the position equals the external diameter of sealed pad.

4. A device for rapidly extracting and purifying a sample as claimed in claim 3, wherein: the number of the expansion positions is three, and the expansion positions correspond to positions of 40mL, 30mL and 20mL respectively.

5. The rapid sample extraction and purification device as claimed in claim 4, wherein: the gasket may be made of rubber.

6. The rapid sample extraction and purification device as claimed in claim 1, wherein: the upper sieve plate and the lower sieve plate are both plates which are formed by sintering and are provided with a plurality of pores.

7. The rapid sample extraction and purification device as claimed in claim 1, wherein: the purifying material can adopt ethylenediamine-N-propyl silane, C18 or graphite.

8. The rapid sample extraction and purification device as claimed in claim 1, wherein: the needle head is funnel-shaped.

Technical Field

The invention relates to the technical field of sample detection instruments, in particular to a rapid sample extraction and purification device.

Background

At present, pesticide or veterinary drug is screened in food and traditional Chinese medicine samples, as the detection targets are more, a QuEChERS mode is often adopted, and a sample test solution for chromatography or chromatography-mass spectrometry combined detection can be obtained by quickly extracting and purifying a sample. Since common test tubes are filled into salt bags and filler bags to carry out the steps in the market, the salt bags and the filler bags need to be subpackaged into corresponding appliances in advance in the actual detection process, but the salt bags and the filler bags are subpackaged into the corresponding appliances in advance, so that heavy repeated work is brought, and the actual detection flux is limited; in addition, in the existing experimental operation process, nonstandard operation still exists between steps, and other modes or instrument assistance is still needed.

Disclosure of Invention

The invention aims to provide a rapid sample extraction and purification device, which realizes the combination of multiple steps of crushing, homogenizing, extracting, purifying and the like and can realize high-throughput and batch sample treatment.

In order to achieve the above purpose, the solution of the invention is: a sample rapid extraction and purification device comprises a centrifuge tube, a purification liquid suction tube, a crushing vortex centrifugation integrated instrument and a liquid blocking adapter;

the crushing, vortex and centrifugation integrated instrument integrates the functions of crushing, mixing and centrifuging a sample; the crushing, vortex and centrifugation integrated instrument is provided with a placing opening for placing the centrifugal tube;

the purification liquid pumping pipe consists of a purification pipe, a pumping rod, an upper sieve plate and a lower sieve plate, wherein the lower end part of the purification pipe is a liquid pumping port, the lower sieve plate and the upper sieve plate are sequentially arranged above the liquid pumping port, a purification material is filled between the upper sieve plate and the lower sieve plate, the pumping rod is arranged above the upper sieve plate, and the pumping rod is in sealing fit with the inner wall of the purification pipe;

the liquid blocking adapter can be arranged in the centrifugal tube in an upright or inverted mode, the liquid blocking adapter is composed of a sealing gasket and a needle head extending out of one side of the sealing gasket, the sealing gasket is in sealing fit with the inner wall of the centrifugal tube, and the sealing gasket is provided with a socket for matching and inserting a liquid pumping port of the purifying tube.

As a further improvement of the above scheme, the purification device further comprises a liquid pumping pipe adapter, wherein the liquid pumping pipe adapter is a communicating piece which is wide at the upper part and narrow at the lower part and is communicated with the upper end and the lower end, a liquid pumping port is inserted into the upper end of the liquid pumping pipe adapter, the size of the upper end of the liquid pumping pipe adapter is matched with that of the liquid pumping port of the purification pipe, the lower end of the liquid pumping pipe adapter is inserted into the socket, and the size of the lower end of the liquid pumping pipe adapter is matched.

As the further improvement of above-mentioned scheme, sealed the pad is made by deformable material, the inner wall of centrifuging tube is equipped with a plurality of inflation position corresponding to the sample volume that will install additional, the internal diameter of inflation position is greater than the internal diameter of centrifuging tube, just the internal diameter of inflation position equals the external diameter of sealed pad.

As a further improvement of the scheme, the number of the expansion positions is three, and the expansion positions correspond to positions of 40mL, 30mL and 20mL respectively.

As a further improvement of the above solution, the sealing gasket may be made of rubber.

As a further improvement of the above scheme, the upper sieve plate and the lower sieve plate are both sintered plate blocks with a plurality of pores.

As a further improvement of the above solution, the needle is funnel-shaped.

After the scheme is adopted, the invention has the beneficial effects that:

the invention can be applied to the inspection of samples such as food, traditional Chinese medicine and the like, is particularly suitable for the detection of residues of traditional Chinese pesticide and veterinary medicine, has strong practicability, can be matched with different crushing homogenization or ultrasonic homogenization extraction modes, and realizes the rapid extraction and purification of the samples by the combination of crushing, homogenization, extraction and purification; and can realize the quick processing of batch sample, need not manual salt package and filler of placing, can also extract alone as required, purify alone or the combination of two kinds of steps, the flexibility is high.

Drawings

FIG. 1 is an assembly view of the overall structure of one embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a pulverizing vortex centrifugation all-in-one instrument according to an embodiment of the present invention;

FIG. 3 is a schematic view of a purge draw tube according to an embodiment of the present invention;

FIG. 4 is a schematic view of a centrifuge tube according to an embodiment of the present invention;

FIG. 5 is a schematic view of a liquid blocking adapter according to an embodiment of the present invention;

fig. 6 is a schematic structural view of a liquid suction tube adapter according to an embodiment of the present invention.

Description of reference numerals:

the device comprises a crushing vortex centrifugation integrated instrument 1, a placing port 11, a purification liquid suction pipe 2, a purification pipe 21, an extraction rod 22, an upper sieve plate 23, a lower sieve plate 24, a liquid suction port 25, a centrifuge tube 3, an expansion position 31, a liquid blocking adapter 4, a sealing gasket 41, a needle 42, a socket 43 and a liquid suction pipe adapter 5.

Detailed Description

The invention is described in detail below with reference to the accompanying drawings and specific embodiments.

The invention provides a sample rapid extraction and purification device, which comprises a crushing vortex centrifugation integrated instrument 1, a purification liquid suction pipe 2, a centrifugal pipe 3, a liquid blocking adapter 4 and a liquid suction pipe adapter 5, as shown in figures 1 to 6;

as shown in fig. 1 and 2, the crushing, vortex and centrifugation integrated instrument 1 integrates the functions of crushing, mixing and centrifuging a sample; the crushing, vortex and centrifugation integrated instrument 1 is provided with a placing opening 11 for placing the centrifuge tube 3; the specification of the centrifuge tube 3 may be 50 mL.

As shown in fig. 1 and 3, the purification liquid suction pipe 2 is composed of a purification pipe 21, an extraction rod 22, an upper sieve plate 23, and a lower sieve plate 24, the lower end of the purification pipe 21 is a liquid suction port 25, the lower sieve plate 24 and the upper sieve plate 23 are sequentially disposed above the liquid suction port 25, a purification material (such as ethylenediamine-N-Propylsilane (PSA), C18, graphite, etc.) is filled between the upper sieve plate 23 and the lower sieve plate 24, the extraction rod 22 is disposed above the upper sieve plate 23, and the extraction rod 22 is hermetically fitted on the inner wall of the purification pipe 21 to vacuumize the purification pipe 21.

As shown in fig. 1 and 5, the liquid blocking adapter 4 can be placed right or inverted in the centrifuge tube 3, the liquid blocking adapter 4 is composed of a sealing gasket 41 and a needle 42 extending out of one surface of the sealing gasket 41, the sealing gasket 41 is hermetically matched with the inner wall of the centrifuge tube 3, and the sealing gasket 41 is provided with a socket 43 for the liquid drawing port 25 of the purifying tube 21 to be inserted in a matching manner.

As shown in fig. 1 and 4, the sealing gasket 41 is made of a deformable material, and in this embodiment, the sealing gasket 41 may be made of rubber; the inner wall of the centrifuge tube 3 is provided with a plurality of expansion positions 31 corresponding to the volume of the sample to be loaded, the number of the expansion positions 31 on the centrifuge tube 3 is three, the expansion positions are respectively corresponding to the positions of 40mL, 30mL and 20mL, the inner diameter of the expansion position 31 is larger than that of the centrifuge tube 3, and the inner diameter of the expansion position 31 is equal to the outer diameter of the sealing gasket 41, when the centrifuge tube 3 needs to be provided with the liquid-blocking adapter 4, the user can press the packing 41 downward, the packing 41 is in a state of being deformed by pressure when it does not reach the expansion site 31, when the gasket 41 reaches the desired expansion position 31, the gasket 41 expands outward to fill the expansion position 31, therefore, the installation of the liquid blocking adapter 4 is completed, of course, the volume needing to be changed is only required to lift or press down the sealing gasket 41 again, and the structure is convenient for users to meet the requirement of quickly adjusting samples with different volumes. Go up sieve 23 and sieve 24 down can inject purifying material in a certain position in the purge tube 21, in this embodiment go up sieve 23 and sieve 24 and be the plate that has a plurality of micropores that form through the sintering, played the effect of dual filtration impurity.

As shown in fig. 1 and 6, in order to improve the connection quality between the purification liquid suction pipe 2 and the gasket 41, the liquid suction pipe adapter 5 is a communicating member having a wide upper end and a narrow lower end, and both the upper end and the lower end are communicated, the upper end of the liquid suction pipe adapter 5 is inserted into the liquid suction port 25, the upper end of the liquid suction pipe adapter 5 is matched with the liquid suction port 25 of the purification pipe 21, the lower end of the liquid suction pipe adapter 5 is inserted into the socket 43, and the lower end of the liquid suction pipe adapter 5 is matched with the socket 43. When in use, the liquid suction pipe adapter 5 with different sizes can be replaced according to the size change of the purifying liquid suction pipe 2 and the sealing gasket 41.

The invention is suitable for detecting pesticide and veterinary drug residues, the used samples comprise vegetable samples, pork samples and the like, and the purification method comprises the following steps:

s1: firstly, coarsely crushing a sample, putting the sample into a centrifuge tube 3 which contains a proper amount of inorganic salt (such as magnesium sulfate, sodium chloride, magnesium chloride and the like) or a combination of the inorganic salt and the magnesium chloride in advance, then putting a proper amount of proton (such as ceramic proton or stainless steel proton) and adding a certain amount of organic extracting solution (such as acetonitrile and the like);

s2: the liquid blocking adapter 4 is rightly arranged in the centrifugal tube 3 and used for preventing the solution from overflowing in the vortex oscillation process, the sealing gasket 41 is located at the required expansion position 31, and at the moment, the rightly arranged position means that the needle 42 extends into the bottom of the centrifugal tube 3 and is a proper distance away from the extracting solution;

s3: placing the centrifugal tube 3 on a crushing, whirling and centrifuging integrated machine for whirling, vibrating and centrifuging;

s4: after centrifugation, the liquid blocking adapter 4 of the centrifuge tube 3 can be inverted, the inversion refers to the outward state of the needle 42, then the centrifuge tube 3 is inverted, and the centrifuged solution is led out firstly, so that independent extraction is realized; or following the purification step, the lower end of the liquid suction pipe adapter 5 is inserted into the socket 43 of the liquid blocking adapter 4, the extraction rod 22 is pulled, the extracting solution at the bottom of the centrifuge tube 3 enters the purified liquid suction pipe 2 through the needle 42 of the liquid blocking adapter 4 of the centrifuge tube 3, the liquid suction pipe adapter 5 and the liquid suction port 25 of the purified liquid suction pipe 2, and the extracting solution can be used for testing the sample and can also be independently purified.

The solution is unlikely to flow out of the needle 42 in general, but for the sake of safety, the present embodiment designs the needle 42 in a funnel shape, and the solution flows back along the slope even if the solution partially flows out.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the design of the present invention, and all equivalent changes made in the design key point of the present invention fall within the protection scope of the present invention.