阅读说明：本技术 一种猪苓薄层鉴别方法 (Thin layer identification method for polyporus umbellatus ) 是由 齐晓丹 刘海滨 张淹 孔令梅 周祥山 李士栋 王春艳 孙阳恩 于 2020-05-14 设计创作，主要内容包括：本发明公开一种猪苓的薄层鉴别方法,包括步骤如下：(1)制备猪苓供试品溶液；(2)在硅胶薄层板上,以环己烷-乙酸乙酯-甲酸,体积比为8:1:0.02～0.2为展开剂展开,(3)直接置紫外灯下检视,或喷以硫酸乙醇,晾干,置紫外灯下检视。本发明操作简单,快速,能展示多个猪苓特征薄层斑点；提供一种以猪苓药材为对照的薄层鉴别方法,填补《中国药典》中无猪苓对照药材的空白；同时该方法可应用于猪苓药材、饮片、猪苓汤的定性鉴别,对猪苓中成分在药材、猪苓水提物中的相关性研究具有重要意义。(The invention discloses a thin-layer identification method of polyporus umbellatus, which comprises the following steps: (1) preparing a polyporus umbellatus test solution; (2) spreading on a silica gel thin layer plate by using a developing agent of cyclohexane-ethyl acetate-formic acid with the volume ratio of 8:1: 0.02-0.2, and (3) directly viewing under an ultraviolet lamp, or spraying sulfuric acid ethanol, drying in the air, and viewing under the ultraviolet lamp. The method is simple and quick to operate, and can display a plurality of characteristic thin layer spots of the polyporus umbellatus; provides a thin-layer identification method taking grifola as a reference, fills the blank that no grifola reference medicinal material exists in Chinese pharmacopoeia; meanwhile, the method can be applied to qualitative identification of the polyporus umbellatus medicinal materials, decoction pieces and the polyporus umbellatus soup, and has important significance for research on correlation of components in the polyporus umbellatus in the medicinal materials and the polyporus umbellatus aqueous extract.)

1. A thin layer identification method of polyporus umbellatus is characterized by comprising the following steps:

(1) preparing a polyporus umbellatus test solution;

(2) spreading on a silica gel thin layer plate by using cyclohexane-ethyl acetate-formic acid as a developing agent in a volume ratio of 8:1: 0.02-0.2;

(3) inspecting under ultraviolet lamp, or spraying ethanol sulfate, air drying, and inspecting under ultraviolet lamp.

2. The thin layer chromatography of polyporus umbellatus as claimed in claim 1, wherein the volume ratio of ethyl acetate to formic acid is 8:1: 0.1.

3. The thin layer chromatography of Polyporus umbellatus as claimed in claim 1, wherein the detection wavelength is 365 nm; the volume percentage of the sulfuric acid ethanol is 10%.

4. The thin layer chromatography of umbellate pore fungus according to claim 1, for use in a composition comprising umbellate pore fungus.

5. The thin-layer chromatography of umbellate pore fungus as claimed in claim 1, wherein said thin-layer chromatography is used for umbellate pore fungus herbs or umbellate pore fungus soup.

6. The thin layer chromatography of Polyporus umbellatus as claimed in claim 1, wherein the detection wavelength is 365 nm.

Technical Field

The invention belongs to the technical field of traditional Chinese medicine quality detection, and relates to a thin layer identification method for polyporus umbellatus.

Background

Polyporus umbellatus is dried sclerotium of Polyporus umbellatus (Pers.) Fries of Polyporaceae. Has effects in promoting diuresis and eliminating dampness, and can be used for treating dysuria, edema, diarrhea, stranguria with turbid urine, and leukorrhagia. At present, the standard of the polyporus umbellatus only comprises the thin-layer identification of ergosterol, but the ergosterol has poor water solubility, is difficult to detect in a polyporus umbellatus water extract, and cannot qualitatively investigate the transferability of polyporus umbellatus components in medicinal materials, decoction pieces and the water extract. The research provides a polyporus umbellatus thin-layer identification method, and polyporus umbellatus medicinal materials are used as a reference, so that a plurality of polyporus umbellatus thin-layer spots can be displayed, polyporus umbellatus medicinal materials, decoction pieces and polyporus umbellatus components in an aqueous extract can be simultaneously detected, and the correlation of qualitative indexes of the polyporus umbellatus medicinal materials, namely the polyporus umbellatus aqueous extract can be conveniently researched.

Disclosure of Invention

The invention provides a thin-layer polyporus umbellatus identification method, which can not only show a plurality of characteristic spots of polyporus umbellatus in a thin-layer chromatogram, but also can simultaneously detect polyporus umbellatus components in crude drug slices of polyporus umbellatus and water extracts, and is convenient for inspecting the transferability of qualitative components of polyporus umbellatus in crude drug slices and water decoction extracts containing polyporus umbellatus formulas.

In order to achieve the purpose, the invention adopts the following technical scheme.

A thin layer identification method of polyporus umbellatus comprises the following steps:

(1) preparing a polyporus umbellatus test solution;

(2) developing on a silica gel thin layer plate by using cyclohexane-ethyl acetate-formic acid as a developing agent in a volume ratio of 8:1: 0.02-0.2, wherein formic acid is a developing system regulator;

(3) inspecting under ultraviolet lamp, or spraying ethanol sulfate, air drying, and inspecting under ultraviolet lamp.

Preferably, the volume ratio of the cyclohexane-ethyl acetate-formic acid is 8:1: 0.1.

Further, the detection wavelength is 365 nm; the volume percentage of the sulfuric acid ethanol is 10%.

The thin layer identification method of Polyporus umbellatus is used for composition containing Polyporus umbellatus.

The thin-layer identification method of the polyporus umbellatus is used for a polyporus umbellatus medicinal material or a polyporus umbellatus soup.

The innovation points of the invention are as follows:

1. the thin-layer identification method of the polyporus umbellatus is established, the operation is simple and rapid, and compared with the thin-layer identification method of the polyporus umbellatus in pharmacopoeia, the method can display a plurality of characteristic spots in the polyporus umbellatus and reflect more thin-layer information of the polyporus umbellatus.

2. Fills the blank that no polyporus umbellatus reference medicinal material exists in Chinese pharmacopoeia, and provides a thin-layer identification method directly taking polyporus umbellatus medicinal material as reference.

3. The method can be simultaneously applied to qualitative identification of the grifola components in the grifola medicinal material decoction pieces and the grifola decoction, and can be applied to investigation of correlation of the grifola medicinal material and the grifola decoction qualitative components.

Drawings

FIG. 1 is a chromatogram of a systematic optimization investigation experiment developed by thin-layer grifola identification method; a is cyclohexane-ethyl acetate-formic acid (4:1: 0.1); b is cyclohexane-ethyl acetate-formic acid (8:1: 0.1); 1. 5: polyporus decoction pieces (from Beijing Qiancao herbal decoction pieces Co., Ltd.); 2: produced in Fuping county, Hanzhong city, Shaanxi province; 3: produced in Koelreuteria paniculata of south-south Henan province; 4: produced in Xixia county of south-yang city of Henan province;

FIG. 2 is a chromatogram of a formic acid dosage investigation test of a spreading system of a thin-layer grifola identification method; a is cyclohexane-ethyl acetate-formic acid (8:1: 0.02); b is cyclohexane-ethyl acetate-formic acid (8:1: 0.1); c, cyclohexane-ethyl acetate-formic acid (8:1: 0.2); 1. 5: polyporus decoction pieces (from Beijing Qiancao herbal decoction pieces Co., Ltd.); 2: produced in Fuping county, Hanzhong city, Shaanxi province; 3: produced in Koelreuteria paniculata of south-south Henan province; 4: produced in Xixia county of south-yang city of Henan province;

FIG. 3 is a chromatogram of inspection of extractant and comparison with pharmacopoeia method for thin-layer identification of Polyporus umbellatus; a: a pharmacopoeia method; b: the method of the invention; 1: ergosterol (111845-201604); 2: polyporus umbellatus (methanol extraction) 3: polyporus umbellatus (ether extraction);

FIG. 4 is a chromatogram of a color development mode investigation test of a thin-layer grifola identification method; a, placing the film under 365nm for inspection; b: spraying 10% sulfuric acid ethanol, and inspecting at 365 nm; 1. 5: polyporus umbellatus decoction pieces (purchased from Beijing Qiancao Chinese medicinal decoction pieces Co., Ltd.); 2: produced in Fuping county, Hanzhong city, Shaanxi province; 3: produced in Koelreuteria paniculata of south-south Henan province; 4: produced in Xixia county of south-yang city of Henan province;

FIG. 5 is a thin-layer chromatogram for identifying Polyporus decoction; a: inspecting at 365 nm; b: spraying 10% sulfuric acid ethanol, and inspecting at 365 nm; 1: polyporus (from Beijing Qiancao herbal pieces of Chinese medicine Co., Ltd.); 2: 1, grifola soup; 3: polyporus umbellatus soup 2; 4: polyporus umbellatus soup 3; 5: lacking Polyporus umbellatus and Polyporus umbellatus soup.

Detailed Description

The following examples are further illustrative of the present invention, but the present invention is not limited thereto.