阅读说明：本技术 使用磁阻生物传感器阵列表征dna甲基化 (Characterization of DNA methylation using magnetoresistive biosensor arrays ) 是由 李政録 S·X·王 M·F·汉森 M·杜伏威 G·利兹 于 2018-05-01 设计创作，主要内容包括：一种甲基化检测的方法提供了DNA链中甲基化密度的定量描述。亚硫酸氢盐转化[100]含有甲基化和未甲基化位点的DNA链产生具有错配碱基对的转化DNA链。转化DNA链经PCR扩增[102],并用固定于磁阻(MR)传感器阵列上的互补DNA磁性标记[104]和杂交[106]单链靶DNA链。杂交期间,可以记录结合信号。增加诸如温度或盐浓度之类的严格条件[108],以使磁性标记的单链靶DNA链从固定于磁阻(MR)传感器阵列上的互补DNA链变性。严格条件增加期间,实时记录产生自变性的磁性标记的单链靶DNA链的变性信号[110]并用于确定甲基化和未甲基化的DNA链的严格条件[112]。DNA链可能还包含野生型基因和突变的基因,因此突变位点可以与甲基化位点同时确定。(One method of methylation detection provides a quantitative description of the density of methylation in a DNA strand. Bisulfite conversion [100] of a DNA strand containing methylated and unmethylated sites produces a converted DNA strand with mismatched base pairs. The transformed DNA strand is amplified by PCR [102] and magnetically labeled [104] with complementary DNA immobilized on a Magnetoresistive (MR) sensor array and hybridized [106] to a single stranded target DNA strand. During hybridization, the binding signal can be recorded. Stringent conditions such as temperature or salt concentration are increased [108] to denature the magnetically-labeled single-stranded target DNA strands from complementary DNA strands immobilized on a Magnetoresistive (MR) sensor array. During the increase in stringency conditions, the denaturation signal generated from denatured, magnetically-labeled, single-stranded target DNA strands is recorded in real time [110] and used to determine the stringency conditions of methylated and unmethylated DNA strands [112 ]. The DNA strand may also comprise a wild-type gene and a mutated gene, so that the mutation site can be determined simultaneously with the methylation site.)