阅读说明：本技术 用于从生物样品、优选加工过的植物籽粕分离膜结合的蛋白质的方法和手段 (Methods and means for separating membrane-bound proteins from biological samples, preferably processed plant seed meal ) 是由 M·K·威瑞克 M·夏 J·斯莱萨列娃 于 2018-12-12 设计创作，主要内容包括：本发明提供了从生物样品中,例如在来自粗的或加工过的植物材料、优选脱脂的植物籽粕例如卡诺拉油菜籽粕的样品中有效分离膜结合蛋白的方法和手段。所述生物样品可以被高度加工,例如通过施加高温、压力或化学处理,并且可以来自种子基质以及其他典型的植物组织,例如种子、谷粒、叶片、根或花粉。本发明包括提供新的提取缓冲液(MEB)以及其在本发明方法中的应用,其中所述缓冲液具有10-12.5的强碱性pH并且包含0.5％-5％水平的可溶浓度的去污剂。(The present invention provides methods and means for efficiently separating membrane-associated proteins from biological samples, for example, samples derived from crude or processed plant material, preferably defatted plant seed meal such as canola meal. The biological sample may be highly processed, for example by application of heat, pressure or chemical treatment, and may be from seed substrates as well as other typical plant tissues, such as seeds, grains, leaves, roots or pollen. The present invention comprises providing a novel extraction buffer (MEB) and its use in the method of the invention, wherein the buffer has a strongly alkaline pH of 10-12.5 and comprises a soluble concentration of detergent at a level of 0.5-5%.)