含硫羟基的切割试剂和氧化洗涤剂

文档序号:1745595 发布日期:2019-11-26 浏览:32次 >En<

阅读说明:本技术 含硫羟基的切割试剂和氧化洗涤剂 (The cutting reagent of sulfur-bearing hydroxyl and oxidation rinsing agent ) 是由 M·S·玛尔玛 L·安德鲁兹 B·麦克纳利 A·德鲁西亚 D·埃夫罗尼 E·卡彭特 M· 于 2017-10-28 设计创作,主要内容包括:本发明提供了使用这样的脱氧核苷三磷酸的方法、组合物、混合物和试剂盒,所述脱氧核苷三磷酸包含通过作为可切割的保护基团的包含亚甲基二硫化物的基团进行加帽的3’-O位置和可逆地联接至所述脱氧核苷的核碱基的可检测标记物。另外,描述了含硫羟基的化合物和含硫羟基的化合物的清除剂。此类化合物为未来的测序技术(包括但不限于边合成边测序(Sequencing by Synthesis))提供了新的可能性。(The present invention provides the method, composition, mixture and the kit that use such deoxynucleoside triphosphate, the deoxynucleoside triphosphate includes the detectable marker of the position 3&#39;-O being capped by the group comprising methylene based bisulfide as cleavable blocking group and the nucleobase for being reversibly attached to the deoxyribonucleoside.In addition, describing the scavenger of the compound of sulfur-bearing hydroxyl and the compound of sulfur-bearing hydroxyl.Such compound is that following sequencing technologies ((Sequencing by Synthesis) including but not limited to is sequenced in synthesis) provide a possibility that new.)

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