阅读说明：本技术 核酸修饰和鉴定方法 (Nucleic acid modification and identification method ) 是由 S.L.埃姆瑞斯 B.赖希霍尔夫 V.A.赫索格 J.朱伯 M.穆哈尔 于 2018-04-13 设计创作，主要内容包括：本发明提供了鉴定多核酸(PNA)的方法,其包括以下的步骤：提供PNA；通过添加或除去氢键键合配偶体来修饰PNA的一个或多个核碱基,从而改变一个或多个核碱基的碱基配对能力；将互补核酸与PNA进行碱基配对,包括与至少一个经修饰的核碱基进行碱基配对；至少在与至少一个经修饰的核碱基互补的位置处鉴定互补核酸的序列。(The present invention provides the methods of identification polynucleotide (PNA) comprising following step: providing PNA；One or more nucleobases of PNA are modified by adding or removing hydrogen bonding gametophyte, to change the base-pairing abilities of one or more nucleobases；Complementary nucleic acid and PNA are subjected to base pairing, including carry out base pairing at least one modified nucleobase；The sequence of complementary nucleic acid is identified at least at the position complementary at least one modified nucleobase.)