阅读说明：本技术 川续断皂苷乙在制备防治血管疾病药物中的应用 (Application of dipsacus asperoides saponin B in preparation of medicine for preventing and treating vascular diseases ) 是由 庹勤慧 霍艳杰 廖端芳 于 2020-12-09 设计创作，主要内容包括：本发明公开了川续断皂苷川续断皂苷乙在制备预防或治疗血管疾病药物中的用途,为川续断皂苷的应用提供了新的范围,同时本发明还提供了一种用于防治血管疾病的治疗药物,本发明人通过研究发现川续断皂苷乙参与细胞的增殖和血管重构过程,具有防治血管损伤后内膜增生作用,故本发明药物尤其适用于防治血管内膜增生类的血管疾病。(The invention discloses an application of dipsacus asperoides saponin B in preparing a medicament for preventing or treating vascular diseases, provides a new range for the application of dipsacus asperoides saponin, and simultaneously provides a medicament for preventing and treating vascular diseases.)

1. Application of dipsacus asperoides B in preparing medicine for preventing and treating vascular diseases is provided.

2. Use according to claim 1, characterized in that the vascular disease is a vascular disease associated with intimal hyperplasia of the blood vessels.

3. Use according to claim 1 or 2, characterized in that the medicament is a medicament for the prevention and treatment of intimal hyperplasia in blood vessels.

4. Use according to claim 1 or 2, characterized in that the medicament further comprises a pharmaceutically acceptable carrier or adjuvant.

5. Use according to claim 4, characterized in that the pharmaceutically acceptable carrier or adjuvant comprises one or more solid, semi-solid or liquid adjuvants.

6. Use according to claim 1 or 2, characterized in that the medicament is a pharmaceutically acceptable formulation.

7. Use according to claim 6, characterized in that the pharmaceutically acceptable formulation comprises tablets, capsules, injections, granules, pills, syrups, powders or ointments.

8. A medicine for preventing and treating vascular diseases comprises asperosaponin B or its pharmaceutically acceptable salt or ester.

Technical Field

The invention belongs to the field of medicines, and particularly relates to application of dipsacus asperoides B in preparation of a medicine for preventing or treating vascular diseases.

Background

Vascular remodeling is the common pathological basis for a variety of diseases such as atherosclerosis, angioplasty, hypertension, and the like. Research shows that the formation of blood vessel reconstruction is a series of structural and functional abnormalities caused by the change of the internal and external environments of blood vessels, including the proliferation, migration, apoptosis, change of mechanism components and the like of cells, and on the basis of the series of dynamic changes, the stimulation of the blood vessels to the internal and external environments of the blood vessels generates structural changes, such as the thickening of the vessel walls, the increase of the ratio of the vessel walls to the vessel lumens and the reduction of the number of tiny arteries, thereby immediately causing the functional abnormalities of the blood vessels. Among them, proliferation and migration of smooth muscle cells (VSMCs) are among the most prominent pathological processes. At present, neointimal hyperplasia and vascular remodeling are the main reasons for clinical vascular restenosis and lumen treatment failure, so how to prevent and treat the neointimal hyperplasia is an urgent problem to be solved in the industry.

Dipsacus asperoides saponin B is derived from dipsacus asperoides and lonicera confusa and is one of the main components of lonicera confusa; the dipsacus asperoides saponin B is pentacyclic triterpene oleanane type saponin, and pentacyclic triterpene saponin compounds are novel in chemical structure and complex in stereo structure. The compound has various biological activities of protecting liver, resisting inflammation, resisting tumor, regulating immunity and the like. Studies report that dipsacus asperoides saponin B protects acetaminophen-induced liver damage. However, reports on the treatment effect of the traditional Chinese medicine on the intima hyperplasia vascular remodeling are not found so far.

The pharmacological action of the dipsacoside B on the cardiovascular system is a subject which has been concerned by the inventor for a long time, and in the previous research, the inventor finds that: the dipsacus asperoides saponin B has inhibitory effect on angiotensin II induced vascular smooth muscle cell proliferation. However, the action mechanism of the teasel saponin B has no report at present if the teasel saponin B has an inhibitory effect on vascular remodeling. The medicine has extremely high potential value and social significance when being developed into the medicine for treating diseases such as pulmonary hypertension and the like.

Disclosure of Invention

The object of the present invention is to solve at least one of the technical problems of the prior art. Therefore, the invention provides a new application of dipsacoside B in pharmacy, in particular to an application of the dipsacoside B in preparing a medicament for preventing or treating vascular diseases.

The chemical structural formula of the teasel saponin B is shown as follows (molecular formula: C)₅₃H₈₆O₂₂Molecular weight: 1075.44),

the inventor adopts an SD rat abdominal aorta balloon injury model in research, and detects the influence of the teasel saponin B on the intimal hyperplasia thickness by an HE staining method; the result shows that the asperosaponin B obviously reduces the intimal hyperplasia thickness; the cell proliferation model of Ang-II induced rat Vascular Smooth Muscle Cells (VSMCs) is established in vitro, the influence of the dipsacoside on the cell proliferation activity is detected, and the Edu method is used for determining the change of the cell proliferation level when the compound is used, and the result shows that the dipsacoside can obviously inhibit the cell proliferation; the cell cycle is measured by a flow method, and the asperosaponin B can block the synthesis of VSMCs from the G1 stage to the S stage, thereby influencing the cell proliferation. The dipsacoside B can inhibit cell proliferation and down-regulate TOP2a, p-AKT/AKT and PCNA expression; experiments prove that the compound dipsacus asperoides saponin B can inhibit the proliferation process of vascular smooth muscle cells and has the effects of preventing and treating vascular intimal hyperplasia.

Namely the application of the compound dipsacoside B in the preparation of the medicine for preventing and treating vascular diseases.

Further, the vascular disease is vascular disease associated with intimal hyperplasia of blood vessels.

From the experimental results, pharmacological experiments and in vitro and in vivo experiments in the embodiment of the invention show that the dipsacus asperoides saponin B participate in the processes of cell proliferation and vascular remodeling, have the effect of preventing and treating intimal hyperplasia after vascular injury, and can be further used for preparing the medicine for preventing and treating the intimal hyperplasia of blood vessels. The medicine comprises the teasel saponin B or pharmaceutically acceptable salt or ester thereof.

Therefore, the invention also aims to provide a medicament prepared from the dipsacoside for preventing and treating the vascular intimal hyperplasia.

According to some embodiments of the invention, the medicament further comprises a pharmaceutically acceptable carrier or excipient.

Further, the pharmaceutically acceptable carrier or excipient comprises one or more solid, semi-solid or liquid excipients.

According to some embodiments of the invention, the medicament is a pharmaceutically acceptable formulation.

Further, the pharmaceutically acceptable preparation includes tablets, capsules, injections, granules, pills, syrups, powders or ointments.

The present invention is further illustrated by way of examples and is not therefore to be limited in scope by the examples described.

Drawings

Fig. 1 is a positional diagram of the docking of each target protein and teasel saponin B molecule in example 1 of the present invention, wherein; in fig. 1, a is a docking diagram of a target protein TOP2a and dipsacus asperoides saponin B, B is a docking diagram of a target protein BCL-xL and dipsacus asperoides saponin B, C is a docking diagram of a target protein PYGM and dipsacus asperoides saponin B, and D is a docking diagram of a target protein PTGS1 and dipsacus asperoides saponin B.

FIG. 2 is a graph showing the effect of asperosaponin B on VSMCs proliferation activity in example 2 of the present invention

Fig. 3 is a graph showing the effect of asperosaponin B on Ang-II-induced VSMCs proliferation in example 2 of the present invention, where a in fig. 3 is an influence image and B is an analysis result of the image.

FIG. 4 is a graph showing the expression of the saponins of Dipsacus asperoides B on TOP2a, p-AKT/AKT, PCNA proteins in example 3 of the present invention and the analysis results.

FIG. 5 is a representation of the vascular intima HE staining profile of rats in each group in example 4 of the present invention

FIG. 6 example 4 of the present invention shows the effect of Dipsacasperoides saponin B on neointima

FIG. 7 shows the expression of Dipsacaceae saponin B on alpha-SMA and OPN proteins in example 4

FIG. 8 shows the effect of Dipsacasperoides saponin B on VSMCs cell cycle in example 5 of the present invention

Detailed Description

In order to explain technical contents, achieved objects, and effects of the present invention in detail, the following description is made with reference to the accompanying drawings in combination with the embodiments.

Example 1 prediction of effects of Dipsacasperoiside B on DNA topoisomerase 2a (TOP2a) by Neuropharmacology

The teasel root saponin B used in the embodiment of the invention is purchased from Nanjing spring and autumn biology Limited company, and the purity is more than 98%. Downloading the structural formula of the teasel saponin B by a pubchem database, predicting the target of the teasel saponin B on a ChemMapper database according to the condition that the similarity score is greater than 0.8 and the BindingDB biological activity library, and downloading the predicted target. And (3) docking the teasel saponin B with a target by using IGEMDOCK molecular docking software, firstly selecting an active docking site, inputting a compound for docking, analyzing and scoring to determine a subsequent research object. The database prediction results are shown in table 1 and fig. 1.

TABLE 1 prediction of Dipsacus asperoides saponin B targets by ChemMapper database

The results show that: the values of TOP2a, BCL-xL, PYGM and PTGS1 in the iGEMDOCK docking are-84.7 kJ. mol-1, -70.9 kJ. mol-1, -63.6 kJ. mol-1 and-79.5 kJ. mol-1 respectively. The lower the energy value, the more stable the docking, wherein the docking energy value of the teasel saponin B and TOP2a is the lowest, the effect is the best, so the Top IIalpha is selected as the subsequent research target.

Example 2 experiment of inhibitory Activity of Dipsacasperosaponin B on Ang-II-induced proliferation level of VSMCs

The cell activity was measured by MTT method, and VSMCs in logarithmic growth phase were plated at a density of 8000 cells/well. Meanwhile, equal volume of PBS is added into the peripheral hole, so that the marginalization effect is avoided. After 24h, dissolving the teasel root saponin B by using a culture medium, wherein the gradient is as follows: 1. 3, 10, 30, 100, 300 and 1000 mu M, each empty 100 mu L, incubating for 2 hours, discarding, adding Angiotensin II (Angiotensin II, Ang-II) (1 mu M) to induce proliferation model, incubating for 24 hours, discarding, adding 100 mu L of MTT solution to each well, and continuing incubating for 4 hours. The culture medium was then aspirated, 150. mu.L DMSO was added, the shaker was shaken in the dark for 10min, and the OD was measured at a wavelength of 570nm on a microplate reader. The results are shown in FIG. 2.

The results show that: teasel saponin b had no significant effect on VSMCs viability (P >0.05) at concentrations ranging from 1 to 1000 μ M compared to the control group, i.e. there was no cytotoxicity of teasel saponin b on VSMCs at this concentration range (fig. 2A). The influence of the asperosaponin B on the proliferation of the Ang-II-induced VSMCs compared with a control group, the model group can promote the proliferation of VSMCs cells (P < 0.05). Compared with the model group, the teasel saponin B with the concentration of 3 to 1000 mu M has obvious inhibition effect on the proliferation activity of the VSMCs (figure 2B).

Cell proliferation was detected by EdU method. Cells in the logarithmic growth phase were seeded at 1X 104 cells per well in a 96-well plate and cultured to the normal growth phase. Cells were treated in groups for 24 hours: control group (high sugar culture medium), model group (Ang-II, 1 μ M), positive control group Curcumin (Curcumin,5 μ M) + Ang-II, and Dipsacasperoides B (3, 10, 30 μ M) + Ang-II. Cells were labeled with 50. mu.M EdU added at 100. mu.L/well for 4 hours. The culture medium was removed and 100. mu.L/well of 4% paraformaldehyde cells were fixed for 15 minutes. The fixative is removed and the cells are washed 3 times with 1ml of wash solution per well, 3-5 minutes each. The wash solution was removed and 100. mu.L of 0.3% Triton X-100 was added to each well and incubated for 10-15 minutes. The permeate was removed and the wells were washed 1-2 times with 100. mu.L of wash solution, 3-5 minutes each time. Add 100. mu.L of LClick reaction per well and gently shake the plate. Incubate for 30 minutes at room temperature in the dark. The Click reaction solution was aspirated and washed 3 times with washing solution for 3 to 5 minutes each. After the washing solution was aspirated, 100. mu.L of Hoechst 33342 solution was added to each well, and the mixture was incubated for 10 minutes at room temperature in the dark. The Hoechst 33342 solution was aspirated. Washing with washing solution for 3 times, each for 3-5 min. That is, fluorescence detection was performed, and an image was taken by an inverted fluorescence microscope (Olympus) and the analysis result was shown in fig. 3.

The experimental results show that the model group can promote the proliferation of VSMCs compared with the control group (P < 0.01). Compared with the model group, the teasel saponin B with the concentration of 3, 10 and 30 mu M has obvious inhibition effect on the proliferation of VSMCs, and the teasel saponin B can inhibit the proliferation activity of the cells of the VSMCs induced by Ang-II.

Example 3 inhibition of Ang-II-induced expression of VSMCs cell proliferation-related proteins by Dipsacasperosaponin B

Cells were seeded at 5X105/ml in petri dishes and treated for 24 hours in the following corresponding groups after the degree of cell confluence reached 80%. The specific grouping is as follows: control group (high sugar culture medium), model of Ang-II (1 μmol/L) treatment group, positive control of Curcumin (Curcumin, Cur) treatment group (Cur,5 μmol/L) + Ang-II, and Dipsacus asperoides saponin B (3, 10, 30 μmol/L) + Ang-II. Cell protein is collected by cracking, and SDS-PAGE gel is used for preparing a kit to prepare 10% SDS-polyacrylamide gel; the loading well was loaded with 20 ul. Electrophoresis is carried out at 100V for 100 minutes, and the gel is placed on a PVDF membrane and placed on a wet-transfer membrane, the current is 300mA, and the time is 2 hours. PVDF is put into TBS-T containing 5 percent of skimmed milk powder and is kept at the normal temperature for 45 minutes; TBS-T washing for 5min × 3 times, incubating the primary antibody membrane and primary antibody at a dilution ratio of 1:2000, and incubating at 4 deg.C for 12 hr; washing with TBS-T for 10min × 3 times; incubating the secondary antibody Goat-anti-rabbitIgG (H + L) (ratio of 1: 5000) for 1 hour at room temperature; washing with TBS-T for 10min × 3 times; uniformly dripping a developing solution on the PVDF film, exposing for 30 seconds, and developing and fixing; density analysis was performed in Alpha Imager2200 Gray Scan software. The image and analysis results are shown in FIG. 4.

The model group promoted proliferation of VSMCs cells compared to the control group (P < 0.01). Compared with the model group, the teasel saponin B with the concentration of 3, 10 and 30 mu M has obvious inhibition effect on the proliferation of VSMCs.

The experimental results show that: the asperosaponin B can regulate the expression of TOP2a, p-AKT/AKT and PCNA in VSMCs.

Example 4 the Himalayan teasel saponin B can reduce the intimal hyperplasia thickness of blood vessels and inhibit the expression of cell proliferation-type related proteins in blood vessel tissues

After SD rats are weighed, 3ml/kg of 10% chloral hydrate is anesthetized by intraperitoneal injection, fixed on a simple operating table, and sequentially prepared into skin, disinfected and paved around an operation opening; cutting skin 3cm longitudinally along the middle of neck and 0.2cm to the left, separating latissimus cervicales and left sternocleidomastoid muscle, and searching left common carotid artery along the left side of trachea; separating left common carotid artery with length of about 2.5cm, ligating the bifurcation part of the left external carotid artery and the internal carotid artery with # 1 silk thread, and blocking the blood flow of the proximal common carotid artery with a vascular clamp; puncturing a common carotid artery at a position close to ligation by using a 5ml syringe needle, withdrawing the syringe needle, cutting off a left common carotid artery by using an ophthalmologic scissors longitudinal line for 0.3cm, inserting a 2.0mm multiplied by 10mm balloon catheter (with a 0.014mm guide wire) to the tail end of an abdominal aorta through a broken opening in an antegrade manner, filling the balloon with the pressure of 8kPa while maintaining the pressure, slowly pulling the balloon to the cut (about 15 seconds), pumping to a negative pressure, feeding the balloon again, and repeating the operation for 3 times in total; after the saccule is completely withdrawn, the hemostatic clamp is clamped and closed, the proximal segment of the common carotid artery is ligated by silk thread, the operation port is flushed by gentamycin solution, the subcutaneous tissue and the skin are sutured intermittently, and the penicillin is injected by 10 million U/day muscle after the operation for 3 days, thereby preventing infection. Taking a blood vessel tissue with the length of about O.5cm below a self-ligation site on the common carotid artery, and taking a blood vessel with the same position and length in a sham operation group; perfusing the dipsacoside for 14 days, fixing the aorta with 4% paraformaldehyde, and embedding to obtain paraffin section. HE staining observed intimal hyperplasia thickness. The HE staining pattern of the animal experimental section is shown in FIG. 5, and the analysis results are shown in FIGS. 6 and 7.

The experimental results show that: compared with the sham group, the intima and the blood vessel of the injury group are obviously thickened (P <0.01), and compared with the injury group, the asperosaponin B group can inhibit the formation of the neointima (P < 0.01). Therefore, the dipsacus asperoides saponin B can inhibit the vascular intimal hyperplasia thickness, promote the expression of alpha-SMA and inhibit the expression of OPN.

Example 5 flow method for determining the Effect of Dipsacasperoides saponin B on VSMCs cell cycle

VSMCs were seeded at 1x l05 density in six-well plates and after 80% cell confluence was achieved, media containing 0.3% FBS was synchronized for 24 h. Then the medicine grouping process is carried out for 24 h: control group (high sugar culture medium), model group (Ang-II, 1. mu.M), Dipsacasperoides B (3, 10, 30. mu.M) + Ang-II. Precooling absolute ethyl alcohol at-20 ℃, preparing 75% ethanol fixing solution by using precooled PBS, subpackaging the fixing solution in 2ml of EP tube, and placing the tube on ice for later use. The cells were emptied of medium and washed three times with pre-cooled PBS. Cells were digested with 0.25% pancreatin. The medium containing 10% FBS was added to a six-well plate, and the cells were aspirated to collect a cell suspension. 900rpm/min, centrifuge for 5min, discard the supernatant, add 2ml of precooled PBS to resuspend the cells, centrifuge again for 5min at 900rpm/min, discard the supernatant. And (3) blowing and beating the cells into suspension by using PBS (phosphate buffer solution) remained in a centrifuge tube, then adding the suspension into precooled 75% ethanol fixing solution, and uniformly blowing and beating. The EP tube was sealed with a sealing film and fixed in a refrigerator at 4 ℃ for 24 hours. And sending out for PI staining detection. The results are shown in FIG. 8 and Table 2.

TABLE 2 comparison of the Effect of Dipsacasperoiside B on VSMCs cell cycle

Comparison with control group: p <0.05, compared to model group: # P <0.05

Compared with the control group, the VSMCs in the model group have obviously reduced G1 phase cells and obviously increased S phase cells (P < 0.05). Compared with the model group, the G1 phase cells of the teasel saponin B are obviously increased (P is less than 0.05) and the S phase cells are obviously decreased (P is less than 0.05) in different concentration groups (3, 10 and 30 mu M), and the results show that the teasel saponin B can block the synthesis of VSMCs from the G1 phase to the S phase and further influence the cell proliferation.