阅读说明：本技术 一种从生物碱浸膏中分离纯化红豆古碱单体的方法 (Method for separating and purifying sojanine monomer from alkaloid extract ) 是由 刘千驰 于喜洋 刘东滨 于 2021-08-31 设计创作，主要内容包括：本发明提出了一种从生物碱浸膏中分离纯化红豆古碱单体的方法,涉及化工生产技术领域。该从生物碱浸膏中分离纯化红豆古碱单体的方法包括如下步骤：将含红豆古碱的生物碱浸膏粉碎过筛,得到浸膏颗粒,将浸膏颗粒用有机溶剂萃取后得到有机层,向有机层中加入酸液酸化后再加入碱液碱化,得到生物碱溶液,将生物碱溶液超声提取后得到超声提取液,将超声提取液重结晶,即得到红豆古碱单体；此方法不仅能够有效提升分离纯化所得到的红豆古碱单体的产率,且还能够有效提高所分离纯化出的红豆古碱单体的纯度。(The invention provides a method for separating and purifying a sojanine monomer from alkaloid extract, and relates to the technical field of chemical production. The method for separating and purifying the erythronoline monomers from the alkaloid extract comprises the following steps: crushing and sieving an alkaloid extract containing the erythronoline to obtain extract particles, extracting the extract particles by using an organic solvent to obtain an organic layer, adding an acid solution into the organic layer for acidification, then adding an alkali solution for alkalization to obtain an alkaloid solution, carrying out ultrasonic extraction on the alkaloid solution to obtain an ultrasonic extraction solution, and recrystallizing the ultrasonic extraction solution to obtain an erythronoline monomer; the method not only can effectively improve the yield of the separated and purified erythronoline monomer, but also can effectively improve the purity of the separated and purified erythronoline monomer.)

1. A method for separating and purifying a sojanine monomer from an alkaloid extract is characterized by comprising the following steps of crushing and sieving the sojanine-containing alkaloid extract to obtain extract particles, extracting the extract particles by using an organic solvent to obtain an organic layer, adding an acid solution into the organic layer for acidification, then adding an alkali solution for alkalization to obtain a alkaloid solution, carrying out ultrasonic extraction on the alkaloid solution to obtain an ultrasonic extraction solution, and recrystallizing the ultrasonic extraction solution to obtain the sojanine monomer.

2. The method for separating and purifying the monomer of the erythronoline from the alkaloid extract as claimed in claim 1, wherein said acid solution is sulfuric acid, and the concentration of said sulfuric acid is 15-25%.

3. The method for separating and purifying the monomer of the erythrocupine from the alkaloid leaching paste as claimed in claim 1, wherein the alkali solution is ammonia water.

4. The method for separating and purifying the monomer of the erythronoline from the alkaloid extract of claim 1, wherein the pH of the alkaloid solution is 9 to 10.

5. The method for separating and purifying the monomer of the erythronoline from the alkaloid extract of claim 1, wherein the ultrasonic frequency of the ultrasonic extraction is 70-80Hz, and the time of the ultrasonic extraction is 70-100 min.

6. The method for separating and purifying the erythronoline monomer from the alkaloid extract as claimed in claim 1, wherein the temperature of said recrystallization is 150 ℃ and 200 ℃, and the time of said recrystallization is more than 3 h.

7. The method for separating and purifying the monomer of the erythronoline from the alkaloid extract as claimed in claim 1, wherein nanofiltration is further included after the ultrasonic extraction and before the recrystallization, and the relative molecular weight retained by the nanofiltration is 150-350.

8. The method for separating and purifying the monomers of the erythronoline from the alkaloid extract of claim 7, wherein the nanofiltration temperature is 30 to 50 ℃ and the nanofiltration pressure is 1.5 to 3.3 MPa.

9. The method for separating and purifying the monomer of the erythronoline from the alkaloid extract as claimed in claim 1, wherein the mesh number of said sieving is 40-80 mesh.

10. The method for separating and purifying the erythronoline monomers from the alkaloid extract according to any one of claims 1 to 9, wherein the preparation method of the alkaloid extract comprises the following steps of sequentially grinding, extracting with alcohol and filtering the physochlaina huashanensis to obtain a filtrate, extracting the filtrate with chloroform, and concentrating under reduced pressure to obtain the alkaloid extract.

Technical Field

The invention relates to the technical field of chemical production, and particularly relates to a method for separating and purifying a glycinin monomer from alkaloid leaching paste.

Background

Erythronoline, which is a pyrrolidine alkaloid found in physochlaina. It can also be extracted from Solanaceae plant. Erythronoline is commonly used in combination with other more potent alkaloids such as atropine or cocaine. The product has central tranquilizing effect and peripheral anticholinergic effect, and has weaker activity than atropine, but stronger effect of inhibiting gastrointestinal peristalsis and gastric juice secretion. It also has effects in dilating peripheral blood vessel, increasing coronary blood flow, and relieving asthma. Can be used for treating gastric ulcer and spasmodic pain caused by gastrointestinal diseases. Alias: 1, 3-bis (1-methyl-2-pyrrolidinyl) propanoneCAS number: 454-14-8, formula: c₁₃H₂₄N₂O, molecular weight: 224.34200, density: 0.989g/cm³，

The erythroguline has the effects of central sedation and peripheral anticholinergic action in pharmacology, has weaker activity than atropine, but has stronger action of inhibiting gastrointestinal tract peristalsis and gastric juice secretion. It also has effects in dilating peripheral blood vessel, improving coronary blood flow, and relieving asthma. However, there is no systematic and easy-to-implement method for separating and purifying erythronoline, and erythronoline is usually separated as a by-product generated in the separation process of other alkaloids such as anisodamine.

Therefore, there is a need in the market to provide a separation and purification method which can effectively improve the yield and separation purity of the erythropsis.

Disclosure of Invention

The invention aims to provide a method for separating and purifying the erythronoline from the alkaloid extract, which not only can effectively improve the yield of the separated and purified erythronoline monomer, but also can effectively improve the purity of the separated and purified erythronoline monomer.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

The embodiment of the application provides a method for separating and purifying the erythronoline from the alkaloid extract, which comprises the following steps of crushing and sieving the alkaloid extract containing the erythronoline to obtain extract particles, extracting the extract particles by using an organic solvent to obtain an organic layer, adding an acid solution into the organic layer for acidification, then adding an alkali solution for alkalization to obtain an alkaloid solution, carrying out ultrasonic extraction on the alkaloid solution to obtain an ultrasonic extracting solution, and recrystallizing the ultrasonic extracting solution to obtain an erythronoline monomer. The method comprises the steps of crushing and sieving alkaloid extract into particles with small sizes, accelerating the extraction speed of an organic solvent, improving the efficiency of the organic solvent for extracting alkaloids such as the red bean guline, acidifying by adding acid liquor to form strong acid and weak base salts, and alkalizing the strong acid and weak base salts by subsequently adding alkali liquor, thereby improving the yield of the final red bean guline monomer. The ultrasonic extraction is a new process for extracting by reasonably utilizing an ultrasonic vibration method according to a subject designed according to the existing state, polarity, solubility and the like of active ingredients and active ingredient groups in a substance, so that a solvent quickly enters a solid substance, and organic ingredients contained in the substance are dissolved in the solvent as completely as possible to obtain a multi-component mixed extracting solution. Utilize ultrasonic technology to strengthen the extraction separation process, can effectively improve the extraction separation rate, shorten extraction time, practice thrift the cost, can also improve the quality and the output of product even, and then follow-up according to the different principle of red bean ancient alkali along with temperature variation and solubility, through the mode of recrystallization in order to precipitate out red bean ancient alkali from the alkaloid solution, and then obtain the higher and higher red bean ancient alkali monomer of productivity of higher purity.

Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:

the embodiment of the invention provides a method for separating and purifying a sojanine monomer from an alkaloid extract, which preferentially crushes and screens the alkaloid extract to obtain small particles and removes large particle impurities, and effectively separates the sojanine monomer from other alkaloids by ultrasonic extraction, and in addition, the purity of the sojanine is finally improved, and the yield of the sojanine is also improved by sequentially acidifying and alkalizing acid liquor and alkali liquor.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.

The embodiment of the application provides a method for separating and purifying the erythronoline from the alkaloid extract, which comprises the following steps of crushing and sieving the alkaloid extract containing the erythronoline to obtain extract particles, extracting the extract particles by using an organic solvent to obtain an organic layer, adding an acid solution into the organic layer for acidification, then adding an alkali solution for alkalization to obtain an alkaloid solution, carrying out ultrasonic extraction on the alkaloid solution to obtain an ultrasonic extracting solution, and recrystallizing the ultrasonic extracting solution to obtain an erythronoline monomer. The method comprises the steps of crushing and sieving alkaloid extract into small-sized particles, increasing the contact area of the alkaloid extract and an organic solvent, accelerating the extraction speed of the organic solvent and improving the efficiency of the organic solvent for extracting alkaloids such as the erythropsis, wherein the erythropsis can be dissolved in an organic layer extracted by the organic solvent, taking the organic layer as a main raw material of subsequent reaction, acidifying the erythropsis and other substances generated by the erythropsis by adding acid liquor, forming strong acid and weak base salts, so that the strong acid and weak base salts can be alkalized by subsequently adding alkali liquor, and the yield of final erythropsis monomers is improved. Ultrasonic extraction, which is called ultrasonic extraction entirely, is a new process for extracting by reasonably utilizing an ultrasonic vibration method mainly according to a subject designed according to the existing state, polarity, solubility and the like of active ingredients and active ingredient groups in a substance, so that a solvent quickly enters a solid substance, and organic ingredients contained in the substance are dissolved in the solvent as completely as possible to obtain a multi-component mixed extracting solution. Utilize ultrasonic technology to strengthen extraction separation process, can effectively improve the extraction separation rate, shorten the extraction time, save cost, can also improve the quality and the output of product even, because the red bean ancient alkaloid compares other alkaloids like the solubility difference of anisodine etc. in organic solvent, extract the simple and convenient red bean ancient alkali that separates out through the ultrasonic wave according to this characteristic, and then in order to obtain the higher red bean ancient alkali monomer of purity, and then follow-up according to the different principle of red bean ancient alkali dissolubility along with temperature variation, through the mode of recrystallization in order to separate out red bean ancient alkali from the alkaloid solution, and then obtain the higher and higher red bean ancient alkali monomer of productivity of purity. Wherein the organic solvent is preferably chloroform, and the extraction effect is better than that of other organic solvents.

In some embodiments of the present invention, the acid solution is sulfuric acid, and the concentration of the sulfuric acid is 15-25%. Sulfuric acid is an inorganic compound of the formula H₂SO₄The most important oxyacid of sulfur. Pure sulfuric acid is colorless oily liquid, crystallizes at 10.36 ℃, and is usually prepared by a tower method and a contact method by using aqueous solutions of various concentrations. The former is crude dilute sulfuric acid, and the mass fraction is about 75% generally; the latter can obtain concentrated sulfuric acid with mass fraction of 98.3%, boiling point of 338 deg.C, and relative density of 1.84. The sulfuric acid has strong acidity, can react with various alkaline and neutral substances and also can react with some weakly acidic substances, so that the finally generated salt can also react with some weakly alkaline liquids, and further the yield of the finally generated erythropsis bases is improved, and the utilization rate of the alkaloid extract is improved.

In some embodiments of the present invention, the alkali solution is ammonia. The aqueous ammonia is the weak alkaline, and is convenient for volatilize, is difficult to form the sediment, consequently chooses for use the aqueous ammonia to alkalize as alkali liquor to red bean ancient alkali to improve the yield of final red bean ancient alkali, and the aqueous ammonia can volatilize at the ammonia that the in-process of reaction generated, is difficult to remain in order to generate other precipitations, and then leads to the red bean ancient alkali monomer that finally makes impure.

In some embodiments of the invention, the pH after basification is from 9 to 10. The pH interval can ensure that the amount of the generated erythropsimine monomer is not less due to too low pH, thereby resulting in lower yield of the finally generated erythropsimine monomer. And the excessive generation of precipitate and other alkaline substances caused by the excessive pH value can not occur, so that the purity of the finally generated jalapine monomer is low.

In some embodiments of the present invention, the ultrasonic frequency of the ultrasonic extraction is 70-80Hz, and the ultrasonic extraction time is 70-100 min. Ultrasonic extraction, which is called ultrasonic extraction entirely, is a subject designed mainly according to the existing state, polarity, solubility and the like of active ingredients and active ingredient groups in substances, and is a new process for extracting by reasonably utilizing an ultrasonic vibration method, so that a solvent quickly enters solid substances, and organic ingredients contained in the substances are dissolved in the solvent as completely as possible to obtain a multi-component mixed extracting solution. The ultrasonic technology is utilized to strengthen the extraction and separation process, the extraction and separation rate can be effectively improved, the extraction time is shortened, the cost is saved, and the product quality and yield can be even improved; and this time interval can guarantee that the red bean ancient alkali draws from the alkaloid solution completely, and then improves the productivity of the red bean ancient alkali who makes, is less than this time interval when the extraction time and can lead to the red bean ancient alkali can't draw from the alkaloid solution completely and draw out, and then leads to the productivity of red bean ancient alkali to reduce, and then can lead to more other alkaloid impurity to follow to separate out when the extraction time is longer than this time interval, and then leads to the purity of the red bean ancient alkali who makes to reduce.

In some embodiments of the present invention, the temperature of the recrystallization is 150-. Recrystallization can make the sojanine monomer separate out from it, this temperature interval can guarantee to make as many as possible of the sojanine monomer separate out from the extract after the supersound is drawed, can also avoid other alkaloids to separate out along with the sojanine monomer jointly, and then guaranteed the monomeric purity of the sojanine that finally obtains, and this time interval can guarantee to make the sojanine monomer separate out from the extract completely, when being less than this time interval, the sojanine monomer probably separates out incompletely, and then lead to the monomeric productivity of sojanine to reduce.

In some embodiments of the present invention, the ultrasonic extraction and the recrystallization further comprise nanofiltration, wherein the nanofiltration retains a relative molecular weight of 150-. Nanofiltration is a pressure-driven membrane separation process between reverse osmosis and ultrafiltration, and the pore diameter range of the nanofiltration membrane is about a few nanometers. Occurs later than in other pressure-driven membrane separation processes. Its appearance dates back to the research of NS-300 membranes of j.e.cadotte at the end of the 70 s, after which nanofiltration developed rapidly and membrane modules were commercialized in the middle of the 80 s. Nanofiltration membranes are mostly derived from reverse osmosis membranes, such as CA, CTA membranes, aramid composite membranes, sulfonated polyethersulfone membranes, and the like. Nanofiltration (NF) is used to separate small molecular weight substances, such as inorganic salts or small organic molecules like glucose, sucrose, etc., from the solvent. Nanofiltration, also known as low pressure reverse osmosis, is an emerging field of membrane separation technology, and has separation performance between reverse osmosis and ultrafiltration, allowing some inorganic salts and some solvents to permeate through the membrane, thereby achieving separation effect. In the alkaloid solution, a small amount of anisodine and other alkaloid impurities are also contained, wherein the molecular weight of anisodine is 319, and the molecular weight of erythrosine is 224, so that the alkaloid with the intercepted relative molecular weight in the interval can ensure that more erythrosine monomers are separated out as much as possible, and simultaneously, the other alkaloids are separated out as little as possible, thereby improving the purity and yield of the prepared erythrosine monomers.

In some embodiments of the invention, the nanofiltration temperature is 30-50 ℃ and the nanofiltration pressure is 1.5-3.3 MPa. The temperature interval and the pressure interval can ensure that the erythropsimine is separated out as much as possible and reduce the separation of other impurities. When the temperature of nanofiltration is too low or too high, other alkaloids can be filtered out along with the jalapine, and then the purity that leads to the jalapine is lower, and the pressure of nanofiltration is too low or too high then can lead to the jalapine can't filter out completely, and then leads to the reduction of the yield of jalapine.

In some embodiments of the invention, the mesh size of the screen is 40-80 mesh. The mesh interval can ensure that the sizes of alkaloid extract particles are proper, so that the situation that the sizes of the extract particles which are sieved out are too small due to too large sieving mesh number and some large-particle impurities containing the erythropsis chinensis are sieved out, and the yield of the finally prepared erythropsis chinensis monomer is reduced is avoided. And the screening mesh number is too small, so that more impurities are screened out together with the red bean ancient alkali, and the purity of the red bean ancient alkali monomer is reduced.

In some embodiments of the present invention, the preparation method of the alkaloid extract comprises the following steps of sequentially grinding, extracting with ethanol and filtering the physochlaina huashanensis to obtain a filtrate, extracting the filtrate with chloroform, and concentrating under reduced pressure to obtain the alkaloid extract. The method discloses a preparation method of an alkaloid extract, wherein part of impurities which are difficult to remove can be primarily removed by extracting with chloroform. Radix Physochlainae is dry root of Physochlaina Pubescens of Solanaceae. Plucked in spring, cleaned, and dried in the sun. Distributed from the middle of Qin mountain in Shaanxi to the east, the west and south of Henan, and the south of Shanxi. Has effects of warming lung, eliminating phlegm, relieving asthma and cough, tranquilizing mind, and relieving convulsion. Can be used for treating cough and asthma due to cold phlegm, palpitation, and insomnia. Therefore, in order to ensure the yield of the erythronoline monomer, the physochlaina is selected as the main raw material for preparing the extract, and other plants containing the erythronoline such as coca and the like can also be selected.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

The embodiment provides a method for separating and purifying the erythronoline from the alkaloid extract, which comprises the following steps of sequentially grinding, extracting with alcohol and filtering the physochlaina huashanensis to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure to obtain an alkaloid extract, crushing and sieving with a 40-mesh sieve to obtain extract particles, extracting the extract particles with chloroform, taking an organic layer, acidifying with 15% sulfuric acid, adding ammonia water to obtain a alkaloid solution with the pH of 9, extracting the alkaloid solution with 70Hz ultrasonic waves for 70min to obtain an ultrasonic extracting solution, carrying out nanofiltration on the ultrasonic extracting solution at the temperature of 30 ℃ under the pressure of 1.5MPa and according to the intercepted relative molecular weight of 150, and recrystallizing at the temperature of 150 ℃ for 3.1h to obtain the erythronoline monomer.

Example 2

The embodiment provides a method for separating and purifying the erythronoline from the alkaloid extract, which comprises the following steps of sequentially grinding, extracting with alcohol and filtering the physochlaina huashanensis to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure to obtain the alkaloid extract, crushing and sieving with a 80-mesh sieve to obtain extract particles, extracting the extract particles with chloroform, taking an organic layer, acidifying with 25% sulfuric acid, adding ammonia water to obtain a alkaloid solution with the pH of 10, extracting the alkaloid solution with 80Hz ultrasonic waves for 100min to obtain an ultrasonic extract, carrying out nanofiltration on the ultrasonic extract at 50 ℃ under the pressure of 3.3MPa and the intercepted relative molecular weight of 350, and recrystallizing at 200 ℃ for 3.2h to obtain the erythronoline monomer.

Example 3

The embodiment provides a method for separating and purifying sojanine from alkaloid extract, which comprises the following steps of sequentially grinding, extracting with alcohol and filtering physochlaina huashanensis to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure to obtain alkaloid extract, crushing and sieving with a 60-mesh sieve to obtain extract particles, extracting the extract particles with chloroform, taking an organic layer, acidifying with 20% sulfuric acid, adding ammonia water to obtain a alkaloid solution with the pH of 9.5, extracting the alkaloid solution with 75Hz ultrasonic waves for 85min to obtain an ultrasonic extracting solution, performing nanofiltration on the ultrasonic extracting solution at 40 ℃ under the pressure of 2.4MPa and the interception relative molecular weight of 250, and recrystallizing at 175 ℃ for 3.5h to obtain a sojanine monomer.

Example 4

The embodiment provides a method for separating and purifying sojanine from alkaloid extract, which comprises the following steps of sequentially grinding, extracting with alcohol and filtering physochlaina huashanensis to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure to obtain alkaloid extract, crushing and sieving with a 50-mesh sieve to obtain extract particles, extracting the extract particles with chloroform, taking an organic layer, acidifying with 18% sulfuric acid, adding ammonia water to obtain a alkaloid solution with the pH of 9.3, extracting the alkaloid solution with 72Hz ultrasonic waves for 80min to obtain an ultrasonic extracting solution, performing nanofiltration on the ultrasonic extracting solution at 36 ℃ under the pressure of 2.0MPa and the interception relative molecular weight of 210, and recrystallizing at 170 ℃ for 4.5h to obtain a sojanine monomer.

Example 5

The embodiment provides a method for separating and purifying the erythronoline from the alkaloid extract, which comprises the following steps of sequentially grinding, extracting with alcohol and filtering the physochlaina huashanensis to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure to obtain an alkaloid extract, crushing and sieving with a 70-mesh sieve to obtain extract particles, extracting the extract particles with chloroform, taking an organic layer, acidifying with 24% sulfuric acid, adding ammonia water to obtain an alkaloid solution with the pH of 9.7, extracting the alkaloid solution for 90min by adopting 78Hz ultrasonic waves to obtain an ultrasonic extracting solution, carrying out nanofiltration on the ultrasonic extracting solution at the temperature under the pressure of 2.8MPa and the intercepted relative molecular weight of 280, and recrystallizing at the temperature of 180 ℃ for 5h to obtain the erythronoline monomer.

Example 6

The embodiment provides a method for separating and purifying sojanine from alkaloid extract, which comprises the following steps of sequentially grinding, extracting with alcohol and filtering physochlaina huashanensis to obtain filtrate, extracting the filtrate with chloroform, concentrating under reduced pressure to obtain alkaloid extract, crushing and sieving with a 60-mesh sieve to obtain extract particles, extracting the extract particles with chloroform, taking an organic layer, acidifying with 16% sulfuric acid, adding ammonia water to obtain a alkaloid solution with the pH of 9.1, extracting the alkaloid solution with 71Hz ultrasonic waves for 81min to obtain an ultrasonic extracting solution, performing nanofiltration on the ultrasonic extracting solution at 39 ℃ under the pressure of 2.9MPa according to the interception relative molecular weight of 260, and recrystallizing at 155 ℃ for 4h to obtain a sojanine monomer.

Test example 1

The monomer of the red bean guline prepared in the examples 1-6 is taken and weighed (g), then the same 50g of radix physochlainae is taken and the alkaloid extract and the monomer of the red bean guline are prepared by adopting the traditional separation and purification process on the market and weighed (g), the group is taken as a comparative example, and then according to the formula: the monomer yield (%) of the erythrocupine was calculated as the weight of the monomer/the weight of the alkaloid extract × 100%, and the results are shown in table 1.

TABLE 1 monomer yield table of red bean ancient alkali

As can be seen from the results in Table 1, the yield of the monomer of erythronoline obtained in examples 1 to 6 of the present invention was slightly higher than that of the monomer of erythronoline obtained in the prior art, and thus it can be seen that the process for producing the monomer of erythronoline provided by the present invention can improve the yield of the monomer of erythronoline.

Test example 2

The components and the content of the erythropsimine in the monomer of the erythropsimine prepared in the examples 1 to 6 are detected by chromatography in sequence, wherein the monomer of jaspertine prepared in example 1 contained another higher peak in addition to the jaspertine peak, wherein the monomer of glycocoll prepared in example 2 contained a lower peak in addition to the peak of glycocoll, wherein the monomer of glycocoll prepared in example 3 contains two peaks higher than the peak of glycocoll, wherein the monomer of caroguline prepared in example 4 contained two lower peaks in addition to the caroguline peak, wherein the monomer of glycocoll prepared in example 5 contained a higher peak and a lower peak in addition to the peak of glycocoll, wherein the monomer of glycocoll prepared in example 6 contained a higher peak in addition to the peak of glycocoll.

And (3) taking the jalapine monomer prepared in the comparative example, and detecting the components and the content of the jalapine monomer by adopting a chromatography method, wherein the jalapine monomer prepared in the comparative example contains three lower peak values and two higher peak values besides the jalapine peak value.

According to the results, the peak value of the chromatogram of the jalapine monomer obtained in the embodiment of the invention is less than that of the chromatogram of the jalapine monomer obtained in the comparative example, so that the impurities contained in the jalapine monomer obtained in the embodiment of the invention are obviously lower than those contained in the jalapine monomer prepared in the comparative example, and therefore, the conclusion can be drawn that the purity of the jalapine monomer obtained by the production process of the jalapine monomer provided by the invention is obviously higher than that of the current production process on the market.

In conclusion, the method preferentially crushes and screens the alkaloid extract into small particles and removes large particle impurities, and effectively separates the monomers of the erythropsimine from other alkaloids by ultrasonic extraction, and in addition, acidification and alkalization are sequentially carried out by acid liquor and alkali liquor, so that the purity of the erythropsimine is finally improved, and the yield of the erythropsimine is also improved.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.