阅读说明：本技术 一种水溶性硒蛋白生产制备方法 (Method for preparing water-soluble selenoprotein ) 是由 柯昌英 于 2020-04-20 设计创作，主要内容包括：本发明公开了一种水溶性硒蛋白生产制备方法,通过针对地方优质特色资源,以富硒大豆为原料,经进一步的富硒化培养技术得富含有机硒400ppm、全蛋白质含量20％以上的富硒芸豆,以此富硒芸豆为原料,综合运用现代生物技术,采用一系列的生化方法,使储存于大豆中的有机硒蛋白游离出来,后经分离、纯化、干燥等工艺过程制取水溶性硒蛋白,为食品、药品、保健品及种植业、养殖业等领域提供无毒高效的生物活性硒原料,本发明涉及食品生产技术领域。该水溶性硒蛋白生产制备方法,提取过程中无化学污染,绿色安全,被浸提的生物活性物质在短时间内保持不变,通过打浆搅拌作用等可加速植物有效成分的溶出,从而提高了提取率。(The invention discloses a method for preparing water-soluble selenium protein, which uses selenium-rich soybean as a raw material and obtains selenium-rich kidney bean with the content of total protein of more than 20 percent and the content of organic selenium of 400ppm by further selenium-rich culture technology aiming at local high-quality characteristic resources. The preparation method of water soluble selenoprotein has no chemical pollution in extraction process, is green and safe, the extracted bioactive substances are kept unchanged in a short time, and the dissolution of plant effective components can be accelerated by pulping and stirring, so that the extraction rate is improved.)

1. A method for preparing water-soluble selenoprotein is characterized by comprising the following steps: the method specifically comprises the following steps:

s1, seed collection: collecting selenium-rich bean seeds from an Enshi selenium-rich area;

s2, preprocessing: cleaning and drying the bean seeds collected in the step S1 by using cleaning equipment, removing impurities by using a 40-80-mesh vibrating screen, and crushing the dried bean raw material at a low temperature of below 60 ℃ into chips with the particle size of 80-200 meshes to obtain kidney bean powder;

s3, extraction processing, which comprises the following steps:

1, grinding the kidney bean powder obtained in the step S2 into slurry, extracting for 3-5h at the temperature of 40-50 ℃ according to the material-liquid ratio of 1:25-30, and then carrying out separation treatment to obtain precipitate and filtrate which mainly contain starch and fiber;

a2, extracting the precipitate obtained in the step a1 for the second time to obtain protein, wherein the ratio of material to liquid is 1:15-20, extracting for 2-5h at the temperature of 40-50 ℃, and then separating to obtain filtrate;

a3, combining the two filtrates obtained in the separation treatment in the steps a1 and a2, and carrying out separation treatment again to separate out soluble protein and micromolecular sugar;

a4, and mixing the soluble protein obtained in the step a3 with a mixture of 1:20, diluting with purified water, and decolorizing with active carbon column;

a5, adsorbing sugar molecules and nucleic acid molecules again by the decolorized solution through an adsorption column, and further purifying the selenoprotein;

a6, dialyzing and concentrating the decolored solution by using a molecular membrane to remove free micromolecular substances;

a7, concentrating the dialyzed concentrated solution under vacuum and reduced pressure to 30 Baume degree, and freeze-drying or spray-drying at low temperature.

2. The method for preparing water-soluble selenoprotein of claim 1, wherein the method comprises the following steps: the seed collected in step S1 is characterized by: uniform size, and is full and healthy.

3. The method for preparing water-soluble selenoprotein of claim 1, wherein the method comprises the following steps: the separation treatment in the steps a1 and a2 is carried out by adopting a tubular centrifuge with the separation factor of 15000G.

4. The method for preparing water-soluble selenoprotein of claim 1, wherein the method comprises the following steps: the separation treatment in the step a3 is carried out by adopting a tubular centrifuge with a separation factor of 19000G.

5. The method for preparing water-soluble selenoprotein of claim 1, wherein the method comprises the following steps: the step a5 is to use a No. 50 macroporous resin adsorption column for treatment.

6. The method for preparing water-soluble selenoprotein of claim 1, wherein the method comprises the following steps: the pore size of the molecular membrane in the step a6 is 0.001 micron.

Technical Field

The invention relates to the technical field of food production, in particular to a method for producing and preparing water-soluble selenoprotein.

Background

Selenium is an essential trace element for human bodies, and selenium deficiency can cause various diseases, so that the selenium plays an extremely important role in human health. The selenium element of human body mainly comes from food, the selenium in the food is influenced by the environment such as geographical soil, three quarters of countries and regions in the world are deficient in selenium, China is a selenium deficient country, 2/3 regions belong to selenium deficiency, even 29% of the regions are serious selenium deficiency, the environment is worsened at present, and the selenium element becomes an important factor influencing the human health.

In 1976, the food selenium content in the disease areas of keshan disease and Kaschin-Beck disease is generally low, and the patients who supplement selenium have extremely obvious treatment effect. Adult dietary selenium intake is set by countries all over the world, and in 2015, the US FDA formally lists selenium as an essential nutrient element.

The kidney bean is commonly named as kidney bean, sword bean and the like, is rich in nutrition, wherein the vitamins, mineral elements and protein are relatively comprehensive, the content of kidney bean protein reaches 23.58 percent, the protein contains more than 18 amino acids, and the potassium content and the magnesium content are high while the sodium content is low. The selenium-rich kidney bean is the kidney bean which has the total selenium of more than 20 mug/100 g and the organic selenium content of more than or equal to 80 percent and meets the standard of DBS 42/002-2014.

The water-soluble selenoprotein powder adopts all the raw materials which are high in safety and practicability and are green and environment-friendly food materials, can provide abundant nutrition while scientifically supplementing selenium for consumers, and can play a role in strengthening metabolism and enhancing cell activity after being eaten for a long time. Chinese traditional medicine research institute believes that selenium-rich kidney beans can be used as anti-aging Chinese herbal medicines.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a preparation method of water-soluble selenoprotein, which is green, simple, easy to implement and high-efficiency, has beautiful product color, can keep part of nutritional ingredients in kidney beans stable for a long time, and simultaneously has the advantages of beautiful appearance, light yellow color, good water solubility, high selenium content and the like, and has good edibility, high protein content, cancer prevention, immunity improvement and other effects.

(II) technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme: a method for producing and preparing water-soluble selenoprotein, it is starting with the seed of selenium-enriched beans, through the pure physical extraction way of the low temperature, protect the selenoprotein from the environmental change of the temperature, protect the activity of selenoprotein, include the following steps specifically:

s1, seed collection: collecting selenium-rich bean seeds from an Enshi selenium-rich area;

s2, preprocessing: cleaning and drying the bean seeds collected in the step S1 by using cleaning equipment, removing impurities by using a 40-80-mesh vibrating screen, and crushing the dried bean raw material at a low temperature of below 60 ℃ into chips with the particle size of 80-200 meshes to obtain kidney bean powder;

s3, extraction processing, which comprises the following steps:

1, grinding the kidney bean powder obtained in the step S2 into slurry, extracting for 3-5h at the temperature of 40-50 ℃ according to the material-liquid ratio of 1:25-30, and then carrying out separation treatment to obtain precipitate and filtrate which mainly contain starch and fiber;

a2, extracting the precipitate obtained in the step a1 for the second time to obtain protein, wherein the ratio of material to liquid is 1:15-20, extracting for 2-5h at the temperature of 40-50 ℃, and then separating to obtain filtrate;

a3, combining the two filtrates obtained in the separation treatment in the steps a1 and a2, and carrying out separation treatment again to separate out soluble protein and micromolecular sugar;

a4, and mixing the soluble protein obtained in the step a3 with a mixture of 1:20, diluting with purified water, and decolorizing with active carbon column;

a5, adsorbing sugar molecules and nucleic acid molecules again by the decolorized solution through an adsorption column, and further purifying the selenoprotein;

a6, dialyzing and concentrating the decolored solution by using a molecular membrane to remove free micromolecular substances;

a7, concentrating the dialyzed concentrated solution under vacuum and reduced pressure to 30 Baume degree, and freeze-drying or spray-drying at low temperature.

Preferably, the seed collected in step S1 is characterized by: uniform size, and is full and healthy.

Preferably, the separation treatment in the steps a1 and a2 is performed by using a tube centrifuge with the separation factor of 15000G.

Preferably, the separation treatment in the step a3 is carried out by using a tubular centrifuge with a separation factor of 19000G.

Preferably, the adsorption column treatment in the step a5 is carried out by using a No. 50 macroporous resin adsorption column.

Preferably, the pore size of the molecular membrane in the step a6 is 0.001 micron.

(III) advantageous effects

The invention provides a method for preparing water-soluble selenoprotein. Compared with the prior art, the method has the following beneficial effects:

(1) the production and preparation method of the water-soluble selenoprotein aims at local high-quality characteristic resources, selenium-rich kidney beans which are rich in 400ppm of organic selenium and have the total protein content of more than 20 percent are obtained by taking selenium-rich soybeans as raw materials through a further selenium-rich culture technology, the selenium-rich kidney bean is used as a raw material, the modern biotechnology is comprehensively applied, a series of biochemical methods are adopted to enable organic selenium protein stored in the soybean to be dissociated, and then water-soluble selenium protein is prepared through the technological processes of separation, purification, drying and the like, so that the nontoxic and efficient bioactive selenium raw material is provided for the fields of food, medicine, health care products, planting industry, breeding industry and the like, the extraction process of the invention has no chemical pollution, is green and safe, and the extracted bioactive substances are kept unchanged in a short time, the dissolution of the effective components of the plants can be accelerated by beating, stirring and the like, thereby improving the extraction rate.

(2) The production and preparation method of the water-soluble selenoprotein has the advantages of resisting oxidation and delaying senility; improving the immune function of the human body; preventing and treating cardiovascular and cerebrovascular diseases; protecting and repairing cells; preventing and treating eye diseases; toxin removal; inhibiting cancer, and protecting liver.

(3) The kidney bean used in the method for producing and preparing the water-soluble selenoprotein has higher protein content, the amino acid content of the protein is basically higher than a standard value compared with an ideal protein recommended by the Food and Agriculture Organization (FAO) of the United nations, meanwhile, the total amount of the amino acid is up to more than 85 percent, and simultaneously, the potassium content and the magnesium content of the kidney bean are high and the sodium content of the kidney bean is low, so the kidney bean is particularly suitable for being eaten by special patients with selenium deficiency, heart disease, hypokalemia and the like.

Drawings

FIG. 1 is a flow chart of the present invention;

FIG. 2 is a chromatogram of a mixed standard solution of selenium form according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-2, the embodiment of the present invention provides three technical solutions: a method for producing and preparing water-soluble selenoprotein specifically comprises the following embodiments: