Chinese herbal medicine feed biological additive, biological feed and preparation method
阅读说明：本技术 一种中草药饲料生物添加剂、生物饲料及制备方法 (Chinese herbal medicine feed biological additive, biological feed and preparation method ) 是由 刘华光 于 2020-05-21 设计创作，主要内容包括：本发明公开了一种中草药饲料生物添加剂、生物饲料及制备方法,所述饲料添加剂包括藤茶素及若干种中草药,中草药包含有艾叶粉,蒲公英,麻黄,黄芩,柴胡,陈皮,鱼腥草,柴胡,夏枯草,青蒿粉,雄黄粉,连翘,甘草,防风,野菊花,陈皮,白花蛇,贝母,白花蛇,牛蒡子,百部等。并配置包含有所述中草药饲料生物添加剂饲料配方。本发明通过利用植物提取技术获得的中草药成分,按配方比例添加进禽畜口粮中使用,以达到防病于治,增强畜禽免疫力的同时,代替或部分代替广谱药物(抗生素)的使用,有效解决禽畜药物残留问题。(The invention discloses a Chinese herbal medicine feed biological additive, a biological feed and a preparation method thereof, wherein the feed additive comprises ampelopsin and a plurality of Chinese herbal medicines, and the Chinese herbal medicines comprise folium artemisiae argyi powder, dandelion, ephedra, scutellaria baicalensis, radix bupleuri, pericarpium citri reticulatae, houttuynia cordata, radix bupleuri, selfheal, sweet wormwood powder, realgar powder, fructus forsythiae, liquorice, radix sileris, wild chrysanthemum flower, pericarpium citri reticulatae, long-noded pit viper, fritillaria, long-noded pit viper, burdock, radix stemonae and the. And preparing a feed formula containing the Chinese herbal medicine feed biological additive. The invention uses the Chinese herbal medicine components obtained by the plant extraction technology, and is added into the livestock food according to the formula proportion for use, so as to achieve the purposes of preventing and treating diseases, enhancing the immunity of livestock and poultry, and simultaneously replace or partially replace the use of broad-spectrum medicines (antibiotics), thereby effectively solving the problem of drug residue of livestock and poultry.)
1. A Chinese herbal medicine feed biological additive is characterized in that: comprises ampelopsin and a plurality of Chinese herbal medicines.
2. The Chinese herbal medicine feed biological additive according to claim 1, which is characterized in that: the Chinese herbal medicine comprises one or more of folium Artemisiae Argyi powder, herba Taraxaci, herba Ephedrae, Scutellariae radix, bupleuri radix, pericarpium Citri Tangerinae, herba Houttuyniae, bupleuri radix, Prunellae Spica, herba Artemisiae Annuae powder, Realgar powder, fructus forsythiae, Glycyrrhrizae radix, radix Saposhnikoviae, flos Chrysanthemi Indici, pericarpium Citri Tangerinae, Agkistrodon, Bulbus Fritillariae Cirrhosae, fructus Arctii, and radix Stemonae.
3. The Chinese herbal medicine feed biological additive according to claim 2, which is characterized by being prepared from the following components in parts by weight:
4. the Chinese herbal medicine feed biological additive according to claim 3, which is characterized by being prepared from the following components in parts by weight: 50 parts of ampelopsin, 20 parts of folium artemisiae argyi powder, 18 parts of dandelion, 12 parts of ephedra, 15 parts of scutellaria baicalensis, 12 parts of radix bupleuri, 8 parts of pericarpium citri reticulatae, 15 parts of houttuynia cordata, 12 parts of radix bupleuri, 12 parts of selfheal, 15 parts of sweet wormwood powder, 8 parts of realgar powder, 16 parts of fructus forsythiae, 10 parts of liquorice, 12 parts of radix sileris, 12 parts of wild chrysanthemum flower, 13 parts of long-noded pit viper, 12 parts of fritillaria, 10 parts of burdock and 12 parts of radix stemonae.
5. A biological feed comprising the compound of claim 1, characterized by comprising: chinese herbal medicine feed biological additive, pennisetum hydridum, fermented feed, corn flour, wheat bran, bean flour, shells, fishbone powder, epimedium, malt flour, pine needle powder and garlic residue.
6. The Chinese herbal medicine feed biological additive feed as claimed in claim 5, which is characterized in that: the Chinese herbal medicine feed biological additive accounts for the following feed weight ratio ranges: 0.5-1.2 kg: 1000 kg.
7. A method of preparing a Chinese herbal feed additive for poultry feed according to claim 3, characterized in that: the method comprises the following steps:
step S1: grinding the raw materials into powder according to a ratio;
step S2: mixing the powder of S1 with purified water at a ratio of 1:20, adding into a medical container, boiling with strong fire, decocting with slow fire to obtain brown liquid, cooling, and placing in a refrigerator.
Step S3: diluting the brown liquid substance in the S2 with purified water at a ratio of 1:5, and filtering to obtain aqueous solution;
step S4: cooling the water aqua in the S3, bagging the water aqua in a refrigerator for later use, and directly drenching the chicks;
step S5: drying the brown liquid in the S2 at high temperature to obtain powder, and packaging in a dry place for adding into biological feed.
8. The method of claim 7, wherein: in step S2, the temperature of the strong fire is 120-150 ℃.
9. The method of claim 7, wherein: in step S5, the brown liquid is dried at a high temperature of 180-200 ℃.
Technical Field
The invention belongs to the technical field of Chinese herbal medicine biological feed additives and biological feeds, and particularly relates to a Chinese herbal medicine feed biological additive for poultry feed, a biological feed and a preparation method
Background
In 1950, the Food and Drug Administration (FDA) of the United states approved for the first time to add broad-spectrum antibacterial drugs (antibiotics) into the feed, which can promote the growth of livestock and poultry, and later, the world countries have been subjected to feeding tests of adding broad-spectrum antibacterial drugs (antibiotics) and used for production.
Tetracycline antibiotics (TCs) are most widely applied in a plurality of broad-spectrum antibiotic medicines, and comprise tetracycline, terramycin, aureomycin and doxycycline, and are used as animal health antibiotics, have good bactericidal action at higher concentration, are favored by farmers, and are widely applied to treatment of edible animal diseases and medicament additives. The variety of the added broad-spectrum antibacterial drugs (antibiotics) which are allowed to be used as feed additives in China has 3 types and 30 types, strict regulations are made on the minimum dosage, the maximum dosage and the drug-withdrawal period of applicable animals, and consumers do not need to worry about the broad-spectrum antibacterial drug components in a reasonable range from chicken and eggs in general.
However, in many chicken farms and individuals, because of more chicken diseases, in order to not affect economic benefits, a large dose of broad-spectrum antibacterial drugs are often used for a long time, livestock and poultry are often injected or fed with antibiotic drugs before long-distance transportation to reduce losses caused by fatalities in transportation, the broad-spectrum antibacterial drugs are used for a long time exceeding standard, environment deterioration is directly caused, meanwhile, the health of people is seriously affected, for example, tetracycline has great influence on the growth of bones and teeth of newborns, and can be transited to infants through mothers, and long-time use of tetracycline can cause the reduction of normal flora of animal organisms such as livestock and poultry, cause vitamin deficiency, cause fungal reproduction, or cause double infection, and the data shows according to medical observation that: antibiotic drugs can also cause skeletal deformity, causing liver and kidney damage, allergic reactions, allergies, drug resistance, and the like. Therefore, the problem of drug residues of livestock and poultry has become a social topic of food safety.
Developing high-quality, safe and high-efficiency feed products. Popularizing safe and environment-friendly feed products. The nutrition balance technology and the novel feed additive product are comprehensively applied, the technology of the formula of the low-nitrogen, low-phosphorus and low-trace element discharge feed is researched and developed, the environment-friendly feed product is popularized, and the discharge reduction of culture pollutants is promoted. The novel safe feed additive products such as microbial agents, plant extracts and the like are comprehensively utilized, and the use of medicinal feed additives such as poultry and livestock antibiotics and the like is reduced. Therefore, the research and development of the Chinese herbal medicine feed additive which can replace broad-spectrum antibiotic medicines, has no medicine residue, no drug resistance, no side effect and growth promoting effect is concerned by national stress and the feed industry.
The invention provides a low-toxicity, green and pollution-free Chinese herbal medicine feed biological additive, a biological feed and a preparation method thereof, aiming at solving various damages to the growth of poultry caused by adding various broad-spectrum antibacterial drugs (antibiotics) into a feed additive.
Disclosure of Invention
In order to achieve the purpose, the invention provides a Chinese herbal medicine feed biological additive which is used for a livestock feed additive and comprises ampelopsin (commonly known as dihydromyricetin) and a plurality of Chinese herbal medicines, and the additive is low in toxicity, green and pollution-free and does not contain any antibiotic.
The invention also provides a formula containing the Chinese herbal medicine feed biological additive and a biological feed and a preparation method thereof, the Chinese herbal medicine feed biological additive is added into the biological feed according to the formula proportion, so that the purposes of preventing and treating diseases and enhancing the immunity of livestock and poultry are achieved, and meanwhile, the use of broad-spectrum medicines (antibiotics) is replaced or partially replaced, and the problem of drug residue of the livestock and poultry is effectively solved.
Preferably, in the Chinese herbal medicine feed biological additive, the Chinese herbal medicine comprises one or more of folium artemisiae argyi powder, dandelion, ephedra herb, scutellaria baicalensis, radix bupleuri, pericarpium citri reticulatae, houttuynia cordata, radix bupleuri, selfheal, sweet wormwood powder, realgar powder, fructus forsythiae, liquorice, radix sileris, wild chrysanthemum, pericarpium citri reticulatae, long-noded pit viper, fritillaria, long-noded pit viper, burdock and radix stemonae.
Preferably, in the Chinese herbal medicine feed biological additive, the weight parts of the components are as follows:
preferably, the Chinese herbal medicine feed biological additive is prepared from the following components in parts by weight: 50 parts of ampelopsin, 20 parts of folium artemisiae argyi powder, 18 parts of dandelion, 12 parts of ephedra, 15 parts of scutellaria baicalensis, 12 parts of radix bupleuri, 8 parts of pericarpium citri reticulatae, 15 parts of houttuynia cordata, 12 parts of radix bupleuri, 12 parts of selfheal, 15 parts of sweet wormwood powder, 8 parts of realgar powder, 16 parts of fructus forsythiae, 10 parts of liquorice, 12 parts of radix sileris, 12 parts of wild chrysanthemum flower, 13 parts of long-noded pit viper, 12 parts of fritillaria, 10 parts of burdock and 12 parts of radix stemonae.
Preferably, said biological feed comprises: chinese herbal medicine feed biological additive, pennisetum sinese roxb, fermented feed, corn flour, wheat bran, bean flour, shell, fishbone powder, epimedium herb, malt flour, pine needle powder, garlic residue and the like. The fermented feed is Taiwan sweet grassiness green fermented feed.
Preferably, the weight ratio of the non-antibiotic agent to the feed additive is: 0.5-1.2 kg: 1000 kg.
The feed additive accounts for the following feed in percentage by weight:
the invention also provides a method for preparing the ampelopsis grossedentata antimicrobial-free agent, which comprises the following steps:
step S1: grinding the raw materials into powder according to a ratio;
step S2: mixing the powder of S1 with purified water at a ratio of 1:20, adding into a medical container, boiling with strong fire, decocting with slow fire to obtain brown liquid, cooling, and placing in a refrigerator.
Step S3: diluting the brown liquid substance in the S2 with purified water at a ratio of 1:5, and filtering to obtain aqueous solution;
step S4: cooling the water aqua in the S3, bagging the water aqua in a refrigerator for later use, and directly drenching the chicks;
step S5: drying the brown liquid in the S2 at high temperature to obtain powder, and packaging in a drying place for adding biological feed;
in the step S2, the temperature of the strong fire is 120-150 ℃. In step S5, the brown liquid is dried at a high temperature of 180-200 ℃.
The invention has the beneficial effects that: the invention provides a low-toxicity, green and pollution-free Chinese herbal medicine feed biological additive, biological feed and a preparation method thereof.
Detailed Description
The invention discloses a low-toxicity, green and pollution-free Chinese herbal medicine feed biological additive, which is used in livestock feed additives and comprises ampelopsin (commonly known as dihydromyricetin) and a plurality of Chinese herbal medicines. Wherein the Chinese herbal medicine comprises one or more of folium Artemisiae Argyi powder, herba Taraxaci, herba Ephedrae, Scutellariae radix, bupleuri radix, pericarpium Citri Tangerinae, herba Houttuyniae, bupleuri radix, Prunellae Spica, herba Artemisiae Annuae powder, Realgar powder, fructus forsythiae, Glycyrrhrizae radix, radix Saposhnikoviae, flos Chrysanthemi Indici, pericarpium Citri Tangerinae, Agkistrodon, Bulbus Fritillariae Cirrhosae, Agkistrodon, fructus Arctii, and radix Stemonae.
The Chinese herbal medicine feed biological additive comprises the following components in parts by weight:
in a preferred embodiment of the formula of the antimicrobial-free agent, the antimicrobial agent comprises 50 parts of ampelopsin, 20 parts of folium artemisiae argyi powder, 18 parts of dandelion, 12 parts of ephedra, 15 parts of scutellaria baicalensis, 12 parts of radix bupleuri, 8 parts of pericarpium citri reticulatae, 15 parts of fishy grass, 12 parts of radix bupleuri, 12 parts of selfheal, 15 parts of sweet wormwood powder, 8 parts of realgar powder, 16 parts of fructus forsythiae, 10 parts of liquorice, 12 parts of radix sileris, 12 parts of wild chrysanthemum flower, 13 parts of long-noded pit viper, 12 parts of fritillaria, 10 parts of burdock and 12 parts of radix stemonae.
First, experiment 1: a comparative experiment based on the formula effect of Chinese herbal medicine feed additive.
35 parts of ampelopsin, 15 parts of folium artemisiae argyi powder, 20 parts of houttuynia cordata, 15 parts of radix bupleuri, 12 parts of selfheal, 15 parts of sweet wormwood powder, 12 parts of realgar powder, 15 parts of fructus forsythiae, 12 parts of liquorice, 10 parts of divaricate saposhnikovia root, 15 parts of wild chrysanthemum, 15 parts of dried orange peel, 12 parts of long-noded pit viper and 15 parts of ginseng rootlets. The 14 Chinese herbal medicines are mixed and ground into powder to prepare the medicament according to the following flow.
1) Grinding the 14 medicines into powder, putting into an iron pan, boiling with strong fire, decocting with slow fire, decocting with standard 1000g sea bowl 5 bowls of water to 1 bowl with orange brown color, cooling, bagging, and storing in refrigerator for use.
Water aqua: 10 days for 1-30 days; the age of the chickens is 5 days per dose in 31-60 days;
powder preparation: mixing 61-120 days (including more than 120 days) of powdered chicken with the feed according to the proportion of 1000 kg: 0.5 kg.
2) The age of the chickens is 1-10 days. The medicine is manually infused for 10 days continuously, 3-5 drops are injected into each chicken, the medicine is proportionally mixed into drinking water of the livestock from the 11 th to 30 th days, and the drinking water is added for a plurality of times to ensure that the drinking water is finished in the same day.
3) The age of the chicken is 31-60 days. The drinking water is continuously fed with Chinese medicinal materials for several times until the feather wing of chicken grows.
The self-made green fermented feed formula comprises the following components:
4) the chicken ages of 61-120 days. The drinking water source does not need to add medicament, and the medicament powder is directly mixed into the feed for direct feeding.
11/2018 month 03 to 11/2018 month 7. Randomly selecting 2000 Qingyuan partridge chickens with the age of 1 week, dividing the Qingyuan partridge chickens into 1000 control groups and 1000 test groups, and respectively selecting chickens with the age of 1-45 days (young chickens) and chickens with the age of 46-90 days (young chickens); the chickens aged 91-135 days (finished chickens) are continuously intervened for 3 times, and each intervention is an experiment in a 45-day period. The experimental group is cultured by adopting a Chinese herbal medicine feed additive technology, and the control group is cultured by adopting a broad-spectrum antibacterial medicine technology.
The company research and development team li over supervise brings scientific research personnel Liuhuaguan, Lifeng and Wuqiao to take full charge of detection work with 12 students of Guangdong department trade vocational college, under the same feeding and management conditions, national basic prevention vaccines and nutritional medicines at each age stage are unchanged, 45 days are a stage, 2 random samples are taken from an experimental group and a control group at each stage for dissection, according to GB/T20764-2006, an HPLC detection method is adopted, the detection work is finished in provincial animal laboratories of the white cloud region stone well school zone of the Guangdong department trade vocational college, and the residual degrees of oxytetracycline, tetracycline, aureomycin and doxycycline are respectively analyzed.
1. Preparation of the experiment
1.1 instrumentation
A high-efficiency liquid-phase chromatographic band ultraviolet detector (model LC 2010); a chromatography column (CAPCELLPAKC18MG iii); a nitrogen blower (103199); a vortex oscillator (KS 501); a high speed refrigerated centrifuge (CR 21G); analytical electronic balance (model FA 2004N); a mashing machine (model JJ-2B); a solid phase extraction device; Milli-Q type ultrapure water meter; a 50mL liquid reservoir; a 10mL graduated sample tube; a vacuum pump; a 100mL flat bottom flask; and a pH meter.
1.2 materials and reagents
Methanol; acetonitrile; ethyl acetate; disodium hydrogen phosphate; citric acid (C)6H8O7·H2O); disodium ethylene diamine tetraacetic acid (Na)2EDTA·2H2O); oxalic acid; a disodium hydrogen phosphate solution; a citric acid solution; mclvaine buffer solution; na (Na)2EDTA-Mclvaine buffer solution; methanol + water (1+ 19); mobile phase: acetonitrile + methanol +0.01mol/L oxalic acid solution (2+1+ 7).
Oxytetracycline, tetracycline, chlortetracycline, doxycycline standard substances: purity is more than or equal to 95%, and standard stock solution: the standard stock solutions were prepared separately in 0.1mg/mL methanol. The stock solution was stored at-18 ℃.
Mixing standard working solutions of oxytetracycline, tetracycline, chlortetracycline and doxycycline: according to the requirements, the terramycin, tetracycline, aureomycin and doxycycline are diluted into mixed standard working solutions with different concentrations of 5ng/mL, 10ng/mL, 50ng/mL, 100ng/mL and 200ng/mL by using a mobile phase
And respectively feeding the mixed standard working solution, drawing a standard working curve by taking the concentration as a horizontal coordinate and the peak area as a vertical coordinate, and quantifying the sample by using the standard working curve, wherein the response values of the oxytetracycline, the tetracycline, the aureomycin and the doxycycline in the sample solution are all in the linear range measured by an instrument. The reference retention times of oxytetracycline, tetracycline, chlortetracycline, doxycycline under the chromatographic conditions described above are shown in the table.
Reference retention time of oxytetracycline, tetracycline, chlortetracycline and doxycycline
Name of drug
Retention time/min
Oxytetracycline
4.87
Tetracycline derivatives
5.52
Aureomycin
1.22
Doxycycline
15.25
Oasisch solid phase extraction column or equivalent: 500mg, 3 mL. The cartridge was kept wet by pretreatment with 5mL of methanol and 10mL of water, respectively, prior to use.
Cation exchange column: carboxylic acid type, 500mg, 3 mL. The cartridge was kept wet by pre-treatment with 5mL ethyl acetate before use.
2. Test procedure and results
2.1 preparation of Standard solution
Precisely weighing 4 parts of four tetracycline antibiotics reference substances, wherein each part is about 0.01g, preparing into a 10mL measuring flask, adding hydrochloric acid to dissolve and dilute to scale, shaking up to obtain standard stock solution, and freezing for storage.
2.2 conditions of high Performance liquid chromatography and Mass Spectrometry
Chromatographic separation conditions:
1) a chromatographic column: mightsil RP-18GP, 3um, 150mm × 4.6mm or equivalent;
2) mobile phase: acetonitrile + methanol +0.01mol/L oxalic acid solution (2+1+ 7);
3) flow rate: 0.2 mL/min;
4) column temperature: 20 ℃;
5) detection wavelength: 350 nm;
6) sample introduction amount: 20 uL.
7) Gradient elution procedure: keeping 10% of B for 0.0-1.0 min, keeping for 1.0-4.0 min, linearly increasing 10% of B to 75%, decreasing to 10% of B for 4.1min, and keeping for 8.0 min;
mass spectrum conditions:
ionization mode: ESI +;
capillary voltage: 4000 v;
the detection mode is as follows: multiple Reaction Monitoring (MRM);
atomization temperature: 200 ℃;
temperature source: 80 ℃;
electrospray pressure: at a pressure of 30psi, and,
flow rate of drying gas: 9L/min of the water-soluble organic solvent,
multiplication voltage: 300EMV (+).
Phosphate solution (pH 8.0)
2.3 sample extraction
Taking 10g of each sample, mashing by a tissue mashing machine (less than 5mm), fully stirring, uniformly mixing, respectively filling into a clean container, and sealing to obtain a sample. Cryopreservation of 2H at-20 ℃.6 g. + -. 0.01g of the sample is weighed into a 50mL stoppered centrifuge tube and 25mL of a buffer solution of 5% perchloric acid is added. Homogenizing for 1min, and performing ultrasonic treatment in ice water bath for 40 min. Centrifuging the extractive solution at 8000r/min for 10min, and pouring the supernatant into another centrifuge tube. The residue was extracted once more with 20mL of a buffer solution, and the supernatants were combined.
2.4 purification
Adding the extracted supernatant into an Oasis HLB solid phase extraction column, allowing the supernatant to pass through the solid phase extraction column at a flow rate of 3mL/min, washing the column with 5mL of methanol + water (1+19) after the supernatant completely flows out, and vacuum-drying under a negative pressure of 65kPa for 3 min. Eluting with acetonitrile, methanol and 0.01mol/L oxalic acid solution (2+1+7), and collecting the eluent in a 5mL sample tube. The volume of the solution was reduced to approximately 1mL (temperature below 30 ℃ C.) by blowing nitrogen gas, and the volume was reduced to 4 mL. Filtering with 13mm, 0.22 μm PTFE needle filter, and measuring with liquid chromatography-ultraviolet detector.
2.5 assay data analysis
Respectively weighing 1 part of each of experimental chicken samples (chicken, chicken liver, chicken kidney and chicken spleen) and control chicken samples (chicken, chicken liver, chicken kidney and chicken spleen) for 1-45 days, 46-90 days and 91-135 days, wherein each part is 10g, and 24 parts of the samples are used for determining residues of the four antibiotics in the chicken and carrying out qualitative and quantitative analysis. The experiment mainly aims at the determination of the residue of the oxytetracycline and the tetracycline in chicken. The highest allowable residual quantity in the tissues of muscles, livers, spleens and kidneys specified by the European Community is 0.5 mg/kg.
TABLE I, content detection of oxytetracycline and tetracycline for 1-45 daysmg/kg
Second, the content of oxytetracycline and tetracycline is detected for 46 to 90 daysmg/kg
Detection of oxytetracycline and tetracycline content in days 91-135mg/kg
And (3) respectively analyzing the residual degrees of the oxytetracycline and the tetracycline in 24 samples of the chicken, the chicken liver, the chicken kidney and the chicken spleen in the 3 stages by adopting an HPLC (high performance liquid chromatography) detection method. The experimental group is cultured by adopting a Chinese herbal medicine feed additive technology, and the control group is cultured by adopting a broad-spectrum antibacterial medicine technology.
3. Analysis of experiments
3.1 survival rate analysis:
the experimental group adopts the Chinese herbal medicine feed additive technology to breed 113 deaths, the survival rate is 88.7%, the broad-spectrum antibacterial technology is adopted to breed 84 deaths, the survival rate is 91.6%, the difference between the two is 2.9%, the difference is very obvious, and the reason why farmers favor the broad-spectrum antibacterial technology for breeding is fully demonstrated.
The survival rate of the cultivation by the Chinese herbal medicine feed additive technology is 88.7 percent, which also indicates that the cultivation by the Chinese herbal medicine feed additive technology is effective, and the formula effect can be improved in a space if the formula effect is corrected in the future.
3.2 antibiotic residue analysis:
by adopting a Chinese herbal medicine feed additive culture technology, the highest oxytetracycline residue is 1.19mg/kg, and the lowest oxytetracycline residue is 0.58 mg/kg; the highest residual amount of tetracycline is 1.74mg/kg, and the lowest residual amount of tetracycline is 0.83 mg/kg;
the method adopts broad-spectrum antibacterial technology for cultivation, and the highest oxytetracycline residue is 6.32mg/kg, and the lowest oxytetracycline residue is 1.74 mg/kg;
the maximum tetracycline residue is 4.32mg/kg, and the minimum tetracycline residue is 2.23 mg/kg;
obviously, the terramycin residue in the Chinese herbal medicine feed additive culture technology is 5.13mg/kg lower than that in the broad-spectrum antibacterial medicine technology; the residual tetracycline is lower than 3.49mg/kg of the wide-spectrum antibacterial drug technology;
the problem of residue can be greatly reduced by adopting Chinese herbal medicine health care medicines to replace antibiotics.
3.3 cost of medication analysis
Within 1-45 days, the chicken can be used for preventing newcastle disease, infectious bronchitis, bursal disease, intestinal tract disease and respiratory disease.
The cost of the Chinese patent medicine used by the test group in the 45-day-old stage is 1297 yuan.
The cost of using broad-spectrum antibacterial drugs in the 45-day-old stage of the control group is 1846 yuan.
Therefore, compared with a control group, the cost of the preventive medicine is reduced by 549 yuan, and the cost is reduced by 29.73%.
The above experiments show that it is feasible to replace broad-spectrum antibacterial drugs (antibiotics) with Chinese herbal medicine biological health care agents in large-scale chicken raising production.
Firstly, the problem of drug residue in the chicken is solved, and the food safety is kept in the production link.
Secondly, 2 reduced experimental targets are realized, namely, the medication cost is reduced and the residual rate is reduced.
Thirdly, the survival rate of the Chinese herbal medicine feed additive is lower than 90%, the wide popularization is far from enough, and further experiments and formula correction are needed.
The present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents and are included in the scope of the present invention.
Second, experiment 2: a Chinese herbal medicine feed additive for substituting broad-spectrum antibacterial drug formula correction test is provided.
The modified formula comprises the following components: 50 parts of ampelopsin, 15 parts of dandelion, 15 parts of houttuynia cordata, 6 parts of radix bupleuri, 6 parts of selfheal, 15 parts of sweet wormwood powder, 15 parts of cortex mori, 12 parts of realgar powder, 20 parts of fructus forsythiae, 12 parts of almond, 10 parts of divaricate saposhnikovia root, 30 parts of wild chrysanthemum flower, 15 parts of dried orange peel, 6 parts of long-noded pit viper and 6 parts of ginseng rootlets. The 14 Chinese herbal medicines are mixed and ground into powder to prepare the medicament according to the following flow. The 14 medicines are 1 prescription, and are mixed and ground into powder to prepare the powder preparation according to the method of the first experiment. A recipe correction test was performed.
1. Materials and methods
1.1 test site and team
Guangdong phoenix four meeting river valley town farm (Guangdong academy of sciences research and development 5 people)
1.2 test grouping
400 feathers of chicks (black-bone chickens) which are out of the shell for 3 weeks are randomly divided into a test group and a control group, and each group has 200 feathers to carry out a brooding test. 0.5kg of Chinese herbal medicine feed additive is added into 1000kg of basic grains of a test group, and the basic grains are continuously administrated for 5 days, and then administrated for 1 time every 3 days, and 4 times in 1 month. The control group was injected or added with a broad spectrum drug as usual.
1.3 Experimental feeds
Self-made green fermented feed
1.4 test time
Between 08 and 07 days 2018 and 09 and 23 days 2018
1.5 Breeding management
In the test period, the basic ration of 2 groups and the feeding environment conditions and management are the same, and the group is fed by the same feeder and is free to drink water. Basic daily ration nutrient components: 21% of crude protein in brooding period, 0.48% of methionine, 1.20% of lysine, 0.75% of total phosphorus, 1.05% of calcium and 2.00% of crude fiber; the immunization program is carried out according to the immunization program recommended by the handbook of raising and managing commercial laying hens.
2. Results of the experiment
The test group had 26 cases with 13% morbidity, 19 deaths and 9.5% mortality. The protection rate is 90.5%. The control group had 17 cases with 8.5% morbidity, 14 deaths, and 5.5% mortality. The protection rate is 94.5%. 0.5kg of Chinese herbal medicine feed additive is added into 1000kg of basic grains of the test group, and the basic grains are continuously administrated for 5 days, and then administrated for 1 time every 3 days, and 4 times in 1 month.
The control group was injected or added with conventional broad spectrum drug 4 times.
Results of the experiment
Group of
Number of breeding animals
Weight gain
Number of onset of disease
Number of deaths
Survival rate
Experimental group
200
184.5g±6.5A
26
19
90.50
Control group
200
169.3g±8.3B
17
14
94.50
Total of
400
--
43
33
91.75
The same column is marked with the same letter to indicate that the difference is not significant (P is more than 0.05); the difference of different small-case letters is obvious (P < 0.05), and the difference of different capital letters is obvious (P < 0.01)
According to the data obtained by investigation, through the biological statistical analysis, as can be seen from table 1, the weight gain of each feather of the test group and the control group is very obvious in difference (less than 0.01) in the test period; the difference in the number of deaths was very significant (< 0.01), 19 feathers died in the test group with a mortality rate of 9.5% (19/200), 14 feathers died in the control group with a mortality rate of 5.5% (14/200), and the mortality rate of the test group was 3% different from that of the control group and was 1.8% higher than that of 88.7% before correction.
3. Analysis of experiments
1) The experimental results show that 0.5kg of Chinese herbal medicine feed additive is added into 1000kg of basic grains in the experimental group, the basic grains are continuously administrated for 3 days, then the basic grains are administrated for 1 time every 5 days, the total time is 4 times in 1 month, and the weight gain of each feather of the experimental group and the control group is very obvious (less than 0.01); the difference of death numbers is extremely obvious (less than 0.01), the activity rate of the tested composition reaches 90.5 percent, is improved by 1.8 percent compared with 88.7 percent before correction, and the prescription effect after correction is more obvious.
2) The vine tea extract component is increased from 35g to 50g, which shows that the vine tea extract has obvious effect and has the functions of antibiosis, antivirus, anti-inflammation, immunity regulation and the like; fructus forsythiae has effects in clearing away heat and toxic materials, relieving exterior syndrome, and also has antibacterial, antiviral, antiinflammatory, immunity regulating, analgesic, and antipyretic effects; the coptis chinensis dispels wind, relieves exterior syndrome, reduces swelling, eliminates stagnation, tonifies qi and tranquilizes the mind, relieves cough and asthma, and contains various bioactive substances; the ginseng rootlets can greatly tonify primordial qi, recover pulse, relieve depletion, tonify spleen and lung, promote salivation, calm mind and improve the immunity of the organism; the composition is used as a ministerial drug. The main common effects are to kill or inhibit pathogenic microorganisms and promote the health of organisms.
3) The corrected Chinese herbal medicine preparation has the characteristics of no drug resistance, no residue, no side effect and the like, can enhance the immunity of an organism, promote the development of immune organs, regulate the nonspecific immune function of the organism, and has the antibacterial and antiviral effects, so the mechanism of the function is further researched, and the ecological non-resistant cultivation has important research value.
Third, experiment 3: an optimization test of a formula of a Chinese herbal medicine feed additive for replacing a broad-spectrum antibacterial agent.
The final formulation ingredients were as follows: 50 parts of ampelopsin, 20 parts of folium artemisiae argyi powder, 18 parts of dandelion, 12 parts of ephedra, 15 parts of scutellaria baicalensis, 12 parts of radix bupleuri, 8 parts of pericarpium citri reticulatae, 15 parts of houttuynia cordata, 12 parts of radix bupleuri, 12 parts of selfheal, 15 parts of sweet wormwood powder, 8 parts of realgar powder, 16 parts of fructus forsythiae, 10 parts of liquorice, 12 parts of radix sileris, 12 parts of wild chrysanthemum flower, 13 parts of long-noded pit viper, 12 parts of fritillaria, 10 parts of burdock and 12 parts of radix stemonae.
1. Test site and team
The Jiulong gang tea cultivation base of Guangdong Fenghuang ecological agriculture Limited company is completed by 5 people in the research and development team of Guangdong Kongmao professional college.
2. Test time
The experimental period is three months (90 days), and 3 days from 03 month and 18 days in 2019 to 6 months and 18 days in 2019
Self-made green fermented feed
4. Test grouping
3.5 ten thousand laying hens in a nine-dragon-hillock ampelopsis tea cultivation base are kept in 5 henhouses, each house is divided into 5 groups according to natural henhouses, one of the groups is a comparison group, and a broad-spectrum drug antibiotic additive is used; four test groups, each group was equipped with 2 trainees for tracking and data acquisition, and added to the feed according to the Chinese herbal medicine biological additives of 0.5%, 0.6%, 0.7% and 0.8%, and the data comparison was as follows:
5. test results
The test result shows that the difference between the laying rate of the test group using the herbal medicine biological additive and the laying rate of the comparison group is not obvious, the average laying rate of the test group is 87.15 percent, the average laying rate of the comparison group is 87.05 percent, the percentage of the test group is 0.1 percent higher than that of the comparison group, the statistical test P is more than 0.05, and the difference is not obvious; however, the number of the chickens died of the disease and the disease death rate of the test group are lower than those of the control group, particularly the test three groups are most obvious, 61 chickens died of the test group have the disease death rate of 0.087 percent, 61 chickens in the control group have the disease death rate of 0.087 percent, and the Chinese herbal medicine biological additive is basically equivalent to the broad-spectrum medicine.
6. Analysis of experiments
The conclusion of the test is as follows: the laying rate of the control group and the test group is not greatly different by using the Chinese herbal medicine biological additive as the laying hen feed additive; the number of the dead chickens in the test group and the fatality rate of the chickens in the test group are leveled up for the first time, and particularly the test three groups have better performance. Therefore, the Chinese herbal medicine biological additive used as the feed additive has the functions of enhancing the immunity and disease resistance of the chickens.
The Chinese herbal medicine biological additive has the characteristics of no drug resistance, no residue, no side effect and the like, can enhance the immunity of an organism, promote the development of immune organs, regulate the nonspecific immunity function of the organism, and has antibacterial and antiviral effects, so that the mechanism of the action is further researched, the Chinese herbal medicine biological additive has important practical significance for establishing a novel sustainable development mode of safety, environmental protection and health, and is widely popularized.