阅读说明：本技术 白屈菜红碱在制备抑制白色念珠菌和金黄色葡萄球菌双物种生物被膜药物中的应用 (Application of chelerythrine in preparation of medicines for inhibiting biological envelopes of candida albicans and staphylococcus aureus ) 是由 钱卫东 张家宁 王文静 刘淼 孙照欢 杨敏 付玉婷 李鑫辰 于 2019-12-13 设计创作，主要内容包括：本发明公开了白屈菜红碱在制备抑制白色念珠菌和金黄色葡萄球菌双物种生物被膜药物中的应用,本发明采用白色念珠菌与金黄色葡萄球菌双物种生物被膜体外模型研究白屈菜红碱对双物种生物被膜的体外干预作用；通过结晶紫法观察发现白屈菜红碱能有效干预白色念珠菌和金黄色葡萄球菌生物被膜的形成,通过场发射扫描电子显微镜观察发现白屈菜红碱对生物被膜内的白色念珠菌与金黄色葡萄球菌有显著的抑制与清除作用。因此,白屈菜红碱对于抑制白色念珠菌和金黄色葡萄球菌双物种生物被膜有确切的功效,能应用于制备抑制或清除白色念珠菌和金黄色葡萄球菌双物种生物被膜的药物,具有有效意义。(The invention discloses an application of chelerythrine in preparation of a medicament for inhibiting dual-species biofilms of candida albicans and staphylococcus aureus, and the invention adopts a dual-species biofilm in-vitro model of the candida albicans and the staphylococcus aureus to research the in-vitro intervention effect of the chelerythrine on the dual-species biofilms; the chelerythrine can effectively intervene in the formation of candida albicans and staphylococcus aureus biofilms through the observation of a crystal violet method, and the chelerythrine has obvious inhibiting and removing effects on the candida albicans and staphylococcus aureus in the biofilms through the observation of a field emission scanning electron microscope. Therefore, the chelerythrine has definite efficacy on inhibiting the biofilm of the candida albicans and the staphylococcus aureus, can be applied to preparing medicines for inhibiting or eliminating the biofilm of the candida albicans and the staphylococcus aureus, and has effective significance.)

1. Use of chelerythrine for inhibiting the growth of biofilm of Candida albicans and Staphylococcus aureus is provided.

2. The use of chelerythrine according to claim 1, wherein said candida albicans is a human source candida albicans; staphylococcus aureus is a human Staphylococcus aureus.

3. The use of chelerythrine according to claim 1, wherein said minimum concentration for inhibiting the formation of a biofilm selected from the group consisting of Candida albicans and Staphylococcus aureus is determined by crystal violet staining.

4. The use of chelerythrine according to claim 1, wherein said chelerythrine exhibits a minimum inhibitory concentration against a Candida albicans biofilm and a Staphylococcus aureus biofilm of 4 μ g/mL and 3 μ g/mL, respectively; the minimum inhibitory concentration on the biofilm of two species of candida albicans and staphylococcus aureus is 8 mug/mL.

5. The use of chelerythrine according to claim 1, wherein said chelerythrine is effective in inhibiting the growth of a biofilm selected from the group consisting of candida albicans and staphylococcus aureus, and in inhibiting the growth of a biofilm selected from the group consisting of candida albicans and staphylococcus aureus.

6. Application of chelerythrine in preparation of medicines for inhibiting and killing Candida albicans and Staphylococcus aureus double species biofilm is provided.

7. The use of chelerythrine according to claim 6, for the preparation of a medicament for inhibiting and killing two species of biofilms, Candida albicans and Staphylococcus aureus, wherein said Candida albicans is a human source Candida albicans; staphylococcus aureus is a human Staphylococcus aureus.

8. A double species biofilm drug composition for killing Candida albicans and Staphylococcus aureus is characterized by comprising chelerythrine with effective dose for preventing or treating and pharmaceutically acceptable auxiliary materials or carriers.

9. The dual species biofilm killing pharmaceutical composition of candida albicans and staphylococcus aureus as claimed in claim 8, wherein said dual species biofilm killing comprises interfering with the formation of dual species biofilm of candida albicans and staphylococcus aureus or removing mature dual species biofilm of candida albicans and staphylococcus aureus.

Technical Field

The invention belongs to the technical field of medicine and food safety, and relates to application of chelerythrine in preparation of a medicine for inhibiting double species biofilms of candida albicans and staphylococcus aureus.

Background

Candida albicans (also called Candida albicans) is a common flora in the oral cavity and is also an important conditionally pathogenic fungus in the oral cavity. Under certain conditions, various oral mucosa diseases can be caused. It is one of the most common pathogenic bacteria clinically causing deep fungal infection. Staphylococcus aureus (Staphylococcus aureus) can infect humans and various animals, causing local infection, abscess, cellulitis, and severe septicemia and even death.

With the progress of medical technology in recent years, the probability of medical implants such as catheters, artificial valves, joint replacements and the like for clinical treatment is increased, pathogenic bacteria are adhered to the surface of the medical implants to form biofilms, and the incidence of infection related to the biofilms is increased. The formation of the biofilm enables the bacteria in the biofilm to generate extremely strong drug resistance and the capability of resisting the action of an organism immune system, the drug resistance is greatly enhanced, and the treatment difficulty is increased. Candida albicans, staphylococcus aureus, are common isolates that cause infection of the associated biofilm, and sometimes as a mixed infection. At present, although some antibacterial drugs such as ciprofloxacin and azithromycin can interfere the formation of biofilms of bacteria and fungi to a certain extent, the antibacterial drugs have a single action mode and cause the drug resistance of the bacteria and the fungi after long-term use. Research shows that various traditional Chinese medicines have obvious antibacterial effect and can inhibit the formation of bacterial and fungal biofilms, and the advantage of the traditional Chinese medicines in coping with drug-resistant bacteria is disclosed. Meanwhile, the Chinese herbal medicine has the advantages and characteristics of small toxic and side effects, wide medicine source and low price, has unique advantages in the aspect of enhancing the drug resistance of bacteria, and becomes a new source for people to search for high-efficiency and low-toxicity inhibitors.

Chelidonium majus has the effects of relieving cough, promoting urination, removing toxic substances, treating dysentery, jaundice and other diseases, and chelerythrine is the main effective antibacterial component of Chelidonium majus and has antibacterial and antifungal effects. The method has certain research on the aspects of influencing the germination of crop seeds and seedlings, resisting endotoxin and the like at home and abroad, but has less reports on the aspects of inhibiting and eliminating the growth of bacteria and fungal biofilms by chelerythrine.

The antibacterial and acaricidal activity of chelerythrine was reported by the Hu navy et al (the structural-activity relationship between the antibacterial and acaricidal activity of the Hu navy, sanguinarine and chelerythrine [ D ]. northwest university of agriculture and forestry, 2008.). Waqinghui et al reported chelerythrine extraction and bacteriostatic action (Weiqinghui, Chelerythrine extraction and bacteriostatic action research [ D ]. northeast university of forestry, 2013.). In vitro antibacterial activity of chelerythrine ion-pair compounds was studied by kangweisong et al (kangweisong [1], cheng xin [1, 2], et al. Chelerythrine has a certain inhibiting effect on bacteria and fungi, but the application of chelerythrine to the growth of biofilms of candida albicans and staphylococcus aureus is not reported.

Disclosure of Invention

The technical problem to be solved by the invention is to provide the application of chelerythrine in the preparation of the medicines for inhibiting and removing the double species of biofilms of candida albicans and staphylococcus aureus, and the potential biofilm remover is mined from the existing medicinal plant resource library, so that a practical basis is provided for relieving or solving the infection problem caused by the double species of biofilms of candida albicans and staphylococcus aureus, reducing the fatality rate and solving the difficult problem that the double species of biofilms of candida albicans and staphylococcus aureus are difficult to remove.

The invention is realized by the following technical scheme:

use of chelerythrine for inhibiting the growth of biofilm of Candida albicans and Staphylococcus aureus is provided.

Further, the candida albicans is human candida albicans; staphylococcus aureus is a human Staphylococcus aureus.

Further, the concentration that minimally inhibits biofilm formation by both candida albicans and staphylococcus aureus species was determined by crystal violet staining.

Further, the minimum inhibitory concentrations of chelerythrine to candida albicans biofilm and staphylococcus aureus biofilm are 4 mug/mL and 4 mug/mL respectively; the minimum inhibitory concentration on the biofilm of two species of candida albicans and staphylococcus aureus is 6 mug/mL. .

Further, chelerythrine has an in vitro inhibitory effect on the biofilm of two species of candida albicans and staphylococcus aureus, and respectively inhibits the in vitro growth of candida albicans and staphylococcus aureus.

Application of chelerythrine in preparation of medicines for inhibiting and killing Candida albicans and Staphylococcus aureus double species biofilm is provided.

Further, the candida albicans is human candida albicans; staphylococcus aureus is a human Staphylococcus aureus.

A pharmaceutical composition for killing Candida albicans and Staphylococcus aureus double species biofilm comprises chelerythrine with prophylactic or therapeutic effective amount and pharmaceutically acceptable adjuvant or carrier.

Further, killing the candida albicans and staphylococcus aureus dual species biofilm includes interfering with the formation of the candida albicans and staphylococcus aureus dual species biofilm or removing the mature candida albicans and staphylococcus aureus dual species biofilm.

Compared with the prior art, the invention has the following beneficial technical effects:

the chelerythrine has a remarkable inhibiting effect on the formation of the staphylococcus xylosus biofilm, can effectively intervene in the formation of the candida albicans and staphylococcus aureus dual-species biofilm, and can be applied to the preparation of medicines for inhibiting the formation of the candida albicans and staphylococcus aureus, intervening in the formation of the candida albicans and staphylococcus aureus dual-species biofilm and killing mature candida albicans and staphylococcus aureus dual-species biofilm.

Drawings

FIG. 1 shows the intervention effect of chelerythrine on the biofilm of Candida albicans and Staphylococcus aureus at different concentrations;

FIG. 2 shows the structural effect of chelerythrine on the biofilm of Candida albicans and Staphylococcus aureus at different concentrations of drug.

Detailed Description

The present invention will be described in further detail with reference to examples. The examples are only for explaining the contents of the present invention and do not limit the present invention.

1. Material

1.1 test strains

Candida albicans SC5314 was purchased from American Standard culture Collection;

staphylococcus aureus ATCC25923 was purchased from the american standard strain collection center.

1.2 reagents

Dimethyl sulfoxide DMSO purchased from shanghai sigma aldrich trade ltd;

normal saline was purchased from Shanghai Sigma Aldrich trade, Inc.;

methanol purchased from Shanghai Sigma Aldrich trade, Inc.;

yeast extract powder was purchased from Shanghai Sigma Aldrich trade, Inc.;

glucose was purchased from shanghai sigma aldrich trade ltd;

peptone was purchased from shanghai sigma aldrich trade ltd;

glutaraldehyde is available from Shanghai Sigma Aldrich trade, Inc.;

absolute ethanol was purchased from shanghai sigma aldrich trade ltd;

penta-ethyl acetate was purchased from Shanghai Sigma Aldrich trade company, Inc.

1.3 preparation of Primary reagents

YPD liquid medium: 5g of yeast extract powder, 10g of glucose and 10g of peptone were weighed, 500mL of distilled water was added, and autoclave sterilization was performed.

1% DMSO solution: 99mL of water was added to 1mL of 100% DMSO and mixed well.

99% methanol solution: 99mL of 100% methanol was added to 1mL of water and mixed well.

0.1% crystal violet solution: 0.1g of crystal violet is weighed, 100mL of distilled water is added, and the mixture is mixed evenly.

33% glacial acetic acid solution: 33mL of glacial acetic acid was weighed, 67mL of distilled water was added, and the mixture was mixed well.

1.4 Experimental instruments

Autoclave (Tianjin Tester instruments Co., Ltd.)

DH6000A electric constant temperature incubator (Tester instruments Co., Tianjin)

Clean bench (Suzhou Su clean equipment Co., Ltd.)

96-well plate (Denmark Saimer Feishale science Co., Ltd.)

24 orifice plate (Denmark Saimer Feishale science and technology Co., Ltd.)

Electronic balance (Shanghai Jinketianmei Co., Ltd.)

Enzyme marker (Shenzhen Mairui biological medical treatment, Limited)

Field emission scanning electron microscope (FEI Co., Ltd., USA)

Intervention effect of chelerythrine on double-species biofilm of candida albicans and staphylococcus aureus

Chelerythrine is one of the main effective components of the traditional natural medicinal plant, chelidonine. Has antipyretic, analgesic, antiinflammatory, and antitumor effects, and also has antifungal and antibacterial effects. The molecular formula is C₂₁H₁₈NO₄Molecular weight is 348.37, and the molecular structure is as follows:

1. experimental methods

1.1 preparation of the drug

0.01g of chelerythrine is precisely weighed and dissolved in 10mL of 1% DMSO to prepare a drug with the concentration of 1mg/mL as stock solution. 1mL of the drug was taken. The drug was filtered through a 0.22 μm organic phase filter and diluted in 1% DMSO. For use in measuring MIC.

1.2 preparation of bacterial liquid

Candida albicans SC5314 and Staphylococcus aureus ATCC25923 stored at-20 ℃ are taken, operated near an ignited alcohol lamp in a super clean bench, respectively inoculated with inoculating loop dipping bacteria liquid, cultured in a glass test tube containing YPD, and put in a constant temperature incubator at 37 ℃ for 24 hours. After the Candida albicans and the staphylococcus aureus are subcultured, purified and cultured by the same inoculation method, the culture medium is diluted to 1.0 multiplied by 10⁶CFU/mL is ready for use.

1.3 measurement of minimum inhibitory concentration MIC of chelerythrine on Candida albicans and Staphylococcus aureus

Adding the diluted bacteria liquid into a 96-well plate by a liquid transfer device, adding 180 mu L of bacteria liquid into each hole, sucking 20 mu L of diluted chelerythrine medicine with different concentrations, and adding the diluted chelerythrine medicine into the 96-well plate. So that the final system is 200. mu.L. And then respectively preparing a control group which is not added with the medicament and only contains the culture medium and a control group which is only added with the bacteria liquid and is not added with the medicament. Each medicine is made into 3 groups in parallel, and is cultured in a constant temperature incubator at 37 ℃ for 24 hours to observe results, and the hole with the lowest concentration of the aseptically grown medicine is the MIC of chelerythrine on the minimum inhibitory concentration of candida albicans and staphylococcus aureus.

1.4 detection of chelerythrine interference biofilm formation ability under different drug concentrations

1.4.1 establishment of biofilms

Preparing bacterial liquid according to the preparation steps of the bacterial liquid 1.2, and preparing the medicine according to the preparation steps of the medicine. To a 96-well plate, 200. mu.L of the bacterial solution and each concentration of the drug (drug concentrations: MIC, 1/2MIC, 1/4MIC, 1/8MIC, 1/16MIC, respectively) were added. Additional control wells were set for blank (medium only), DMSO (same volume of methanol as drug added) and negative (biofilm growth control without drug added). Each concentration 3 set was in parallel. Culturing in a 37 ℃ incubator for 24 h. Establishes an in vitro Candida albicans and staphylococcus aureus dual-species biofilm model.

1.4.2 Crystal Violet staining

The cultured 96-well cell culture plate was taken out, the bacterial solution was discarded, and the plate was gently washed 3 times with sterile PBS buffer. Fixing with 99% methanol for 30min, air drying, staining with 0.1% crystal violet 200 μ L for 30min, washing, and air drying. Adding 33% glacial acetic acid, and shaking for 30 min. The absorbance was measured at 570nm with a microplate reader.

1.4.3 scanning Electron microscopy

1.4.3.1 establishment of biofilms

Preparing bacterial liquid according to the preparation steps of the bacterial liquid 1.2, and preparing the medicine according to the preparation steps of the medicine. 1.8mL of diluted bacterial solution was added to a 96-well plate, and 200. mu.L of the drug was added. Finally, the final concentration of the drug was 1/2 MIC. A control well (no drug added) was also provided. After the cell slide was sterilized with alcohol, it was placed in a 24-well plate and cultured in a 37 ℃ incubator for 24 hours.

1.4.3.2 scanning Electron microscopy

The cell slide was removed and gently rinsed with sterile PBS to remove free bacteria on the biofilm surface. Adding electron microscope glutaraldehyde, fixing cell biofilm at-4 deg.C in dark for 2 hr, and dehydrating with different gradient of ethanol (30%, 50%, 70%, 90%, 100%). Finally, fixing the mixture for 5 hours in a dark place by using ethyl amyl acetate, and observing the mixture by using a field emission scanning electron microscope. 3 random visual fields are taken for each specimen, and the influence of chelerythrine with different concentrations on the biofilm of candida albicans and staphylococcus aureus is observed.

1.5 statistical data analysis

In the experiment, SPSS 20.0 software is adopted to carry out statistical treatment and analysis on data, the data are represented by +/-SD, and multiple comparison and single-factor analysis of variance are adopted to carry out analysis and comparison on each group of data. P <0.05 was significantly different and P <0.01 was significantly different.

2 results of the experiment

2.1 results of MIC assay of Chelerythrine on Candida albicans SC5314 and Staphylococcus aureus ATCC25923

The dilution method is to determine the antibacterial efficacy of the antibacterial agent by measuring the growth presence or absence of an equal amount of bacterial liquid in the liquid culture medium of the drug with different concentrations and the turbidity of the culture medium after growth, and the minimum concentration of the antibacterial agent for inhibiting the growth of the test bacteria measured by the dilution method is called as the minimum inhibitory concentration. The method can accurately and quantitatively determine the antibacterial activity of the drug to be detected, and is simple, convenient and rapid, so that a 96-well plate microdilution method is adopted to determine the antibacterial activity.

The MIC of chelerythrine to Candida albicans SC5314 is 4 mug/mL, the MIC of Staphylococcus aureus ATCC25923 is 3 mug/mL, and the MIC of the mixed bacterial liquid of Candida albicans SC5314 and Staphylococcus aureus ATCC25923 is 8 mug/mL.

2.2 intervention on biofilm Forming ability at different drug concentrations

The crystal violet staining method can quickly, simply and conveniently measure the intervention effect of chelerythrine on the biofilm of candida albicans and staphylococcus aureus. The strength of the biofilm intervention by the medicament is reflected by the OD value under different medicament concentrations.

As shown in figure 1, on the basis of establishing an in vitro staphylococcus aureus biofilm model, the interference effect of chelerythrine on the candida albicans and staphylococcus aureus double-species biofilm at different drug concentrations is researched by a crystal violet staining method, and the OD value of each drug concentration is measured at the position of 570nm of absorbance by using an enzyme-labeling instrument. The OD value decreased with increasing drug concentration, showing a negative correlation. The OD value at each concentration was significantly different from the blank by SPSS software analysis, with P < 0.05. There was no significant difference between the OD values of the DMSO control and the bacterial liquid control, P > 0.05. The chelerythrine has definite intervention effect on the biological membranes of Candida albicans and Staphylococcus aureus.

2.3 Effect on bacterial Activity in biofilm at different drug concentrations

The field emission scanning electron microscope is an important method for observing the biofilm, and can visually observe the form of the biofilm, determine the thickness of the biofilm and clearly observe the distribution of the biofilm and the form of bacteria wrapped by the biofilm without destroying the integrity of the biofilm.

As shown in fig. 2, the effect of chelerythrine at various concentrations on the biofilm activity of candida albicans and staphylococcus aureus was observed by field emission scanning electron microscopy. Biofilm formation was thicker without dosing, as observed by field emission scanning electron microscopy. When the chelerythrine concentration reaches 1/8MIC, the biofilm becomes thinner obviously, and the bacterial activity is in a descending trend. When the chelerythrine concentration reaches 1/4MIC, the biomembrane is obviously inhibited and eliminated, and the bacterial density is in a descending trend. The results show that under the action of the medicine, the living bacteria in the biofilm are inhibited, and the chelerythrine can permeate the biofilm to act inside, namely the chelerythrine has good interference effect on the formation of the candida albicans and staphylococcus aureus two-species biofilm and has obvious killing effect on the mature candida albicans and staphylococcus aureus two-species biofilm.

In conclusion, chelerythrine has an inhibiting effect on candida albicans and staphylococcus aureus and a good killing effect on biological envelope. Therefore, the chelerythrine can be used for preparing the medicine for inhibiting the formation of the two-species biofilm of the candida albicans and the staphylococcus aureus and killing mature two-species biofilm of the candida albicans and the staphylococcus aureus.

A pharmaceutical composition for inhibiting biofilm of Candida albicans and Staphylococcus aureus, comprising: chelerythrine with effective dose for preventing or treating and pharmaceutically acceptable auxiliary materials or carriers.

The carrier or the auxiliary material refers to the conventional carrier or auxiliary material in the pharmaceutical field, such as: diluents, disintegrants, lubricants, excipients, binders, glidants, fillers, surfactants, and the like; in addition, other adjuvants such as flavoring agents and sweeteners may also be added to the composition.

The diluent may be one or more ingredients that increase the weight and volume of the tablet; common diluents include lactose, starch, pregelatinized starch, microcrystalline cellulose, sorbitol, mannitol, and inorganic calcium salts. The most common of them are lactose, starch, microcrystalline cellulose.

The disintegrating agent can be one or a mixture of more of crosslinked polyvinylpyrrolidone (with the total weight ratio of 2-6%), crosslinked sodium carboxymethylcellulose (with the total weight ratio of 2-6%), alginic acid (with the total weight ratio of 2-5%) and microcrystalline cellulose (with the total weight ratio of 5-15%). Wherein the ratio of the crosslinked polyvinylpyrrolidone to the total weight is preferably 2-7%, and the ratio of the crosslinked sodium carboxymethylcellulose to the total weight is preferably 2-6%. The cross-linked polyvinylpyrrolidone is the best (the ratio of the cross-linked polyvinylpyrrolidone to the total weight is 2-6%).

The lubricant comprises one or more of stearic acid, sodium stearate, magnesium stearate, calcium stearate, polyethylene glycol, talcum powder and hydrogenated vegetable oil. Magnesium stearate is most preferred. The dosage range of the lubricant is 0.10-1% of the total weight, the general dosage is 0.25-0.75% of the total weight, and the optimal dosage is 0.5-0.7% of the total weight.

The binder may be one or more ingredients that facilitate granulation. The adhesive may be starch slurry (10-30% by weight of the adhesive), hydroxypropyl methylcellulose (2-5% by weight of the adhesive), polyvinylpyrrolidone (2-20% by weight of the adhesive), and ethanol aqueous solution of polyvinylpyrrolidone, preferably 50% ethanol aqueous solution of polyvinylpyrrolidone.

The glidant can be one or more of micropowder silica gel, talcum powder and magnesium trisilicate.

The surfactant may be one or more components that improve wetting and increase drug dissolution. Sodium dodecyl sulfate is commonly used (the common range of the ratio to the total weight is 0.2-6%).