阅读说明：本技术 一种获得单细胞mRNA序列的方法 (Method for obtaining single-cell mRNA sequence ) 是由 范飞 程小芳 章文蔚 汪为茂 崔路漫 王欧 于 2018-12-27 设计创作，主要内容包括：本发明公开了一种获得单细胞mRNA序列的方法。本发明的方法：(1)利用细胞标签载体捕获细胞的mRNA,反转录得到具有细胞标签的cDNA；来自于同一个细胞的cDNA具有相同的细胞标签,来自于不同细胞的cDNA具有不同的细胞标签；(2)利用转座酶复合体和分子标签载体,得到具有分子标签的多个cDNA片段；来自于同一cDNA的各个片段具有相同的分子标签,来自于不同cDNA的片段具有不同的分子标签；(3)高通量测序；(4)根据分子标签进行序列拼接,得到每个mRNA的序列；根据细胞标签,得到每个单细胞的所有mRNA的序列。本发明提供的方法可用于高通量获得大量单细胞中每个单细胞的所有mRNA的序列。(The invention discloses a method for obtaining a single-cell mRNA sequence. The method comprises the following steps: (1) capturing mRNA of cells by using a cell tag carrier, and performing reverse transcription to obtain cDNA with a cell tag; cDNAs from the same cell have the same cell tag, and cDNAs from different cells have different cell tags; (2) utilizing the transposase complex and the molecular tag carrier to obtain a plurality of cDNA fragments with molecular tags; each fragment from the same cDNA has the same molecular tag, and fragments from different cdnas have different molecular tags; (3) high-throughput sequencing; (4) performing sequence splicing according to the molecular tags to obtain a sequence of each mRNA; from the cell tags, the sequences of all mrnas were obtained for each single cell. The method provided by the invention can be used for obtaining all mRNA sequences of each single cell in a large number of single cells at high flux.)