Use of coenzyme Q10 preparation for treating and preventing epidermolysis bullosa
阅读说明：本技术 辅酶q10制剂在治疗和预防大疱性表皮松解症中的用途 (Use of coenzyme Q10 preparation for treating and preventing epidermolysis bullosa ) 是由 N·R·纳莱恩 R·萨兰加拉詹 M·A·凯比施 于 2018-05-16 设计创作，主要内容包括：本发明部分地涉及在有需要的受试者中治疗大疱性表皮松解症(EB)的方法,其包括向该受试者局部施用包含治疗有效量的辅酶Q10(CoQ10)的药物组合物。(The present invention relates in part to a method of treating Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of coenzyme Q10(CoQ 10).)
1. A method of treating Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of coenzyme Q10(CoQ 10).
2. The method of claim 1, wherein the epidermolysis bullosa is epidermolysis bullosa simplex, epidermolysis bullosa junctional, dystrophic epidermolysis bullosa, or Kindler's syndrome.
3. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of about one to about twelve weeks.
4. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 14 times per week for the duration of treatment.
5. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 7 times per week for the duration of treatment.
6. The method of any one of claims 1-5, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice a day for the duration of treatment.
7. The method of any one of claims 1-5, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about once per day for the duration of treatment.
8. The method of any one of claims 1-5, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once every two days for the duration of treatment.
9. The method of any one of claims 1-5, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice per week for the duration of treatment.
10. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about two weeks.
11. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about three weeks.
12. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about four weeks.
13. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about five weeks.
14. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about six weeks.
15. The method of any one of claims 1-9, wherein administration of the pharmaceutical composition comprising CoQ10 to the site comprising at least one blister and/or wound for a treatment duration of about four weeks results in at least a 70% reduction in the size of the site comprising at least one blister and/or wound.
16. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 comprises about 1% to about 5% (w/w) CoQ 10.
17. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 comprises about 3% (w/w) CoQ 10.
18. The method of any one of the preceding claims, wherein administering the pharmaceutical composition comprising CoQ10 to the subject provides one or more beneficial effects to the subject.
19. The method of claim 18, wherein the one or more benefits are selected from the group consisting of:
a. reducing pain associated with EB;
b. reducing inflammation associated with EB;
c. reducing the size of blisters and/or wounds associated with an EB;
d. reducing the number of blisters and/or wounds associated with an EB;
e. increasing the rate of healing of one or more blisters and/or wounds associated with EB;
f. increasing the structural integrity of the skin of a subject with EB;
g. reducing the number of skin infections associated with EBs;
h. increasing wound closure of wounds associated with EB;
i. increasing re-epithelialization of wounds associated with EB;
j. increasing granulation of wounds associated with EB;
k. reducing epidermal gap distance of blisters and/or wounds associated with an EB;
reducing time to blister and/or wound healing associated with EB;
reducing the amount of concomitant medication administered to the subject to treat EB in the subject;
n. reducing scar formation associated with EB;
increasing keratinocyte production in the skin of the subject; and
increasing production of fibroblasts in the skin of the subject.
20. A method of treating Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of CoQ10, wherein treatment of the subject results in a reduction in the size of one or more blisters and/or wounds of at least about 70% after administration of an effective amount of CoQ10 for a treatment duration of about four weeks.
21. The method of claim 20, wherein the pharmaceutical composition comprising CoQ10 contains about 1% to about 5% (w/w) CoQ 10.
22. The method of claim 20 or 21, wherein the pharmaceutical composition comprising CoQ10 contains about 3% (w/w) CoQ 10.
23. The method of any one of claims 20-22, wherein administration of the CoQ10 to the subject provides a beneficial effect to the subject.
24. The method of claim 23, wherein the beneficial effect is one or more selected from the group of:
a. reducing pain associated with EB;
b. reducing inflammation associated with EB;
c. reducing the size of blisters and/or wounds associated with an EB;
d. reducing the number of blisters and/or wounds associated with an EB;
e. increasing the rate of healing of one or more blisters and/or wounds associated with EB;
f. increasing the structural integrity of the skin of a subject with EB;
g. reducing the number of skin infections associated with EBs;
h. increasing wound closure of wounds associated with EB;
i. increasing re-epithelialization of wounds associated with EB;
j. increasing granulation of wounds associated with EB;
k. reducing epidermal gap distance of blisters and/or wounds associated with an EB;
reducing time to blister and/or wound healing associated with EB;
reducing the amount of concomitant medication administered to the subject to treat EB in the subject;
n. reducing scar formation associated with EB;
increasing keratinocyte production in the skin of the subject; and
increasing production of fibroblasts in the skin of the subject.
25. A method of treating a wound associated with Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of CoQ 10.
26. The method of claim 25, wherein the epidermolysis bullosa is epidermolysis bullosa simplex, epidermolysis bullosa junctional, dystrophic epidermolysis bullosa, or Kindler's syndrome.
27. The method of any one of claims 25 or 26, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of about one week to about twelve weeks.
28. The method of any one of claims 24-27, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 14 times per week for the duration of treatment.
29. The method of any one of claims 24-28, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 7 times per week for the duration of treatment.
30. The method of any one of claims 24-27, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about twice a day for the duration of treatment.
31. The method of any one of claims 24-28, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once a day for the duration of treatment.
32. The method of any one of claims 24-28, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once every two days for the duration of treatment.
33. The method of any one of claims 24-28, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice per week for the duration of treatment.
34. The method of any one of claims 24-33, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about two weeks.
35. The method of any one of claims 24-34, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about three weeks.
36. The method of any one of claims 24-35, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about four weeks.
37. The method of any one of claims 24-36, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about five weeks.
38. The method of any one of claims 24-37, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about six weeks.
39. The method of any one of claims 24-36, wherein administration of the pharmaceutical composition comprising CoQ10 to the site comprising at least one blister and/or wound for a treatment duration of about four weeks results in at least a 70% reduction in the size of the site comprising at least one blister and/or wound.
40. The method of any one of claims 24-39, wherein the pharmaceutical composition comprising CoQ10 contains about 1% to about 5% (w/w) CoQ 10.
41. The method of claim 40, wherein the pharmaceutical composition comprising CoQ10 contains about 3% (w/w) CoQ 10.
42. The method of any one of claims 24-41, wherein administering the pharmaceutical composition comprising CoQ10 to the subject provides one or more beneficial effects to the subject.
43. The method of claim 42, wherein said one or more beneficial effects are selected from the group consisting of:
a. reducing pain associated with EB;
b. reducing inflammation associated with EB;
c. reducing the size of blisters and/or wounds associated with an EB;
d. reducing the number of blisters and/or wounds associated with an EB;
e. increasing the rate of healing of one or more blisters and/or wounds associated with EB;
f. increasing the structural integrity of the skin of a subject with EB;
g. reducing the number of skin infections associated with EBs;
h. increasing wound closure of wounds associated with EB;
i. increasing re-epithelialization of wounds associated with EB;
j. increasing granulation of wounds associated with EB;
k. reducing epidermal gap distance of blisters and/or wounds associated with an EB;
reducing time to blister and/or wound healing associated with EB;
reducing the amount of concomitant medication administered to the subject to treat EB in the subject;
n. reducing scar formation associated with EB;
increasing keratinocyte production in the skin of the subject; and
increasing production of fibroblasts in the skin of the subject.
44. A method of improving the structural integrity of the skin of a subject with epidermolysis bullosa comprising topically administering to the skin of the subject a pharmaceutical composition comprising a therapeutically effective amount of CoQ 10.
45. The method of claim 44, wherein the poor structural integrity of the skin of the subject with EB is the result of a defect or deficiency in one or more structural and/or functional proteins.
46. The method of claim 45, wherein the one or more structural and/or functional proteins are selected from the group consisting of keratin, collagen, lectin, annexin, vimentin, fibrin, integrin, and laminin.
47. The method of claim 46, wherein the keratin is selected from the group consisting of keratin 5(KRT5), keratin 13(KRT13), keratin 14(KRT14), and keratin 17(KRT 17).
48. The method according to claim 46, wherein the collagen is selected from collagen XVII or collagen VII.
49. The method of claim 46, wherein the laminin is laminin 332.
50. The method of claim 46, wherein the integrin is α 6 β 4 integrin.
51. The method according to claim 45 or 46, wherein the protein is selected from the group consisting of transaldolase 1, NM23 protein, heat shock 27kDa protein 1, keratin 14, keratin 13, proteasome β 7, proteasome activator subunit 3, and rhoGDP dissociation inhibitor α.
52. The method according to claim 45 or 46, wherein the protein is selected from the group consisting of V-akt murine thymoma virus oncogene homolog 1(AKTl), BCL 2-related oncogene 4(BAG4), BCL 2-related protein X (BAX), BCL 2-like 1(BCL2L1), BCL 2/adenovirus E1B 19kDa interacting protein 3(BNTP3), caspase recruitment domain family member 6(CARD6), caspase 6, apoptosis-related cysteine peptidase (CASP6), caspase 7, apoptosis-related cysteine peptidase (CASP7), growth arrest and DNA damage inducible, CAR α (GADD45A), tumor protein p53(TP53), and tumor protein p73(TP 73).
53. The method of any one of claims 45-52, wherein the defect or deficiency in one or more structural and/or functional proteins is within the epidermis.
54. The method of any one of claims 45-52, wherein the defect or deficiency in the one or more structural and/or functional proteins is within a lamina lucida.
55. The method of any one of claims 45-52, wherein the defect or deficiency in one or more structural and/or functional proteins is in the dense underboard band.
56. The method of any one of claims 45-52, wherein the defect or deficiency in the one or more structural and/or functional proteins is within more than one of the epidermis, the transparent layer, and the lower band of dense plates.
57. The method of claim 53, wherein the EB is epidermolysis bullosa simplex.
58. The method of claim 54, wherein the EB is junctional epidermolysis bullosa.
59. The method of claim 55, wherein the EB is dystrophic epidermolysis bullosa.
60. The method of claim 56, wherein the EB is Kindler syndrome.
61. The method of any one of claims 45-60, wherein the defect or deficiency in one or more structural and/or functional proteins is one or more of insufficient protein activity, insufficient protein expression, and protein mutation.
62. The method of claim 44, wherein the structural integrity of the skin is assessed by histological examination of a skin biopsy from the subject, Transmission Electron Microscopy (TEM), or immunofluorescence staining.
63. The method of any one of claims 44-62, wherein the improvement in the structural integrity of skin is determined by providing one or more beneficial effects to the subject with EB following treatment with an effective amount of CoQ 10.
64. The method of claim 63, wherein said one or more beneficial effects are selected from the group consisting of:
a. reducing pain associated with EB;
b. reducing inflammation associated with EB;
c. reducing the size of blisters and/or wounds associated with an EB;
d. reducing the number of blisters and/or wounds associated with an EB;
e. increasing the rate of healing of one or more blisters and/or wounds associated with EB;
f. increasing the structural integrity of the skin of a subject with EB;
g. reducing the number of skin infections associated with EBs;
h. increasing wound closure of wounds associated with EB;
i. increasing re-epithelialization of wounds associated with EB;
j. increasing granulation of wounds associated with EB;
k. reducing epidermal gap distance of blisters and/or wounds associated with an EB;
reducing time to blister and/or wound healing associated with EB;
reducing the amount of concomitant medication administered to the subject to treat EB in the subject;
n. reducing scar formation associated with EB;
increasing keratinocyte production in the skin of the subject; and
increasing production of fibroblasts in the skin of the subject.
65. The method of any one of claims 44-64, wherein the improvement in the structural integrity of skin is determined using an attracting blister test on the skin of a subject with EB following treatment with an effective amount of CoQ 10.
66. The method of claim 65, wherein an increase in time to blister formation in the subject's skin treated with CoQ10 is indicative of an improvement in the structural integrity of the subject's skin.
67. The method of any one of claims 44-66, wherein the pharmaceutical composition comprising CoQ10 contains about 1% to about 5% (w/w) CoQ 10.
68. The method of any one of claims 44-67, wherein the pharmaceutical composition comprising CoQ10 contains about 3% (w/w) CoQ 10.
69. A method of increasing the rate of healing of a skin blister and/or wound in a subject with EB comprising topically applying to the skin blister and/or wound a pharmaceutical composition comprising a therapeutically effective amount of coenzyme Q10(CoQ 10).
70. The method of claim 69, wherein said rate of healing is determined from the rate of epithelial regeneration of a skin blister and/or wound treated with said CoQ10 composition compared to the rate of epithelial regeneration of an untreated blister and/or wound.
71. The method of claim 69, wherein the rate of healing is determined by a decrease in epidermal gap distance of a skin blister and/or wound treated with the CoQ10 composition as compared to the epidermal gap distance of an untreated blister and/or wound.
72. The method of any of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is in the form of CoQ10 cream at 0.01mg/cm2To 5mg/cm2The dose of CoQ10 in (a).
73. The method of any one of the preceding claims, wherein the pharmaceutical composition comprising CoQ10 is administered with a second composition comprising an additional agent.
74. The method of any one of the preceding claims, wherein the pharmaceutical composition further comprises an additional agent.
75. A method of treating or preventing squamous cell carcinoma in a subject with epidermolysis bullosa comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of CoQ 10.
76. The method of claim 75, wherein the squamous cell carcinoma is a cutaneous squamous cell carcinoma.
77. The method of claim 75 or 76, wherein the epidermolysis bullosa is junctional epidermolysis bullosa, dystrophic epidermolysis bullosa, or Kindler syndrome.
78. The method of any one of claims 75-77, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of about one week to about twelve weeks.
79. The method of any one of claims 75-78, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 14 times per week for the duration of treatment.
80. The method of any one of claims 75-79, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 7 times per week for the duration of treatment.
81. The method of any one of claims 75-78, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice a day for the duration of treatment.
82. The method of any one of claims 75-79, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once per day for the duration of treatment.
83. The method of any one of claims 75-79, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once every two days for the duration of treatment.
84. The method of any one of claims 75-79, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice per week for the duration of treatment.
85. The method of any one of claims 75-84, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about two weeks.
86. The method of any one of claims 75-85, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about three weeks.
87. The method of any one of claims 75-86, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about four weeks.
88. The method of any one of claims 75-87, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about five weeks.
89. The method of any one of claims 75-88, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about six weeks.
90. The method of any one of claims 75-89, wherein the pharmaceutical composition comprising CoQ10 contains about 1% to about 5% (w/w) CoQ 10.
91. The method of any one of claims 75-90, wherein the pharmaceutical composition comprising CoQ10 contains about 3% (w/w) CoQ 10.
92. The method of any one of claims 75-91Method, wherein the pharmaceutical composition comprising CoQ10 is in the form of a CoQ10 cream at 0.01mg/cm2To 5mg/cm2The dose of CoQ10 in (a).
93. The method of any one of claims 75-92, wherein the pharmaceutical composition is applied to intact skin and skin having one or more blisters and/or wounds.
94. The method of any one of claims 75-93, wherein the pharmaceutical composition is applied to intact skin.
95. The method of any one of claims 75-92, wherein the pharmaceutical composition is applied to skin having one or more blisters and/or wounds.
96. The method of any one of claims 75-95, wherein treatment of the subject results in a delay in the onset of SCC as compared to the average age of onset of SCC in the form of EB that the subject has.
97. The method of any one of claims 75-96, wherein the pharmaceutical composition comprising CoQ10 is administered with a second composition comprising an additional agent.
98. The method of any one of claims 75-96, wherein the pharmaceutical composition further comprises an additional agent.
99. The method of claim 97 or 98, wherein the additional agent is a chemotherapeutic agent.
100. The method of claim 97, wherein the chemotherapeutic agent is cisplatin, doxorubicin, 5-fluorouracil, capecitabine, topotecan, or etoposide.
101. The method of claim 98, wherein the additional agent is 5-fluorouracil.
102. The method of claim 97 or 98, wherein the additional agent is diclofenac, imiquimod, or butanediol formate.
103. The method of claim 97 or 98, wherein said additional agent is a drug used in photodynamic therapy (PDT).
104. A topical pharmaceutical composition comprising a therapeutically effective amount of CoQ10 for use in treating Epidermolysis Bullosa (EB) in a subject in need thereof.
105. The composition of claim 104, for use in the treatment of EB, wherein treatment of EB comprises treatment of one or more blisters and/or wounds associated with EB.
106. The composition of claim 104 or 105, for use in treating EB, wherein treatment of EB comprises improving the structural integrity of skin in a subject with EB.
107. The composition of any one of claims 104-106 for use in treating EB, wherein the EB is epidermolysis bullosa simplex, epidermolysis bullosa junctional, epidermolysis bullosa dystrophia or Kindler's syndrome.
108. The composition of any one of claims 104-107, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of about one week to about twelve weeks.
109. The composition of any one of claims 104-108, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 14 times per week for the duration of treatment.
110. The composition of any one of claims 104-109, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 7 times per week for the duration of treatment.
111. The composition of any one of claims 104-109, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice a day for the duration of treatment.
112. The composition of any one of claims 104-110, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once a day for the duration of treatment.
113. The composition of any one of claims 104-110, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once every two days for the duration of treatment.
114. The composition of any one of claims 104-110, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice per week for the duration of treatment.
115. The composition of any one of claims 104-114, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about two weeks.
116. The composition of any one of claims 104-115, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about three weeks.
117. The composition of any one of claims 104-116, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about four weeks.
118. The composition of any one of claims 104-117, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about five weeks.
119. The composition of any one of claims 104-118, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about six weeks.
120. The composition of any one of claims 104-117, wherein administration of the pharmaceutical composition comprising CoQ10 to a site comprising at least one blister and/or wound for a treatment duration of about four weeks results in at least a 70% reduction in the size of the site comprising at least one blister and/or wound.
121. The composition of any one of claims 104-120, wherein the pharmaceutical composition comprising CoQ10 comprises about 1% to about 5% (w/w) CoQ 10.
122. The composition of any one of claims 104-121, wherein the pharmaceutical composition comprising CoQ10 comprises about 3% (w/w) CoQ 10.
123. The composition of any one of claims 104-122, wherein administration of the pharmaceutical composition comprising CoQ10 to the subject provides one or more beneficial effects to the subject.
124. The composition according to claim 123, wherein said one or more benefits are selected from the group consisting of:
a. reducing pain associated with EB;
b. reducing inflammation associated with EB;
c. reducing the size of blisters and/or wounds associated with an EB;
d. reducing the number of blisters and/or wounds associated with an EB;
e. increasing the rate of healing of one or more blisters and/or wounds associated with EB;
f. increasing the structural integrity of the skin of a subject with EB;
g. reducing the number of skin infections associated with EBs;
h. increasing wound closure of wounds associated with EB;
i. increasing re-epithelialization of wounds associated with EB;
j. increasing granulation of wounds associated with EB;
k. reducing epidermal gap distance of blisters and/or wounds associated with an EB;
reducing time to blister and/or wound healing associated with EB;
reducing the amount of concomitant medication administered to the subject to treat EB in the subject;
n. reducing scar formation associated with EB;
increasing keratinocyte production in the skin of the subject; and
increasing production of fibroblasts in the skin of the subject.
125. A topical pharmaceutical composition comprising a therapeutically effective amount of CoQ10 for use in treating Squamous Cell Carcinoma (SCC) associated with Epidermolysis Bullosa (EB) in a subject in need thereof.
126. The pharmaceutical composition of claim 125, for use in the treatment of SCC, wherein the SCC is cutaneous squamous cell carcinoma.
127. The method of claim 125 or 126, wherein the EB is junctional epidermolysis bullosa, dystrophic epidermolysis bullosa, or Kindler's syndrome.
128. The pharmaceutical composition of any one of claims 125-127 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of about one week to about twelve weeks.
129. The pharmaceutical composition of any one of claims 125-128 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 14 times per week for the duration of the treatment.
130. The pharmaceutical composition of any one of claims 125-128 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject from 1 to about 7 times per week for the duration of the treatment.
131. The pharmaceutical composition of any one of claims 125-129 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice a day for the duration of the treatment.
132. The pharmaceutical composition of any one of claims 125-130 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once a day for the duration of the treatment.
133. The pharmaceutical composition of any one of claims 125-130 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject about once every two days for the duration of the treatment.
134. The pharmaceutical composition of any one of claims 125-130 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered to the subject at about twice a week for the duration of the treatment.
135. The pharmaceutical composition of any one of claims 125-134 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about two weeks.
136. The pharmaceutical composition of any one of claims 125-135 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about three weeks.
137. The pharmaceutical composition of any one of claims 125-136 for use in the treatment of SCC wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about four weeks.
138. The pharmaceutical composition of any one of claims 125-137 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about five weeks.
139. The pharmaceutical composition of any one of claims 125-138 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered for a treatment duration of at least about six weeks.
140. The pharmaceutical composition of any one of claims 125-139 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 contains about 1% to about 5% (w/w) CoQ 10.
141. The pharmaceutical composition of any one of claims 125-140 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 contains about 3% (w/w) CoQ 10.
142. The pharmaceutical composition of any one of claims 125-141 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is in the form of CoQ10 cream at 0.01mg/cm2To 5mg/cm2Dose of CoQ 10.
143. The pharmaceutical composition of any one of claims 125-142 for use in the treatment of SCC, wherein the pharmaceutical composition is applied to intact skin and skin having one or more blisters and/or wounds.
144. The pharmaceutical composition of any one of claims 125-142 for use in the treatment of SCC, wherein the pharmaceutical composition is administered to intact skin.
145. The pharmaceutical composition of any one of claims 125-142 for use in the treatment of SCC, wherein the pharmaceutical composition is administered to skin having one or more blisters and/or wounds.
146. The pharmaceutical composition of any one of claims 125-145, for use in the treatment or prevention of SCC, wherein the treatment or prevention of the subject results in a delay in the onset of SCC as compared to the average age of onset of SCC in the form of EB that the subject has.
147. The pharmaceutical composition of any one of claims 125-146 for use in the treatment of SCC, wherein the pharmaceutical composition comprising CoQ10 is administered with a second composition comprising an additional agent.
148. The pharmaceutical composition of any one of claims 125-146 for use in the treatment of SCC, wherein the pharmaceutical composition further comprises an additional agent.
149. The method of claim 147 or 148, wherein the additional agent is a chemotherapeutic agent.
150. The method of claim 147, wherein the chemotherapeutic agent is cisplatin, doxorubicin, 5-fluorouracil, capecitabine, topotecan, or etoposide.
151. The method of claim 148, wherein the additional agent is 5-fluorouracil.
152. The method of claim 147 or 148, wherein the additional agent is diclofenac, imiquimod, or butanediol formate.
153. The method of claim 147 or 148, wherein said additional agent is an agent used in photodynamic therapy (PDT).
Background
According to the institute of dystrophic epidermolysis bullosa (DebRA), 1 out of 50,000 infants born is affected by Epidermolysis Bullosa (EB). It is found in all ethnic and ethnic groups as well as in men and women around the world. EB comprises a genetically determined group of skin-debilitating diseases characterized by blistering or tearing of the skin and mucous membranes following mild mechanical injury. Traditionally, genetic EBs have been considered to be of three major classes, namely simple EBs, junctional EBs and dystrophic EBs, based on the ultrastructural level of skin division and blisters (Pearson, r.w., 1988). In 2007, the third international conference on EB diagnosis and classification held in Vienna, Austria. Based on the results of this meeting, EBs are now divided into 4 main types. Mixed type (Kindler syndrome) is the fourth largest type. The availability of monoclonal and polyclonal antibodies and the development of molecular diagnostic techniques have divided EBs into at least 30 distinct subtypes (Fine JD 2010).
One of the hallmarks of these diseases is the formation of blisters or wounds caused by mild mechanical trauma, friction or heat, the severity of the disease depending on the type of EB present. Blisters and/or wound healing in EB patients is prone to delay and susceptibility to infection. All forms of simple eb (ebs) have fragile sites that are prone to rupture and form lesions in the epidermis (Hanna, Silverman, Boxall, krafcik, 1983), usually beginning with the destruction of basal keratinocytes. EBS is usually dominant inherited and is involved in genetic diseases encoding keratin 5, 14 and clathrin. In the rare EBS forms, there may also be the possibility of lectin mutations and muscular dystrophy. In contrast, the connective and dystrophic forms of EB are the result of structural disruption in or near the basal membrane region. In interfacial eb (jeb), skin breakdown occurs uniformly in the narrow electron sparse upper transparent layer at the dermal-epidermal junction. JEB is a recessive genetic disorder involving many genes involved in the composition between the epidermis and dermis, such as laminin 332, clathrin, and a6b 4-integrin. In dystrophic eb (deb), disruption always occurs between the levels of the dense layer, the lower half of the electron concentration at the epidermis-epidermis junction (the upper dermis). (Fine JD., Schachner LA, 1995). Kindler syndrome accounts for approximately 1% of EB patients, involving all layers of extremely fragile skin.
The EB phenotype is further sub-classified according to the degree of skin involvement (both local and systemic), the specific nature of the lesion area distribution, the type of morphological lesions present on the skin, the degree (if any) and diversity of extradermal disease activity, and the mode of inheritance (autosomal dominant inheritance, autosomal recessive inheritance). All forms of EB are characterized by recurrent blisters and/or wounds throughout life, with persistent infection. Blisters may form anywhere on the surface of the skin, including within the oral cavity, the surface of the eye, the respiratory tract, the gastrointestinal tract, and/or the urogenital tract. In addition to chronic blisters and wounds, scars and musculoskeletal deformities may also occur.
Some forms of EB are associated with normal human life span. However, several forms of severe EB lead to severe morbidity and to a significant increase in mortality as a direct or indirect result of EB. Many EB patients become anemic due to chronic blood loss from the wound, inadequate nutrient intake, iron malabsorption, and chronic inflammation inhibiting the bone marrow. Other patients suffer from selenium and carnitine or vitamin D deficiency, which predisposes them to osteoporosis and/or cardiomyopathy. Death usually occurs in the first three decades of severely affected patients. The reason is repeated infection of anemia patients with malnutrition. Another serious complication of malnutrition and borderline forms of EB is the development of invasive squamous cell carcinoma in adulthood. Most patients die from a wide range of metastatic diseases regardless of the time and strength of diagnosis and treatment of the primary tumor. Trachea-throat obstruction is also a risk for a small number of patients with various forms of borderline EB.
Skin involvement (wounds) ranges from blisters in the extremities to a more extensive wound throughout the body. The wound may occur as a single lesion or may appear in clusters, depending on the site and/or cause of the injury. If not treated in time, the focus of EB patients tends to enlarge and fail to heal. Typical wound healing (in a population not affected by EB) is a delicate series of events that are histologically predictable, involving immune cells, platelets, keratinocytes, fibroblasts and macrophages, which play a crucial role (Demidova-Rice et al, 2012). There are two major types of DEBs, one dominant and the other recessive, that are involved in defects in type VII collagen. Genetic aberrations in the skin of EB patients can lead to life-long skin problems that are never addressed and have a significant adverse impact on quality of life.
In contrast to normal wound healing, chronic wounds, such as those observed in EB patients, exhibit a sustained severe inflammatory phase without progressing to the healing phase (Schober-Flores c., 2003). Due to the long-term, chronic nature of these wounds, they are prone to persistent infection. EB patients suffer from pain and discomfort, struggle with recurrent skin infections, have severely limited lifestyle choices, and often suffer from anemia, malnutrition, constipation, osteoporosis, cardiomyopathy, cancer, and other diseases. Standard-of-care wound therapy is not effective in treating patients with EB because their skin lacks expression of appropriate structural and functional proteins that are used to prevent or repair damage from environmental factors (e.g., heat, moisture) and/or mechanical stresses (e.g., friction, trauma) and are unable to cure EB due to genetic mutations in the patient's skin.
There is no proven specific treatment for any form of EB, and the primary basis for clinical management is wound care, pain management and avoidance of causative factors. Currently, treatment of inherited EBs is essentially supportive. Good topical treatment is of vital importance, mainly including sterile dressings and topical antibiotics. Standards of care include the use of antibiotics in creams or ointments covered with petrolatum-coated gauze or non-stick synthetic dressings. The use of thick tubular gauze protects vulnerable areas from repeated injury. Such dressings are typically replaced daily. When mild infections occur, intermittent use of systemic antibiotics is required.
Regardless of the type of EB diagnosis and the form of the lesion, proper and adequate wound care remains the cornerstone of effective treatment. Although a variety of wound care products are available for wounds in the skin that heal normally, their use in EBs is extremely limited in the clinical setting.
Thus, there remains a strong need in the art for improved treatment options for various forms of EB.
Disclosure of Invention
The present invention is based, at least in part, on the surprising discovery that topically applied coenzyme Q10(CoQ10) can be used to treat EB patients. In addition, topically applied CoQ10 can structurally and functionally improve the skin integrity and healing of skin wounds in EB patients. Without being bound by theory, the present inventors report the surprising and unexpected discovery that topical administration of CoQ10 can promote the transition from the inflammatory phase of healing (where EB blisters and/or wounds generally stagnate) to the proliferative and remodeling stages, allowing for the elimination of blisters and/or wounds. The present invention represents a novel therapy for treating EB patients.
In one embodiment of the invention, topical CoQ10 may be used to alter expression of missing structural and/or functional proteins in EB patients, which correction may improve the structural integrity of the skin.
Another embodiment of the invention is a method of treating Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of CoQ10, wherein treatment of the subject results in a reduction in the size of one or more blisters and/or wounds of at least about 70% after administration of an effective amount of CoQ10 for a treatment duration of about four weeks.
Another embodiment of the invention is a method of treating a wound associated with Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of
Another embodiment of the invention is a method of improving the structural integrity of the skin of a subject with epidermolysis bullosa comprising topically administering to the skin of the subject a pharmaceutical composition comprising a therapeutically effective amount of
One embodiment of the invention is a method of increasing the rate of healing of a skin blister and/or wound in a subject with EB comprising topically applying to the skin blister and/or wound a pharmaceutical composition comprising a therapeutically effective amount of coenzyme Q10(CoQ 10).
In another embodiment, the invention is a method of treating or preventing squamous cell carcinoma in a subject with epidermolysis bullosa comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of
In any of the above embodiments, the epidermolysis bullosa is simple epidermolysis bullosa, junctional epidermolysis bullosa, dystrophic epidermolysis bullosa, or Kindler's syndrome.
In any of the above embodiments, the pharmaceutical composition comprising CoQ10 is applied to a blister and/or wound on the skin. In any of the embodiments above, the pharmaceutical composition comprising CoQ10 is applied to intact skin. In another embodiment, the topical pharmaceutical composition is a formulation selected from the group consisting of an ointment, a cream, an emulsion, a lotion, and a gel. In one embodiment, the topical pharmaceutical composition is in the form of a cream. In one aspect of this embodiment, the topical pharmaceutical composition comprises from about 1% to about 5% (w/w)
In any of the above embodiments, topical administration of a CoQ10 pharmaceutical composition of the invention provides one or more beneficial effects to a subject suffering from EB following treatment with an effective amount of
In one embodiment, administration of a composition comprising a therapeutically effective amount of CoQ10 reduces the healing time of the blister and/or wound compared to an untreated blister and/or wound. In another embodiment, administration of a composition comprising a therapeutically effective amount of CoQ10 improves the quality of healing with Epidermolysis Bullosa Disease Activity and Scarring Index (EBDASI) compared to untreated blisters and/or wounds. In one embodiment, administration of a composition comprising a therapeutically effective amount of CoQ10 increases the resistance to mechanical damage to the skin compared to untreated skin. In one embodiment, administration of a composition comprising a therapeutically effective amount of CoQ10 reduces the formation of blisters and/or wounds in the skin compared to untreated skin. In another embodiment, a composition comprising CoQ10 is applied to a blister and/or wound on the skin. In another embodiment, a composition comprising CoQ10 is applied to intact skin.
In another aspect, the invention features a method of promoting healing of a skin blister and/or wound in a subject with epidermolysis bullosa, the method including topically applying to the blister and/or wound a composition including a therapeutically effective amount of coenzyme Q10(CoQ10), thereby promoting healing of the skin blister and/or wound in the subject. In one embodiment, the rate of re-epithelialization of blisters and/or wounds treated with CoQ10 compositions is increased as compared to the rate of re-epithelialization of untreated blisters and/or wounds. In one embodiment, the epidermal gap distance of the blister and/or wound is reduced as compared to the epidermal gap distance of an untreated blister and/or wound. In one embodiment, the quality of epidermal integrity of blisters and/or wounds treated with a CoQ10 composition is improved as compared to the quality of epidermal integrity of untreated blisters and/or wounds. In another embodiment, histological examination of the bleb top (blister roof) is used to assess the quality of epidermal integrity. In one embodiment, the expression level of one or more structural proteins in a blister and/or wound treated with a CoQ10 composition is modulated as compared to the expression level of one or more structural proteins in an untreated blister and/or wound. In another embodiment, the one or more structural proteins are selected from the group consisting of keratin, collagen, lectin, annexin, vimentin, filaggrin, and laminin. In another embodiment, the keratin is selected from the group consisting of keratin 13(KRT13), keratin 14(KRT14), and keratin 17(KRT 17). In another embodiment, the protein is one or more proteins selected from the group consisting of keratin 5, keratin 14, lectins, laminin 332, a6b 4-integrin, and type VII collagen.
In another aspect, the invention features a method of treating a subject with epidermolysis bullosa, comprising administering to the subject a composition comprising a therapeutically effective amount of CoQ10, thereby treating the subject. In one embodiment, the subject is identified as having an alteration in the expression of one or more structural proteins in the skin. In another embodiment, the one or more structural proteins are selected from the group consisting of keratin, collagen, lectin, annexin, vimentin, filaggrin, and laminin. In another embodiment, the keratin is selected from the group consisting of keratin 13(KRT13), keratin 14(KRT14), and keratin 17(KRT 17). In another aspect of this embodiment, the protein is one or more proteins selected from the group consisting of keratin 5, keratin 14, lectins, laminin 332, a6b 4-integrin, and collagen VII. In another embodiment, expression of one or more structural proteins results in an increase in fibroblasts and/or keratinocytes in the skin of the subject.
In another aspect, the invention features a method of treating or preventing squamous cell carcinoma in a subject with epidermolysis bullosa, comprising topically administering to the subject a composition comprising a therapeutically effective amount of CoQ10, thereby treating or preventing squamous cell carcinoma in the subject. In one embodiment, a composition comprising CoQ10 is administered to squamous cell carcinoma cells of a subject. In one embodiment, a composition comprising CoQ10 is administered to an area of skin in a subject comprising squamous cell carcinoma cells. In one embodiment, the squamous cell carcinoma is cutaneous squamous cell carcinoma (cSCC). In one aspect of this embodiment, the compositions of the invention are administered with an additional agent. In one aspect of this embodiment, the additional agent is a chemotherapeutic agent. In one aspect of this embodiment, the chemotherapeutic agent is cisplatin, doxorubicin, 5-fluorouracil, capecitabine, topotecan, or etoposide. In another aspect of this embodiment, the chemotherapeutic agent is 5-fluorouracil. In another aspect of this embodiment, the additional agent is diclofenac, imiquimod, or butanediol formate. In another aspect of this embodiment, the additional agent is a drug used in photodynamic therapy (PDT).
In one embodiment of the foregoing aspects, administration of a composition comprising CoQ10 modulates expression of one or more proteins in a subject, in one embodiment, the protein is a stress protein or a structural protein, in another further embodiment, the protein is selected from transaldolase 1, NM23 protein, heat shock 27kDa protein 1, keratin 14, keratin 13, proteasome β, proteasome activator subunit 3, and rho GDP dissociation inhibitor α. in another embodiment, the protein is selected from V-akt murine thymoma virus oncogene homolog 1(AKTl), oncogene 4 related to BCL2 (BAG4), BCL 2-related X protein (BAX), BCL 2-like 1(BCL2L1),
In one embodiment of any of the above aspects or embodiments, the composition comprising CoQ10 comprises 1% to 5% coenzyme Q10. In one embodiment, a composition comprising CoQ10 comprises about 1%, about 2%, about 3%, about 4%, or about 5% coenzyme Q10. In one embodiment, a composition comprising CoQ10 comprises about 3% coenzyme Q10. In one embodiment of any of the above aspects or embodiments, the composition comprising CoQ10 is administered with a second composition comprising an additional agent. In one embodiment of any of the above aspects or embodiments, the composition comprising CoQ10 further comprises an additional agent. In one embodiment of any of the above aspects or embodiments, the composition comprising CoQ10 does not comprise any other agent, i.e., coenzyme Q10 is the only active agent.
Drawings
Fig. 1 is a graph showing the effect of increasing the concentration of coenzyme Q10 exposure on cell proliferation of cancer and normal cells.
FIGS. 2A and 2B show the expression of isocitrate dehydrogenase (IDH-1) in SK-MEL-28 melanoma cells treated with increasing concentrations of coenzyme Q10 (50. mu.M or 100. mu.M). fig. 2A shows the results of Western blot of IDH-1 and β -actin loading control.fig. 2B is a graph showing the change in IDH-1 expression as a percentage of control.As shown in FIG. 2B, treatment of SK-MEL-28 cells with coenzyme Q10 is associated with a concentration-dependent increase in IDH-1 expression.
FIGS. 3A and 3B show the effect of coenzyme Q10 treatment (50 or 100. mu.M) on expression of ATP Citrate Lyase (ACL) in SK-MEL-28 melanoma cells, with Human Aortic Smooth Muscle Cells (HASMC) as a control, FIG. 3A shows Western blot results of ACL and β -actin loading controls, FIG. 3B is a graph showing the percentage change in ACL expression relative to controls, and treatment of SK-MEL-28 cells with coenzyme Q10 is associated with a concentration-dependent decrease in ACL expression, as shown in FIGS. 3A and 3B.
FIGS. 4A and 4B show the effect of coenzyme Q10 on P53, P14ARF and MDM2 expression in SK-MEL-28 melanoma cells FIG. 4A shows Western blot results of P53, P14ARF and MDM2 and β -actin loading control FIG. 4B is a graph showing the change in P53, P14ARF and MDM2 expression as a percentage of control.
FIG. 5 is a Western blot showing changes in expression of Bcl-2, Bax and Caspase 3 in SK-MEL-28 cells in response to 12 or 24 hours of coenzyme Q10 exposure (50 μ M or 100 μ M). Bcl-2, Bax and Caspase 3 are pro-and anti-apoptotic markers that modulate cell death pathways. β -actin loading controls were used.A concentration-dependent decrease in anti-apoptotic Bcl-2 protein was observed following exposure to coenzyme Q10 and a concentration-dependent increase in pro-apoptotic Bax expression was observed following exposure to coenzyme Q10.
Fig. 6 is a schematic diagram showing the structure of the Basal Membrane Zone (BMZ) of the skin.
Fig. 7 is a schematic showing the affected skin layers of an EB patient.
Fig. 8A shows a photograph of the target lesion (measured at 15.17cm) on the lower left front leg on day 1 of week 1 prior to application of CoQ10 cream. Fig. 8B shows a photograph of the same target lesion (measured at 11.2cm) on week 1, day 3. In addition to the reduction in lesion size, there is also a significant reduction in fluid within the blister. Figure 8C shows the target lesion at
Fig. 9A shows a photograph of the target lesion (measured at 5.28cm) on the medial left front thigh of a patient who developed one month prior to application of CoQ10 cream on day 1 of week 1 prior to application of CoQ10 cream. Fig. 9B shows the target lesion at
Fig. 10A shows a photograph of the target lesion (measured 36.96cm) on the medial side of the lower leg with a major blister and some granules on day 1 of week 1 prior to application of CoQ10 cream. Figure 10B shows the target lesion (measured 4cm) at
Fig. 11A shows a photograph of the target lesion (measured 21.76cm) in the upper right abdomen on day 1 of week 1 prior to application of CoQ10 cream, showing significant exudate and blood with particles near the border. Figure 11B shows a significant increase in granulation around the border for the target lesion at
Figure 12A shows photographs of target lesions (measured 20.75cm) with several granular erosions on the right lower front leg on week 1 day 1 before application of CoQ10 cream. Fig. 12B shows the target lesion at week 1, day 3 (measured 14.4cm) with a reduced number of erosions and significant re-epithelialization. Figure 12C shows the target lesion at week 8, day 1, with a further reduction in the number of erosions, and a re-epithelialized cytosis. The patients in these photographs had junctional EBs.
Figures 13A-D show a dermatoscopic image taken 48 hours post injury with the suction blister CELLUTOME system. Diffuse erythema was observed in untreated wounds (13A, 13C), while small or no areas of redness appeared in treated wounds (13B, 13D).
Fig. 14A shows the RCM image taken at week 1, and fig. 14B shows the RCM image taken at week 8. Diffuse inflammatory cell and collagen bundle disorders (fig. 14A) appeared at week 1, while inflammatory cells, organized collagen bundles and various keratinocytes were not seen at week 8 (fig. 14B).
Fig. 15A shows the RCM image taken at week 1, and fig. 15B shows the RCM image taken at week 8. At week 8 granular tissue was covered by large keratinocytes (fig. 15B), while at week 1 some inflammatory cells were seen in the granular tissue-bearing zone (fig. 15A).
Fig. 16A shows the RCM image taken at week 1, and fig. 16B shows the RCM image taken at week 8. Thicker collagen bundles appeared at week 8 (fig. 16B), and thinner collagen bundles appeared at week 1 (fig. 16A).
Detailed Description
Currently, there is no proven specific treatment for any form of EB. All types of EBs are characterized by persistent blistering of the skin and wounds requiring aggressive treatment, including the use of therapeutic methods with the ability to repair and heal wounds. EB wounds are chronic wounds, difficult to heal, and do not progress through the typical stages associated with wound healing (Pope et al, 2013). A typical wound undergoes four stages of healing: 1) hemostasis, 2) inflammation, 3) proliferation and 4) remodeling (Guo et al, 2010). In EB, the inflammatory phase is usually prolonged without proceeding to the next phase of healing. The proliferative phase may be impaired by infections, malnutrition and reduced tissue oxygenation status and reduced metabolic activity caused by drugs commonly used for symptomatic treatment, such as corticosteroids (Marinkovich et al, 2014). Remodeling of the EB can also be impaired, leading to abnormal wound contraction, resulting in severe scarring. Targeting mitochondrial function in major cell types (such as keratinocytes, fibroblasts, and endothelial cells associated with wound closure and healing) has been considered as an important mechanism for achieving improved wound healing. Mitochondrial metabolism supplies energy for wound repair, regulates keratinocyte differentiation by producing reactive oxygen species, and affects the expression of key genes in the wound healing process (Feichtinger et al, 2014).
The present invention is based on the surprising discovery that topical application of coenzyme Q10 can be used to treat patients with EB. In one embodiment, topical administration of coenzyme Q10 may be used to treat skin blisters and/or wounds in EB patients. EB patients are characterized by a deficiency and/or an insufficiency in the expression and/or activity of skin structural and functional proteins. In particular, in certain embodiments, the invention provides methods of treating a particular population of EB subjects having altered expression of one or more structural proteins in their skin, such as keratin, collagen, lectins, annexins, vimentin, fibrin, integrins, and laminin. Since the skin of subjects with EB is impaired in the expression and/or activity of these structural proteins, the healing process of wounds (e.g., skin blisters) is unique among subjects with EB. In addition to causing destructive damage to the epithelial tissue of various organs, EB patients suffer from other complications including developmental delay, anemia, muscular dystrophy and hand and foot deformities. However, the causes of morbidity and mortality in adult EBs are associated with the development of malignancies, of which cutaneous squamous cell carcinoma (sccc) is the most prominent. The present invention also reports the surprising discovery that topically applied coenzyme Q10(CoQ10) can be used to treat and/or prevent scc in EB patients.
Topical administration of a pharmaceutical composition comprising coenzyme Q10 of the invention has been shown to increase the expression of several structural proteins such as collagen, clathrin, laminin, vimentin, annexin, KRT13, KRT14 and KRT17 in fibroblasts and keratinocytes. Likewise, topical administration of CoQ10 has been shown to block the upregulation of inflammatory cytokines (IL6) and reduce the proliferation of SCC cells.
1. Definition of
Unless otherwise defined, all terms used herein have the meanings given below, and are generally consistent with the same meaning as they have to a person skilled in the art of the present invention. Practitioners specifically address Sambrook et al (1989) Molecular Cloning: A Laboratory Manual (second edition), Cold Spring harborPress, Plainview, N.Y., and Ausubel F M et al (1993) Current Protocols in Molecular biology, John Wiley & Sons, New York, N.Y., for understanding the definitions and terminology in the art. It is to be understood that this invention is not limited to the particular methodology, protocols, and medicaments described, as these may vary.
As used herein, the articles "a" and "an" refer to one or more (i.e., at least one) of the grammatical object of the article.
The term "including" is used herein to mean, and is used interchangeably with, the phrase "including but not limited to".
The term "or" is used herein to mean and is used interchangeably with the term "and/or" unless the context clearly dictates otherwise.
As used herein, the term "subject" may refer to a human or non-human animal, preferably a mammal. In a preferred embodiment, the subject is a human.
As used herein, the term "agent" or "additional agent" refers to a compound that is useful for treating epidermolysis bullosa and/or conditions or symptoms associated with EB, such as pain, inflammation, blood loss, wound healing, and the like. An "agent" or "additional agent" may further include the treatment of diseases and disorders associated with EB, such as Squamous Cell Carcinoma (SCC), anemia, and the like.
As used herein, the terms "treatment," "treating," and the like are used generally herein to refer to the amelioration of any symptoms associated with or caused by EB. As used herein, "treating" may refer to a subject experiencing one or more of the following administration of a CoQ10 composition: reduction in pain associated with EB, reduction in inflammation associated with EB, reduction in blister and/or wound formation, increased rate of healing of blisters and/or wounds, increased rate of wound closure, increased skin integrity (i.e., reduced likelihood of tearing or blistering due to mechanical or environmental stress), reduction in the number of chronic blisters and/or wounds, reduction in the amount of concomitant medications required to control symptoms of EB, and reduction in the number of blisters and/or wounds that are infected, among others. The improvement in any of these symptoms can be readily assessed according to standard methods and techniques known in the art. The population of subjects treated by the method of the disease includes subjects with any form of EB. Since EB is a genetic disease, "treatment" does not include therapeutic measures.
As used herein, the term "preventing" is used herein to refer to preventing or slowing the onset or progression of a disease or disorder, in whole or in part. As used herein, preventing refers to accomplishing one or more of the following: (a) reducing or slowing the rate of progression of the severity of the disease or disorder; (b) preventing or delaying the development of the disease or disorder as compared to the average time to onset of a subpopulation of general EB patients.
As used herein, the term "treatment duration" refers herein to the amount of time a subject applies a CoQ10 composition to a particular blister, mouth, and/or intact skin. The duration of treatment should be as long as the time required for the healing and regression of the blister and/or wound. Typically, the duration of treatment will last from about 1 week to about 12 weeks, but the treatment time may be longer for a particular blister and/or wound. Since EB is an incurable genetic disease and subjects with EB may develop new blisters and/or wounds at any time, either may be chronic if treated with standard of care. It is envisioned that a subject may have multiple blisters and/or wounds, need to administer a CoQ10 composition of the invention at any given time, and the start date of treatment and the end date of treatment may not be the same for each affected area. Furthermore, for prophylactic purposes, administration of a CoQ10 composition of the invention may not have a strict endpoint, but will be determined by the physician supervising the treatment of the subject.
As used herein, the term "therapeutically effective amount" with respect to a CoQ10 composition of the invention refers to an amount sufficient to induce a desired biological, pharmaceutical, or therapeutic result. The result can be a reduction in the signs or symptoms of a disease or disorder, or any other desired alteration of a biological system. In one embodiment of the invention, the results will relate to promoting and/or improving the healing of blisters and/or wounds, including increasing the percentage of wound closure, decreasing the healing time, improving the quality of healing, decreasing the epidermal gap distance and/or improvement of skin integrity.
As used herein, the terms "skin blistering," "skin blistering," or "blistering" refer to a particular type of wound in the upper layers of the skin (epidermis and dermis layers). In an EB, slight trauma, friction, or heat applied to the skin may form one or more skin blisters.
As used herein, the term "wound" refers to injury to living tissue, typically damage in which skin is cut or broken, caused by cutting, pounding, or other impact. Synonyms include "lesions", "tears", "comedones" and/or "splits" of the skin.
Depending on the type of EB, skin blisters and/or wounds may form due to: (1) skin splitting within the basal layer of the epidermis, (2) skin and mucosal membrane splitting at the level of the lamina lucida (the critical interface between epidermis and dermis) of the basal membrane region, and/or (3) splitting below the lamina at the level of the anchoring fibrils of the inner layer of the dermis. Any blister and/or wound may be acute or chronic.
As used herein, the term "intact skin" refers to skin in which there are no blisters and/or wounds. In certain embodiments, intact skin refers to skin that does not split within the basal layer of the epidermis, or at the level of the transparent layer of the basal membrane region, or below the stratum corneum.
As used herein, the term "structural protein" refers to a protein that maintains the epithelial integrity of the skin. In certain embodiments, the structural protein may be keratin, collagen, lectin, annexin, vimentin, fibrin, integrin, and laminin. In one embodiment, the structural protein of the invention is a protein, the defect or deficiency of which is associated with or caused by epidermolysis of bullae.
As used herein, the term "functional protein" refers to a protein having an activity essential for maintaining or reestablishing (e.g., healing) skin integrity. In certain embodiments, the functional protein may be keratin, collagen, lectin, annexin, vimentin, filaggrin, integrin, and laminin. In one embodiment, the functional protein of the invention is a protein, which is associated with or caused by a deficiency or deficiency of epidermolysis bullosa.
As used herein, the term "expression" refers to the process of producing a polypeptide from DNA. The process involves transcription of a gene into mRNA and translation of the mRNA into a polypeptide. Depending on the context of use, "expression" may refer to the production of RNA, protein, or both.
The term "level of gene expression" or "gene expression level" refers to the level of mRNA, as well as the level of precursor mRNA nascent transcripts, transcript processing intermediates, mature mRNA and degradation products, or protein encoded by the gene in the cell.
As used herein, the term "modulate" refers to either up-regulation (i.e., activation or stimulation) or down-regulation (i.e., inhibition or inhibition) of a response, or both, in combination or alone. A "modulator" is a compound or molecule that modulates, and may be, for example, an agonist, antagonist, activator, stimulator, inhibitor, or inhibitor.
As used herein, the term "altering" refers to increasing or decreasing. In certain embodiments, alteration refers to an increase or decrease in protein expression. In other embodiments, alteration refers to an increase or decrease in gene expression.
Reference will now be made in detail to the preferred embodiments of the present invention. While the invention will be described in conjunction with the preferred embodiments, it will be understood that they are not intended to limit the invention to those preferred embodiments. On the contrary, it is intended to cover alternatives, modifications, and equivalents as may be included within the spirit and scope of the invention as defined by the appended claims.
2. Pharmaceutical compositions and pharmaceutical administration
A. Coenzyme Q10
Throughout the specification, the terms "coenzyme Q" and "
wherein x is 10. CoQ10 includes a fully oxidized form (also known as ubiquinone or ubiquinolactone), a partially oxidized form (also known as semiquinone or ubiquinone), or a fully reduced form (also known as ubiquinone); or any mixture or combination thereof. In certain embodiments, the CoQ10 compound used in the methods of the invention is ubidecarenone.
CoQ10 is art-recognized and is further described in international publication numbers WO 2005/069916 (application number PCT/US2005/001581), WO2008/116135 (application number PCT/US08/57786), WO2010/132507 (application number PCT/US2010/034453), WO 2011/112900 (application number PCT/US2011/028042), and WO2012/174559 (application number PCT/US2012/043001), the entire contents of which are expressly incorporated herein by reference. CoQ10 is one of a series of
B. Composition comprising a metal oxide and a metal oxide
In a preferred embodiment, the route of administration is topical. In a related embodiment, the composition is a formulation selected from the group consisting of ointments, creams, milks, lotions and gels for topical administration. In one aspect of this embodiment, the pharmaceutical composition comprising CoQ10 is a topical cream.
The composition comprising coenzyme Q10 may be applied to a blister and/or wound site, an area of skin comprising a partial or complete blister and/or wound, intact skin or an entire skin surface, including a blister and/or wound site and intact skin, for example intact skin immediately surrounding a blister and/or wound. In certain exemplary embodiments, a composition comprising CoQ10 is applied to a blister and/or wound on the skin. In other exemplary embodiments, a composition comprising CoQ10 is applied to intact skin. In certain embodiments, the CoQ10 composition is applied after each conventional dressing change of the blister and/or wound.
The active ingredient, i.e., CoQ10, is preferably provided as a pharmaceutical formulation. For topical administration, the active ingredient may comprise from about 0.001% to about 20% w/w of the formulation in the final product, although it may comprise up to 30% w/w, preferably from about 1% to about 20% w/w of the formulation. The topical formulations of the present invention comprise the active ingredient together with one or more acceptable carriers and optionally any other therapeutic ingredients. A carrier should be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
In subjects exhibiting a disease of interest in therapy, such as subjects with epidermolysis bullosa, a therapeutically effective amount of one or more of these drugs, such as these, is administered. A therapeutically effective dose refers to an amount of a compound that results in an improvement in the symptoms or an increase in survival of the subject.
The creams according to the invention are semisolid preparations of the active ingredient for external application. They may be prepared by suspending the active ingredient in finely divided or powdered form in an aqueous or non-aqueous fluid, alone or in solution or suspension, on a substrate which may be oily or non-oily, with the aid of suitable machinery. The matrix may comprise hydrocarbons, such as hard, soft or liquid paraffin, glycerol, beeswax, metal soaps; oils of natural origin of mucus, such as almond, corn, peanut, castor or olive oil; lanolin or its derivatives, or fatty acids (such as stearic acid or oleic acid) and alcohols (such as propylene glycol or macrogels). The formulation may incorporate any suitable surfactant, for example an anionic, cationic or non-ionic surfactant, for example a sorbitan ester or a polyoxyethylene derivative thereof. Suspending agents such as natural gums, cellulose derivatives or inorganic materials such as siliceous silica, and other ingredients such as lanolin, may also be included.
In certain embodiments of the invention, methods are provided for treating epidermolysis bullosa in a subject in need thereof by topically administering to the subject an effective amount of coenzyme Q10. In one aspect of this embodiment, the topical dose of coenzyme Q10 is administered to the subject to a target skin tissue, such as a skin blister, wound, or intact skin, from about 0.01mg to about 0.5mg of coenzyme Q10 per square centimeter of skin. In one embodiment, coenzyme Q10 is applied to a target tissue, such as a skin blister, wound, or intact skin, from about 0.09 to about 0.15mg CoQ10 per square centimeter of skin. In various embodiments, coenzyme Q10 is applied to a target tissue, e.g., a skin blister, wound, or intact skin, in the range of about 0.001 to about 5.0mg, about 0.005 to about 1.0mg, about 0.005 to about 0.5mg, about 0.01 to about 0.5mg, about 0.025 to about 0.5mg, about 0.05 to about 0.4mg, about 0.05 to about 0.30mg, about 0.10 to about 0.25mg, or about 0.10 to 0.20mg of CoQ10 per square centimeter of skin. In other embodiments, coenzyme Q10 is applied to a target tissue, such as a skin blister, wound, or intact skin, at a dose of about 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.10, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.20, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.30, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.40, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.84, or 0.49mg Q per square centimeter of skin. In one embodiment, coenzyme Q10 is administered to the target tissue at a dose of about 0.12mg CoQ10 per square centimeter of skin. It is to be understood that ranges having any of these values as upper or lower limits are also intended to be part of the present invention, such as from about 0.03 to about 0.12, from about 0.05 to about 0.15, from about 0.1 to about 0.20, or from about 0.32 to about 0.49mg CoQ10 per square centimeter of skin.
In another embodiment of the invention, coenzyme Q10 is administered in the form of coenzyme Q10 cream, wherein coenzyme Q10 cream comprises between about 0.1% and 25% coenzyme Q10. In other embodiments, the CoQ10 cream comprises about 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15%, 15.5%, 16%, 16.5%, 17%, 17.5%, 18%, 18.5%, 19%, 19.5%, 20%, 20.5%, 21%, 21.5%, 22%, 22.5%, 23%, 23.5%, 24%, 24.5%, or 25% coenzyme Q10. In another embodiment of the invention, coenzyme Q10 is administered in the form of coenzyme Q10 cream, wherein coenzyme Q10 cream comprises between about 1% and 5% coenzyme Q10. In one embodiment, the coenzyme Q10 cream comprises about 3% coenzyme Q10. In other embodiments, the coenzyme Q10 cream comprises about 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5% coenzyme Q10. In various aspects of the above embodiments, about 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, or 10mg of CoQ10 cream per square centimeter of skin. It is to be understood that ranges having any of these values as upper or lower limits are also intended to be part of the present invention, such as about 0.5 to about 5.0, about 1.5 to 2.5, or about 2.5 to 5.5mg of CoQ10 cream per square centimeter of skin.
Certain aspects of the invention provide a method of treating epidermolysis bullosa in a subject in need thereof by topically administering coenzyme Q10 for treatment in a subject in need thereof, wherein the coenzyme Q10 is topically administered one or more times every 24 hours for a duration of six weeks or more. In certain aspects of the invention, the CoQ10 cream is administered twice every 24 hours for a treatment duration of about one to about twelve weeks. In certain aspects of the invention, the CoQ10 cream is administered every 24 hours for a treatment period of about one to about twelve weeks. In certain aspects of the invention, the CoQ10 cream is administered every 48 hours for a treatment duration of about one to about twelve weeks. In certain aspects of the invention, CoQ10 cream is administered twice a week for a treatment period of about one to about twelve weeks. One embodiment of the invention is a method of treating Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topically administering to the subject a pharmaceutical composition comprising a therapeutically effective amount of CoQ10, wherein treatment of the subject results in at least about a 70% reduction in the size of one or more blisters and/or wounds after about four weeks of administration of an effective amount of coenzyme Q10. One embodiment of the invention is a method of treating Epidermolysis Bullosa (EB) in a subject in need thereof, the method comprising topically administering to the subject a composition comprising a therapeutically effective amount of CoQ10, wherein treatment of the subject results in a reduction in the size of one or more blisters and/or wounds of at least about 20% to 80% after administration of a therapeutically effective amount of CoQ10 for a treatment duration of about four to eight weeks. In one aspect of the above embodiment, the reduction in size of the blister and/or wound is about 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% or 80% for any of the following treatment durations.
In any of the preceding aspects of the invention, the duration of treatment may typically be two to twelve weeks, or until blistering and/or wound healing. In one aspect of the invention, the duration of treatment is about one week. In another aspect of the invention, the duration of treatment is about two weeks. In another aspect of the invention, the duration of treatment is about three weeks. In another aspect of the invention, the duration of treatment is about four weeks. In another aspect of the invention, the duration of treatment is about five weeks. In another aspect of the invention, the duration of treatment is about six weeks. In another aspect of the invention, the duration of treatment is about seven weeks. In another aspect of the invention, the duration of treatment is about eight weeks. In another aspect of the invention, the duration of treatment is about nine weeks. In another aspect of the invention, the duration of treatment is about ten weeks. In another aspect of the invention, the duration of treatment is about eleven weeks. In another aspect of the invention, the duration of treatment is about twelve weeks. In another aspect of the invention, the duration of treatment is until the target lesion is sufficiently cured. In another aspect of the invention, the duration of treatment is chronic (no strict endpoint) because subjects with EB have habitual blisters and/or wounds that require treatment with a pharmaceutical composition comprising CoQ10 of the invention.
In a preferred embodiment of the invention, a composition comprising CoQ10 is applied to a blister on the skin. In other embodiments of the invention, a composition comprising CoQ10 is applied to intact skin. In one aspect of the invention, the pharmaceutical composition is a CoQ 103% cream described in international publication No. WO2008/116135, the entire contents of which are incorporated herein by reference in their entirety.
C. Combination therapy
In certain embodiments, CoQ10 and/or a pharmaceutical composition thereof may be treated in combination with at least one other therapeutic agent. CoQ10 and/or pharmaceutical compositions thereof and other therapeutic agents may act additively or, more preferably, synergistically. In one embodiment, coenzyme Q10 and/or a pharmaceutical composition thereof is administered concurrently with the administration of another therapeutic agent. In another embodiment, the compound and/or pharmaceutical composition thereof is administered before or after the administration of the other therapeutic agent.
In one embodiment, another agent for use in the treatment methods of the present invention is an agent that can control pain and itch and address infections with complications such as epidermolysis bullosa. Certain exemplary additional agents include vasodilators, vasoconstrictors, hypertensives, antibacterials, antibiotics, antioxidants, antifungals, non-steroidal anti-inflammatory drugs, steroids, and anesthetics.
In another embodiment, the additional agent used in the combination therapy of the present invention is a biological agent. Biologies are the products of biological systems (e.g., organisms, cells, or recombinant systems). Examples of such biological agents include nucleic acid molecules (e.g., antisense nucleic acid molecules), interferons, interleukins, colony stimulating factors, antibodies (e.g., monoclonal antibodies), anti-angiogenic agents, and cytokines. Exemplary biological agents are discussed in more detail below and generally belong to a variety of classes, including for example: hormones, hormone analogs and hormone complexes, such as estrogens and estrogen analogs, progestins, progesterone analogs and progestins, androgens, adrenocorticosteroids, antiestrogens, antiandrogens, anti-testosterone hormones, adrenal steroid inhibitors and anti-xanthophyll hormones; and enzymes, proteins, peptides, polyclonal and/or monoclonal antibodies, such as interleukins, interferons, colony stimulating factors, and the like.
It should be noted that more than one additional agent, e.g., 1, 2,3, 4, 5, may be used in combination with
Various forms of biological agents may be used. These include, but are not limited to, forms such as formalized (proform) molecules, uncharged molecules, molecular complexes, salts, ethers, esters, amides, etc., which are biologically activated upon administration to a target site.
3. Method of treatment
The present invention is based on the surprising discovery that one or more structural and/or functional proteins, such as, but not limited to, keratin, collagen, lectins, annexins, vimentin, fibrin, integrins, and laminin, are deficient in the skin of an EB subject. Thus, in certain embodiments, the present invention is directed to treating blisters and/or wounds in EB subjects by increasing the expression and/or activity of one or more structural or functional proteins that are defective in the skin of the EB subject.
A. Epidermolysis bullosa
Epidermolysis Bullosa (EB), a chronic inherited blistering skin disorder characterized by blisters and/or wounds that form when minor mechanical trauma, friction or heat is applied to the skin. The skin of subjects with EB is characterized by extreme skin fragility compared to the skin of subjects without EB. The skin of a person suffering from EB is so fragile that light abrasion and even environmental conditions (e.g., high temperature and humidity) can lead to blisters and/or wounds. The severity of EB was broad: the mildest form is localized simple EB, whose symptoms include blisters primarily on the feet and hands, but other forms, especially recessive dystrophic EB (rdeb) and borderline EB, are characterized by a broader range of skin and mucosal involvement, systemic complications, disfigurement, and often severely limits life expectancy. People with these more severe forms of EB tend to develop chronic wounds and infections for life (Pope et al, 2013).
Pearson in 1962 proposed a complex classification system for EBs based on the discovery of Transmission Electron Microscopy (TEM) (Pearson RW, 1962). Based on the level of ultrastructure formed by the splitting of the EB skin after spontaneous or minor trauma, he classifies EBs into three main types: epidermal solubility (simple EB; EBS), hemolytic (junctional EB; JEB) and skin solubility (dystrophic EB; DEB).
Advanced diagnostic techniques, such as immunofluorescent antigen mapping (IFM) or Transmission Electron Microscopy (TEM), can also be used to diagnose and classify EBs. The main advantage of TEM is that it can visualize ultrastructural abnormalities and provide a semi-quantitative assessment of specific epidermal keratinocyte-Basement Membrane Zone (BMZ) structural defects (McMillan, 1998). TEM may be particularly useful for mild DEB or EBS patients; in these cases, IFM may be normal, but TEM shows morphological abnormalities of the anchoring fibrils or intermediate filaments. Sometimes no cracks may be visible in the IFM samples, but the TEM may show ultrastructural cracks. As seen in Kindler syndrome, multiple planes of division can only be identified by TEM. The diagnostic accuracy of IFM is similar to that of TEM, which has the advantage of simpler and faster execution and interpretation. Furthermore, by using specific monoclonal antibodies, IFM can provide insight into not only the major subtype of EB, but also the structural proteins most likely to mutate (Fine et al, 2008). A recent study also showed that IFM may play a role in prenatal diagnosis of certain types of severe EB by studying chorionic villus biopsies for the first three months (D' Alessio et al, 2008).
Simple Epidermolysis Bullosa (EBS): the most common type of simple EB is localized simple EB, which undergoes skin division within the basal layer of the epidermis. blisters are usually located on the palms and soles of the feet, aggravated by heat and friction.
The primary target proteins in JEB are type XVII collagen (
Dystrophic eb (deb): this type of EB is involved in the cleavage under the dense plate in the dermis at the level of the anchoring fibrils due to mutations in the collagen VII gene. All DEB subtypes are caused by mutations in type VII collagen, the main component of anchoring fibrils. The level of cleavage occurred at the top of the blister, in the submucosa stained with collagen XVII and laminin 332. In patients with severe, systemically occult deb (rdeb), IFM showed deficient or barely detectable collagen VII. In these cases, immunostaining for type IV collagen occurred on the roof, indicating blebbing via dermal swelling, confirming DEB. Other generalized or local subtypes of DEB may show reduced or normal expression of collagen type VII (Pohla-Gubo G. et al, 2010; Cepeda-Vald é s R et al, 2010). The autosomal dominant form of inheritance is the second most common form of EB, and patients develop blisters in areas susceptible to impact or knocks, such as the toes, knees, fingers, and elbows. Autosomal recessive forms of inheritance are often more debilitating, blistering from the birth and pseudoturbinate, with toes and fingers fused together. This form is associated with chronic wounds over life and the development of invasive squamous cell carcinoma in 30 to 40 years. If they develop to mid 50 years, 90% of people will have squamous cell carcinoma.
Kindler syndrome: this rare form of EB can result in any one of the 3 levels described above being split. Early blisters are gradually replaced by scars, cuticular layers (thickening of the palms and soles), podophyllum (hyperpigmentation and hyperpigmentation, telangiectasia and atrophy) and photosensitivity. It is caused by mutations in the gene encoding kindlin1, kindlin1 involved in adhesion of basal layer keratinocytes at local contacts. Although collagen type IV and type VII antibodies may show extensive reticulocyte staining at the dermal-epidermal junction, IFM using the standard BMZ antibody panel did not show any reduction or major change in staining intensity. Labeling of normal skin with a novel polyclonal antibody against kindlin-1 showed bright staining without any skin changes in the epidermis, especially the basal keratinocytes and along the dermal-epidermal junction. In contrast, in Kindler's syndrome, there was a significant reduction in skin and in some cases no staining at all in the epidermis (ashtongh.2004).
The structure of the basal membrane region of the skin is shown in fig. 6.
EB wound healing
The inflammatory phase is the first phase of normal wound healing and occurs immediately after trauma. The first step in wound healing involves the formation of a blood clot. This is followed by inflammation, which is characterized by localized erythema, edema, and tenderness. Once this inflammation of the wound subsides, the proliferative phase of wound healing begins. Where cell proliferation, fibroblast proliferation and epidermal cell division occur. At this stage, new blood vessels are formed and granulation tissue gradually fills the wound. Once the wound is filled with granulation tissue, epidermal cell migration occurs along the wound boundary to close it. Once epidermal cell migration is complete, the final phase of wound healing, the remodeling phase, begins. At this stage, scar tissue has formed and matured, strengthened and tapered, softened and fused to the intact skin (Schober-Flores, 2003).
It is generally accepted that the wound healing process in EB patients is severely impaired, being in the inflammatory phase for a long time without entering the healing phase (Schober-Flores, 1999). The healing process is further compromised by a number of factors, including infection, nutritional status, tissue oxygenation status, and medications commonly used for symptomatic treatment, such as corticosteroids (Marinkovich et al, 2014).
As described above, patients with EB have one or more structural and/or functional proteins (such as, but not limited to, keratin, collagen, lectins, annexins, vimentin, filamin, integrins, and laminin) that alter the essential characteristics of the skin and prevent it from healing in a normal manner.
Thus, in one aspect, the invention features a method of improving the structural integrity of skin in a subject with EB, comprising topically applying to the skin a composition comprising a therapeutically effective amount of CoQ10, thereby improving the structural integrity of the skin. In one embodiment, the skin includes a defect in structural and/or functional proteins. In one embodiment, the defect in the structural and/or functional protein is within the epidermis. In another embodiment, the defect in the structural protein and/or the functional protein is within the lamina lucida. In another embodiment, the defect in the structural and/or functional protein is in the dense subplate region. In one embodiment, EB is simple epidermolysis bullosa. In another embodiment, the EB is junctional epidermolysis bullosa. In another further embodiment, the EB is dystrophic epidermolysis bullosa. In another embodiment, the defect in a structural and/or functional protein is a defect in protein activity or protein expression. In another embodiment, the lack of activity or expression is determined by histological examination, immunofluorescence imaging, or real-time quantitative PCR. In another embodiment, structural integrity is determined by histological examination, Transmission Electron Microscopy (TEM), or immunofluorescent staining from a skin biopsy of the subject.
In certain embodiments, the improvement in structural integrity is determined by quantifying the number of new blisters and/or wounds formed on the skin of a subject with EB treated with CoQ10 as compared to the skin of a subject with EB not treated with coenzyme Q10. Quantification may be determined using image analysis of photographs of blisters and/or wounds.
In another embodiment, structural integrity is measured on the skin of a subject with EB treated with CoQ10 using the suction blister test (suction blister test) as compared to the skin of a subject with EB not treated with
In another aspect, the invention features a method of treating a wound on skin of a subject, wherein the skin comprises one or more defects in structural and/or functional proteins, including topically administering a composition comprising a therapeutically effective amount of coenzyme Q10 to the wound, thereby treating the wound in the subject in another embodiment, the subject has Epidermolysis Bullosa (EB), e.g., epidermolysis bullosa is simple epidermolysis bullosa, junctional epidermolysis bullosa, dystrophic epidermolysis bullosa, or Kindler syndrome in one embodiment, the structural or functional protein is selected from keratin, collagen, lectin, annexin, vimentin, filaggrin, integrin, and laminin in another embodiment, e.g., keratin can be selected from keratin 5(KRT5), keratin 13(KRT13), keratin 14(KRT14) and keratin 17(KRT17) in another embodiment, XVII or a composition comprising collagen, collagen.
In certain embodiments, treatment of a wound is determined by the percentage wound closure relative to the initial wound area for a wound of a subject treated with CoQ10 and the percentage wound relative to the initial wound area for a wound of a subject not treated with
In another aspect, the invention features a method of increasing the rate of healing of a skin blister and/or wound in a subject with EB, comprising topically applying to the skin a composition comprising a therapeutically effective amount of coenzyme Q10(CoQ10) to the skin blister, thereby increasing the rate of healing of the skin blister and/or wound in the subject. In one embodiment, the defect in the structural and/or functional protein is within the epidermis. In another embodiment, the defect in the structural protein and/or the functional protein is within the lamina lucida. In another embodiment, the defect in the structural and/or functional protein is a dense region under the plate. In one embodiment, EB is simple epidermolysis bullosa. In another embodiment, the EB is junctional epidermolysis bullosa. In another further embodiment, the EB is dystrophic epidermolysis bullosa. In another embodiment, EB is Kindler syndrome. In another embodiment, the defect in a structural and/or functional protein is a defect in protein activity or protein expression. In another embodiment, the lack of activity or expression is determined by histological examination, immunofluorescence imaging, or real-time quantitative PCR.
In one embodiment, the rate of healing is determined from the percent wound closure of the wound of a subject treated with CoQ10 compared to the initial wound area and the wound of a subject not treated with CoQ10 relative to the initial wound area. In one embodiment, the percentage of wound closure is greater than 50%. In another embodiment, the percentage of wound closure is greater than 75%. In another embodiment, the percentage of wound closure is greater than 85%. In another embodiment, the percentage of wound closure is greater than 90%. In another embodiment, the percentage of wound closure is greater than 95%. In one embodiment, the percentage of wound closure is between 50-99%, such as 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99%. In another embodiment, the rate of healing is determined by the rate of re-epithelialization of skin blisters and/or wounds treated with CoQ10 composition compared to the rate of re-epithelialization of untreated blisters and/or wounds. In another embodiment, the rate of healing is determined by a decrease in the epidermal gap distance of a skin blister and/or wound treated with a CoQ10 composition as compared to the epidermal gap distance of an untreated skin blister and/or wound.
In one aspect, the invention features a method of treating a skin blister and/or wound in a subject in need thereof, including topically applying to the skin of the subject a composition including a therapeutically effective amount of coenzyme Q10(CoQ10), thereby treating the skin blister and/or wound of the subject. In one embodiment, the subject has Epidermolysis Bullosa (EB).
In one embodiment of the invention, administration of a composition comprising a therapeutically effective amount of CoQ10 reduces the healing time of the blister and/or wound as compared to an untreated blister and/or wound.
In another embodiment, administration of a composition comprising a therapeutically effective amount of coenzyme Q10 improves the quality of treatment with Epidermolysis Bullosa Disease Activity and Scarring Index (EBDASI) compared to untreated blisters and/or wounds.
In other aspects, the invention also features a method of promoting skin blister and/or wound healing in a subject with epidermolysis bullosa, comprising topically administering to the blister a composition comprising a therapeutically effective amount of coenzyme Q10(CoQ10), thereby promoting healing of the subject's skin blister and/or wound.
In one embodiment, the rate of re-epithelialization of blisters treated with CoQ10 compositions is increased as compared to the rate of re-epithelialization of untreated blisters. Methods for assessing re-epithelialization rates are described below.
In one embodiment, the quality of epidermal integrity of blisters and/or wounds treated with a CoQ10 composition is improved as compared to the quality of epidermal integrity of untreated blisters and/or wounds. Methods for assessing epidermal integrity are described below. In one embodiment, histological examination of the blister top is used to assess the quality of epidermal integrity.
The skin of subjects with EB lacks anchors or proteins that bind normal skin together. As a result, minimal pressure on the skin can cause blistering. The ability of CoQ10 to influence the expression of structural proteins involved in the maintenance of skin structure and function, as well as the ability to influence key stages of the wound healing process, provides convincing rationale for its utility in potential treatment of EB wounds. Thus, the present invention is based in part on the following identification: expression of certain structural proteins is increased or decreased in the skin of subjects with EB, and treatment with CoQ10 compositions of the invention can alter expression of particular structural proteins.
In certain embodiments, the expression level of one or more structural proteins in a blister and/or wound treated with a CoQ10 composition is modulated as compared to the expression level of one or more structural proteins in an untreated blister and/or wound. As a result, a particular patient population with altered expression of one or more specific structural proteins can be selected for treatment with a CoQ10 composition of the invention. Accordingly, the invention also features a method of treating a subject with epidermolysis bullosa, comprising administering to the subject a composition comprising a therapeutically effective amount of CoQ10, thereby treating the subject. In one embodiment, the subject is identified as having an alteration in the expression of one or more structural proteins in the skin.
In certain embodiments, the structural proteins include collagen, lectins, annexins, vimentin, fibrin, and laminin. In a particular embodiment, the structural protein is a keratin, in particular keratin 5(KRT5), keratin 13(KRT13), keratin 14(KRT14) and keratin 17(KRT 17).
In another aspect, the invention features a method of treating or preventing squamous cell carcinoma in a subject with a skin disorder of epidermolysis bullosa, the method including topically administering to the subject a composition including CoQ10 in a therapeutically effective amount to treat or prevent squamous cell carcinoma in the subject in one embodiment, the composition including CoQ10 is administered to squamous cell carcinoma cells of the subject in another embodiment, the squamous cell carcinoma is cutaneous squamous cell carcinoma, in one embodiment, administration of the composition including CoQ10 modulates expression of one or more proteins in the subject in another embodiment, the protein is a stress protein or a structural protein in another further embodiment, the protein is selected from the group consisting of aldolase 1, NM23 protein, heat shock 27 protein 1, keratin 14, keratin 13, proteasome activator subunit 3, and rho GDP dissociation inhibitor selected from the group consisting of murine AKT-related oncoprotein, caspase metalloprotease 466 (bnp 465), caspase metalloprotease 469, caspase metalloprotease 465, caspase, metalloprotease.
4. Method for evaluating therapeutic effect of coenzyme Q1O composition
Therapeutic effect of CoQ10 compositions in treating skin blistering and/or wounds in a subject in need thereof (e.g., a subject with EB). It can be assessed by a number of different methods, including pain reduction, improved quality of life, time to cure, quality of cure, increased resistance to drugs, and reduced blister and/or wound formation.
In certain embodiments, Epidermolysis Bullosa Disease Activity and Scarring Index (EBDASI) may be used to assess the quality of healing. EBDASI quantifies the severity of overall skin, scalp, mucous membranes, nails, and other epithelial surface involvement in motility and damage. The advantage of EBDASI is the ability to individually differentiate treatment-responsive activity scores from lesions, thereby enabling physicians to track disease activity that may be reversible to prevent lesions. EBDASI is a four-page document (see Loh et al, 2014, which is incorporated herein by reference in its entirety). In EBDASI, each area of skin is examined at 12 different anatomical sites, sequentially from top to bottom. In each zone, the activity (blistering/erosion/skinning) scored 10 points. The characteristics of the lesions (erythema, pigmentation, skin heterosis, skin atrophy, hyperkeratosis/scaling, scarring, miliaria) were 0 (absent) and 1 (present) at each site. For the scalp, mucous membranes, nails and other epithelial surfaces, the process continues.
Another framework that can be used for wound assessment is the modified measurement paradigm (MEASURE size, exudate [ quantity and characteristics ], appearance [ basal or granulation tissue ], pain [ pain ], destruction [ depth in centimeters ], re-assessment and margin) (Keast et al, 2004, incorporated herein by reference in its entirety). This document can evaluate well the wound field in the current state and is a good tool to monitor the healing process. Pope et al, 2013, outlined a modified model tailored to EB patients.
In certain embodiments, the formation of granulation tissue in EB subjects is quantified by continuous photography.
The healing time can be assessed as blistering and/or the wound being "healed" (fully closed without drainage) or "not healed". In certain embodiments, the time to healing may be defined as the first date that healing is observed minus the date that blisters and/or wounds are first found. For example, if a blister and/or wound is first identified on day 1 and is identified as "healed" on
Reflection confocal microscope
Reflection Confocal Microscopy (RCM) provides an in vivo non-invasive imaging technique, the resolution of which is closely related to histopathology at the cellular level. RCM allows rapid assessment of lesions and in vivo, and real-time visualization of epidermis and epithelium. Thus, Reflection Confocal Microscopy (RCM) can be used to assess skin blistering and/or wound healing.
In the 90's of the 20 th century, RCM was developed for real-time imaging of skin and was suitable for clinical applications. (RajadhyakshaM et al, 1999) commercially available RCM (VIVASCOPE 1500, Lucid Inc, Rochester, N.Y.) uses a near infrared 830nm diode laser and a low power laser beam of up to 22mW for imaging. The beam scans the skin to obtain a horizontal optical cross-section and presents a grey-scale image (500 x 500 μm field of view) and a mosaic image of the skin ("vivoblock"). In addition, it can produce a series of vertically stacked single images ("Vivastack") at the same point in the tissue. The maximum in vivo imaging depth of RCM is about 250-350 μm. (reflection focal microscopics: anaplas with clinical, dermocopic and histological correlations: information healthcare; 2008).
Normal skin layers (stratum corneum, spinous processes and granules, basement, dermal-epidermal junction and superficial dermis) have been characterized using RCM. These results are repeatable. In addition, other skin structures and cells have been identified, including blood vessels, hair follicles, sweat glands, keratinocytes, inflammatory cells, and melanocytes. Currently, RCM is used in basic skin studies, cosmetic studies, and clinical dermatology. Its main use is the early discovery of melanoma and non-melanoma skin. (Yamashita et al, 2005; Langley et al, 2001)
Traditionally, conventional wound assessment is based on clinical assessment of wound characteristics. Although visual inspection is an established method in clinical dermatology, this method is subjective in nature, often does not accurately describe the condition of the wound, and does not allow ultrastructural analysis of wound tissue. (Lange-Asschenfeldt et al, 2012). Thus, routine histology remains the gold standard for morphological assessment of skin wound healing. However, although histology still plays an important role in skin studies, there are still significant limitations. The invasive nature of the biopsy does not allow the same tissue region from which the biopsy is taken to be evaluated over time, and the biopsy may not be feasible for evaluating large or recurrent wounds or patients with severely impaired wound healing or a high risk of infection. In addition, tissue removal and histological processing can lead to artifacts, further limiting their clinical applicability.
Given these limitations, the applicability of many noninvasive imaging techniques to evaluate human skin wounds at different stages of wound healing has been evaluated. These techniques may be used in the present invention to assess the healing of skin blisters and/or wounds in EB patients. Among them, in vivo RCM represents an innovative optical imaging tool that allows non-invasive assessment of the skin in real time. Altintas and colleagues first evaluated burn wounds using RCM, thereby describing microcirculation, inflammation, and histomorphological aspects. Confocal microscopy has also been used to assess acute epidermal wound healing after partial laser treatment (Sattler et al, 2013) and a model of tissue damage caused by cryosurgery in humans (terthorst et al, 2011).
The fully non-invasive and high resolution capabilities of confocal microscopy make it a useful tool in wound healing studies, and in view of its cellular resolution to the epidermis and epidermal skin structures, it is particularly useful for wound healing in epidermal injury models. Characterization of the wound healing process by this study may improve our knowledge on this topic, provide a valuable tool for assessing wound healing in clinical practice, and provide a non-invasive technique to test the effects of topical products on wound healing during therapy.
In certain embodiments, the time course of wound healing is recorded by photographs, dermoscopic photographs, and RCM assessments. RCM evaluation parameters will include characteristics of skin wound repair at the cellular, morphological and structural level, as well as dynamic processes such as blood flow and inflammation and subsequent events of wound healing.
In other embodiments, Quality of Life may be measured using the Lansky Performance Scale (Lansky Performance Scale) and the child's dermatological survival Quality Index (Children's Dermatology Life Quality Index), as described herein and in Lewis-Jones et al, 1995, which is incorporated herein by reference in its entirety.
In one embodiment, the rate of re-epithelialization of blisters and/or wounds treated with a CoQ10 composition is increased as compared to the rate of re-epithelialization of untreated blisters and/or wounds. The rate of epithelialization can be assessed and compared using a Reflection Confocal Microscope (RCM).
In one embodiment, the epidermal gap distance of the blister and/or wound is reduced as compared to the epidermal gap distance of an untreated blister and/or wound. The change in epidermal gap distance can be determined by the gap width, which can be quantified as the gap distance between two migrating leading edges of the multi-layered epithelium across the blister, and expressed as the percentage of open blisters/wounds ═ width (mm) of blister/
In one embodiment, the quality of epidermal integrity of blisters and/or wounds treated with a CoQ10 composition is improved as compared to the quality of epidermal integrity of untreated blisters and/or wounds. The quality of epidermal integrity can be determined by RCM assessment.
It will be apparent to those skilled in the art that other suitable modifications and variations of the method of the invention described herein are apparent and may be made using suitable equivalents without departing from the scope of the invention or the embodiments disclosed herein. Having now described the invention in detail, it will be more clearly understood by reference to the following examples, which are included merely for purposes of illustration and are not intended to limit the invention.
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