阅读说明：本技术 一种大豆蛋白质的提取方法 (Method for extracting soybean protein ) 是由 黄乃泰 于 2020-06-16 设计创作，主要内容包括：本发明属于大豆蛋白提取领域,尤其是一种大豆蛋白质的提取方法,针对现有的大豆蛋白提取效率较低,且提取效果不好的问题,其包括以下步骤：选取成熟的黄豆筛去砂石后烘干去皮；将黄豆去油粉碎后研磨成黄豆粉；将黄豆粉与氢氧化钠溶液进行混合,经过两次碱性浸取之后分离得到固料和两组浸取液；将两组浸取液进行混合；将混合的溶液与盐酸溶液进行混合,使混合后的溶液PH达到4-5,然后沉淀得到沉淀液；将沉淀液离心得到蛋白粗品固料；将蛋白粗品加水,使PH值达到7-8,然后灭菌干燥得到蛋白粉。本发明对传统工艺做出改进,能够大大提高大豆蛋白的提取效率,并能提高大豆蛋白的提取质量。(The invention belongs to the field of soybean protein extraction, and particularly relates to a method for extracting soybean protein, which aims at solving the problems of low extraction efficiency and poor extraction effect of the existing soybean protein and comprises the following steps: selecting ripe soybeans, screening sand, drying and peeling; deoiling and crushing soybeans, and grinding the soybeans into soybean powder; mixing soybean powder with a sodium hydroxide solution, and separating after two times of alkaline leaching to obtain a solid material and two groups of leaching solutions; mixing the two groups of leaching solutions; mixing the mixed solution with a hydrochloric acid solution to enable the pH of the mixed solution to reach 4-5, and then precipitating to obtain a precipitation solution; centrifuging the precipitation solution to obtain a protein crude product solid material; adding water into the crude protein product to make the pH value reach 7-8, and sterilizing and drying to obtain the protein powder. The invention improves the traditional process, can greatly improve the extraction efficiency of the soybean protein and can improve the extraction quality of the soybean protein.)

1. A method for extracting soybean protein is characterized by comprising the following steps:

(1) selecting materials: selecting ripe soybeans, screening the selected soybeans by using a vibrating screen to remove gravels in the soybeans, and then drying and peeling the screened soybeans;

(2) grinding: removing oil from soybeans, then crushing soybean meal, and screening the crushed soybean meal to obtain soybean meal;

(3) alkaline leaching: preparing a sodium hydroxide solution;

the first leaching, namely mixing the soybean meal with a sodium hydroxide solution, stirring the mixture of the soybean meal and the sodium hydroxide, performing solid-liquid separation on the mixture of the soybean meal and the sodium hydroxide after stirring for two to three hours, and separating to obtain a first leaching solution and a first solid material;

leaching for the second time, namely mixing the first solid material with a sodium hydroxide solution, stirring the mixture of the first solid material and the sodium hydroxide, performing solid-liquid separation on the mixture of the first solid material and the sodium hydroxide after stirring for two to three hours, and separating to obtain a second leaching solution and a second solid material;

mixing: mixing the separated first leaching solution with the second leaching solution;

acid precipitation: preparing a hydrochloric acid solution, pouring the hydrochloric acid solution into a mixed solution of a first leaching solution and a second leaching solution, stirring the hydrochloric acid solution and the mixed solution, detecting the pH value of the neutralized hydrochloric acid solution and the mixed solution to enable the pH value to reach 4-5, and then precipitating the neutralized hydrochloric acid solution and the mixed solution;

centrifuging; centrifuging the precipitated solution by using a centrifuge, wherein a third solid material obtained by centrifuging is a crude product of the isolated soy protein;

drying, adding water into the crude soybean protein product to adjust the pH value to 7-8, sterilizing and drying to obtain the isolated soybean protein powder.

2. The method of claim 1, wherein in step 3, the sodium hydroxide solution in the first leaching is 12 times the weight of the soybean meal, and the sodium hydroxide solution in the second leaching is 6 times the weight of the first solid material.

3. The method of claim 1, wherein in step 2, the soybeans are deoiled by an oil press, and the particle size of the deoiled and pulverized soybeans is less than 2 mm.

4. The method for extracting soybean protein according to claim 1, wherein in step 7, the sterilization is performed by pasteurization.

5. The method for extracting soybean protein according to claim 1, wherein in step 7, the crude soybean is dried and sterilized in a drying oven while being sterilized by ultraviolet light.

6. The method for extracting soybean protein according to claim 1, wherein in step 4, the first extract and the second extract are mixed by a mixing barrel, the mixing shaft is driven by a motor in the mixing barrel to stir and mix the first extract and the second extract, and an electric heating tube is arranged on the outer wall of the mixing barrel to heat so that the mixing temperature is controlled at fifty degrees.

7. The method for extracting soybean protein according to claim 1, wherein in step 6, the solution after the precipitation is centrifuged by a centrifuge, and the centrifuged solution is filtered again by a filter membrane, wherein the filter membrane is a microfiltration membrane.

Technical Field

The invention relates to the technical field of soybean protein extraction, in particular to a method for extracting soybean protein.

Background

The soybean protein has good functional characteristics and high protein nutrition, and is widely applied to the food industry. The yield of the soybean protein is effectively improved in the production process, and the method is very important for reducing the cost and improving the benefit. At present, in the field of deep processing of soybeans, the main extraction method of soybean protein is an alkali-soluble acid-precipitation method.

The existing method for extracting the soybean protein by using the alkali-soluble acid-precipitation method has certain defects in the production process, the extraction efficiency of the soybean protein is low, and the quality of the extracted soybean protein is not high, so that the method for extracting the soybean protein is provided at present.

Disclosure of Invention

The extraction method of the soybean protein provided by the invention solves the problems of low extraction efficiency and poor extraction effect of the existing soybean protein.

In order to achieve the purpose, the invention adopts the following technical scheme:

a method for extracting soybean protein comprises the following steps:

(1) selecting materials: selecting ripe soybeans, screening the selected soybeans by using a vibrating screen to remove gravels in the soybeans, and then drying and peeling the screened soybeans;

(2) grinding: removing oil from soybeans, then crushing soybean meal, and screening the crushed soybean meal to obtain soybean meal;

(3) alkaline leaching: preparing a sodium hydroxide solution;

the first leaching, namely mixing the soybean meal with a sodium hydroxide solution, stirring the mixture of the soybean meal and the sodium hydroxide, performing solid-liquid separation on the mixture of the soybean meal and the sodium hydroxide after stirring for two to three hours, and separating to obtain a first leaching solution and a first solid material;

leaching for the second time, namely mixing the first solid material with a sodium hydroxide solution, stirring the mixture of the first solid material and the sodium hydroxide, performing solid-liquid separation on the mixture of the first solid material and the sodium hydroxide after stirring for two to three hours, and separating to obtain a second leaching solution and a second solid material;

(4) mixing: mixing the separated first leaching solution with the second leaching solution;

(5) acid precipitation: preparing a hydrochloric acid solution, pouring the hydrochloric acid solution into a mixed solution of a first leaching solution and a second leaching solution, stirring the hydrochloric acid solution and the mixed solution, detecting the pH value of the neutralized hydrochloric acid solution and the mixed solution to enable the pH value to reach 4-5, and then precipitating the neutralized hydrochloric acid solution and the mixed solution;

(6) centrifuging; centrifuging the precipitated solution by using a centrifuge, wherein a third solid material obtained by centrifuging is a crude product of the isolated soy protein;

(7) drying, adding water into the crude soybean protein product to adjust the pH value to 7-8, sterilizing and drying to obtain the isolated soybean protein powder.

Preferably, in the step 3, the weight of the sodium hydroxide solution in the first leaching process is 12 times of that of the soybean meal, and the weight of the sodium hydroxide solution in the second leaching process is 6 times of that of the first solid material.

Preferably, in the step 2, the soybeans are deoiled through an oil press, and the particle size of the deoiled and pulverized soybeans is less than 2 mm.

Preferably, in step 7, the sterilization is performed by pasteurization.

Preferably, in step 7, the crude soybean is put into a drying oven for drying and sterilization, and is sterilized by an ultraviolet lamp.

Preferably, in the step 4, the first leaching solution and the second leaching solution are mixed through a mixing barrel, the mixing barrel is internally provided with a motor to drive a mixing shaft to mix the first leaching solution and the second leaching solution, and an electric heating tube on the outer wall of the mixing barrel is used for heating, so that the mixing temperature is controlled at fifty degrees.

Preferably, in the step 6, the solution after the precipitation is centrifuged by the centrifuge, and then the centrifuged solution is filtered again by the filter membrane, wherein the filter membrane is a microfiltration membrane.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the invention, when the first leaching solution and the second leaching solution obtained after the first leaching and the second leaching are separated are mixed, the first leaching solution and the second leaching solution are mixed by using the mixing barrel, the stirring blades in the mixing barrel are driven by the motor to stir, so that the first leaching solution and the second leaching solution can be quickly and fully mixed, the mixing speed of the first leaching solution and the second leaching solution is accelerated, the mixing barrel is controlled at fifty ℃ by using the electric heating tube, the mixing speed of the first leaching solution and the second leaching solution can be accelerated at the temperature, the mixing time of the first leaching solution and the second leaching solution is saved, the mixing efficiency can be greatly improved, the subsequent precipitation centrifugal drying is more effective, and the time required by the whole extraction process is saved.

2. According to the invention, after the precipitation liquid is centrifuged, the centrifuged solution is filtered again through the filtering membrane, when the centrifuge is used for centrifuging the precipitation liquid, some solid materials can be remained in the centrifuged liquid, and the residual solid materials can be sufficiently filtered by utilizing the filtration of the microporous filtering membrane, so that the solid materials in the precipitation liquid can be sufficiently filtered, the waste of the solid materials in the precipitation liquid is avoided, and the extraction quality of the soybean protein is improved.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.