阅读说明：本技术 一种水媒法制备高蛋白质含量亚麻籽蛋白的方法 (Method for preparing high-protein-content linseed protein by aqueous medium method ) 是由 华欲飞 孙旦 张彩猛 孔祥珍 陈业明 李兴飞 于 2020-05-29 设计创作，主要内容包括：本发明公开了一种水媒法制备高蛋白质含量亚麻籽蛋白的方法,包括,将亚麻籽原料加水进行湿法磨浆,将湿法磨浆后产物的pH调节至7.0～10.0,搅拌提取,过滤除渣,得亚麻籽浆液；将所述亚麻籽浆液第一次离心分离,得到第一轻相、中间相和第一重相；将所述中间相的pH调节至5.5～8.5,盐浓度调节至0～0.1mol/L,在温度为4～20℃下第二次离心分离,得到第二轻相和第二重相；将第二重相的pH调节至7.0～7.5之后喷雾干燥,得到亚麻籽浓缩蛋白；将第二轻相的pH调节至4.0～5.0,第三次离心分离,得到凝乳；将所述凝乳的pH调节至7.0～7.5之后喷雾干燥,得到亚麻籽分离蛋白。本发明不引入酶制剂与有机溶剂,通过条件优化,能够有效去除蛋白液中残留的脂质。(The invention discloses a method for preparing high-protein-content linseed protein by a water medium method, which comprises the steps of adding water into a linseed raw material for wet grinding, adjusting the pH value of a product obtained after the wet grinding to 7.0-10.0, stirring, extracting, filtering and removing slag to obtain linseed pulp; centrifuging the flaxseed slurry a first time to obtain a first light phase, an intermediate phase and a first heavy phase; adjusting the pH value of the intermediate phase to 5.5-8.5, adjusting the salt concentration to 0-0.1 mol/L, and performing second centrifugal separation at the temperature of 4-20 ℃ to obtain a second light phase and a second heavy phase; adjusting the pH value of the second heavy phase to 7.0-7.5, and then carrying out spray drying to obtain linseed protein concentrate; adjusting the pH value of the second light phase to 4.0-5.0, and performing centrifugal separation for the third time to obtain curd; and adjusting the pH value of the curd to 7.0-7.5, and then carrying out spray drying to obtain the linseed protein isolate. The method does not introduce an enzyme preparation and an organic solvent, and can effectively remove the residual lipid in the protein solution through condition optimization.)

1. A method for preparing high-protein-content linseed protein by a water medium method is characterized by comprising the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

adding water into a flaxseed raw material to carry out wet grinding, adjusting the pH of a product obtained after the wet grinding to 7.0-10.0, stirring and extracting, and filtering to remove residues to obtain flaxseed pulp;

centrifuging the flaxseed slurry a first time to obtain a first light phase, an intermediate phase and a first heavy phase;

adjusting the pH value of the intermediate phase to 5.5-8.5, adjusting the salt concentration to 0-0.1 mol/L, and performing second centrifugal separation at the temperature of 4-20 ℃ to obtain a second light phase and a second heavy phase;

adjusting the pH value of the second heavy phase to 7.0-7.5, and then carrying out spray drying to obtain linseed protein concentrate;

adjusting the pH value of the second light phase to 4.0-5.0, and performing centrifugal separation for the third time to obtain curd; and adjusting the pH value of the curd to 7.0-7.5, and then carrying out spray drying to obtain the linseed protein isolate.

2. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: the flaxseed raw material is full-fat dehulled flaxseed or cold-pressed dehulled flaxseed meal.

3. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: and adding water for wet pulping, wherein the water adding amount is 0.5-5 times of the weight of the raw materials.

4. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: the stirring extraction time is 0.5-2 h.

5. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: filtering to remove residues, washing the filtered residues with water, filtering again, repeating for 1-3 times, and combining the obtained filtrates to obtain the linseed pulp;

wherein the total amount of the added water is 10-30 times of the weight of the raw materials.

6. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: and (3) performing the first centrifugal separation, namely separating by using a three-phase disc centrifuge under 1000-5000 g.

7. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: and performing the second centrifugal separation, namely separating by using a two-phase disc centrifuge at 3000-12000 g.

8. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: and performing third centrifugal separation, namely separating by using a horizontal spiral centrifuge at 1500-3000 g.

9. The aqueous process of claim 1 for the preparation of high protein content flax seed protein, wherein: and further comprising the steps of adjusting the pH value of the first light phase to 5.0-7.0, freezing, thawing, and centrifuging for the fourth time to obtain the linseed oil.

10. The aqueous process of claim 9 for the preparation of high protein content flax seed protein, wherein: the freezing treatment is carried out for 5-18 h at the temperature of-30 to-5 ℃;

the unfreezing treatment is to unfreeze at the ambient temperature of 10-50 ℃;

and performing centrifugal separation for the fourth time, namely separating by using a three-phase disc centrifuge at 3000-10000 g.

Technical Field

The invention belongs to the technical field of plant protein processing, and particularly relates to a method for preparing high-protein-content linseed protein by a water medium method.

Background

The flaxseed contains 35-45% of oil and 20-30% of protein. The linseed oil is rich in alpha-linolenic acid (50-60 percent), has the unsaturated fatty acid content of about 90 percent, and is high-quality high-end edible oil. Flax seed protein has high nutritive value and is an excellent source of arginine, glutamine, histidine, cysteine and methionine. And has good processing characteristics, and can be used as an ingredient to improve the functional value of food. The high-purity flaxseed protein has a great application value in functional health products. In addition, the residual amount of the oil in the high-purity flaxseed protein is low, the problems of oxidation, deterioration and the like of unsaturated fatty acid can be effectively solved, the quality guarantee period of the product is prolonged, and the utilization value of the protein product is improved.

Aqueous linseed processing is becoming increasingly attractive due to its green concept. The traditional aqueous medium method is used for simultaneously preparing flaxseed oil and protein from full-fat flaxseed, the separation efficiency of the protein and the lipid is far lower than that of a solvent leaching process due to the interaction between the protein and the lipid, the protein part is usually recycled as a byproduct, the protein content of a product is low, the protein yield is about 60-70%, and the protein content is about 65-80%. At present, the preparation of the linseed protein usually comprises the step of separating lipid by a solvent leaching process to improve the protein content of the product, wherein the protein yield is about 60-70%, the content can reach 85-90%, but the problem of organic solvent residue exists, and the preparation does not accord with the green processing concept.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made keeping in mind the above and/or other problems occurring in the prior art.

Therefore, the present invention aims to overcome the defects in the prior art and provide a method for preparing high-protein-content linseed protein by an aqueous medium method, wherein an enzyme preparation and an organic solvent are not introduced, and the condition is optimized to effectively remove the residual lipid in the protein solution.

In order to solve the technical problems, the invention provides the following technical scheme: a method for preparing high-protein content linseed protein by aqueous medium method comprises,

adding water into a flaxseed raw material to carry out wet grinding, adjusting the pH of a product obtained after the wet grinding to 7.0-10.0, stirring and extracting, and filtering to remove residues to obtain flaxseed pulp;

centrifuging the flaxseed slurry a first time to obtain a first light phase, an intermediate phase and a first heavy phase;

adjusting the pH value of the intermediate phase to 5.5-8.5, adjusting the salt concentration to 0-0.1 mol/L, and performing second centrifugal separation at the temperature of 4-20 ℃ to obtain a second light phase and a second heavy phase;

adjusting the pH value of the second heavy phase to 7.0-7.5, and then carrying out spray drying to obtain linseed protein concentrate;

adjusting the pH value of the second light phase to 4.0-5.0, and performing centrifugal separation for the third time to obtain curd; and adjusting the pH value of the curd to 7.0-7.5, and then carrying out spray drying to obtain the linseed protein isolate.

As a preferable aspect of the present invention, wherein: the flaxseed raw material is full-fat dehulled flaxseed or cold-pressed dehulled flaxseed meal.

As a preferable aspect of the present invention, wherein: and adding water for wet pulping, wherein the water adding amount is 0.5-5 times of the weight of the raw materials.

As a preferable aspect of the present invention, wherein: the stirring extraction time is 0.5-2 h.

As a preferable aspect of the present invention, wherein: filtering to remove residues, washing the filtered residues with water, filtering again, repeating for 1-3 times, and combining the obtained filtrates to obtain the linseed pulp;

wherein the total amount of the added water is 10-30 times of the weight of the raw materials.

As a preferable aspect of the present invention, wherein: and (3) performing the first centrifugal separation, namely separating by using a three-phase disc centrifuge under 1000-5000 g.

As a preferable aspect of the present invention, wherein: and performing the second centrifugal separation, namely separating by using a two-phase disc centrifuge at 3000-12000 g.

As a preferable aspect of the present invention, wherein: and performing third centrifugal separation, namely separating by using a horizontal spiral centrifuge at 1500-3000 g.

As a preferable aspect of the present invention, wherein: and further comprising the steps of adjusting the pH value of the first light phase to 5.0-7.0, freezing, thawing, and centrifuging for the fourth time to obtain the linseed oil.

As a preferable aspect of the present invention, wherein: the freezing treatment is carried out for 5-18 h at the temperature of-30 to-5 ℃;

the unfreezing treatment is to unfreeze at the ambient temperature of 10-50 ℃;

and performing centrifugal separation for the fourth time, namely separating by using a three-phase disc centrifuge at 3000-10000 g.

The invention has the beneficial effects that: the method comprehensively utilizes the flaxseeds, is low in temperature in the whole process, is more green and safe compared with the prior art without introducing organic solvents and enzyme preparations, and realizes high-value utilization of the flaxseeds; by controlling the pH, salt concentration and temperature of the protein liquid and optimizing conditions, the residual lipid in the protein liquid can be effectively removed, so that the residual lipid can be separated under a lower centrifugal force, the protein content of the final product is more than 90%, and meanwhile, low-content protein products and linseed oil can be recovered.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, specific embodiments thereof are described in detail below with reference to examples of the specification.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.