阅读说明：本技术 双组氨酸/铁皮石斛多糖复合抗菌剂及制备方法和应用 (Double-histidine/dendrobium officinale polysaccharide composite antibacterial agent and preparation method and application thereof ) 是由 崔海英 张承慧 林琳 戴锦铭 于 2019-08-30 设计创作，主要内容包括：本发明属于食品安全领域,具体涉及一种双组氨酸/铁皮石斛多糖复合抗菌剂及制备方法和应用。铁皮石斛多糖是铁皮石斛中的主要活性成分,可有效清除自由基,且对部分细菌具有良好抗菌能力。双组氨酸是一种新型的生物自组装材料之一,合成成本较高。因此,本发明将双组氨酸和铁皮石斛多糖按一定比例制备了复合抗菌剂,且将其用于抑制磷酸缓冲液和模拟牛肉培养基中的大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus),结果显示均具有显著的抗菌效果。(The invention belongs to the field of food safety, and particularly relates to a double-histidine/dendrobium officinale polysaccharide composite antibacterial agent, and a preparation method and application thereof. The dendrobium officinale polysaccharide is a main active ingredient in dendrobium officinale, can effectively remove free radicals, and has good antibacterial capability on partial bacteria. The double-histidine is one of novel biological self-assembly materials, and the synthesis cost is high. Therefore, the composite antibacterial agent is prepared from the double histidine and the dendrobium officinale polysaccharide according to a certain proportion, and the composite antibacterial agent is used for inhibiting escherichia coli (E.coli) and staphylococcus aureus (S.aureus) in a phosphate buffer solution and a simulated beef culture medium, and the results show that the composite antibacterial agent has obvious antibacterial effect.)

1. The double-histidine/dendrobium officinale polysaccharide composite antibacterial agent is characterized by being prepared by the following method: the dendrobium officinale polysaccharide solution and the double-component amino acid solution are mixed according to a certain proportion to obtain the composite antibacterial agent, and the application of the composite antibacterial agent in the field of food safety is expanded.

2. The bi-histidine/dendrobium officinale polysaccharide composite antibacterial agent as claimed in claim 1, wherein: the dendrobium officinale polysaccharide solution is prepared by dissolving dendrobium officinale polysaccharide in sterile distilled water, so that the mass fraction of the dendrobium officinale polysaccharide is 30-40%. .

3. The bi-histidine/dendrobium officinale polysaccharide composite antibacterial agent as claimed in claim 1, wherein: the preparation method of the bi-histidine solution comprises the following steps: dissolving the double-component amino acid in ether, then placing the solution in a magnetic stirrer to stir at the rotating speed of 300rpm for 2min, and finally adding sterile distilled water to ensure that the mass fraction of the double-component amino acid solution is 20-25%.

4. The bi-histidine/dendrobium officinale polysaccharide composite antibacterial agent as claimed in claim 1, wherein: the stirring conditions were 200rpm for 1 min.

5. The double-histidine/dendrobium officinale polysaccharide composite antibacterial agent as claimed in claim 1, wherein the proportion of each component is, calculated by mass fraction, 0.5% -10.0% of double-histidine solution, 1.0% -8.0% of dendrobium officinale polysaccharide solution and the balance of sterile distilled water, and the components are mixed and then stirred uniformly by magnetic force.

6. The bi-histidine/dendrobium officinale polysaccharide composite antibacterial agent as claimed in claim 5, wherein: the magnetic stirring conditions were 200rpm for 10 min.

7. The use of the bi-histidine/dendrobium officinale polysaccharide composite antibacterial agent as defined in any one of claims 1-6, which is used for beef preservation.

Technical Field

The invention belongs to the field of food safety, and particularly relates to a double-histidine/dendrobium officinale polysaccharide composite antibacterial agent, and a preparation method and application thereof.

Background

The composite antibacterial agent can overcome the defect of the antibacterial performance of a certain single antibacterial agent and can combine the advantages of the antibacterial performance of other antibacterial agents to have stronger antibacterial function. The antibacterial agent comprises various compounding modes of organic-inorganic, organic-organic, inorganic-inorganic and the like, and finally the antibacterial agent with low cost and good antibacterial performance is obtained. Therefore, studies of complex antibacterial agents have been focused.

Polysaccharides are a class of biological macromolecules which widely exist in the nature and are one of four basic substances which form life. It not only participates in various life activities, but also has the functions of resisting tumor, virus, aging, free radical removal, bacteria and the like. Dendrobium officinale is a perennial epiphytic herb plant of the genus Dendrobium of the family Orchidaceae and is one of the famous and precious Chinese herbal medicines. It has effects of benefiting stomach, promoting fluid production, nourishing yin and clearing heat. The chemical components mainly comprise polysaccharides, alkaloids, phenanthrenes, glycosides, amino acids and the like. Researches show that the main bioactive substances in the dendrobium officinale are polysaccharide compounds and have higher content. The value of the dendrobium officinale polysaccharide in the aspects of antibacterial and antioxidant functions and product development is a hot spot of the current research.

The bishistidine (His-His, HH) is prepared by dehydration condensation of two histidines, containing two benzene rings and one end of-NH₂One end is-COOH, so it exists in the form of facultative ion at isoelectric point, and is an important element for preparing biological functional nano material, and its biological activity also has research value. At present, the material becomes a research hotspot as a novel biological self-assembly material.

According to the invention, the dendrobium officinale polysaccharide and the double-histidine are mixed in proportion to prepare the composite antibacterial agent, so that the antibacterial activity of the composite antibacterial agent is improved, the growth and the propagation of food-borne pathogenic bacteria can be effectively inhibited, and the shelf life of food is prolonged.

Disclosure of Invention

The invention aims to provide a preparation method of a double-histidine/dendrobium officinale polysaccharide composite antibacterial agent and application of the double-histidine/dendrobium officinale polysaccharide composite antibacterial agent in the field of food safety.

The preparation method of the invention is to mix the dendrobium officinale polysaccharide solution and the bi-component amino acid solution according to a certain proportion to obtain the composite antibacterial agent, and the application of the composite antibacterial agent in the field of food safety is expanded.

The dendrobium officinale polysaccharide solution is prepared by dissolving dendrobium officinale polysaccharide in sterile distilled water, so that the mass fraction of the dendrobium officinale polysaccharide is 30-40%.

The preparation method of the double-histidine solution comprises the steps of dissolving the double-histidine in ether, then placing the solution in a magnetic stirrer to stir for 2min (300rpm), and finally adding sterile distilled water to enable the mass fraction of the double-histidine solution to be 20-25%.

The composite antibacterial agent is prepared by compounding a dendrobium officinale polysaccharide solution and a double-component amino acid solution, wherein the double-component amino acid solution is 0.5-10.0% by mass, the dendrobium officinale polysaccharide solution is 1.0-8.0% by mass, and the balance is sterile distilled water, and after mixing, the mixture is magnetically stirred for 10min (200 rpm).

The composite antibacterial agent with high antibacterial activity is obtained through the synergistic effect of the two antibacterial agents, and the defects that the cost is high when the double-histidine antibacterial agent is used independently and the antibacterial activity is low when the dendrobium officinale polysaccharide is used independently are overcome.

Drawings

FIG. 1 shows the antibacterial effect of the complex antibacterial agent against Escherichia coli and Staphylococcus aureus in phosphate buffer.

FIG. 2 shows the antibacterial effect of the composite antibacterial agent on Escherichia coli and Staphylococcus aureus in beef broth.

Detailed Description

The following examples illustrate specific embodiments of the present invention, but the scope of the present invention is not limited thereto.